Relevance of Bioassay of Biologically Active Substances (BAS) with Geroprotective Properties in the Model of the Nematode Caenorhabditis Elegans in In Vivo Experiments.

Aging is an inevitable process of nature. The age of living organisms contributes to the appearance of chronic diseases, which not only reduce the quality of life but also significantly damage it. Modern medicines can successfully fight multiple diseases and prolong life.

Experimental Studies of the Effect of Extract on the State of Adaptive Capabilities of Rats under Chronic and General Exposure to Cold.

Currently, there is an objective need to create fortified food products that allow not only to provide the body with energy, but also to replenish the deficiency of essential nutrients. A generalization of the information published by Rospotrebnadzor and the Institute of Nutrition of the Russian Academy of Medical Sciences indicates a deficiency in the diet of Russians of vitamins C, group B and β-carotene and minerals, including calcium and iron, regardless of the season of the year. The identified deviations lead to a violation of the immune status, a decrease in the body's resistance to infections, and other unfavorable environmental factors, leading to an increase in the level of morbidity and a decrease in working capacity.

Calciprotein Particles Link Disturbed Mineral Homeostasis with Cardiovascular Disease by Causing Endothelial Dysfunction and Vascular Inflammation.

An association between high serum calcium/phosphate and cardiovascular events or death is well-established. However, a mechanistic explanation of this correlation is lacking. Here, we examined the role of calciprotein particles (CPPs), nanoscale bodies forming in the human blood upon its supersaturation with calcium and phosphate, in cardiovascular disease.

The type specimens of bees (Hymenoptera, Apoidea) deposited in the Zoological Institute of the Russian Academy of Sciences, St. Petersburg. Contribution IV. Family Andrenidae, genus Andrena Fabricius, 1775, species described by F. Morawitz.

The type specimens for names of bee taxa in the genus Andrena Fabricius, 1775 of family Andrenidae described by F. Morawitz and deposited in the Zoological Institute, Russian Academy of Sciences (St. Petersburg) are reviewed.

Characterization of Ferromagnetic Composite Implants for Tumor Bed Hyperthermia.

Hyperthermia therapy (HT) is becoming a well-recognized method for the treatment of cancer when combined with radiation or chemotherapy. There are many ways to heat a tumor and the optimum approach depends on the treatment site. This study investigates a composite ferromagnetic surgical implant inserted in a tumor bed for the delivery of local HT.

[Pre-clinical studies of cold exposure to evaluate the efficiency and functionality of a specialized product].

Strengthening the adaptive capabilities of the human body during cold exposure is one of the conceptual elements of solving the problem of the well-being of the population of the northern regions of Russia. of the work was to determine the dynamic performance, endurance and to study the morphological parameters of the rat tracheal mucosa against the background of the consumption of functional confectionery products containing the juice of Chinese lemongrass berries (Schisandra chinensis) and dry extract of eleutherococcus (Eleutherococcus) in regular exposure to cold. .

Study of an EuBO-ScBO system and EuSc(BO), EuSc(BO) orthoborates.

The EuBO-ScBO system was investigated by solid state synthesis and DSC methods. In this system, a new EuSc(BO) compound was found. It crystallizes in the 3̄ space group with unit cell parameters of = 4.

Evaluation of Biocompatibility and Antagonistic Properties of Microorganisms Isolated from Natural Sources for Obtaining Biofertilizers Using Microalgae Hydrolysate.

Determination of the biocompatibility of microorganisms isolated from natural sources (Kemerovo Oblast-Kuzbass) resulted in the creation of three microbial consortia based on the isolated strains: consortium I (, , and ), consortium II (, , and ), and consortium III (, ; ; and ). The nutrient media composition for the cultivation of each of the three studied microbial consortia, providing the maximum increase in biomass, was selected: consortium I, nutrient medium 11; consortium II, nutrient medium 13; for consortium III, nutrient medium 16. Consortia I and II microorganisms were cultured at 5-25 °C, and consortium III at 50-70 °C.

Antimicrobial Potential of Microorganisms Isolated from the Bottom Sediments of Lake Baikal.

Extremophilic microorganisms attract researchers by their unique characteristics, primarily antagonistic ones, acquired in the process of survival in extreme natural conditions. The antimicrobial potential of the metabolites of these microorganisms is quite broad, from the food industry to therapeutic drugs. Microbial mats of Lake Baikal are a source of unique and diverse microorganisms.

First Insight into the Diversity and Antibacterial Potential of Psychrophilic and Psychotrophic Microbial Communities of Abandoned Amber Quarry.

Natural habitats, including extreme ones, are potential sources of new antimicrobial compound producers, such as bacteriocins and enzymes, capable of degrading the matrix polysaccharides of bacterial biofilms. This study aimed to investigate biodiversity and evaluate the antibacterial potential of psychrophilic and psychrotrophic microbial communities of the flooded Walter amber quarry (Kaliningrad region, Russia). As a result of 16S rDNA high-throughput profiling, 127 genera of bacteria belonging to 12 phyla of bacteria were found in sediment samples: sp.

Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content.

Our study focused on investigating the possibilities of controlling the accumulation of carbohydrates in certain microalgae species ( Gomont, Beijer, and Dunaliella Teod) to determine their potential in biofuel production (biohydrogen). It was found that after the introduction of carbohydrates (0.05 g⋅L) into the nutrient medium, the growth rate of the microalgae biomass increased, and the accumulation of carbohydrates reached 41.

A Study of the Antioxidant, Cytotoxic Activity and Adsorption Properties of Karelian Shungite by Physicochemical Methods.

This study reveals that fossil shungite samples exhibit antioxidant activity, can reduce oxidized components, and bind to free radicals. A sample of Sh20 (size fraction-20 μm) (1.30 mg equivalents of ascorbic acid/g of shungite; 3.

Study of the Properties of In Vitro (L.) Soó (Family ) Extracts.

The medicinal plant (L.) Soó (family ) is used to treat gastritis, colic, gastrointestinal tract, and bladder diseases. This study aimed to investigate the properties and characteristics of the in vitro extract.

Study of the Biologically Active Properties of Medicinal Plant .

The results of the studies have shown that to obtain an extract of a complex of biologically active substances of , ethyl alcohol (mass fraction of alcohol 70%) with a hydromodule of 1:5 should be used, and the extraction should be carried out for 60 min at a temperature of 60 °C. The investigated plant extracts with the complex of bioactive substances from the leaves and flowers are safe from the point of view of the content of heavy metals, pesticides, aflatoxin B1, radionuclides, as well as pathogenic and opportunistic microorganisms. It has been established that the extract contains rutin, hyperoside, ferulic acid, quercetin, kaempferol, disulphuretin, sulphurein, sulphurein, gallic acid, methyl gallate, pentagalloyl glucose, 3,3',4',5,6,7-hexahydroxyflavonone, 3,3',4',5,5',7-hexahydroxyflavonone, 3--α-L-rhamnofuranoside, 3,3',4',5,5',7-hexahydroxyflavulium(1+), 7--β-D glucopyranoside, and 3,3',4',7-tetrahydroxyflavonone.

Pathomorphological Changes in the Brain in Dynamics of Long-Term Dust Exposure.

Histological and morphometric studies of brain autopsy material showed that the development of hypoxic changes in miners starts at the early stages of working in the dusty atmosphere. Edema of the pericellular and perivascular zones and the pia mater, degenerative changes in some nerve cells and even their loss and formation of gliosis foci were identified. The revealed changes in neurons progressed with increasing the duration of working under hazardous conditions.

First-Principle Studies of the Vibrational Properties of Carbonates under Pressure.

Using the density functional theory with the hybrid functional B3LYP and the basis of localized orbitals of the CRYSTAL17 program code, the dependences of the wavenumbers of normal long-wave vibrations on the (GPa) pressure (cm) = + (/)· + (/)· and structural parameters (Å) (: , , , , ): (cm) = + (/) - ( - ) were calculated. Calculations were made for crystals with the structure of calcite (MgCO, ZnCO, CdCO), dolomite (CaMg(CO), CdMg(CO), CaZn(CO)) and aragonite (SrCO, BaCO, PbCO). A comparison with the experimental data showed that the derivatives can be used to determine the pressures, , , lattice constants and the metal-oxygen, and the carbon-oxygen interatomic distances from the known Δ shifts.

Production, Purification, and Study of the Amino Acid Composition of Microalgae Proteins.

Microalgae are known to be rich in protein. In this study, we aim to investigate methods of producing and purifying proteins of 98 microalgae including , sp., , and (Baltic Sea).

Evaluation of the Conditions for the Cultivation of Callus Cultures of Regarding the Yield of Polyphenolic Compounds.

The cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of were chosen for study.

[Comparative evaluation of results of laboratory researches of milk in Kuzbass while carrying out control and surveillance measures for identifying false].

Milk is one of the main products of the consumer basket, a source of nutrients, and has specific characteristics due to its chemical and microbiological composition. Falsification of dairy products against the background of an increase in production cost dictates the need for quality control and sanitary and epidemiological supervision in the framework of protecting consumer rights and human well-being. of the work is a comparative assessment of the results of laboratory studies of milk sold in retail of the Kemerovo Region (Kuzbass) during detection of falsification.

Short/long-term cryopreservation prior to comet assay of whole-blood leukocytes and -cultured lung fibroblasts.

Single-cell gel electrophoresis (comet assay) is a valuable test that can be used in ecotoxicological, epidemiological, and biomonitoring contexts. We assessed the effects of short- (without cryopreservation) and long-term (with cryopreservation) storage of DMEM-cultivated human peripheral blood leukocytes (HPBLs) and a human lung fibroblast cell line (FLECH-104) on comet assay results. Samples were stored for 6 or 24 h at room temperature (23°С) or 4 °C and frozen at -80 °C or -196 °C for 1, 2, or 4 weeks.

Chondroprotection and Molecular Mechanism of Action of Phytonutraceuticals on Osteoarthritis.

Osteoarthritis (OA) is a degenerative joint disease and an important cause of incapacitation. There is a lack of drugs and effective treatments that stop or slow the OA progression. Modern pharmacological treatments, such as analgesics, have analgesic effects but do not affect the course of OA.

Immuno-hormonal network in postmenopausal women: disturbance in breast cancer patients.

Introduction: Antibodies to estradiol and progesterone (Es and Pg) modulated their blood serum concentration and biological effects in immunized animals. Antibodies to membrane steroid receptors acted as hormone agonists or antagonists in cell cultures.

Material And Methods: Here we studied the levels of Es and Pg, idiotypic immunoglobulin (Ig) A1 and anti-idiotypic IgG2 specific to Es and Pg in the serum of postmenopausal women (82 healthy donors and 443 breast cancer patients).

Study of the l-Phenylalanine Ammonia-Lyase Penetration Kinetics and the Efficacy of Phenylalanine Catabolism Correction Using In Vitro Model Systems.

The kinetics of l-phenylalanine ammonia-lyase (PAL) penetration into the monolayer of liver cells after its release from capsules was studied. The studies showed the absence of the effect of the capsule shell based on plant hydrocolloids on the absorption of l-phenylalanine ammonia-lyase in systems simulating the liver surface. After 120 min of incubation, in all variants of the experiment, from 87.

Li/Na substitution and Yb co-doping enabling tunable near-infrared emission in LiInSbO:Cr phosphors for light-emitting diodes.

Near-infrared (NIR) phosphor-converted light-emitting diode (pc-LED) has great potential in non-invasive detection, while the discovery of tunable broadband NIR phosphor still remains a challenge. Here, we report that Cr-activated LiInSbO exhibits a broad emission band ranging from 780 to 1400 nm with a full width at half maximum (FWHM) of 225 nm upon 492 nm excitation. The emission peaks are tuned from 970 to 1020 nm together with considerable broadening of FWHM (∼285 nm) via Li/Na substitution.