Three theses on thrombosis of the veins.

Thrombosis of veins may be 1. the consequence of damaged endothelial cells, 2. the result of a synergism between local stasis and hypercoagulable blood and 3.

Diagnosis and differentiation of von Willebrand's disease type II.

Out of 113 patients with vWD 15 were of type II. The basic test programme included F VIII:C, vWF-Ag, RCF and BT (Ivy). All type II patients had relatively high vWF-Ag and low RCF values.

Anti-dextran antibodies of carp detected by the enzyme-linked immunosorbent assay (ELISA).

Carp produce anti-alpha (1-6) dextran antibodies after immunization with a vaccine of Leuconostoc mesenteroides B512. These antibodies can be determined by passive haemagglutination, quantitative precipitation or by ELISA. In our hands the ELISA has proved to be 100 times more sensitive than the passive haemagglutination test.

Analysis of human exocrine pancreatic proteins by means of pore gradient polyacrylamide gel electrophoresis.

Pancreatic juice collected by endoscopic retrograde cannulation of the pancreatic duct after administration of secretin and pancreozymin was analyzed by pore gradient polyacrylamide gel electrophoresis. Two electrophoretic systems, an alkaline (pH 8.9) and an acidic (pH 5.

Immune complex induced hematogenic cell infiltration of the aortic luminal border layer in rabbits. Light microscopic studies of surface preparations.

Interendothelial and subendothelial monocytes/macrophages are extremely rare in normal rabbit aortas compared with humans and swine, as has been shown by the present study and previous investigations of surface preparations. Light microscopy was used to study the development of immune complex-induced hematogenic macrophage infiltrations in 26 rabbits from the 4th to the 28th day after the last antigen administration. Of particular interest was the intensity, localization and duration of the hematogenic cell infiltrates with a view to establishing the role of immunological stimuli for subendothelial monocyte and lymphocyte accumulation.

Influence of beta-carotene supplementation on carotene content of ovaries of heifers.

Carotene is stored as a tissue reserve in the corpus luteum. With different carotene supplementations in heifers (0, 100, 200 and 300 mg/animal and day) it could be clearly seen that the carotene concentration in the corpus luteum (2.3, 27, 50 and 81 micrograms/g) was directly dependent on it.

Fructose-2,6-bisphosphatase and 6-phosphofructo-2-kinase are separable in yeast.

Fructose-2,6-bisphosphatase was purified from yeast and separated from 6-phosphofructo-2-kinase and alkaline phosphatase. The enzyme released Pi from the 2-position of fructose 2,6-bisphosphate and formed fructose 6-phosphate in stoichiometric amounts. The enzyme displays hyperbolic kinetics towards fructose 2,6-bisphosphate, with a Km value of 0.

Cholestasis in late metabolic acidosis of prematurely born infants.

Serum concentrations of bile acids and tyrosine were determined in 14 premature infants with late metabolic acidosis and in 13 comparable controls without acidosis (protein intake 2 g/kg X d). At the same time the bile acids and the catalytic activity concentrations of lipase and trypsin were estimated in the duodenal juice. The daily faecal excretion and the percentage of fat eliminated were measured.

The higher myosin ATPase activity in the right heart ventricle of the rat, proved by histophotometry.

Comparing the myosin-ATPase activity in the left and right ventricular wall of the hearts from 6 rats by scanning histophotometry, a higher enzyme activity in the right ventricle was found. These findings are in a good agreement with the higher numbers of myofilaments per myofibril in the right ventricle, reported in literature.

Histophotometrical measurements concerning the distribution of myofibrillar ATPase activity within the tissue block after aldehyde fixation.

The inhibition of myofibrillar ATPase activity by aldehyde fixation in tissue pieces of myocardium of the rat was measured by scanning histophotometry. The relative amount of the final reaction product of the histochemical method for ATPase (Padykula and Herman 1955) was taken as a measure for the enzyme activity. Along scanning lines in slides from the surface to the centre of tissue block, a higher activity was found in the centre in contrast to the marginal zone.

Sepharose gels as antigenic matrices for specific binding of anti-galactosyl antibodies.

From 100 ml of pooled normal serum taken from different species (man, cow, rabbit, chicken, duck, carp) 0.5 to 4.0 mg of high and low molecular weight anti-galactosyl antibodies could be isolated by affinity-chromatography on non-ligand-attached Sepharose 4B.

Molecular absorption spectrometry (MAS) by electrothermal evaporation in a graphite furnace-XIII Determination of traces of fluoride by mas of AlF after liquid-liquid extraction of fluoride with triphenylantimony(V) dihydroxide.

The determination of traces of fluoride by means of the molecular absorption of AlF volatilized in graphite cuvettes is described. An extraction method for separation and preconcentration of the fluoride has been developed, to avoid matrix effects. The fluoride is extracted with 10(-3)M triphenylantimony(V) dihydroxide in M1BK, and stripped with 0.

Investigations of trace analysis of A(III)B(V) semiconductor microsamples by atomic spectroscopy-VII Investigation of trace and thin-layer analysis of doping elements (Ag, Au, Bi, Cd, Sn, Tl) in InAs by atomic-absorption with electrothermal evaporation.

Etching procedures for separation of thin layers of InAs (up to 1.4 mum thick) have been developed and optimized. A solid-state microtome has been used for cutting layers thicker than 1mum.

Molecular absorption spectrometry (MAS) by electrothermal evaporation in a graphite furnace-IX Determination of traces of bromide by mas of AlBr after liquid-liquid extraction of bromide with triphenyltin hydroxide.

The determination of traces of bromide by molecular absorption spectrometry (MAS) of AlBr (with electrothermal volatilization) is described. It is possible to determine 25 ng of bromide. Many problems are caused by various matrices, so an extraction method for separation (and also preconcentration) was developed.

Complexometric titrations controlled by anodic-stripping methods-I Voltammetric stripping.

Anodic-stripping voltammetry is used to perform automatic complexometric titrations of metal ions, with high precision. The stripping peaks are converted into corresponding titrant volume increments which are added consecutively during stripping. In analysis of samples containing about 1 mmole, each of lead, indium and gallium, relative standard deviations of 0.

The tiron radical as indicator substance in catalytic determination of trace metals.

The tiron-hydrogen peroxide reaction used for catalytic determination of trace metals is studied with respect to the indicator substance-the oxidation product of tiron absorbing at 44Onm. Contrary to assumptions in the literature, it is shown by electrochemical techniqus EPR spectroscopy and visible spectrophotometry that the indicator substance is not the o-quinone derivative of tiron (lambda(max) = 372 nm) but the tiron semiquinone radical with a g-value of g(o) = 2.0049 and molar absorptivity = (3.