Screening of ocular enterobacteriaceae isolates for presence of chromosomal blaNDM-1 and ESBL genes: a 2-year study at a tertiary eye care center.

Purpose: Since, to our knowledge, there are no reports on the prevalence of the blaNDM-1 gene among ocular isolates of Enterobacteriaceae, and only limited information on the prevalence of extended spectrum beta-lactamases (ESBLs) among ocular bacterial isolates are available, our study was undertaken.

Methods: A prospective study was done on 74 Enterobacteriaceae isolates from patients presenting with clinical suspicion of bacterial ocular infections during a period from January 2010-December 2011. All isolates were subjected to detection of ESBLs by double disc synergy and screened for the presence of CTX-M -I, II, III, and IV groups, and OXA, TEM, SHV, blaNDM-1 genes by PCR.