1,474 results match your criteria: "Kobe Pharmaceutical University[Affiliation]"

The basic requirements for the development of radiopharmaceuticals for radionuclide therapy of tumors include marked tumor-specific accumulation and long-term intratumoral retention. We have previously reported an indium-111 (In)-labeled thermoresponsive polymer (polyoxazoline (POZ)) that is soluble at body temperature with rapid clearance from normal tissues but self-aggregates in the tumor upon tumor heating treatment. POZ accumulated in the tumor via self-aggregation under hyperthermic conditions and was retained after stopping heat exposure.

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Effect of N-glycosylation on secretion, degradation and lipoprotein distribution of human serum amyloid A4.

Biochim Biophys Acta Mol Cell Biol Lipids

December 2024

Laboratory of Functional Molecular Chemistry, Kobe Pharmaceutical University, Kobe 658-8558, Japan. Electronic address:

Serum amyloid A (SAA) is a family of apolipoproteins predominantly synthesized and secreted by the liver. Human SAA4 is constitutively expressed and contains an N-glycosylation site that is not present in other SAA subtypes. SAA4 proteins are not fully glycosylated, resulting in the presence of both glycosylated and non-glycosylated forms in human plasma.

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In vivo systemic evaluation of nasal drug absorption from powder formulations in rats.

Eur J Pharm Biopharm

December 2024

Laboratory of Pharmaceutical Technology, Kobe Pharmaceutical University, 4-19-1 Motoyamakitamachi, Higashinada-ku, Kobe, Hyogo 658-8558, Japan.

Despite the potential benefits of nasal drug delivery, there is a need for a systematic evaluation of the efficacy of powder formulations adhering to the nasal mucosa. This study aims to establish a systematic evaluation method for nasal drug absorption from powder formulations. We selected three model compounds-antipyrine, griseofulvin, and acyclovir-and analyzed their pharmacokinetics following nasal administration of powder formulations under physiological conditions.

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Although some case reports have reported gastrointestinal perforation after COVID-19, epidemiological studies investigating this association are scarce. We aimed to assess the risk of gastrointestinal perforation associated with COVID-19. We conducted a self-controlled case series (SCCS) study using a Japanese nationwide, large-scale claims database.

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The World Health Organization (WHO) recommends that countries adopt the reference level of 100 Bq m-3 for indoor radon gas. Adopting the reference level requires a preliminary assessment of the indoor radon concentration. In the preliminary investigation, a cut-off value is useful, which is a value for selecting samples that can be reliably determined to be below the reference level (in this paper, the WHO reference level) using a straightforward method.

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Lipid bilayer vesicles, liposomes are representative drug delivery carriers. High encapsulation efficiency and release control of drugs are essential for clinical application of liposomes. For efficient drug loading into liposomes, remote loading method using driving force like transmembrane gradients of pH and ions are utilized.

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Aims: Neudesin, a heme-binding protein previously identified for its neurotrophic activity, has been implicated in various physiological and pathological processes, including immune regulation. However, its role in inflammatory macrophages remains unclear. Herein, we investigated the function of neudesin in the regulation of inflammatory macrophages.

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Although hypertension is a major cause of cardiovascular disease, the control of blood pressure (BP) is insufficient worldwide. Exercise is an effective treatment for reducing BP, but the differences in the blood pressure lowering effects of exercise according to the underlying pathophysiological condition, the type of exercise, and the geographic region are not fully understood. An umbrella review with a meta-analysis of 435 randomized controlled trials that investigated the BP-lowering effects of exercise was performed using Ovid MEDLINE and the Cochrane Library, covering the period from inception to August 1, 2023.

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In this study, thermosensitive liposomes (TSLs) encapsulating urinary excretable radiolabeled compounds were developed. We considered that the release of the radiolabeled compounds from the TSLs in the blood by heating the blood in peripheral tissues can achieve rapid clearance of radioactivity, resulting in improved imaging contrast. To demonstrate the hypothesis, classical TSLs mainly composed of 1,2-dipalmitoyl--glycero-3-phosphocholine with a phase transition temperature of 41 °C were used.

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Synergistic effect of cyclodextrins and electrolytes at high concentrations on protein aggregation inhibition.

J Pharm Sci

December 2024

Laboratory of Functional Molecular Chemistry, Kobe Pharmaceutical University, 4-19-1, Motoyamakita-machi, Higashinada-ku, Kobe 658-8558, Japan.

The stabilization of protein therapeutics against aggregation is crucial for maintaining their efficacy and safety. This study investigated the synergistic effects of cyclodextrins (CDs) and electrolytes at high concentrations on the stabilization of immunoglobulin G (IgG), insulin, and adeno-associated virus (AAV) vectors. The effects of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) combined with various electrolytes were evaluated using human plasma-derived IgG as a model protein.

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Article Synopsis
  • * Neudesin, which is highly expressed in human tumors, suppresses DC function, and lower levels of it in melanoma patients correlate with better immune responses and treatment outcomes.
  • * In mouse models, lacking neudesin led to slower tumor growth and a stronger immune response, suggesting neudesin could be a potential target for improving cancer immunotherapy.
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Detection of lipid radicals generated via cerebral ischemia/reperfusion injury using a radiolabeled nitroxide probe.

Free Radic Biol Med

November 2024

Laboratory of Biophysical Chemistry, Kobe Pharmaceutical University, 4-19-1 Motoyamakita-machi, Higashinada-ku, Kobe, 658-8558, Japan. Electronic address:

Reactive oxygen species generated via reperfusion cause lipid damage and induce lipid peroxidation, leading to cerebral ischemia/reperfusion injury and exacerbation of cerebral infarction. Lipid radicals are key molecules generated during lipid peroxidation. Therefore, understanding the spatiotemporal behavior of lipid radicals is important to improve the therapeutic outcomes of cerebral infarction.

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Article Synopsis
  • Despite improvements in diabetes care, new treatments are needed to effectively stop the worsening of diabetic kidney disease (DN-DKD), highlighting the significance of endothelin-1 in this condition.
  • Endothelin-1 contributes to kidney damage by promoting cell proliferation and causing injury to podocytes, with receptor antagonists like atrasentan showing promise in protecting the kidneys by lowering protein levels and damage.
  • The review discusses how these new drugs could potentially slow down cDN-DKD's progression while considering possible side effects, like fluid retention and heart failure, and the importance of combining them with current standard treatments.
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Background: Valganciclovir (VGCV) is administered at a dose of 16 mg/kg 2 times daily for 6 months to treat symptomatic congenital cytomegalovirus (CMV) infections. During the treatment period, approximately 20% of the patients developed grade 3 or higher neutropenia. Currently, information on the pharmacokinetics and pharmacodynamics of ganciclovir, an active metabolite of VGCV, in infants is limited.

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Even with recent advances in pathobiology and treatment options, chronic obstructive pulmonary disease (COPD) remains a major contributor to morbidity and mortality. To develop new ways of combating this disease, breakthroughs in our understanding of its mechanisms are sorely needed. Investigating the involvement of underanalyzed lung cell types, such as endothelial cells (ECs), is one way to further our understanding of COPD.

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Na-K-2Cl cotransporter-1 (NKCC1) is present in brain cells, including astrocytes. The expression of astrocytic NKCC1 increases in the acute phase of traumatic brain injury (TBI), which induces brain edema. Endothelin-1 (ET-1) is a factor that induces brain edema and regulates the expression of several pathology-related genes in astrocytes.

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Gold nanorods (GNR) produce heat upon irradiation with near-infrared light, enabling a tumor-targeted photothermal therapy. In this study, we prepared GNR coated with sulfated hyaluronic acid (sHA) with a binding affinity for CD44 via electrostatic interactions to deliver GNR to tumors efficiently and stably, and evaluated their usefulness for photothermal therapy. Cationic GNR modified with trimethylammonium groups electrostatically interacted with native HA or sHA with varying degrees of sulfation to form complexes.

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Apocarotenoids have short carbon chain structures cleaved at a polyene-conjugated double bond. They can be biosynthesized in plants and microorganisms. Animals ingest carotenoids through food and then metabolize them into apocarotenoids.

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Development of a Pyrone-Fused Tricyclic Scaffold-based Ratiometric Fluorescent Probe for Al Detection.

J Fluoresc

July 2024

Laboratory of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, 11-68 Koshien Kyubancho, Nishinomiya, 663-8179, Japan.

Aluminum (Al) is environmentally abundant and can harm living organisms in various ways, such as by inhibiting root growth, damaging faunal nervous systems, and promoting tumor cell proliferation. However, the dynamics of Al in living organisms are largely unknown; thus, detecting Al in the environment and organisms is crucial. Fluorescent probes are useful tools for the selective detection of metal ions.

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Sonic hedgehog (Shh) is a secreted glycopeptide belonging to the hedgehog family that is essential for morphogenesis during embryonic development. The Shh signal is mediated by two membrane proteins, Patched-1 (Ptch-1) and Smoothened (Smo), following the activation of transcription factors such as Gli. Shh decreases the permeability of the blood-brain barrier (BBB) and plays a key role in its function.

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BACH1 inhibits senescence, obesity, and short lifespan by ferroptotic FGF21 secretion.

Cell Rep

July 2024

Department of Biochemistry, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980-8575, Japan; Center for Regulatory Epigenome and Diseases, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980-8575, Japan. Electronic address:

Article Synopsis
  • Ferroptosis is a regulated form of cell death driven by iron and lipid damage, which can be induced in mouse embryonic fibroblasts by the transcription factor BACH1.
  • This process leads to increased secretion of the longevity factor FGF21, promoting the growth of certain cells and reducing signs of aging, shown by the suppression of senescence in other cells.
  • BACH1 enhances FGF21 expression by managing its degradation and, in experiments, this secreted FGF21 helps combat obesity and the accelerated aging in mice with progeria.
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Introduction: Allergic diseases, such as anaphylaxis and urticaria, pose significant health concerns. The quest for improved prognostic outcomes in these diseases necessitates the exploration of novel therapeutic avenues. To address this need, we have developed a novel mouse model of anaphylaxis, denoted as anaphylaxis-dependent spotted distribution of immune complex in skin (ASDIS).

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