Comparison of Lipid Content in Nine Dinoflagellate Species Using Flow Cytometry.

The lipid content of nine dinoflagellates was analyzed using flow cytometry to compare lipid levels. Additionally, the correlation between lipid content, cell size, and carbon content in dinoflagellates was evaluated using BODIPY 505/515 staining. The flow cytometry side scatter (SSC) effectively represented relative cell size, showing a linear relationship with the equivalent spherical diameter (ESD).

Probiotic Potential and Enhanced Adhesion of Fermented Foods-Isolated Lactic Acid Bacteria to Intestinal Epithelial Caco-2 and HT-29 Cells.

This study evaluated the probiotic potential of lactic acid bacteria (LAB) isolated from fermented milk and soymilk products purchased from local markets. The LAB strains were assessed for acid and bile resistance, antibiotic resistance, and adhesion to human intestinal epithelial models. (JAMI_LB_02) and (JAMI_LB_05) showed the highest survival rates in artificial gastric and bile juices, at 87.

Evaluation of a Commercial Multiplex Real-Time PCR with Melting Curve Analysis for the Detection of Complex and Five Nontuberculous Mycobacterial Species.

Background: Accurate and timely diagnosis of mycobacterial infections, including complex (MTBC) and nontuberculous mycobacteria (NTM), is crucial for effective disease management.

Methods: This study evaluated the performance of the NeoPlex TB/NTM-5 Detection Kit (NeoPlex assay, Seongnam, Republic of Korea), a multiplex real-time PCR assay that incorporates melting curve analysis, compared with the line-probe assay (LPA). The NeoPlex assay could simultaneously detect and differentiate MTBC from five other NTM species: , , , , and .

Optimization of Medium Composition and Fluidized Bed Drying Conditions for Efficient Production of Dry Yeast.

Yeast formulations such as dry yeast are essential for supplying microbial starters to the alcoholic beverage industry. In Korea, the expensive freeze-drying method is used to manufacture brewer's dry yeast, and therefore an economical process such as fluidized bed drying is needed. In the dry yeast manufacturing process, the medium and drying conditions are key factors that determine its quality and manufacturing cost.

Measurement and Analysis of Interconnects' Resonance and Signal/Power Integrity Degradation in Glass Packages.

In this article, resonance phenomena of high-speed interconnects and power delivery networks in glass packages are measured and analyzed. The resonances are generated in the interconnection by the physical dimension, cancelation of reactance components, and modes. When the resonances are generated in the operation frequency band, the signal/power integrity of the interconnect can be affected.

Temperature-Responsive Hybrid Composite with Zero Temperature Coefficient of Resistance for Wearable Thermotherapy Pads.

Carbon-based polymer composites are widely used in wearable devices due to their exceptional electrical conductivity and flexibility. However, their temperature-dependent resistance variations pose significant challenges to device safety and performance. A negative temperature coefficient (NTC) can lead to overcurrent risks, while a positive temperature coefficient (PTC) compromises accuracy.

Recent Advances in Electromagnetic Devices: Design and Optimization.

Electromagnetic devices are a continuous driving force in cutting-edge research and technology, finding applications in diverse fields such as optics [...

W-Band GaAs pHEMT Power Amplifier MMIC Stabilized Using Network Determinant Function.

This paper presents a W-band power amplifier monolithic microwave integrated circuit (MMIC) that is designed for high-precision millimeter-wave systems and fabricated using a 0.1 µm GaAs pHEMT process. The amplifier's stability was evaluated using the network determinant function, ensuring robust performance under both linear and nonlinear conditions.

Editorial Perspective: Advancements in Microfluidics and Biochip Technologies.

Microfluidics and biochip technologies continue to play a key role in driving innovation across biomedical, environmental and engineering disciplines [...

Preamble Design and Noncoherent ToA Estimation for Pulse-Based Wireless Networks-on-Chip Communications in the Terahertz Band.

The growing demand for high-speed data transfer and ultralow latency in wireless networks-on-chips (WiNoC) has spurred exploration into innovative communication paradigms. Recent advancements highlight the potential of the terahertz (THz) band, a largely untapped frequency range, for enabling ultrafast tera-bit-per-second links in chip multiprocessors. However, the ultrashort duration of THz pulses, often in the femtosecond range, makes synchronization a critical challenge, as even minor timing errors can cause significant data loss.

Designs of Charge-Balanced Edge Termination Structures for 3.3 kV SiC Power Devices Using PN Multi-Epitaxial Layers.

We demonstrated 3.3 kV silicon carbide (SiC) PiN diodes using a trenched ring-assisted junction termination extension (TRA-JTE) with PN multi-epitaxial layers. Multiple P rings and width-modulated multiple trenches were utilized to alleviate electric-field crowding at the edges of the junction to quantitively control the effective charge (Q) in the termination structures.

Slurry Synthesis and Thin-Film Fabrication Toward Production of Li₂O-B₂O₃-Al₂O₃-Based Multilayer Oxide Solid-State Batteries for Internet of Things Applications.

Developing thin-film sheets made of oxide-based solid electrolytes is essential for fabricating surface-mounted ultracompact multilayer oxide solid-state batteries. To this end, solid-electrolyte slurry must be optimized for excellent dispersibility. Although oxide-based solid electrolytes for multilayer structures require sintering, high processing temperatures cause problems such as Li-ion volatilization and reactions with graphite anodes.

Inchworm Robots Utilizing Friction Changes in Magnetorheological Elastomer Footpads Under Magnetic Field Influence.

The application of smart materials in robots has attracted considerable research attention. This study developed an inchworm robot that integrates smart materials and a bionic design, using the unique properties of magnetorheological elastomers (MREs) to improve the performance of robots in complex environments, as well as their adaptability and movement efficiency. This research stems from solving the problem of the insufficient adaptability of traditional bionic robots on different surfaces.

Substrate Integrated Waveguide on Glass with Vacuum-Filled Tin Through Glass Vias for Millimeter-Wave Applications.

This paper presents a novel approach to fabricate substrate integrated waveguides (SIWs) on glass substrates with tin (Sn) through glass vias (TGVs) tailored for millimeter-wave applications. The fabrication process employs a custom-designed vacuum suctioning system to rapidly fill precise TGV holes in the glass substrate, which are formed by wafer-level glass reflow micromachining techniques with molten tin in a minute. This method offers a very fast and cost-effective alternative for complete via filling without voids compared to the conventional metallization techniques such as electroplating or sputtering.

Evaluation of Genetic Diversity and Identification of Cultivars in Spray-Type Chrysanthemum Based on SSR Markers.

Background/objectives: Chrysanthemum (), a key ornamental and medicinal plant, presents challenges in cultivar identification due to high phenotypic similarity and environmental influences. This study assessed the genetic diversity and discrimination of 126 spray-type chrysanthemum cultivars.

Methods: About twenty-three simple sequence repeat (SSR) markers were screened for the discrimination of 126 cultivars, among which six SSR markers showed polymorphic fragments.

Compound Heterozygous p.(R124C) (Classic Lattice Corneal Dystrophy) and p.(R124H) (Granular Corneal Dystrophy Type 2) in : Phenotype, Genotype, and Treatment.

(1) Background: The phenotypes of classic lattice corneal dystrophy (LCD) and granular corneal dystrophy type 2 (GCD2) that result from abnormalities in gene () have previously been described. The phenotype of compound heterozygous classic LCD and GCD2, however, has not yet been reported. (2) Case report: A 39-year-old male (proband) presented to our clinic complaining of decreased vision bilaterally.

Comprehensive Analysis of the NHX Gene Family and Its Regulation Under Salt and Drought Stress in Quinoa ( Willd.).

: Abiotic stresses such as salinity and drought significantly constrain crop cultivation and affect productivity. Quinoa ( Willd.), a facultative halophyte, exhibits remarkable tolerance to drought and salinity stresses, making it a valued model for understanding stress adaptation mechanisms.

Mutations Causing X-Linked Recessive Oligodontia with Variable Expression.

Background/objectives: The ectodysplasin A () gene, a member of the tumor necrosis factor ligand superfamily, is involved in the early epithelial-mesenchymal interaction that regulates ectoderm-derived appendage formation. Numerous studies have shown that mutations in the gene can cause X-linked ectodermal dysplasia (ED) and non-syndromic oligodontia (NSO). Accordingly, this study aimed to identify the causative genetic mutations of the gene.

Complete Mitochondrial Genome of (Perciformes: Niphonidae): Genome Characterization and Phylogenetic Analysis.

The species (Cuvier, 1829) is the only representative of the family Niphonidae and the genus , and its taxonomic history is complicated; it is still unclear in a phylogenetic sense. In this study, we report the complete mitochondrial genome of (OP391482), which was determined to be 16,503 bp long with biased A + T contents (53.8%) using next-generation technology.

C/EBPβ Regulates HIF-1α-Driven Invasion of Non-Small-Cell Lung Cancer Cells.

Metastatic cancer accounts for most cancer-related deaths, and identifying specific molecular targets that contribute to metastatic progression is crucial for the development of effective treatments. Hypoxia, a feature of solid tumors, plays a role in cancer progression by inducing resistance to therapy and accelerating metastasis. Here, we report that CCAAT/enhancer-binding protein beta (C/EBPβ) transcriptionally regulates () and thus promotes migration and invasion of non-small-cell lung cancer (NSCLC) cells under hypoxic conditions.

Pharmacodynamic Profiling of Amoxicillin: Targeting Multidrug-Resistant Gram-Positive Pathogens and in Canine Clinical Isolates.

The rising threat of antimicrobial resistance (AMR) is a global concern in both human and veterinary medicine, with multidrug-resistant (MDR) pathogens such as and presenting significant challenges. : This study evaluates the effectiveness of amoxicillin against these MDR pathogens in canine isolates using pharmacokinetic and pharmacodynamic parameters. : Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and mutation prevention concentration (MPC) were assessed.

Characterization of Phage Endolysin PlyDolk21.

is a significant cause of food poisoning. Broad-spectrum antibiotics, commonly used to control , are becoming less effective due to the rise of antibiotic-resistant strains, necessitating alternative control strategies. A -infecting bacteriophage, Dolk21, and its endolysin, PlyDolk21, were isolated and characterized.

Bacteria-Inspired Synthesis of Silver-Doped Zinc Oxide Nanocomposites: A Novel Synergistic Approach in Controlling Biofilm and Quorum-Sensing-Regulated Virulence Factors in .

Multidrug-resistant infections pose a critical challenge to healthcare systems, particularly in nosocomial settings. This drug-resistant bacterium forms biofilms and produces an array of virulent factors regulated by quorum sensing. In this study, metal-tolerant bacteria were isolated from a metal-contaminated site and screened for their ability to synthesize multifunctional nanocomposites (NCs).

Inhibitory Effect of Seed Extract and Its Behenic Acid Component on Biofilm Formation.

Inhibiting biofilm formation without killing cells facilitates the physical removal of contaminating bacteria while minimizing the opportunity for resistant bacteria to emerge. The . methanolic seed extract contained 1.

Standardization of the Agar Plate Method for Bacteriophage Production.

The growing threat of antimicrobial resistance (AMR), exacerbated by the COVID-19 pandemic, highlights the urgent need for alternative treatments such as bacteriophage (phage) therapy. Phage therapy offers a targeted approach to combat bacterial infections, particularly those resistant to conventional antibiotics. This study aimed to standardize an agar plate method for high-mix, low-volume phage production, suitable for personalized phage therapy.