87 results match your criteria: "Kobe City Eye Hospital[Affiliation]"
Invest Ophthalmol Vis Sci
November 2024
Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe, Japan.
Lancet
November 2024
Kobe City Eye Hospital, Chuo-ku, Kobe, Hyogo 650-0047, Japan. Electronic address:
SLAS Technol
December 2023
Kobe City Eye Hospital. 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047 Japan; Robotic Biology Institute Inc. Telecom Center Building East Wing 1F, 2-5-10 Aomi, Koto-ku, Tokyo 135-0064 Japan; Laboratory for Biologically Inspired Computing, RIKEN Center for Biosystems Dynamics Research. 6-2-3 Furuedai, Suita, Osaka 565-0874 Japan; Vision Care Inc. Kobe Eye Center Building 5F, 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047 Japan. Electronic address:
The consistent production of high-quality cells in cell therapy highlights the potential of automated manufacturing. Humanoid robots are a useful option for transferring technology to automate human cell cultures. This study evaluated a robotic cell-processing facility (R-CPF) for clinical research on retinal cell therapy, incorporating the versatile humanoid robot Maholo LabDroid and an All-in-One CP unit.
View Article and Find Full Text PDFSLAS Technol
December 2024
Laboratory Automation Suppliers' Association, 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047 Japan; Laboratory for Biologically Inspired Computing, RIKEN Center for Biosystems Dynamics Research, 6-7-1 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047 Japan; Kobe City Eye Hospital, 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047 Japan. Electronic address:
Invest Ophthalmol Vis Sci
October 2024
RIKEN Center for Biosystems Dynamics Research, Laboratory for Retinal Regeneration, Minato-jima, Chuo-ku, Kobe, Hyogo, Japan.
Purpose: Retinitis pigmentosa represents a leading cause of blindness in developed countries, yet effective treatments for the disease remain unestablished. Previous studies have demonstrated the potential of stem cell-derived retinal organoid (SC-RO) sheet transplantation to form host-graft synapses and to improve light responsiveness in animal models of retinal degeneration. However, the detailed microstructures of these de novo synapses and their functional contribution have not been well elucidated.
View Article and Find Full Text PDFStem Cell Reports
November 2024
Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan; Kobe City Eye Hospital, Kobe, Hyogo 650-0047, Japan. Electronic address:
Macular hole (MH) is a retinal break involving the fovea that causes impaired vision. Although advances in vitreoretinal surgical techniques achieve >90% MH closure rate, refractory cases still exist. For such cases, autologous retinal transplantation is an optional therapy showing good anatomic success, but visual improvement is limited and peripheral visual field defects are inevitable after graft harvesting.
View Article and Find Full Text PDFCommun Med (Lond)
September 2024
Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe, Japan.
Background: Gene editing of immunomodulating molecules is a potential transplantation strategy to control immune rejection. As we noticed the successful transplantation of retinal pigment epithelium (RPE) derived from embryonic stem cells of a cynomolgus monkey that accidentally lacked MHC class II (MHC-II) molecules, we hypothesized immune rejection could be evaded by suppressing MHC-II.
Methods: Gene editing by the Crispr/Cas9 system was performed in induced pluripotent stem cells derived from a cynomolgus monkey (miPSCs) for targeted deletion of the gene coding class II MHC trans-activator (CIITA).
Proc Natl Acad Sci U S A
September 2024
Department of Biological Structure, University of Washington, Seattle, WA 98195.
The retinal fovea in human and nonhuman primates is essential for high acuity and color vision. Within the fovea lies specialized circuitry in which signals from a single cone photoreceptor are largely conveyed to one ON and one OFF type midget bipolar cell (MBC), which in turn connect to a single ON or OFF midget ganglion cell (MGC), respectively. Restoring foveal vision requires not only photoreceptor replacement but also appropriate reconnection with surviving ON and OFF MBCs and MGCs.
View Article and Find Full Text PDFCureus
July 2024
Department of Ophthalmology, Kobe City Eye Hospital, Kobe, JPN.
This study evaluates the transplantation of induced pluripotent stem cell (iPSC)-derived retinal organoids into patients with advanced retinitis pigmentosa using adaptive optics optical coherence tomography (AO-OCT) to monitor retinal changes over two years post transplantation. Our results confirmed successful engraftment and increased retinal thickness, with AO-OCT providing detailed visualization of cellular structures such as an outer plexiform layer-like line and highly reflective particles within rosette-like formations, indicative of photoreceptor development. Immunohistological analysis in a parallel monkey model confirmed these structures as mature, functional photoreceptor rosettes.
View Article and Find Full Text PDFRegen Med
May 2024
Department of Ophthalmology, Kobe City Eye Hospital, 2-1-8, Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
Retina
August 2024
Department of Ophthalmology, Kobe City Eye Hospital, Kobe, Japan.
Neuroscience
August 2024
Department of Ophthalmology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
PLoS One
June 2024
Department of Ophthalmology, Kobe City Eye Hospital, Kobe, Japan.
PLoS One
June 2024
Department of Ophthalmology, Kobe City Eye Hospital, Kobe, Hyogo, Japan.
Inherited retinal dystrophies comprise a clinically complex and heterogenous group of diseases characterized by visual impairment due to pathogenic variants of over 300 different genes. Accurately identifying the causative gene and associated variant is crucial for the definitive diagnosis and subsequent selection of precise treatments. Consequently, well-validated genetic tests are required in the clinical practice.
View Article and Find Full Text PDFElife
May 2024
Laboratory for Retinal Regeneration, RIKEN Biosystems Dynamics Research (BDR), Kobe, Japan.
Retinal pigment epithelium (RPE) cells show heterogeneous levels of pigmentation when cultured in vitro. To know whether their color in appearance is correlated with the function of the RPE, we analyzed the color intensities of human-induced pluripotent stem cell-derived RPE cells (iPSC-RPE) together with the gene expression profile at the single-cell level. For this purpose, we utilized our recent invention, Automated Live imaging and cell Picking System (ALPS), which enabled photographing each cell before RNA-sequencing analysis to profile the gene expression of each cell.
View Article and Find Full Text PDFSci Rep
May 2024
Kobe City Eye Hospital, 2-1-8, Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan.
Am J Ophthalmol
August 2024
Department of Ophthalmology (K.M., T.H., T.N.), The Jikei University School of Medicine, Tokyo, Japan.
Sci Rep
February 2024
Department of Ophthalmology, Kobe City Eye Hospital, 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan.
This study aimed to investigate how the extent and central/peripheral location of the residual visual field (VF) in patients with late-stage inherited retinal diseases (IRDs) are related to retinal sensitivity detected using full-field stimulus testing (FST). We reviewed the results of Goldmann perimetry and FST from the medical records of patients with IRDs whose VF represents central (within 10°) and/or peripheral islands, or undetectable. In total, 19 patients (19 eyes) were analyzed in this study.
View Article and Find Full Text PDFRegen Ther
March 2024
Kobe City Eye Hospital, 2-1-8 Minatojima-minamimachi, Chuo-ku, Kobe, Japan.
Stem Cell Reports
February 2024
Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan; Department of Ophthalmology, Kobe City Eye Hospital, Kobe, Hyogo 650-0047, Japan.
Cureus
December 2023
Department of Ophthalmology, Kobe City Eye Hospital, Kobe, JPN.
We investigated the potential of ChatGPT in the ophthalmological field in the Japanese language using board examinations for specialists in the Japanese Ophthalmology Society. We tested GPT-3.5 and GPT-4-based ChatGPT on five sets of past board examination problems in July 2023.
View Article and Find Full Text PDFObjective: Cystoid macular edema (CME) in retinitis pigmentosa (RP) is an important complication causing visual dysfunction. We investigated the effect of CME on photoreceptors in RP patients with previous or current CME, using an adaptive optics (AO) fundus camera.
Methods: We retrospectively observed the CME and ellipsoid zone (EZ) length (average of horizontal and vertical sections) by optical coherence tomography.
Cell Stem Cell
December 2023
Department of Ophthalmology, Kobe City Eye Hospital, Kobe 650-0047, Japan; Department of Ophthalmology, Kobe City Medical Center General Hospital, Kobe 650-0047, Japan; Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan.
Ophthalmol Sci
December 2023
Department of Ophthalmology, Kobe City Eye Hospital, Kobe, Japan.
Purpose: Novel therapeutic options, such as regenerative medicine and gene therapy, are now emerging as viable treatment options for patients with severe visual impairments, such as retinitis pigmentosa (RP). Gradable assessment of patients' visual function is essential to consider treatment options and to evaluate treatment outcomes; however, evaluation of visual function in patients with advanced low vision is often challenging because of patients' poor and sometimes unpredictable responses. In this study, we attempted to accurately assess visual capabilities and disease stage in patients with RP with a visual acuity (VA) of ≤ 0.
View Article and Find Full Text PDFRetin Cases Brief Rep
September 2023
Department of Ophthalmology, Kobe City Eye Hospital, Kobe, Japan.
Purpose: To describe a novel case of bilateral rapidly progressive retinopathy after immunotherapy with pembrolizumab for metastatic urothelial carcinoma.
Methods: Case report.
Results: A 64-year-old man undergoing pembrolizumab immunotherapy was referred to our hospital because of bilateral acute vision loss.