5 results match your criteria: "KANKA Pediatric Hematology & Oncolgy Hospital[Affiliation]"
Outcomes of HLA-mismatched HSCT with TCRαβ/CD19 depletion or post-HSCT cyclophosphamide for inborn errors of immunity.
Blood
August 2024
Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne, United Kingdom.
HLA-mismatched transplants with either in vitro depletion of CD3+ T-cell receptor (TCR)αβ/CD19 (TCRαβ) cells or in vivo T-cell depletion using posttransplant cyclophosphamide (PTCY) have been increasingly used for patients with inborn errors of immunity (IEIs). We performed a retrospective multicenter study via the EBMT registry on 306 children with IEIs undergoing their first transplant between 2010 and 2019 from an HLA-mismatched donor using TCRαβ (n = 167) or PTCY (n = 139). The median age for hematopoietic stem cell transplantation (HSCT) was 1.
View Article and Find Full Text PDFProtocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells.
STAR Protoc
December 2022
Faculty of Science, Department of Biology, Erciyes University, Kayseri 38039, Türkiye; Genome and Stem Cell Center (GENKÖK), Erciyes University, Kayseri 38039, Türkiye. Electronic address:
Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells (PBMCs). We describe the steps for isolating target cells and their in-column surface biotinylation, followed by isolation and mass spectrometry analysis of biotinylated proteins.
View Article and Find Full Text PDFAssessment of extracorporeal photopheresis related cell damage.
Transfus Apher Sci
December 2022
Department of Pediatrics, Division of Pediatric Hematology Oncology, Bone Marrow Transplantation Center, Erciyes University Faculty of Medicine, KANKA Pediatric Hematology & Oncolgy Hospital, Kayseri, Turkey. Electronic address:
Aim: Extracorporeal photochemotherapy (ECP) is emphasized chiefly as it has a high safety profile. However, the genotoxic effects of ECP are not known. This experimental study aimed to assess the potential genotoxic impact of ECP treatment by the AKLIDES system, a new generation standardized and automated evaluation method.
View Article and Find Full Text PDFThalassemia-free and graft-versus-host-free survival: outcomes of hematopoietic stem cell transplantation for thalassemia major, Turkish experience.
Bone Marrow Transplant
May 2022
Acıbadem Adana Hospital, Pediatric Hematology and Stem Cell Transplantation Unit, Adana, Turkey.
We report the national data on the outcomes of hematopoietic stem cell transplantation (HSCT) for thalassemia major (TM) patients in Turkey on behalf of the Turkish Pediatric Stem Cell Transplantation Group. We retrospectively enrolled 1469 patients with TM who underwent their first HSCT between 1988 and 2020 in 25 pediatric centers in Turkey. The median follow-up duration and transplant ages were 62 months and 7 years, respectively; 113 patients had chronic graft versus host disease (cGVHD) and the cGVHD rate was 8.
View Article and Find Full Text PDFA teenager boy with a novel variant of Sitosterolemia presented with pancytopenia.
Clin Chim Acta
April 2022
Division of Pediatric Hematology and Oncology, Department of Pediatrics, KANKA Pediatric Hematology Oncology & HSCT Hospital, Erciyes University, Faculty of Medicine, Kayseri, Turkey; Department of Molecular Biology and Genetics, Gevher Nesibe Genom and Stem Cell Institution, Genome and Stem Cell Center (GENKOK), Erciyes University, Kayseri, Turkey; Department of Blood Banking and Transfusion Medicine, Health Science Institution, Erciyes University, Kayseri, Turkey. Electronic address:
Article Synopsis
- Sitosterolemia is a condition characterized by high levels of plant sterols due to increased absorption and reduced excretion, causing symptoms like xanthomas, anemia, and thrombocytopenia.
- A 10-year-old boy with pancytopenia and macrothrombocytopenia was studied, revealing a new genetic variant related to the ABCG5 gene, which is linked to Sitosterolemia.
- Treatment with a restricted diet and ezetimibe significantly lowered the patient's plant sterol levels, highlighting the importance of considering Sitosterolemia in children with subtle hematological signs.