Changes in the Expression of Immune Proteasomes in the Liver after the Induction of Portal Tolerance Depending on Donor-Recipient Differences in Rats.

Induction of donor-specific tolerance in outbred Wistar rats (RT1) and inbred August rats (RT1) increased the expression of immune proteasome subunits in liver with a peak on day 7 after beginning of the induction. The increase in the level of immune subunits LMP2 and LMP7 was more pronounced in the liver of August rats in comparison with Wistar rats (by 2 and 6 times, respectively), which was associated with higher concentrations of monoamines in the CNS of August rats. After induction of donor-specific tolerance in August and Wistar rats, the immune subunits were in cells of sinusoidal lining and in cells located in sinusoid lumens.

[The classification of the injuries inflicted to the human body by gunshots from the pneumatic weapons].

Unlabelled: The classification of the injuries inflicted to the human body by gunshots from the pneumatic weapons remains to be developed. The objective of the present work was to elaborate the classification of the injuries caused by gunshots from the pneumatic weapons based on the analysis of 98 expert

Conclusion: and acts of forensic medical expertises (surveys) of living subjects (n=76) and corpses (n=22) affected by gunshots from the pneumatic weapons. These materials were collected from the bureaus of forensic medical expertise in different regions of the Ukraine during the period from 2006 till 2015.

[State of immune and cytokine status in acute ulcerative gastroduodenal bleeding].

The paper analyzes the results of a survey of 37 patients with acute ulcerative gastroduodenal hemorrhage. During endoscopy found that 7 patients the cause of bleeding was gastric ulcer, duodenal ulcer in 27 and 3 concomitant ulcer. Hemostasis was evaluated by J.

[Pregnancy and experimental autoimmune encephalomyelitis induced in different terms of gestation period].

Objective. To investigate the features of the clinical course of experimental demyelinating pathology as a model of multiple sclerosis in rats in different trimesters of pregnancy. Material and methods.

Adhesion, growth, and proliferation of endothelial cells on biopolymer extracellular film matrices.

We analyzed the influence of initial properties of biopolymer fi lm extracellular matrices made of chitosan on adhesion of swine embryonic kidney epithelium-like cells, their morphology, growth, and proliferation. It was found that adhesion index of cells on the studied matrices was similar, but cell morphology had significant qualitative differences. Round non-flattened cells were described in endothelial cell cultures on chitosan-based matrices.

Content of IL-10 and CD4+CD210+ cells in mice with adjuvant arthritis before and after treatment with cryopreserved placental cells.

We analyzed the serum level of IL-10, blood content of CD4+СD210+ cells, and expression of IL-10 receptors in adjuvant arthritis before and after treatment with native and cryopreserved placental cells. In animals with experimental adjuvant arthritis, a negative correlation was found between the index of arthritis and IL-10 concentration (r=-0.7; p=0.

Methyl N-(3-cyano-picolino-yl)-l-tryptophanate.

In the title compound, C19H16N4O3, the stereocenter has an l configuration; l-tryptophan methyl ester hydro-chloride being used as a starting material. The indole ring system and the pyridine ring are inclined to one another by 13.55 (14)°.

[Genetic diversity and relationships of northern eurasia populations for polymorphic Alu-insertions].

We for the first time have examined the autosomal gene pool of the Siberia, Central Asian and the Far East populations (27 populations of 12 ethnic groups) using a set of polymorphic Alu insertions in the human genome. The results of the analysis testify (i) to a significant level of genetic diversity in the Northern Eurasian populations and (ii) to a considerable differentiation of gene pool in the population of this region. It has been shown that at the CD4 locus, the frequency of Alu (-) is inversely related to the Mongoloid component of the population, the lowest and highest frequencies of the Alu deletion at locus CD4 were recorded respectively in Eskimo (0.

The use of nanocomposite coatings with various physicochemical properties in tissue engineering.

We studied the effect of nanocomposite coatings with various physicochemical properties on the structural and functional properties (adhesion potential, phenotype, gene expression) of mesenchymal stem cells. Of all tested nanocoatings (Al2O3, ZrO2, Ta2O5), oxide coating Al2O3 enriched in vitro monolayer bone marrow cell culture with cells carrying mesenchymal stem cells phenotype markers and stimulated expression of ido gene, which can confer new therapeutic potencies to these cells and extend their application in clinical practice.

[Socio-economic and health organizational problems of emergency and out-patient care for elderly and senile patients with arterial hypertension (according to the sociological monitoring)].

The article contains data about the peculiarities of socio-economic and health-organization factors in the rendering emergency and out-patient care for elderly and senile patients with arterial hypertension. The results of study of satisfaction by medical care quality depending on the age and gender of the patients are discussed. A large number of living alone and widowed patients among elderly patients is marked.

The use of catalytic carbon deposits as 3D carriers for human bone marrow stromal cells.

We studied the possibility of using 3D structures based on carbon catalytic deposits as carriers for human bone marrow stromal cells. It was found that carbon catalytic deposits obtained by gas deposition method using FeCl(3) × 6H(2)O as the catalyst are a biocompatible material for human bone marrow stromal cells promoting adhesion, proliferation, and distribution of cells within the 3D carrier, and therefore can be used for tissue engineering.

Comparison of the methods for seeding human bone marrow mesenchymal stem cells to macroporous alginate cryogel carriers.

We performed a comparative study of the localization, distribution, metabolic activity, and surface properties of human bone marrow mesenchymal stromal cells after static and perfusion seeding to macroporous alginate cryogels. A simple perfusion system for mesenchymal stromal cell seeding to macroporous alginate cryogel sponges proposed in this study resulted in rapid and uniform distribution of cells within the whole volume of the scaffold preserving functional and morphological properties of the cells.

[Characteristics of antihypertensive therapy in patients of older age groups].

The review presents the data on the characteristics of drug therapy in elderly and senile patients with arterial hypertension in terms of real-life clinical practice. The age and gender differences of these problems are discussed. The pharmacy epidemiologic analysis of antihypertensive therapy shows that nowadays treatment corresponds not fully to modern national and international recommendations about diagnostics and treatment of arterial hypertension.

[Genetic diversity of Khakassian gene pool: subethnic differensiation and the structure of Y-chromosome haplogroups,].

The structure of Khakass gene pool has been investigated: compositions and frequencies of Y-chromosome haplogroups were described in seven population samples of two basic subethnic groups--Sagays and Kachins from three territorially distanced regions of Khakassia Republic. Eight haplogroups: C3, E, N*, N1b, N1c, R1a1a and R1b1b1 have been determined in Khakass gene pool. Significant differences between Sagays and Kachins were shown in haplogroup spectra and a level of genetic diversity in haplogroups and YSTR-haplotypes.

[Skewed X-chromosome inactivation in human embryos with mosaic trisomy 16].

The sex ratio and X-chromosome inactivation were analyzed in placental tissues of human spontaneous abortuses with pure and mosaic forms of chromosome 16 trisomy. The sex ratio value was found to decrease with an increase in the share of cells with the trisomic karyotype, which suggests differential survival of embryos belonging to different sexes. The pattern of X-chromosome inactivation in cells of extraembryonic mesoderm in the control group of embryos and in spontaneous abortuses with the level of trisomy 16 below 80% corresponded to random X-inactivation, whereas in most embryos with a frequency of trisomy 16 exceeding 80% skewed inactivation was observed.

Characteristics of Populations of the Russian Federation over the Panel of Fifteen Loci Used for DNA Identification and in Forensic Medical Examination.

Seventeen population groups within the Russian Federation were characterized for the first time using a panel of 15 genetic markers that are used for DNA identification and in forensic medical examinations. The degree of polymorphism and population diversity of microsatellite loci within the Power Plex system (Promega) in Russian populations; the distribution of alleles and genotypes within the populations of six cities and 11 ethnic groups of the Russian Federation; the levels of intra- and interpopulation genetic differentiation of population; genetic relations between populations; and the identification and forensic medical characteristics of the system of markers under study were determined. Significant differences were revealed between the Russian populations and the U.

[Genetic diversity of X-chromosome in populations of aboriginal Siberian ethnic groups: linkage disequilibrium structure and haplotype philogeography of ZFX locus].

The structure of gene pool of the Siberian aboriginal population has been described based on the data on polymorphism of ZFX gene located on X-chromosome. In ten populations under study 49 haplotypes have been determined, three of which are presented with high frequency. Comparing the obtained results with the available data from HapMap project unique "African" haplotypes were revealed, which occurred in Yoruba population with the frequency of 3-7% and were not found in other populations.

[Comparative characteristics of the gene pool of Teleuts inferred from Y-chromosomal marker data].

The gene pool structure of Teleuts was examined and Y-chromosomal haplogroups composition and frequencies were determined. In the gene pool of Teleuts, five haplogroups, C3xM77, N3a, R1b*, R1b3, and R1a1, were identified. Evaluation of the genetic differentiation of the samples examined using analysis of molecular variance (AMOVA) with two marker systems (frequencies of haplogroups and Y-chromosomal microsatellite haplotypes) showed that Bachat Teleuts were equally distant from Southern and Northern Altaians.

Growth and adipogenic differentiation of mesenchymal stromal bone marrow cells during culturing in 3D macroporous agarose cryogel sponges.

We studied the possibility of population of macroporous agarose cryogel sponges by mesenchymal stromal bone marrow cells with their subsequent adipogenic differentiation. After 7-day culturing of mesenchymal stromal cells in agarose cryogel, the level of cell proliferation was 35%. After 3-week culturing in a medium inducing adipogenesis we observed accumulation of intracellular neutral lipids positively stained with Oil Red O.

[The origin of Yakuts: analysis of Y-chromosome haplotypes].

Gene pool structure of Sakha Republic (Yakutia) native population has been studied: we defined composition and frequencies of Y-chromosome haplogroups for Yakuts. Six haplogroups: C3 x M77, C3c, N*, N2, N3a and R1a1 have been revealed in Yakut gene pool. A greater part of Y-chromosome in Yakut population belongs to N3a haplogroup (89%).

[Gene pool differences between northern and southern Altaians inferred from the data on Y-chromosomal haplogroups].

Y-chromosomal haplogroups composition and frequencies were analyzed in Northern and Southern Altaians. In the gene pool of Altaians a total of 18 Y-chromosomal haplogroups were identified, including C3xM77, C3c, DxM15, E, F*, J2, I1a, I1b, K*, N*, N2, N3a, O3, P*, Q*, R1*, R1a1, and R1b3. The structured nature of the Altaic gene pool is determined by the presence of the Caucasoid and Mongoloid components, along with the ancient genetic substratum, marked by the corresponding Western and Eastern Eurasian haplogroups.

[Association analysis of gene polymorphism in alcohol metabolizing enzymes with risk for coronary atherosclerosis].

The allele and genotype distribution of two alcohol dehydrogenase genes ADH1B (exon 3 polymorphism A/G (47His)), ADH7 (intron 5 polymorphism G/C) and cytochrome P450 2E1 gene (CYP2E1; 5'-flanking region G/C and intron 6 T/A polymorphisms) were examined in Russian (Tomsk, n = 125) healthy population and in coronary atherosclerosis patients (CA, n = 92). The genotype frequencies followed the Hardy-Weinberg equilibrium and the alleles were in linkage equilibrium or gametic equilibrium in the control sample. Only two CYP2E1 gene polymorphisms were in linkage disequilibrium.

[The method of lifting and fixation of superficial musculo-aponeurotical system in rejuvenation operations on the face].

The method of neck-facial facelifting, consisting of restrited dissection of adiposo-cutaneous flap and fascial sheet of superficial musculo-aponeurotical system (SMAS), its suturing using multiple quilts in a closed loop fashion, lifting of the neck-facial tissues en bloc along vertical vector. The thread is conducted subperiosteal in zygomatic arch region, securing safe fixation of the SMAS flap, permits to achieve harmony and to make the rejuvenation effect more durable.

[Principles of patients selection to esthetic plastic surgical clinic].

The original principles of patients selection for esthetic operations performation are presented. Formalized card of registration of medical information that permit to estimate individual peculiarities of any patient is proposed. Presence of functional and organic disturbances in patients show the need of reviewing of present statement that esthetic interventions are performed in healthy patients.