[Effect of quercetin on some indicators of the proteinase-proteinase inhibitor system in rats upon administration of cobalt chloride to them].

The results of quercetin effect on some changes of proteinase--proteinase inhibitor system parameters in rats under cobalt chloride injection are shown. It was established that preliminary quercetin administration prevened neutral proteinase activation and alpha-2-macroglobulin activity decreasing after 2 h of cobalt chloride influence.

[Effect of actinomycin D and cycloheximide on lipoproteins in blood serum and liver cytosol of rats under oxidative stress].

The influence of protein synthesis inhibitors on rat lipoprotein content and composition under oxidative stress caused by cobalt chloride injection has been investigated in the present work. It has been concluded that apoprotein synthesis is very important process influencing on adaptive reactions under free-radical oxidation activation conditions. Co-administration of cobalt chloride and actinomycin D or cycloheximide (the inhibitors of the protein synthesis) has realy prevented hyperlipoproteinemia in many cases but hasn't influenced on lipoprotein oxidation.

[Effect of pentoxyphylline on certain indicators of the proteinase-proteinase inhibitor system in rats upon administration of cycloheximide].

The pentoxifylline influence on neutral proteinase, alpha-2-macroglobulin, trypsin-alpha-1-proteinase inhibitor and elastaseinhibitory activity under cycloheximide injection has been investigated. Two hours after cycloheximide injection the activity of neutral proteinases increases in rats serum, lungs, heart, liver and kidneys. The preliminary injection of pentoxifylline prevents increase of neutral proteinases activity.

Theory of beam-plasma instability in a periodic plasma-filled waveguide.

The beam-plasma wave interaction in a periodic plasma-filled waveguide is treated in a mathematically correct manner on the basis of the integral equation (IE) method. It has been shown that the relevant boundary-value problem could be reduced to an IE with a singular kernel for the longitudinal component of the electric field on the waveguide axis. The regularization of the IE was performed by extracting the static part of the kernel.

[Antioxidative homeostasis of critically ill individuals].

Content of primary and secondary products of lipid peroxidation; fat soluble vitamins: A, E, carotene, tocopherol's metabolites and total antioxidative activity were investigated in experiments with serum blood of donors and sick people with the acute surgical trauma. It was showed that during the critical states of sick people there is watched the strain of adaptive systems of the whole organism with the resource mobilisation of fat soluble vitamins and appearance of signs of their exhaustion, modification of the direction of metabolism of the unsaturated fat acids from certain formation of eicosanoids to the avalanche looking lipid peroxidation with the accumulation of poisonous products in blood. Also there intensify the formation of active tocopherol's metabolites, what is necessary for the re-establishment of the violative balance in the system of antioxidative protection.

[Exposure to ionizing radiation decreases hemoglobin binding to erythrocyte membrane].

The influence of gamma-radiation at doses 10, 10(2) and 10(3) Gy on binding of hemoglobin to the erythrocyte membrane by inductive--resonance energy transfer in the donor-acceptor pair anthracene-hemoglobin was studied. It was found that binding of hemoglobin to the erythrocyte membrane decreases with increasing dose of gamma-radiation.

[The characteristics of the effect of thyroxine on sphingosine and phosphoinositide synthesis in the rat liver in ontogeny].

The age peculiarities of sphingosine, phosphatidylinoside, phosphatidylinoside 4-phosphate and phosphatidylinoside 4,5-bisphosphate in liver and thyroxine influences on this processes has been investigated. It has been determined that sphingosine, phosphatidylinoside and phosphatidylinoside 4-phosphate synthesis in liver drop in old age. It was found that thyroxine enhances the labelled precursor incorporation into sphingosine of 3- and 24-old rat, and phosphatidylinoside 4,5-bisphosphate--24-old animals and decreases the synthesis of phosphatidylinoside and phosphatidylinoside 4-phosphate in liver of the young rats.

Surface waves in strongly irradiated dusty plasmas.

High-frequency surface waves at the interface between two dusty plasmas subject to radiation are considered. Ultraviolet radiation with energy flux larger than the photoelectric work function of the dust surface causes photoemission of electrons. The dust charge and the overall charge balance of the plasma are thus modified.

[Proteinase-proteinase inhibitor complex in rats under oxidative stress caused by administration of cobalt chloride].

Mechanisms of proteinase-inhibitor proteinase system response was estimated following of cobalt chloride injection. The increase proteinase activity, which led to significant decrease of alpha-2-macroglobulin (alpha-2-MG) level was established that indicated to the removal of the proteinase in complex with alpha-2-MG from the organism. Increase of alpha-1-proteinase inhibitor (alpha-1-PI) trypsin-inhibitory activity in the kidneys testify about removal of oxidative alpha-1-PI.

High-Resolution Infrared Study of the nu(7), nu(8), and nu(15) Bands and Millimeter-Wave Investigation of the v(8) = 1 State of CF(2)Cl-CH(3).

High-resolution (Deltavarsigma = 2.3 and 2.9 x 10(-3) cm(-1)) FTIR spectra of natural and (35)Cl monoisotopic CH(3)CF(2)Cl have been recorded at -70 degrees C in the 600-1400 cm(-1) range.

[Structure-functional states of liver mitochondrial membranes of rats exposed to irradiation].

The effect of the low dose gamma-irradiation (270 cGy--one-fold; 90 cGy per day during 3 days) on oxidative phosphorylation, lipid peroxidation, microviscosity of the annular and free lipids membrane, and membrane protein structural state was studied. The post-radiation influence on membrane functional activity and structural state in accordance with the irradiation regimes was established.

Conformational transitions of DNA induced by changing water content of the sample: a theoretical study.

A semi-phenomenological model with spatially distributed parameters is suggested to describe the processes of conformational transitions induced with change of water content of wet DNA samples. It allows describing conformational dynamics of DNA molecules with heterogeneous primary structures. It has been shown that the process of cooperative conformational transition can be simulated as propagating front of a new conformation.

[The effect of ionizing radiation on Ca ATPase of erythrocyte membranes].

Erythrocyte ghosts were irradiated with doses of 4 x 10(-3)-10(3) Gy. The activity of Ca(2+)-ATPase, the kinetic characteristics of enzyme reaction, the Hill's coefficient, the association constant and a number of La3+ linking centers were determined. The structural--functional peculiarities of Ca(2+)-ATPase changes under exposure to 4 x 10(-3) and 40 Gy of ionizing radiation have been established.

[Study of model protein-lipid systems by the energy transfer method].

Complexes of ribonuclease, lysozyme, cytochrome c and hemoglobin with model phospholipid membranes composed of phosphatidylcholine and diphosphatidylglycerol (4:3, mol:mol) were investigated by the method of non-radiative fluorescence energy transfer. Evidence for the penetration of proteins in to the lipid bilayer interior was obtained. The size of the protein fragment incorporated into the polar membrane region was estimated.

Millimeter-Wave Spectrum of CF(2)Cl(2), Taking into Account the Hyperfine Structure.

A new investigation of the CF(2)(35)Cl(2) ground state rotational spectrum in the 50-100 GHz frequency range gave an opportunity to improve the accuracy of rotational and quartic distortion parameters by an order of magnitude and to determine values of six sextic centrifugal distortion parameters. A special technique was used to process line profiles of unresolved or partially resolved hyperfine structures of rotational transitions. It allowed us to improve significantly values for the diagonal parameters of quadrupole coupling chi(aa), (chi(bb)-chi(cc)) and to determine the off-diagonal parameter chi(ab).

[Changes in the model membrane structure induced by ribonuclease and lysozyme studied by the fluorescent probe technique].

Using fluorescent probes DSM and DSP-12, the effect of ribonuclease and lysozyme on the structural state of liposomes composed of phosphatidylcholine and diphosphatidylglycerol was studied. A correlation between the changes in probe quantum yield and the amount of protein-bound lipids was established. It is assumed that the formation of protein-lipid complexes increases the packing density of lipids and restricts their mobility.

The Coriolis-Coupled States v(6) = 1 and v(8) = 1 of DCOOH.

The Fourier transform gas-phase infrared spectra of the Coriolis-perturbed nu(6) and nu(8) bands of deuterated formic acid DCOOH were measured with a resolution of ca. 0.003 cm(-1).

[Effects of natural zeolite-clinoptilollite on processes of removal of Cs-137 from the rat body].

The selectivity of natural and synthetic zeolites to 137Cs in experiments in vitro has been investigated. The influence of natural zeolite-clinoptilolite on the dynamics of withdrawal of 137Cs from rats' organism was estimated.

Resonance energy transfer study of hemoglobin complexes with model phospholipid membranes.

By examining the resonance energy transfer between fluorescent probes, embedded in the lipid bilayer (4-(dimethylaminostyryl)-1-methylpiridine, 4-(dimethylaminostyryl)-1-dodecylpiridine, N,N'-bishexamethylenrhodamine, rhodamine 6G) as donors, and the heme group of hemoglobin as acceptor, the structure of the protein complexes with the model membranes composed of phosphatidylcholine and cardiolipin was characterized. Quantitative interpretation of the experimental data was performed in terms of the model of energy transfer in two-dimensional systems, using a set of parameters including the distance of closest approach between donor and acceptor, the vertical separation of donor planes, the acceptor distance from the donor plane and the orientation factor. The limits for the heme distance from the lipid bilayer center and the depth of the protein penetration in the membrane interior were estimated.

He-Ne laser light induced changes in the state of chromatin in human cells.

Cells of human buccal epithelium were irradiated in vitro by laser light at a wavelength of 632.8 nm and and an intensity of 1 mW/cm(2). The irradiation induced changes in the chromatin condensation state.

Role of thyroid hormones in regulation of sphingomyelin metabolism in the liver.

Inhibition of thyroid gland function in rats with mercasolil sharply decreased thyroxine and triiodothyronine levels in blood serum and increased acid sphingomyelinase activity and sphingomyelin content in liver. Thyroxine injected into hypothyroid rats normalized the sphingomyelin content, reduced the free ceramide content, and further increased the acid sphingomyelinase activity in liver. Thyroxine stimulated de novo sphingomyelin synthesis.

Structure of cytochrome c complexes with phospholipids as revealed by resonance energy transfer.

Resonance energy transfer between a series of lipid-bound fluorescent probes as donors and the heme group of cytochrome c as acceptor has been used to obtain structural information on the protein complexes with model membranes, composed of phosphatidylcholine and cardiolipin. Analysis of experimental data in terms of the model of energy transfer in two-dimensional systems provides further evidence for preferential cytochrome c orientation with respect to the lipid bilayer and penetration of the protein into the membrane interior.

[The physical mechanism of the effect of low-intensity electromagnetic radiation on biological cells].

The presence of dissolved air and air in the free form (bubbles) was taken into account in the study of electromagnetic radiation on biological cells. It is shown that, upon movement in a temperature gradient field, some bubbles increase in size due to the dissolving of others and the coalescence of bubbles during collisions. As a result, the concentration of dissolved air decreases, which leads to cell responses.

[Fluorescent energy transfer study of lysozyme complexes with liposomes].

The method of radiationless energy transfer was used to study the structure of lysozyme complexes with liposomes composed of phosphatidylcholine and diphosphatidylglycerol (4:3, mol:mol). 4-(n-Dimethylaminostyryl)-1-methylpyridinium n-toluenesulfonate, 4-(n-dimethylaminostyryl)-1-hexylpyridinium n-toluenesulfonate, 4-(n-dimethylaminostyryl)-1-dodecylpyridinium n-toluenesulfonate, and 3-metoxybenzanthrone were used as donors, and nile blue and rhodamine 6G, as acceptors. An increase in the surface area of model membranes upon binging of the protein to lipid bilayer was found.