Increased production of isobutanol from xylose through metabolic engineering of Saccharomyces cerevisiae overexpressing transcription factor Znf1 and exogenous genes.

Only trace amount of isobutanol is produced by the native Saccharomyces cerevisiae via degradation of amino acids. Despite several attempts using engineered yeast strains expressing exogenous genes, catabolite repression of glucose must be maintained together with high activity of downstream enzymes, involving iron-sulfur assimilation and isobutanol production. Here, we examined novel roles of nonfermentable carbon transcription factor Znf1 in isobutanol production during xylose utilization.

Antimicrobial sesquiterpenes from the cultured mycobiont against methicillin-resistant isolated from Vietnamese street foods.

Traditionally, lichen has been used for many purposes, but there remains a lack of understanding regarding the chemical composition and antimicrobial characteristics of , a lichen native to Vietnam. In this study, four sesquiterpenes, diorygmones B-E (1-4), one phenolic compound, 3,5-dihydroxy-4-methoxybenzoic acid (5), and one sterol, β-sitosterol (6), were isolated and structurally elucidated from the cultured mycobiont of the lichen . Additionally, two compounds, stictic acid (7) and norstictic acid (8), were also isolated from the lichen Compounds 2-4 were new compounds.

Repurposing phone booths into COVID-19 sampling stations: medical operator experiences.

This study investigated the repurposing of phone booths into COVID-19 sampling stations and assessed medical operator experiences in twelve healthcare facilities in Thailand. Amidst the pandemic, these facilities became sampling stations as an emergency measure to enhance medical staff protection. The stations, repurposed from decommissioned phone booths, aimed to reduce COVID-19 infection risks among healthcare workers and patients.

Allosteric Signal within the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein Mediated by a Class 3 Monoclonal Antibody Revealed through Molecular Dynamics Simulations and Protein Residue Networks.

This study investigated the allosteric action within the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein caused by class 3 monoclonal antibody (mAb) binding. As the emergence of SARS-CoV-2 variants has raised concerns about the effectiveness of treatments by antibodies, targeting the highly conserved class 3 epitopes has become an alternative strategy of antibody design. Simulations of explicitly solvated RBD of the BA.

Anticancer and anti-angiogenic activities of mannooligosaccharides extracted from coconut meal on colorectal carcinoma cells .

Colorectal carcinoma (CRC) is one of the most common malignancies, though there are no effective therapeutic regimens at present. This study aimed to investigate the inhibitory effects of mannooligosaccharides extracted from coconut meal (CMOSs) on the proliferation and migration of human colorectal cancer HCT116 cells . The results showed that CMOSs exhibited significant inhibitory activity against HCT116 cell proliferation in a concentration-dependent manner with less cytotoxic effects on the Vero normal cells.

Thermodynamic and Kinetic Equilibrium for Adsorption of Cellulosic Xylose of Commercial Cation-Exchange Resins.

The development of low-cost purification technology is an indispensable need for industrial biorefinery. Xylose is easily obtained from hydrothermal pretreatment of lignocellulosic biomass. This current study emphasizes the chromatographic monosaccharide separation process using commercial cation-exchange resins (CER) including Amberlite 120 and Indion 225 to separate xylose from a mixture of hydrolysates.

New biomarkers underlying acetic acid tolerance in the probiotic yeast Saccharomyces cerevisiae var. boulardii.

Evolutionary engineering experiments, in combination with omics technologies, revealed genetic markers underpinning the molecular mechanisms behind acetic acid stress tolerance in the probiotic yeast Saccharomyces cerevisiae var. boulardii. Here, compared to the ancestral Ent strain, evolved yeast strains could quickly adapt to high acetic acid levels (7 g/L) and displayed a shorter lag phase of growth.

Drug-Releasing Tannic Acid-Mediated Adhesive PEG Hydrogel for Porous Titanium Implants.

Porous titanium implants are commonly utilized for orthopedic surgery because they can mimic the mechanical properties and porous structure of human bone. However, the bioinertness of titanium (Ti) has been reported to obstruct biointegration processes, resulting in slower bone repair. Here, we propose a localized drug delivery system on Ti surfaces using adhesive hydrogel to enhance biological-Ti interactions.

Individual honey bee tracking in a beehive environment using deep learning and Kalman filter.

The honey bee is the most essential pollinator and a key contributor to the natural ecosystem. There are numerous ways for thousands of bees in a hive to communicate with one another. Individual trajectories and social interactions are thus complex behavioral features that can provide valuable information for an ecological study.

An assessment of biaxial flexural strength, degree of monomer conversion, color stability, and ion release in provisional restorations containing Sr-bioactive glass nanoparticles.

This study examined the mechanical and chemical properties of an experimental provisional restoration containing Sr-bioactive glass nanoparticles (Sr-BGNPs) compared to commercial provisional materials. The experimental material (TempS10) contained dimethacrylate monomers with added 10 wt% Sr-BGNPs. The degree of monomer conversion (DC) of self-curing ( = 5), biaxial flexural strength (BFS)/modulus (BFM) ( = 5), and color changes (ΔE*) of materials in red wine ( = 5) were determined.

Consistent patterns of common species across tropical tree communities.

Trees structure the Earth's most biodiverse ecosystem, tropical forests. The vast number of tree species presents a formidable challenge to understanding these forests, including their response to environmental change, as very little is known about most tropical tree species. A focus on the common species may circumvent this challenge.

Exogenous γ-aminobutyric acid and Bacillus pumilus reduce arsenic uptake and toxicity in rice.

In this study, the addition of γ-aminobutyric acid (GABA), Bacillus pumilus, or both, was found to enhance rice growth and yield while significantly decreasing arsenic (As) accumulation in Oryza sativa rice tissues. GABA emerged as a regulator of iron (Fe) homeostasis, acting as a signaling modulator that influenced phytosiderophore secretions in the plant. Meanwhile, B.

Real-time kinetic analysis and detection of glycated hemoglobin A1c using a quartz crystal microbalance-based aptasensor.

Glycated hemoglobin (HbA1c) has been an important biomarker for long-term diagnosis and monitoring of diabetes mellitus. The development of a rapid, reliable, and less sophisticated device to measure HbA1c is imperative to facilitate efficient early-care diabetes management. To date, no existing aptamer-based biosensor (aptasensor) for detecting HbA1c has been developed using a quartz crystal microbalance (QCM).

Utilisation of and factors associated with non-COVID-19 healthcare services in public facilities amongst cross-border migrants in Thailand, 2019-2022.

Background: It is believed that the COVID-19 pandemic might disrupt routine healthcare services. A vulnerable group such as cross-border migrants is of critical concern if the pandemic affects their service utilisation. In this study, we aim to explore the impact of COVID-19 and other related factors on non-COVID-19 service amongst cross-border migrants in Thailand.

Hydrolysis of ionic liquid-treated substrate with an Iocasia fonsfrigidae strain SP3-1 endoglucanase.

Recently, we reported the discovery of a novel endoglucanase of the glycoside hydrolase family 12 (GH12), designated IfCelS12A, from the haloalkaliphilic anaerobic bacterium Iocasia fonsfrigidae strain SP3-1, which was isolated from a hypersaline pond in the Samut Sakhon province of Thailand (ca. 2017). IfCelS12A exhibits high substrate specificity on carboxymethyl cellulose and amorphous cellulose but low substrate specificity on b-1,3;1,4-glucan.

Factors affecting the reduction rate of odontogenic cysts after decompression based on 3-dimensional volumetric analysis.

Purpose: This study aimed to investigate the potential factors that could affect the reduction rate of odontogenic cysts following decompression using cone-beam computed tomography (CBCT) for 3-dimensional volumetric analysis.

Materials And Methods: The study sample consisted of CBCT images of 41 individuals who underwent decompression of odontogenic cysts at the Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Chulalongkorn University, between 2010 and 2022. Preoperative and postoperative CBCT results were collected, and a volumetric analysis was conducted to evaluate the differences in the reduction rate and the percentage of volume reduction of cystic lesions based on different parameters.

Vocal fingerprinting reveals a substantially smaller global population of the Critically Endangered cao vit gibbon (Nomascus nasutus) than previously thought.

The cao vit gibbon (Nomascus nasutus) is one of the rarest primates on Earth and now only survives in a single forest patch of less than 5000 ha on the Vietnam-China border. Accurate monitoring of the last remaining population is critical to inform ongoing conservation interventions and track conservation success over time. However, traditional methods for monitoring gibbons, involving triangulation of groups from their songs, are inherently subjective and likely subject to considerable measurement errors.

Axial stress versus strain responses of CFRP confined concrete containing electronic waste aggregates.

This research work investigates the axial stress versus strain responses of un-strengthened and carbon fiber reinforced polymer (CFRP) composites strengthened concrete specimens made with electronic waste coarse aggregates. For this purpose, 36 circular and non-circular 300 mm high concrete specimens constrained with CFRP sheets and partially replaced with E-waste coarse aggregates were prepared. The effect of cross-sectional geometry, 20% partial substitution of natural coarse aggregates with E-waste aggregates, corner effect of non-circular concrete specimens, confinement of specimens with CFRP sheets, and effect of the number of confinement sheets were also studied.

Energy consumption and carbon emission assessment of battery swapping systems for electric motorcycle.

This work investigated different conceptual models for electric motorcycles, which are electric motorcycles with a home charging system, electric motorcycles using a battery swapping system, and electric motorcycles with battery swapping and photovoltaic systems, in four Southeast Asia countries. The current research focused on analyzing the impact of factors such as the number of battery packs in a swapping station, variation in battery swapping demand, the season, and photovoltaic panel size on energy utilization and carbon emissions associated with the entire energy supply chain. The objective of the current study was to evaluate and compare the well-to-wheel emissions using these different conceptual models, considering the varying energy mixes in four countries with a significant prevalence of motorcycle ownership, Thailand, Vietnam, Malaysia, and Indonesia.

Unveiling the microbial dynamics in vermicomposting with coir pith as earthworm substrate.

This study explored the impact of incorporating coir pith, a byproduct of the coconut industry, into the vermicomposting substrate of earthworms. The groups were compared based on their diets: cow manure only or cow manure mixed with varying amounts of coir pith. The aim was to assess the effects of coir pith on earthworm growth, mortality and the microbial community involved in vermicomposting.

Dual lignin-derived polymeric system for peptone removal from simulated wastewater.

The long-term existence of peptone can breed a large number of bacteria and cause the eutrophication of municipal wastewater. Thus, removing peptone in the wastewater is a major challenge facing the current industry. This study used cationic and anionic lignin polymers, i.

Characteristics of Amorphophallus konjac as indicated by its genome.

Amorphophallus konjac, belonging to the genus Amorphophallus of the Araceae family, is an economically important crop widely used in health products and biomaterials. In the present work, we performed the whole-genome assembly of A. konjac based on the NovaSeq platform sequence data.

Genetic diversity, determinants, and dissemination of lineages implicated in melioidosis in northeast Thailand.

Melioidosis is an often-fatal neglected tropical disease caused by an environmental bacterium . However, our understanding of the disease-causing bacterial lineages, their dissemination, and adaptive mechanisms remains limited. To address this, we conducted a comprehensive genomic analysis of 1,391 isolates collected from nine hospitals in northeast Thailand between 2015 and 2018, and contemporaneous isolates from neighbouring countries, representing the most densely sampled collection to date.

Evaluate the potential use of TonB-dependent receptor protein as a subunit vaccine against Aeromonas veronii infection in Nile tilapia (Oreochromis niloticus).

Aeromonas veronii is an emerging bacterial pathogen that causes serious systemic infections in cultured Nile tilapia (Oreochromis niloticus), leading to massive deaths. Therefore, there is an urgent need to identify effective vaccine candidates to control the spread of this emerging disease. TonB-dependent receptor (Tdr) of A.