Robust intra-individual estimation of structural connectivity by Principal Component Analysis.

Fiber tractography based on diffusion-weighted MRI provides a non-invasive characterization of the structural connectivity of the human brain at the macroscopic level. Quantification of structural connectivity strength is challenging and mainly reduced to "streamline counting" methods. These are however highly dependent on the topology of the connectome and the particular specifications for seeding and filtering, which limits their intra-subject reproducibility across repeated measurements and, in consequence, also confines their validity.

Possible Mechanisms of Axonal Transport Disturbances in Mouse Spinal Motoneurons Induced by Hypogravity.

The data obtained by transcriptome analysis of lumbar spinal cord segments, sciatic nerve, and the respiratory diaphragm of the mice performed after a space flight on board Bion-M1 biosatellite were processed by bioinformatic methods aimed at elucidation of the regularities in hypogravity-induced transcriptome changes in various compartments of motor neurons. The study revealed abnormalities of axonal transport in spinal motor neurons provoked by weightlessness. These data agree with the results of electron microscopy examination of the spinal cord in experimental animals.

Epidural Stimulation Combined with Triple Gene Therapy for Spinal Cord Injury Treatment.

The translation of new therapies for spinal cord injury to clinical trials can be facilitated with large animal models close in morpho-physiological scale to humans. Here, we report functional restoration and morphological reorganization after spinal contusion in pigs, following a combined treatment of locomotor training facilitated with epidural electrical stimulation (EES) and cell-mediated triple gene therapy with umbilical cord blood mononuclear cells overexpressing recombinant vascular endothelial growth factor, glial-derived neurotrophic factor, and neural cell adhesion molecule. Preliminary results obtained on a small sample of pigs 2 months after spinal contusion revealed the difference in post-traumatic spinal cord outcomes in control and treated animals.

Identification of three new HLA-DQB1 alleles: HLA-DQB1*03:02:33, -DQB1*04:79 and -DQB1*05:249.

Three new DQB1*03:02:33, DQB1*04:79 and DQB1*05:249 alleles detected in Russian individuals by next-generation sequencing.

Characterization of three new HLA-A*11 alleles in Russian individuals by next-generation sequencing.

Three new HLA-A*11:12:02, A*11:01:104 and A*11:355:02 alleles detected in Russian individuals.

Two novel HLA-B variants identified in Russian individuals, HLA-B*56:74 and -B*58:124.

HLA-B*56:74 and -B*58:124, two novel alleles detected in Russian individuals by next-generation sequencing.

Characterization of the new HLA-B*35:482, detected in a potential hematopoietic stem cell donor.

One nucleotide substitution in codon 4 of HLA-B*35:01:01:01 results in a novel allele, HLA-B*35:482.

HLA-A*02:934, identified in National bone marrow donor registry named after Vasya Perevoshchikov, Russian Federation.

HLA-A*02:934 has one nucleotide change from HLA-A*02:01:01:01 at nucleotide 308.

Terbium(III)-thiacalix[4]arene nanosensor for highly sensitive intracellular monitoring of temperature changes within the 303-313 K range.

The work introduces hydrophilic PSS-[Tb(TCAn)] nanoparticles to be applied as highly sensitive intracellular temperature nanosensors. The nanoparticles are synthesized by solvent-induced nanoprecipitation of [Tb(TCAn)] complexes (TCAn - thiacalix[4]arenes bearing different upper-rim substituents: unsubstituted TCA1, tert-buthyl-substituted TCA2, di- and tetra-brominated TCA3 and TCA4) with the use of polystyrenesulfonate (PSS) as stabilizer. The temperature responsive luminescence behavior of PSS-[Tb(TCAn)] within 293-333 K range in water is modulated by reversible changes derived from the back energy transfer from metal to ligand (M* → T) correlating with the energy gap between the triplet levels of ligands and resonant D level of Tb ion.

Supraamphiphilic Systems Based on Metallosurfactant and Calix[4]resorcinol: Self-Assembly and Drug Delivery Potential.

Metallic amphiphiles are used as building blocks in the construction of nanoscale superstructures, where the hydrophobic effects induce the self-assembly of the nanoparticles of interest. However, the influence of synergizing multiple chemical interactions on an effective design of these structures mostly remains an open question. In this regard, supraamphiphilic systems based on flexible surfactant molecules and rigid macrocycles are being actively developed, but there are few works on the interaction between metallosurfactants and macrocycles.

The Effects of Repeated Administration of the Micellar Complex of Methylprednisolone on the Locomotor Activity of a Terrestrial Snails.

We studied the effects of repeated injections of methylprednisolone and its micellar complex with block-copolymer on locomotor activity of a terrestrial snail. It was shown that methylprednisolone solution injected into the hemolymph of the animal produced a direct effect on the muscle system of the animal as soon as 1 h after administration: it slowed down snail locomotion and reduced contractile activity of the foot muscles. The micellar complex of methylprednisolone with block-copolymer prevented this effect during the first 2 days of injection and negatively affected locomotion only in 2 days after injection, the decrease in locomotion in this case was not accompanied by a decrease in contractile activity of the foot muscle.

[Questions to experts when appointing a commission of forensic medical examinations based on adverse outcomes of medical care].

Presented the reviews of the recommendations to the forensic community on raising questions when prescribing forensic medical examinations in case of adverse outcomes of medical care is given. Were analyzed 50 decisions of investigators of the Investigative Committee of the Russian Federation on the conduct of forensic medical examinations in the regional bureaus of forensic medical examination, as well as non-state experts.

Supramolecular Organization As a Factor of Ribonuclease Cytotoxicity.

One of the approaches used to eliminate tumor cells is directed destruction/modification of their RNA molecules. In this regard, ribonucleases (RNases) possess a therapeutic potential that remains largely unexplored. It is believed that the biological effects of secreted RNases, namely their antitumor and antiviral properties, derive from their catalytic activity.

Effect of magnetite on anaerobic digestion of distillers grains and beet pulp: Operation of reactors and microbial community dynamics.

It has been previously shown that magnetite (FeO) nanoparticles stimulate the anaerobic digestion process in several anaerobic reactors. Here we evaluate the effect of magnetite nanoparticles on the efficiency of anaerobic digestion of distillers grains with solubles and sugar beet pulp in mesophilic batch experiments. The addition of magnetite nanopowder had a positive effect on the anaerobic digestion process.

Data on the genome and proteome profiles of ciprofloxacin-resistant strains selected under different conditions .

is widespread hypermutable bacteria (class Mollicutes) capable of infecting humans, animals, plants, which is the main contaminant of cell cultures and vaccine preparations. The mechanisms of the development of antimicrobial resistance of this bacterium are associated with the secretion of extracellular vesicles, which can mediate the lateral transfer of antibiotic resistance determinants. We compared the genome profiles of ciprofloxacin-resistant strains PG8r1 (MIC 10 µg/ml) and PG8r3 (MIC 10 µg/ml) selected under different conditions - when ciprofloxacin-sensitive (MIC 0.

Extracellular Matrix in Neural Plasticity and Regeneration.

The extracellular matrix (ECM) is a fundamental component of biological tissues. The ECM in the central nervous system (CNS) is unique in both composition and function. Functions such as learning, memory, synaptogenesis, and plasticity are regulated by numerous ECM molecules.

Detection of the HLA-B*51:322 allele in a Russian individual.

HLA-B*51:322 differs from B*51:01:01:05 by one nucleotide at position 1043C > T resulting in valine at codon 324.

Vasoactive Intestinal Peptide Changes the Frequency and Force of Myocardial Contraction in Rats.

We studied the effect of non-selective agonist of VIP receptors of vasoactive intestinal polypeptide in different concentrations on the frequency, force, and duration of isometric contraction of myocardial strips of the right atrium under conditions of spontaneous activity, as well as the force and duration of contractions of the right ventricle in rats. It was found that the agonist produced a positive inotropic and chronotropic effect that depended on its concentration. The maximum effect was observed at vasoactive intestinal peptide concentration of 10 M.

[Genetics of epilepsy: successes, problems and development prospects].

The authors present a detailed review of current advances in the field of genetics of epilepsy. Separately, new views on the etiology and pathogenesis of genetic epileptic encephalopathies, focal epilepsy and idiopathic generalized epilepsies are examined. The authors emphasize the importance of genetic discoveries for the clinical practice, including the prospects in the development of patients' personalized treatment.

Combined Supra- and Sub-Lesional Epidural Electrical Stimulation for Restoration of the Motor Functions after Spinal Cord Injury in Mini Pigs.

This study evaluates the effect of combined epidural electrical stimulation (EES) applied above (C5) and below (L2) the spinal cord injury (SCI) at T8-9 combined with motor training on the restoration of sensorimotor function in mini pigs. The motor evoked potentials (MEP) induced by EES applied at C5 and L2 levels were recorded in soleus muscles before and two weeks after SCI. EES treatment started two weeks after SCI and continued for 6 weeks led to improvement in multiple metrics, including behavioral, electrophysiological, and joint kinematics outcomes.

The novel HLA-A*02:941 allele was identified during high-resolution HLA typing.

HLA-A*02:941 has one synonymous nucleotide change from HLA-A*02:07:01 at nucleotide 292.

[Efficiency of RNA Hydrolysis by Binase from Bacillus pumilus: The Impact of Substrate Structure, Metal Ions, and Low Molecular Weight Nucleotide Compounds].

Binase is an extracellular guanyl-preferring ribonuclease from Bacillus pumilus. The main biological function of binase is RNA degradation with the formation of guanosine-2',3'-cyclic phosphate and its subsequent hydrolysis to 3'-phosphate. Extracellular RNases are believed to be key agents that affect the functional activity of the body, as they directly interact with epithelial and immune cells.

Draft Genome Sequence of the Plant Growth-Promoting Bacterium Bacillus subtilis Strain BZR 517, Isolated from Winter Wheat, Now Reclassified as Bacillus velezensis Strain BZR 517.

strain BZR 517 is a prospective plant growth-promoting rhizobacterium with known biocontrol properties, which may be used to improve soil quality. The genome sequencing was conducted as part of new biological agent development in order to determine the biocontrol potential of the strain, including the production of biologically active compounds.

The novel allele HLA-A*11:362 identified in two unrelated bone marrow donors from Russia.

HLA-A*11:362 has single nucleotide substitution at position 53G > C when compared to HLA-A*11:01:01:01.

Identification of the novel HLA-DQB1, allele DQB1*06:02:44 by next-generation sequencing.

Identification of the novel HLA-DQB1*06:02:44 allele that differs from HLA-DQB1*06:02:01 at one position in exon 2.