A New Analytical Method for Quantifying Acid-End-Cap PLGA in Sub-Milligram Quantities.

Characterization of PLGA polymers used in FDA-approved drug products is critical for quality control and qualitative/quantitative (Q1/Q2) evaluation of potential generic formulations. Various techniques have been developed and used to characterize the molecular properties of PLGA polymers, such as molecular weight, molecular composition, and molecular structure. Commonly used techniques include gel permeation chromatography (GPC), nuclear magnetic resonance (NMR), semisolvent methods, and GPC-based intrinsic viscosity measurement.

Do MMPI-3 Validity Scale Findings Generalize to Concurrently Administered Measures? Validation with a Forensic Disability Sample.

Objective: Research has demonstrated that over-reporting and under-reporting, when detected by the MMPI-2/-RF Validity Scales, generalize to responses to other self-report measures. The purpose of this study was to investigate whether the same is true for the Minnesota Multiphasic Personality Inventory-3 (MMPI-3) Validity Scales. We examined the generalizability of over-reporting and under-reporting detected by MMPI-3 Validity Scales to extra-test self-report, performance-based, and performance validity measures.

Potential Harmful Behavior Toward Older Family Members: Applying a Double-ABCX Model to Examine Problematic Family Caregiving.

Grounded in Lin and Giles' communication model of elder abuse and neglect and McCubbin and Patterson's double-ABCX model of family stress, this study examined whether caregiver self-efficacy and perceptions of positive caregiving outcomes predicted caregivers' lowered tendency to engage in potential harmful behaviors (PHB) toward care receiver directly or indirectly through lowered reports of care receivers' problem behaviors and negative coping strategy (i.e., communication neglect coupled with expressed anger).

Lactate promotes H3K18 lactylation in human neuroectoderm differentiation.

In mammals, early embryonic gastrulation process is high energy demanding. Previous studies showed that, unlike endoderm and mesoderm cells, neuroectoderm differentiated from human embryonic stem cells relied on aerobic glycolysis as the major energy metabolic process, which generates lactate as the final product. Here we explored the function of intracellular lactate during neuroectoderm differentiation.

Matrix Architecture and Mechanics Regulate Myofibril Organization, Costamere Assembly, and Contractility in Engineered Myocardial Microtissues.

The mechanical function of the myocardium is defined by cardiomyocyte contractility and the biomechanics of the extracellular matrix (ECM). Understanding this relationship remains an important unmet challenge due to limitations in existing approaches for engineering myocardial tissue. Here, they established arrays of cardiac microtissues with tunable mechanics and architecture by integrating ECM-mimetic synthetic, fiber matrices, and induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs), enabling real-time contractility readouts, in-depth structural assessment, and tissue-specific computational modeling.

A modular platform for bioluminescent RNA tracking.

A complete understanding of RNA biology requires methods for tracking transcripts in vivo. Common strategies rely on fluorogenic probes that are limited in sensitivity, dynamic range, and depth of interrogation, owing to their need for excitation light and tissue autofluorescence. To overcome these challenges, we report a bioluminescent platform for serial imaging of RNAs.

Electroanalytical Quantification of DNA Chirality.

Although chirality is critical for molecular properties and functions, experimental quantification of chirality is lacking. Herein, we performed cyclic voltammetry (CV) under polarized magnetic fields to provide a unified scale to quantify and compare DNA chirality. We observed the largest electron spin polarization in DNA structures with opposite chiral senses, which is consistent with the effect of chiral-induced spin selectivity (CISS).

Patient-reported persistent lymphedema and peripheral neuropathy among long-term breast cancer survivors in the Carolina Breast Cancer Study.

Background: Improved breast cancer treatment has lengthened survival but also has long-term impacts. Lymphedema and peripheral neuropathy are treatment-related sequelae that extend into survivorship. Co-occurrence of these conditions may further impair functional well-being.

Fiber composition, in vitro true digestibility, and fiber degradation of hempseed meal compared to mainstream feedstuffs using equine feces as microbial inoculum within the Daisy Incubator.

Recent changes in legislation have put hempseed meal (Cannabis sativa L.; HSM) under consideration as a potential livestock feedstuff. Digestibility of HSM has been researched in other species, however, there are little data in horses.

Differentiation signals induce APOBEC3A expression via GRHL3 in squamous epithelia and squamous cell carcinoma.

Two APOBEC DNA cytosine deaminase enzymes, APOBEC3A and APOBEC3B, generate somatic mutations in cancer, thereby driving tumour development and drug resistance. Here, we used single-cell RNA sequencing to study APOBEC3A and APOBEC3B expression in healthy and malignant mucosal epithelia, validating key observations with immunohistochemistry, spatial transcriptomics and functional experiments. Whereas APOBEC3B is expressed in keratinocytes entering mitosis, we show that APOBEC3A expression is confined largely to terminally differentiating cells and requires grainyhead-like transcription factor 3 (GRHL3).

Student Urology Conference Increases Participant Knowledge of Urology Match and Confidence in Research.

Objective: To study the impact of a student-specific urology conference for students considering a career in urology. The first symposium specifically for medical students interested in urology was designed to provide an opportunity to present their research, explore urological subspecialties, and network with faculty members, residents, and other students.

Methods: Medical students and faculty at a US medical school organized the inaugural New England Student Urology Symposium (NESUS).

A self-efficacy enhancement alcohol reduction intervention for men on-remand in prison: the APPRAISE feasibility pilot RCT.

Background: As many as 70% of remand prisoners have admitted to being under the influence of alcohol when committing the crime leading to their imprisonment. Providing support and advice regarding alcohol consumption can be effective in some groups of people. There is little evidence regarding this for men on remand in prison.

Insomnia Severity and Daytime Sleepiness in Caregivers of Advanced Age.

Objective: Aging-related changes and caregiver burden may increase the risk for sleep disturbances among older caregivers, yet few studies have examined the prevalence of insomnia and daytime sleepiness in this group. We examined the relationship of caregiver status with insomnia and daytime sleepiness among persons of advanced age (>75 years of age).

Design: Cross-sectional.

Picky eaters: Selective microbial diet of avian ectosymbionts.

Individual organisms can function as ecosystems inhabited by symbionts. Symbionts may interact with each other in ways that subsequently influence their hosts positively or negatively, although the details of how these interactions operate collectively are usually not well understood. Vane-dwelling feather mites are common ectosymbionts of birds and are proposed to confer benefits to hosts by consuming feather-degrading microbes.

Young Black and White adults prefer in-person to telehealth for primary care visits and group health promotion programs.

Telehealth utilization has increased since the coronavirus disease 2019 pandemic, reducing barriers to healthcare and, potentially, reducing participation in group health-promotion interventions. However, preferences for telehealth versus in-person formats have not been established. To examine preferences for telehealth and in-person format for primary care and group health-promotion interventions among Black and White women and men aged 20-39.

Association between sleep quality, sleep duration, and physical frailty among adults aged 50 years and older in India.

Background: Considering the significant growth in India's aging population, it is imperative to identify factors associated with frailty among older Indians. This study examined the association between sleep quality, sleep duration, and physical frailty among older adults in India. Moreover, we examined whether the associations between sleep quality, sleep duration, and physical frailty varied by gender.

Dual-sided transparent display enabled by polymer stabilized liquid crystals for augmented reality.

In the past decade, display technology has been reimagined to meet the needs of the virtual world. By mapping information onto a scene through a transparent display, users can simultaneously visualize both the real world and layers of virtual elements. However, advances in augmented reality technology have primarily focused on wearable gear or personal devices.

Toward a Trauma-Informed Comprehensive School Physical Activity Program: Specifying Feasible Practices Across the School Day.

Background: Many students would benefit from trauma-informed physical activity (PA); however, there is a lack of systematic guidance on incorporating trauma-informed practices across school-based PA opportunities. The purpose of this study was to generate a feasible framework for trauma-informed school-based PA.

Methods: Framework development was guided by a modified Delphi approach, including an exploration phase and an evaluation phase.

Analysis of Ohio Nurses' Voting Behaviors 2020-2023.

This study provides baseline data for voting patterns and the political affiliation of licensed nurses (licensed practical nurses [LPNs], registered nurses [RNs], and advanced practice registered nurses [APRNs]) in Ohio for the November 2020, 2022, and 2023 general elections. Using two public databases (licensed nurses and registered voters), the findings for 73.7% of all licensed nurses registered to vote are reported, including 18,894 APRNs, 30,731 LPNs, and 137,353 RNs.

Isotopic heterogeneity in U.S. Urban water supply systems reflects climatic, environmental, and sociodemographic factors: Implications for forensic identification.

The forensic application of stable oxygen isotope data from human tissues depends on naturally occurring isotopic variation in drinking water across geographic areas. One factor which complicates interpretation of forensic data is local variability: if a wide range of drinking water values is in a small geographic region it may be difficult to identify or rule out that region as a location of origin. We examine data from community collection programs documenting tap water isotope variation within 30 cities\developed areas throughout the United States.

Non-Hermitian Skin Effect in Many-Body Thermophotonics.

The tight-binding model, foundational in depicting electronic behaviors in solid-state physics, has recently contributed to the understanding of non-Hermitian skin effects in optics, acoustics, and mechanics. However, tight-binding model is primarily built upon scalar nearest couplings, which in turn does not fit to describe the vectorial long-range interactions inherently in thermophotonics. Here, we report a strategy involving many-body radiative interactions in a two-dimensional thermophotonic lattice, and further reveal two types of orthogonal non-Hermitian skin modes in a reciprocal system.

Multimodality Treatment for Locally Advanced Gastric Adenocarcinoma.

Gastric cancer is prevalent worldwide and is a leading cause of cancer-related death. Patients with GC often present at advanced stages at diagnosis. Patients with locally advanced diseases experience poor survival rates with surgery alone.

Respiratory Outcomes of Infants Born Extremely Preterm in the Necrotizing Enterocolitis Surgery Trial.

The multicenter Necrotizing Enterocolitis Surgery Trial compared initial peritoneal drainage with laparotomy among infants with extremely low birth weight and surgical necrotizing enterocolitis or intestinal perforation. In this post hoc analysis of trial data, initial drainage was associated with adverse respiratory outcomes, both in hospital and through 2 years corrected age.

DNA Catalysis: Design, Function, and Optimization.

Catalytic DNA has gained significant attention in recent decades as a highly efficient and tunable catalyst, thanks to its flexible structures, exceptional specificity, and ease of optimization. Despite being composed of just four monomers, DNA's complex conformational intricacies enable a wide range of nuanced functions, including scaffolding, electrocatalysis, enantioselectivity, and mechano-electro spin coupling. DNA catalysts, ranging from traditional DNAzymes to innovative DNAzyme hybrids, highlight the remarkable potential of DNA in catalysis.