First results on the incorporation and excretion of 15N from orally administered urea in lactating pony mares.

Two lactating pony mares were given oral offers of 20 g 15N urea [95 atom-% 15N-excess (15N')] on 6 subsequent days. About 80% of the consumed 15N' were excreted via urine and faeces, but only about 2% via milk. The 15N' secreted via milk-lysine only amounted to 0.

Biochemical and morphological studies on perivenous and periportal liver parenchymal cells from female rats isolated by digitonin-collagenase method.

Periportal (pp) or perivenous (pv) liver parenchymal cells from female adult Uje: WIST rats were isolated after retro- or antegrade digitonin infusion followed by collagenase perfusion in the opposite direction. The morphological results revealed a distinct acinar-related destruction of the pv- or pp-zone by digitonin. The remaining cells of the respective other zone showed a good structural maintenance.

Cholinergic neurons of the central nervous system: morphofunctional aspects.

Impairment of central cholinergic function is an early and constant finding in a number of mental disorders associated with amnesia or dementia. Although knowledge of the detailed functional implications of cholinergic mechanisms in cognition is still very incomplete, some recent results and concepts about the morphofunctional organization of the cholinergic basalo-cortical projection system are reviewed. This cholinergic system is quite different from other so-called unspecific subcortical projection systems and might have some bearing on understanding the role of cholinergic mechanisms in cognitive function and its disorders.

Effect of varying glucosinolate and iodine intake via rapeseed meal diets on serum thyroid hormone level and total iodine in the thyroid in growing pigs.

In a trial with 50 fattening pigs (20 kg initial body weight), the effect of untreated rapeseed meal (RSM) (148 mmol glucosinolates and aglucones per kg dry matter) on the thyroid was compared with RSM treated with Cu2+ (9.5 mmol glucosinolates and aglucones per kg dry matter) and soybean meal (SBM). The diets containing 8% RSM were supplemented with 0.

Description of a simple method to study some aspects of the pathogenesis of arteriosclerosis by perfusion of arterial segments.

A perfusion apparatus is described which permits the perfusion of arterial segments of about 5 cm in length. It works under physiological conditions. During the perfusion, temperature and pressure constancy or pressure variation, respectively, pulsation, sufficient oxygen supply, and high sterility are guaranteed.

Influence of ethanol pretreatment of differing duration on toxic effects of carbon tetrachloride in rats.

Serum activities of alanine-aminotransferase (ALAT, EC 2.6.1.

Interleukin-2 receptor expression and interleukin-2 production in bullous pemphigoid.

Expression of interleukin-2 (IL2) receptors was studied on peripheral blood lymphocytes (PBLs) in 25 patients with bullous pemphigoid. Analysis was carried out by flow cytometry. Without immunosuppressive therapy expression of IL2 receptors only on T cells (CD5) was significantly increased as shown by double staining (11.

On the action of hypochlorite on human serum albumin.

The luminol-enhanced chemiluminescence of zymosan-stimulated polymorphonuclear leukocytes is continuously diminished in the presence of increasing amounts of human serum albumin (from 1 to 30 mg/ml). HSA competes with luminol for hypochlorite as shown by adding sodium hypochlorite to luminol solutions containing HSA. Titration of HSA with NaOCl affects firstly the sulfhydryl and probably the thioether groups and then the amino groups.

Dose-reduced induction therapy for acute leukaemias decreases both the complete remission (CR) rate and the probability of leukaemia-free survival (LFS).

Especially in AML but also in ALL a dose reduction during the induction therapy effected distinctly both a diminution of the CR rate and a shortening of the LFS. For these reason reduced treated patients are to exclude from final analysis of study in order to obtain a objective comparison of the four postremission treatment modalities. There was no difference concerning treatment related mortality between "correct" and "reduced" induction therapy.

Affinity partitioning and extraction of proteins.

Affinity partitioning of enzymes and plasma proteins in aqueous two-phase systems has been reviewed. Besides basic theoretical considerations of the principle of affinity partitioning the chemistry of coupling ligands to the polymers, the nature and properties of selected biomimetic ligands like dye-ligands, immunoligands, metal chelate ligands and hydrophobic ligands are reported. The usefulness of affinity partitioning for studying the affinity of ligands and their specificity to proteins has been demonstrated by selected examples.

An improved purification procedure of alkaline phosphatase from calf intestine by applying partition in aqueous two-phase systems and dye-ligand chromatography.

Aqueous two-phase partitioning has been elaborated in order to improve the purification of alkaline phosphatase from calf intestine in larger scale. The laborious precipitation and centrifugation steps for the removal of the enzyme from the cell debris and from the bulk protein were replaced by this technique yielding a high recovery (88%) and a significant lower time requirement. For the preparation of 100.

Interaction of lactate dehydrogenase with structurally related triazine dyes using affinity partitioning and affinity chromatography.

Affinity partitioning in aqueous two-phase systems consisting of dextran and dye-liganded polyethylene glycol was employed to study the interaction of lactate dehydrogenase (LDH) from rabbit muscle (E.C. 1.

Ducto-insular proliferation of beta-cells after syngeneic islet transplantation into the spleen of streptozotocin-diabetic Lewis rats.

Syngeneic transplantation of cultured and functionally characterized neonatal islets into the spleen of streptozotocin diabetic Lewis rats resulted in long time survival up to 200 d and in plasma glucose levels lower than 9 mmol/L. The daily plasma glucose profile of transplanted rats had shown significantly above that of nondiabetic control rats. Two-hundred d after transplantation, morphologically intact, insulin containing beta-cells were demonstrable in the spleen, thus demonstrating the long-term survival of functioning islet cells.

Purification and properties of carnitine dehydratase from Escherichia coli--a new enzyme of carnitine metabolization.

Carnitine dehydratase from Escherichia coli 044 K74 is an inducible enzyme detectable in cells grown anaerobically in the presence of L(-)-carnitine or crotonobetaine. It has been purified 500-fold to electrophoretic homogeneity by chromatography on phenyl-Sepharose, hydroxyapatite, DEAE-Sepharose, second phenyl-Sepharose and finally gel filtration on a Sephadex G-100 column. During the purification procedure a low-molecular-weight effector essential for enzyme activity was separated from the enzyme.

beta-Casomorphins alter the intestinal accumulation of L-leucine.

Everted sacs of the rat jejunum change the accumulation of [3H]leucine when beta-casomorphins (BCMs) or synthetic analogs, in a concentration range of 10(-8) mol/l, are coincubated with the amino acid. BCM5 (BCM fragment 1-5, Tyr-Pro-Phe-Pro-Gly) and [D-Ala2]-BCM5-NH2 (Tyr-D-Ala-Phe-Pro-Gly) increase, whereas [D-Pro4]-BCM5 (Tyr-Pro-Phe-D-Pro-Gly) decreases the leucine accumulation and [Arg8]-vasopressin has no effect. No effect of BCM5 could be observed on the accumulation of the space marker [14C]inulin.

The ontogeny of benzodiazepine receptors in selected regions of the rat brain: effect of perinatal exposure to diazepam.

The postnatal development of the binding of [3H]flunitrazepam to benzodiazepine receptors has been studied in the frontal cortex, cerebellum, striatum, hypothalamus and hippocampus of the rat after prenatal, perinatal and postnatal exposure to diazepam. The dams were injected subcutaneously with single daily doses of 1 mg/kg of diazepam from day 7-20 of gestation or from day 15 of gestation day 6 after birth. Offspring of untreated dams were injected in the same way from postnatal day 7-20.

Affinity chromatography of bovine heart lactate dehydrogenase using dye ligands linked directly or spacer-mediated to bead cellulose.

A number of reactive dyes coupled to bead cellulose directly or spacer-mediated has been investigated in respect of their interaction with lactate dehydrogenase (LDH; E.C. 1.

Field studies towards intestinal helminthoses from north Ethiopia (1982) by means of microscopic and seroimmunologic methods.

In two villages of north Ethiopia (Gorgora and Zerema) helminthologic stool analyses were performed and compared with seroimmunologic examinations. Regional difference could be obtained. The infection rate of males was slightly higher than that of females (except Schistosomiasis in Gorgora).

The course of axons within the postnatal rabbit retina.

The course of optic axons within the postnatal rabbit retina was studied by scanning electron microscopy (SEM) of the nerve fibre layer exposed by HCl and collagenase treatment, and by freeze-fracture electron microscopy. In the first days after birth, axon bundles were found to run along a rather tortuous way in between the developing Müller cell endfeet. Later on and in adults, the axon bundles show a more straight course.

Temperature effects on isometric contractions of slow and fast twitch muscles of various rodents--dependence on fibre type composition: a comparative study.

The influence of temperature (range 15-37 degrees C) on the isometric contractions of the slow twitch soleus (SOL) and the fast twitch extensor digitorum longus (EDL) muscles of mice, rats and guinea pigs were investigated in vitro. Cooling of the bathing solution prolonged the time parameters of single twitches and tetanic contractions in a non-linear manner in both muscle types of all animals. In muscles containing predominately fast twitch fibres like the EDL of all animals cooling was followed by an increase of the single twitch tension (cold potentiation) with a maximum of 160-180% at about 20 degrees C.

Coordination processes in a human motoneuronal pool.

Using two leads of a multielectrode the EMG from biceps brachii muscle in man was recorded. The test-persons were ordered to produce a sustained isometric flexion force in the elbow joint which they could control optically. In the range of about 3-5% of maximum force it was possible to identify 2-5 active motor units under this condition.

The influence of suspension hypokinesia on contractile properties of slow and fast twitch muscles of young growing and adult rats.

The influence of the lack of the gravity force on contractile properties of the slow-twitch soleus (SOL) and the fast-twitch extensor digitorum longus (EDL) muscles was investigated in 3 week or 6-month-old rats suspended by their tail. In both groups suspension hypokinesia (SH) lasting 3 weeks or longer leads to a marked decrease of the mass and of the mean cross-sectional area of the SOL. The SOL of suspended animals shows decreased contraction and half-relaxation times and a lower tension of the single twitch, a decreased maximum tetanic force output, an increased fusion frequency and a decreased tetanus-half-rise time.

The influence of sulphur, molybdenum and cadmium exposure on the growth of goat, cattle and pig.

In growing billy goats, bulls and heifers, the supplementation of 10 g elementary S/kg ration dry matter reduced the daily weight gain by about 15%. The offer of 10 g S and 10 mg Mo/kg ration did not intensify growth depression. It occurred, however, when 10 g S were applied together with 3 mg Cd.

Shape and size of synaptic vesicles in different types of presynaptic profiles in the medial septal nucleus of the rat.

Long and short axis diameters of synaptic vesicles were obtained from 6 classes of presynaptic boutons found in the medial septal nucleus (MS) of the rat. The variability of vesicle size and shape was documented in diagrams and discussed with regard to their suitability as criteria of classification and their functional significance. The variability in shape, but not in size, of vesicles in synapses of Gray's type I is lower than of those in Gray's type II synapses.

A Golgi deimpregnation study of neurons in the rhesus monkey visual cortex (areas 17 and 18).

The morphological features of 298 neurons impregnated according to Golgi-Kopsch in areas 17 and 18 of Macaca mulatta were analyzed, and the same neurons were deimpregnated to visualize structural details of the somata in different types of neurons. The following cell types were investigated: Pyramidal and pyramid-like cells, spiny stellate cells, double bouquet cells, bipolar cells, chandelier cells, neurogliaform cells, basket and related cells. This procedure allows the evaluation of the nuclear-cytoplasmic proportion and the position of the nucleus besides shape and size of the cell body.