Newly Designed PCL-Wrapped Cryogel-Based Conduit Activated with IKVAV Peptide Derivative for Peripheral Nerve Repair.

The combination of macroporous cryogels with synthetic peptide factors represents a promising but poorly explored strategy for the development of extracellular matrix (ECM)-mimicking scaffolds for peripheral nerve (PN) repair. In this study, IKVAV peptide was functionalized with terminal lysine residues to allow its in situ cross-linking with gelatin macromer, resulting in the formation of IKVAV-containing proteinaceous cryogels. The controllable inclusion and distribution of the peptide molecules within the scaffold was verified using a fluorescently labelled peptide counterpart.

Influence of Urinary Urea Concentration on Hemolytic Activity and Cytotoxicity of Uropathogenic Morganella morganii Strain.

We studied the effect of urinary urea concentration on the hemolysin production and cytotoxicity of the uropathogenic Morganella morganii strain MM 190. The highest hemolytic activity of M. morganii cultivated in urine with low urea concentration (23 and 82 mmol/liter) was observed between 3rd and 4th hours of post-inoculation, while in urine with standard urea level (117 mmol/liter), the activity was observed at 5th hour of post-inoculation.

Changes in the Levels of Short-Chain Acids and Tryptophan Metabolites in Mouse Intestine during Experimental Colitis.

The optimal balance of the intestinal microbiota is considered to be an essential part of the human body that affects many metabolic processes. However, the exact role of the gut microbiota in metabolism is still not fully understood. To investigate the metabolic role of gut microbiota, the content of short-chain fatty acids and tryptophan metabolites was studied in mice with sodium dextran sulfate-induced colitis.

[Activation of endogenous mechanisms of sanogenesis in cognitive impairment in cerebral small vessel disease].

Cerebrovascular diseases themselves are the second most common cause of cognitive impairment (dementia). In addition, cerebral small vessel disease (CSVD) makes a significant contribution to the clinical picture of neurodegenerative diseases. Since there are currently no pharmacological treatments for CSVD, a promising method is the activation of the endogenous mechanisms of sanogenesis.

[Metabolic Profile of Gut Microbiota and Levels of Trefoil Factors in Adults with Different Metabolic Phenotypes of Obesity].

Obesity is associated with changes in the gut microbiota, as well as with increased permeability of the intestinal wall. In 130 non-obese volunteers, 57 patients with metabolically healthy obesity (MHO), and 76 patients with metabolically unhealthy obesity (MUHO), bacterial DNA was isolated from stool samples, and the 16S rRNA gene was sequenced. The metabolic profile of the microbiota predicted by PICRUSt2 (https://huttenhower.

Identification of a Novel FLT4 c.3028A>C Variant Associated With Milroy Disease.

VEGFR3 (FLT4) is crucial for embryonic lymphangiogenesis, and defects in this receptor can lead to congenital lymphedema type 1A (Milroy disease). This study analyses FLT4 gene sequence in 24 primary lymphedema patients, identifying genetic variants in five patients resembling typical Milroy disease. A novel likely pathogenic variant (c.

Guest inclusion by native cyclodextrins in solid state and solutions: A review.

In many industrial applications, preparation of cyclodextrin (CD) inclusion complexes with drugs, food additives, dyes and components of essence oils is performed in solid mixtures, slurries or paste-like systems having lack of water to dissolve cyclodextrin and guest completely. Such systems need a different description than supplied by classical analysis of CD complexation in aqueous solutions. The main feature of solid-state guest inclusion is the phase transition from solid CD to solid inclusion compound.

Iminoglutamic succinic acid: assessment of the degree of biodegradation and toxicity of half-decomposition products.

Chelating agents used in agriculture and land reclamation practices must exhibit the capacity to firmly bind metal ions while also rapidly decomposing into non-toxic compounds due to their introduction into the environment in substantial quantities. It is therefore crucial to identify an alternative to EDTA, a prevalent chelating agent known for its low biodegradability and relatively high toxicity. This study focuses on assessing the degree of biodegradation of iminoglutamic succinic acid (IGSA) and the toxicity of its half-decomposition products.

Characterisation of Four Novel HLA Alleles: -A*02:01:222, -B*07:02:104, -C*07:02:156 and -DQB1*06:03:51.

Four novel HLA alleles HLA-A*02:01:222, -B*07:02:104, -C*07:02:156 and -DQB1*06:03:51 alleles detected during routine next generation sequencing.

Two Novel HLA-С Alleles, HLA-C*07:1153 and -C*07:1154, Detected During Routine Next Generation Sequencing.

Two novel HLA-С alleles HLA-C*07:1153 and -C*07:1154 alleles detected during routine next generation sequencing.

Cryostructuring of Polymeric Systems: 68. Evaluation of Poly(vinyl alcohol) Composite Cryogels Filled with Poly(3-hydroxybutyric acid)-Based Microspheres of Different Porous Morphology as Potential Delivery Systems for Drugs of Various Water-Solubility.

Poly(3-hydroxybutyric acid)-based microspheres of two types, with and without macropores, were prepared; their morphology and particle size were evaluated. These microspheres were entrapped as disperse fillers into the bulk of macroporous cryogels based on poly(vinyl alcohol) (PVA). It was found that the rigidity of the resultant composite cryogels increased markedly as compared to that of unfilled cryogels of the same PVA concentration.

Preparation of amorphous Nd/Dy-based metal organic framework@MXene for solar driven selective photocatalytic and serving as sensor for fluorescence quenching detection, and biological activity.

The development of a new Neodymium/Dysprosium metal-organic framework, referred to as Nd/Dy-BTC MOF, based on benzene-1,2,4-tricarboxylic acid, has been achieved through an in situ growth process on 2D transition metal carbides (MXene) surfaces. This synthesis was conducted via a solvothermal method utilizing a solvent mixture of water, ethanol, and dimethylformamide. The primary objective of this research is to investigate the framework's efficacy as a photocatalyst for the degradation of anionic dye, as well as its potential for sensing certain explosives.

Identification of Giant Isoforms of Obscurin in Rat Striated Muscles Using Polyclonal Antibodies.

Using produced polyclonal antibodies specific to the N-terminal sequence (residues 61-298) of rat obscurin, we investigated the isoform composition of this protein in 4 striated muscles: myocardium of the left ventricle, diaphragm, skeletal m. gastrocnemius (containing mainly fast fibers), and m. soleus (containing mainly slow fibers).

Cyclization of alanyl-valine dipeptides in the solid state. The effects of molecular radiator and heat capacity.

Heating of linear dipeptides above a critical temperature initiates their cyclization even in the solid state. This method of obtaining cyclic dipeptides meets the requirements of "green chemistry", provides a high yield of the main product and releases only water as a by-product of the reaction, and does not require solvents. However, to date, the cyclization of only a small number of dipeptides in the solid state has been studied, and some correlations of the process were discovered.

Differential expression of quick-to-court gene isoforms in Drosophila male and female.

The quick-to-court (qtc) gene is expressed in both males and females but affects only the mating behavior of males, probably due to the different composition of isoforms between the sexes. We tested this hypothesis and examined the sex-specific expression of qtc transcripts in the tissues of male and female Oregon-R flies. It was found that some qtc transcripts, such as qtc-RM and qtc-RN, are testis-specific, while others like qtc-RH are found in ovaries but absent in testes.

[Prevalence of gastroesophageal reflux disease in Russia: a meta-analysis of population-based studies].

Aim: To systematize data on the prevalence of gastroesophageal reflux disease (GERD) in the adult population of Russia.

Materials And Methods: The search for studies was conducted in the electronic databases MEDLINE/PubMed, EMBASE, and RSCI (Russian Science Citation Index) from January 2000 to December 2022. The review included relevant publications in peer-reviewed periodicals in English or Russian, publications with data from cross-sectional epidemiological studies assessing the prevalence of GERD in the Russian population, studies on adult patients with GERD, and publications with detailed descriptive statistics that allow using the data in the meta-analysis.

[The prevalence of mutations underlying development of Helicobacter pylori resistance to antibiotics in Kazan].

Background: One of the reasons for the decrease of eradication effectiveness is its resistance to antibiotics.

Aim: To examine the prevalence of point mutations responsible for clarithromycin and levofloxacin resistance among the patients with upper gastrointestinal (GI) tract disorders in Kazan.

Materials And Methods: The study included 203 patients with symptoms of dyspepsia who underwent upper GI endoscopy at the University Hospital of Kazan Federal University (Kazan, Russia) in 2019-2021.

Influence of flaxseed mucilage on the formation, composition, and properties of exopolysaccharides produced by different strains of lactic acid bacteria.

Microorganisms produce a wide variety of polysaccharides. Due to biosafety considerations, lactic acid bacteria (LAB) are popular producers of exopolysaccharides (EPS) for various applications. In this study, we analyzed the composition and properties of EPS produced by L.

Functional and Structural Changes in Diaphragm Neuromuscular Junctions in Early Aging.

Age-related impairment of the diaphragm causes respiratory complications. Neuromuscular junction (NMJ) dysfunction can be one of the triggering events in diaphragm weaknesses in old age. Prominent structural and functional alterations in diaphragm NMJs were described in elderly rodents, but NMJ changes in middle age remain unclear.

The Combined Delivery of the , , and Genes Stimulates Angiogenesis and Improves Post-Ischemic Innervation and Regeneration in Skeletal Muscle.

In this study, the effects of different combinations of the genes , , and injected both using direct virus-mediated injection (adenovirus, Ad5) and umbilical cord blood mononuclear cells (UCBCs) on the processes of stimulation of post-ischemic innervation, angiogenesis, and regeneration in skeletal muscle were investigated in a rat hindlimb chronic ischemia model. It was shown that more pronounced stimulation of angiogenesis and restoration of post-ischemic innervation were achieved both in the early (28 days post-ischemia, dpi) and late (42 dpi) terms of the experiment in the calf muscle when UCBCs delivered the combination of Ad5- and Ad5- compared to the direct injection of the same vector combination into the area of ischemia. At the same time, the inclusion of Ad5- in the combination of Ad5- and Ad5- directly injected or administered by UCBCs provided a significant increase in the number of centronuclear muscle fibers, indicating stimulation of post-ischemic reparative myogenesis.