Seed coat colour and structure are related to the seed dormancy and overwintering ability of crop-to-wild hybrid soybean.

The possible persistence of genetically modified (GM) crop-to-wild hybrid seeds in the soil seed bank is a major concern in risk assessment and is closely related to seed characteristics such as dormancy. In the present study, we generated F hybrids via crosses between GM soybean accessions and wild soybean and evaluated the dormancy, overwintering ability and inheritance of foreign genes in different-coloured hybrid seeds (yellow, green, brown and black). The results revealed that the 5-enolpyruvylshikimate-3-phosphate synthase transgene may have no influence on crop wild hybrid seed dormancy and overwintering ability, and the dormancy of the hybrid seeds was closely related to seed coat colour.

Fitness changes in wild soybean caused by gene flow from genetically modified soybean.

Background: Crop-wild hybridization has generated great concerns since gene flow can be an avenue for transgene escape. However, a rather limited number of studies on risk assessment regarding the dispersion of transgenes from GM soybean to populations of its wild relatives have been previously conducted.

Results: The results of the 3-year experiment demonstrated that hybrids between GM soybeans and wild soybean had lower seed germination and higher seed productivity than GM soybean.

Fitness and Ecological Risk of Hybrid Progenies of Wild and Herbicide-Tolerant Soybeans With Gene.

Exogenous genes of transgenic crops are usually transferred to their wild-type relatives through pollen-mediated gene flow, which may change the ecological fitness and ability to invade wild populations, resulting in the weeding of wild plants and other unpredictable environmental impacts. In this study, the F generation of herbicide-resistant soybeans and wild soybeans was obtained by artificial pollination, F generation seeds were obtained by self-crossing, and the fitness of the parents and their F and F generations were tested. The foreign protein was expressed normally in the hybrid between transgenic and wild soybeans; however, the protein expression was significantly lower than that in transgenic soybeans.

Development of an Event-Specific Droplet Digital PCR Assay for Quantification and Evaluation of the Transgene DNAs in Trace Samples of GM -Knockout Goat.

The prion protein () gene encoding prion protein is considered a prerequisite for the occurrence of scrapie disease, and knockout of the gene in transgenic goat is one effective approach to avoid scrapie. This study aims to establish an event-specific droplet digital polymerase chain reaction (ddPCR) assay to detect and quantify the content of genetically modified (GM) -knockout goat event KoP1. The developed ddPCR assay presents high specificity, sensitivity, accuracy, precision and wide dynamic range.

Evaluation of the effect of transgenic Bt cotton on snails Bradybaena (Acusta) ravida and Bradybaena similaris (Ferussac).

The impact of transgenic crops on non-target organisms is a key aspect of environmental safety assessment to transgenic crops. In the present study, we fed two snail species, Bradybaena (Acusta) ravida (B. ravida) and Bradybaena similaris (Ferussac)(B.

Effects of genetically modified maize expressing Cry1Ab and EPSPS proteins on Japanese quail.

A 49-d feeding study was conducted to evaluate the effects of the genetically modified (GM) maize strain C0030.3.5 on Japanese quails (Coturnix japonica) in terms of body performance and egg quality.

Expression of Bt Protein in Transgenic Bt Cotton Plants and Ecological Fitness of These Plants in Different Habitats.

Fitness is one of the key parameters to evaluate the effects of transgenic plants on the ecological environment. To evaluate the ecological risk of transgenic Bt cotton plants growing in different habitats, we determined the expression of the exogenous Bt gene and the fitness of transgenic and non-transgenic cotton plants in three habitats (farmland, grassland, and shrub). We observed that the expression of Bt protein in the farmland was significantly higher than that in the natural habitat, and when the growth environment was suitable, the Bt protein expression level showed a downward trend with the advancement of the growth.