Modulation of cell surface antigens and regulation of phagocytic activity mediated by CD11b in the monocyte-like cell line U937 in response to lipopolysaccharide.

Modulation of the cellular antigens and regulation of the phagocytic activity of the monocyte-like cell line U937 after culture with lipopolysaccharide (LPS) were investigated. CD14 expression was induced on the surface of the U937 cells after 48 h of culture with LPS and then they became adhesive with numerous filamentous filopodia extruded on the cell surface, exhibiting the enhanced expression of CD16 and CD23, the activation cell surface markers for differentiation into macrophage. However, no induction or enhancement of the cell surface expression was observed with respect to CD11b, CD18, HLA-A, B, C, HLA-DR, DQ, DP or CD57.

[Industrial hygienic study on nursing activities. II. Investigation on heart rate and energy expenditure of ward nurses on shift].

By using a Holter electrocardiograph (ECG), we calculated the heart rates of ward nurses on day shift which included different activities. Energy expenditure was calculated from the heart rate data and nurse work load was evaluated. The following results were obtained.

Industrial hygienic study on nursing activities comparison of energy expenditure between pedometer and Holter electrocardiograph.

Few objective and quantitative studies have been made on energy expenditure during hospital nursing activities. The pedometer is a light handy instrument, which gives highly accurate results, and is a minimal burden for subjects. To ascertain daily energy expenditures, we used a pedometer.

Industrial hygienic study on nursing activities investigation on heart rate and energy expenditure of cranial nerves and ICU ward nurses.

Using a Holter electrocardiograph (ECG), we calculated the heart rates of ward nurses during day shifts, a time in which various activities are undertaken. Energy expenditures were calculated from the heart rate data and nursing workloads were evaluated. The following results were obtained.

[Industrial hygienic study on nursing activities. I. Investigation on heart rate and energy expenditure of ward nurses by nursing activity].

Using a Holter electrocardiograph (ECG), we recorded the heart rates of ward nurses by working hours and nursing activity. Energy expenditure was calculated from the heart rate data and nurse work lord was evaluated. The following results were obtained.

Induction of CD14 antigen on the surface of U937 cells by an interleukin-6 autocrine mechanism after culture with formalin-killed gram-negative bacteria.

In order to better understand the regulation of CD14 antigen on the surface of the monocyte-like cell line U937 in response to bacteria, the expression and regulation of CD14 antigen on these cells when cultured with formalin-killed bacteria were determined using the monoclonal antibody MY-4 and analyzed by means of the indirect immunofluorescence method. CD14 expression was induced on the U937 cells after about 48 hours of culture with all of the formalin-killed Gram-negative bacteria used in this study but with none of the Gram-positive bacteria. Maximum expression was obtained after culture with formalin-killed Salmonella enteritidis strain 116-54.

A cytogenetic survey of 14,835 consecutive liveborns.

The results of chromosome studies on cultured umbilical cord blood lymphocytes from a consecutive series of 14,835 liveborn infants (7,608 males and 7,227 females) are described. Ninety-three infants (6.27 per 1,000) had a major chromosome abnormality.

[Modulation of the surface antigens of Salmonella-phagocytized U937 cells].

In this study, modulation of the surface antigens of Salmonella enteritidis-phagocytized U937 cells and morphology of the bacteria in these cells were analyzed by the indirect immunofluorescence technique. The results are as follows: (1) Morphological studies revealed that the bacteria phagocytized by the U937 cells were transformed to a small coccoid form. (2) The expression of CD14 antigen was observed 24 to 48 h after phagocytosis.

Immunohistochemical localization of basic fibroblast growth factor in A431 human epidermoid carcinoma cells.

The intracellular location of basic fibroblast growth factor (bFGF) was determined in A431 human epidermoid carcinoma cells both on immunofluorescence and on immunoelectron microscopy using a monoclonal anti-bFGF antibody. The immunofluorescence was located in the cytoplasm in quiescent cells. Following the addition of FCS to the culture medium of quiescent sparse cells the growth factor was translocated to and accumulated in the nucleolus.

Differentiation of a human B cell hybridoma with interleukin-2 receptor.

A 6-thioguanine-neo-resistant clone, Raji 6-TGR-neoR was fused with pokeweed mitogen-stimulated B cells (PWM-B cells) obtained from a normal healthy donor, and selected by a new selection medium (HAT-neo). Two hybrid clones, HYNI-1 and HYNI-2, were established. These clones expressed surface IgG and HLA class II antigens derived from PWM-B cells, and they had about 20 more chromosomes than Raji 6-TGR-neoR.

Growth stimulation of adult rat hepatocytes in a primary culture by soluble factor(s) secreted from nonparenchymal liver cell.

Cocultures of adult rat hepatocytes with nonparenchymal liver cells (NPC) resulted in stimulation of DNA synthesis of hepatocytes and their survival for more than one month. These cells were found capable of synthesizing albumin and 3-methylcholanthrene-inducing cytochrome P-450. A conditioned medium obtained by culturing NPC was effective to the same degree as the coculture with NPC for stimulating DNA synthesis in hepatocytes.

Effect of dimethyl sulfoxide on cytosolic ionized calcium concentration and cytoskeletal organization of hepatocytes in a primary culture.

The addition of dimethyl sulfoxide (DMSO) to a chemically defined, serum free medium prolonged hepatocytes survival in primary culture. DMSO exposure had a remarkable effect on morphological change and F-actin filaments distribution of hepatocytes. When hepatocytes were cultured in a medium containing 2% DMSO, the cells showed a compact and cubical shape and intracellular F-actin filaments were mainly observed in a ring-like fashion around the intercellular space.