Inefficient uptake of small interfering RNAs is responsible for their inability to trigger RNA interference in Colorado potato beetle cells.

There has been limited success in the usage of exogenous small interference RNA (siRNA) or small hairpin RNA (shRNA) to trigger RNA interference (RNAi) in insects. Instead, long double-stranded RNAs (dsRNA) are used to induce knockdown of target genes in insects. Here, we compared the potency of si/sh RNAs and dsRNA in Colorado potato beetle (CPB) cells.

Polyol-assisted hydrothermal synthesis of Mn-doped α - FeO(MFO) nanostructures: Spin disorder-induced magnetism and photocatalytic properties.

Hierarchical nanostructures play an important role in environmental clean-up and sustainability applications. The magnetic and photocatalytic characteristics of flower-like Mn-doped α-FeO nanostructures were prepared by using a polyol-assisted hydrothermal method. Crystallite sizes are in the range of 35-42 nm, and the existence of 3D hierarchical nanostructures was observed in FESEM pictures.

Green synthesis of multifunctional carbon quantum dots: An approach in cancer theranostics.

Carbon quantum dots (CQDs) have gained significant growing attention in the recent past due to their peculiar characteristics including smaller size, high surface area, photoluminescence, chemical stability, facile synthesis and functionalization possibilities. They are carbon nanostructures having less than 10 nm size with fluorescent properties. In recent years, the scientific community is curiously adopting biomass precursors for the preparation of CQDs over the chemical compounds.

Photoprotective Potential, Cytotoxicity, and UPLC-QTOF/MS Analysis on Bioactive Solvent Fractions of Nimmo Bark.

Nimmo (Moringaceae) belongs to the same family of (miracle tree) and is a medicinal plant traditionally used by Indians to treat various ailments related to diabetes, tumours, inflammation, and blood pressure. Despite its versatility, the photoprotective properties of the plant remain unclear. This study revealed the UV-protective properties of its methanol bark extract and respective subfractions, chloroform, hexane, and ethyl acetate through total phenolic and flavonoid content (TPC & TFC), antioxidant (DPPH), sun protecting factor (SPF) value, and UV absorption spectra analysis.

Antioxidant and Antivenom Potential of an Essential Oil, 4-(2-Oxo-propyl)-cyclopentane-1,3-dione, and Allantoin Derived from the Polyherbal Combination of L. and L.

The goal of this study was to identify new compounds from a methanol extract of a polyherbal combination of L. and L. (MECAIPN), two traditional medicinal plants used to cure envenomation, as well as to assess their antioxidant and antivenom properties.

Therapeutic Potential of Cucumis melo (L.) Fruit Extract and Its Silver Nanopartciles Against DEN-Induced Hepatocellular Cancer in Rats.

Biosynthesized silver nanoparticles have a wide range of biological activities and using nanoparticles as one of the novel approaches in cancer therapy. In this present research work, the anti-cancer efficacy of Cucumis melo fruit extract and its silver nanoparticles was explored. Wistar rats were divided into six groups and hepatic cancer was induced with 0.

A Novel Rhizospheric Bacterium: Bacillus velezensis NKMV-3 as a Biocontrol Agent Against Alternaria Leaf Blight in Tomato.

A novel strain of Bacillus isolated from rhizosphere has shown to be an excellent biocontrol agent against various plant pathogens. In this study, a first report of a Bacillus strain NKMV-3 which effectively controls Alternaria solani, which cause the early blight disease in tomato. Based on the cultural and molecular sequencing of 16S rRNA gene sequence, the identity of the strain was confirmed as Bacillus velezensis NKMV-3.

Prodigiosin production from Serratia marcescens strain CSK and their antioxidant, antibacterial, cytotoxic effect and in silico study of caspase-3 apoptotic protein.

The present study emphasizes the production and optimization of prodigiosin (PG) pigment from Serratia marcescens strain CSK, which was isolated from Shevaroy Hills, Salem district, Tamil Nadu, India. The response surface methodology analysis was applied for the optimization process of PG production. The maximum production of PG (2950 mg/L) was obtained at pH 7.

A sustainable green synthesis of functionalized biocompatible carbon quantum dots from Aloe barbadensis Miller and its multifunctional applications.

Herein, we demonstrated a sustainable green approach for the preparation of fluorescent biocompatible carbon quantum dots by microwave-assisted reflux synthesis from Aloe barbadensis Miller (Aloe vera) extract. The Transmission Electron Microscopic images reveal that the as-prepared CQDs are spherical with less than 5 nm in size. The CQDs are amorphous, showed an excitation-independent behaviour, emitted blue fluorescence and have a fluorescence quantum yield of 31%.

Microwave-assisted green synthesis of fluorescent carbon quantum dots from Mexican Mint extract for Fe detection and bio-imaging applications.

Biomass-derived carbon quantum dots have drawn special interest owing to their admirable photostability, biocompatibility, fluorescence, high solubility, sensitivity and environmentally friendly properties. In the present work, the Carbon Quantum Dots (CQDs) was synthesized from the Plectranthus amboinicus (Mexican Mint) leaves via the microwave-assisted reflux method. The strong absorption peaks observed from UV-vis spectra at 291 and 330 nm corresponds to the π-π* and n-π* transitions, respectively, reveal the formation of CQDs.

Mathematical model for spreading of COVID-19 virus with the Mittag-Leffler kernel.

In the Nidovirales order of the Coronaviridae family, where the coronavirus (crown-like spikes on the surface of the virus) causing severe infections like acute lung injury and acute respiratory distress syndrome. The contagion of this virus categorized as severed, which even causes severe damages to human life to harmless such as a common cold. In this manuscript, we discussed the SARS-CoV-2 virus into a system of equations to examine the existence and uniqueness results with the Atangana-Baleanu derivative by using a fixed-point method.

Vermitransformation of monogastric Elephas maximus and ruminant Bos taurus excrements into vermicompost using Eudrilus eugeniae.

Biotransformation of monogastric (Elephas maximus) and ruminant (Bos taurus) excrements with Eudrilus eugeniae was assessed by establishing five different treatments in triplicate: 100% elephant dung (T1), 50% elephant dung + 50% garden soil (T2), 50% elephant dung + 50% cow dung (T3), 100% cow dung (T4) and 50% cow dung + 50% garden soil (T5) and maintained for 90 days under experimental conditions. An increment of macronutrients and reduction of C/N ratio (<20) in the vermicomposts was recorded, where T3 presented higher NPK contents with the relative nutrient recovery efficiency of 1.65, 2.

systemic RNA interference defective protein 1 enhances RNAi efficiency in a lepidopteran insect, the fall armyworm, in a tissue-specific manner.

RNA interference (RNAi) is an important tool for gene function studies in insects, especially in non-model insects. This technology is also being developed for pest control. However, variable RNAi efficiency among insects is limiting its use in insects.

Nutrient recovery and vermicompost production from livestock solid wastes with epigeic earthworms.

The aim of this work was to study nutrient recovery and vermicompost production from livestock solid wastes of indigenous and exotic cow breeds with epigeic earthworms. Fourteen days pre-decomposed dung of Vechur native (CD1) and exotic Jersey (CD2) breeds were vermicomposted with Perionyx excavatus and Eudrilus eugeniae for 45 days (Cycle I) and 90 days (Cycle II) including respective controls without earthworms. Vermicomposts from CD1 and CD2 substrates during Cycle I and II showed increase in NPK, Ca and micro-nutrients (P < 0.

Evaluation of probiotic against foodborne pathogens and its fermentation potential in improving silage quality.

The objective of this study was to isolate the lactic acid bacteria from fermented silage sample and analyze their antibacterial activities, probiotic properties, and fermentation potential in silage. Eleven lactic acid bacteria (LAB) were selected based on distinct morphologies and preliminary studies. Cell-free supernatant (CFS) was then prepared from the selected strains for antibacterial analysis.

Influence of arbuscular mycorrhizal fungi on biochemical changes in wedilla chinensis (osbeck) merril.

A greenhouse experiment was conducted in order to evaluate the efficiency of seven Arbuscular Mycorrhizal Fungi (AMF) namely, Acaulospora deligata, Glomus aggregatum, G feugianum, G fasciculatum, G rubiforme, Gigaspora margarita, and Scutellospora heterogama collected from the rhizosphere soils of Wedilia chinensis (Osbeck) Merril. They were evaluated on the same host for improvement of phytochemical constituents, namely total phenols, ortho dihydroxy phenols, flavonoids, alkaloids, tannins and saponins and enhanced levels of acid and alkaline phosphatase, nitrate reductase, peroxidase and polyphenol oxidase activities. The extent of phytochemical constituents and levels of enzymes varied with the species of AM fungi inhabiting the W.

Pharmacognostical and phytochemical studies on Origanum vulgare L.(Laminaceae).

With increasing demand in the field of herbal medicines and cosmetics, it has become necessary and pertinent to probe into the area of systematic knowledge about herbal drugs. There is a need for the application of this knowledge in authentification, detailed study and practical utilization of crude drugs. The present paper deals with the taxonomy, anatomy, powder study pertaining to organoleptic, microscopic, fluorescence, physical constant evaluations and phytochemical screening of Origanum vulgare L.