Co-immobilization of laccase and zinc oxide nanoparticles onto bacterial cellulose to achieve synergistic effect of photo and enzymatic catalysis for biodegradation of favipiravir.

The environmental persistence of pharmaceuticals represents a significant threat to aquatic ecosystems and human health, while limitations in conventional wastewater treatment methods underscore the urgent need for innovative and eco-friendly degradation strategies. Photobiocatalytic approaches provide a promising solution for the effective degradation of pharmaceutical contaminants by harnessing the synergistic effects of both photocatalysts and biocatalysts. In this study, we developed a photobiocatalytic composite by co-immobilizing laccase enzyme and zinc oxide nanoparticles on bacterial cellulose synthesized from orange peel waste.

Giant Baker Cyst Extending Up to the Gastrocnemius: A Case Report.

Baker cyst is an abnormal enlargement of the gastrocnemius-semimembranous bursa behind the knee joint due to an exit of joint fluid. We herein report a rare case of giant Baker cyst in a rheumatic arthritic female patient. An MRI scan showed a complex, multiloculated cyst measuring 11.

Cloning, purification and characterization of a novel thermostable recombinant tannase from Galactobacillus timonensis.

The exorbitant production costs associated with natural tannases pose a significant challenge to their widespread industrial utilization. Microbial expression systems provide a cost-effective method for enzyme production. In this study, a putative gene encoding the subtype B tannase (Gt-Tan) was cloned from Galactobacillus timonensis and expressed heterologously in Escherichia coli BL21 (DE3) cells.

Comparison of clinical characteristics and outcomes in candidaemia patients with and without COVID-19: a multicentre retrospective study.

Background: Invasive fungal infections have been reported as complications with significant mortality and morbidity in patients hospitalized with COVID-19. This study aimed to evaluate the clinical characteristics and outcomes of candidaemia patients with COVID-19 and to investigate the association between COVID-19 and mortality in candidaemia patients.

Methods: This retrospective study included candidaemia patients aged 18 years or older admitted to four university-affiliated tertiary hospitals in South Korea between January 1, 2020, and December 31, 2022.

Computational and biochemical characterization of the immobilized esterase of Salinicoccus roseus for pesticide degradation.

The continuous exposure of chemical pesticides in agriculture, their contamination in soil and water pose serious threat to the environment. Current study used an approach to evaluate various pesticides like Hexaconazole, Mancozeb, Pretilachlor, Organophosphate and λ-cyhalothrin degradation capability of esterase. The enzyme was isolated from Salinicoccus roseus.

The discovery of a random endo-acting ι-carrageenase with bifunctional activities against ι-carrageenan and κ-carrageenan.

Carrageenans have attracted increasing research interests in recent decades for their various physicochemical and physiological properties. Random endo-acting carrageenases are promising tools for tailoring the molecular weight of carrageenan and preparing a series of carrageenan oligosaccharides. Although the processive ι-carrageenases in the GH82 family have been widely investigated, the random ι-carrageenase has not been reported.

Enhancing Stability and Catalytic Activity of d-Allulose 3-Epimerase through Multistrategy Computational Design and Cross-Regional Advantageous Mutations.

d-Allulose 3-epimerase (DAEase) derived from has excellent properties in the catalytic production of d-allulose, a rare sugar with unique biological functions. However, the industrial application of DAEase (Cb-DAEase) for d-allulose production is hindered by its low enzyme activity, poor long-term thermostability, and pH tolerance. In this study, we identified potential noncatalytic residues in Cb-DAEase using methods such as proline substitution, surface charge engineering, and surface residue prediction.

Muscle Metabolism During Multiple Muscle Stimulation Using an Affordable Equipment.

: Previous studies have shown that neuromuscular electrical stimulation (NMES), while expensive, can provide some of the health benefits of exercise to people who cannot exercise their legs normally. The aim of this study was to quantify the increases in muscle metabolism in four muscles of the legs of able-bodied individuals with NMES. : Healthy college-aged students were tested.

Bioinformatics Analysis of Autophagy-Related Genes in Kidney Transplantation.

Introduction: Autophagy related genes (ARGs) may play important roles in various biological processes involving kidney transplantation (KT); however, their expression characteristics are rarely used to study the relationship between autophagy and prognosis in KT patients. This study aims to construct a new autophagy related gene feature based on high-throughput sequencing datasets.

Methods: Differentially expressed ARGs (DEARGs) were identified in KT patients based on the Gene Expression Omnibus (GEO) database.

Identification and characterization of a novel chromosome-encoded aminoglycoside -nucleotidyltransferase gene, in sp. TYF-12 isolated from the marine fish intestine.

Background: The mechanisms underlying the resistance of the genus to aminoglycosides are complex, which poses a challenge for the efficient treatment of infectious diseases caused by these pathogens. To help clinicians treat infections more effectively, a more comprehensive understanding of antibiotic resistance mechanisms is urgently needed.

Methods: Plates were streaked to isolate bacteria from the intestinal contents of fish.

Psychotherapy with somatosensory stimulation as a complementary treatment for women with endometriosis-associated pain - a qualitative study.

Objective: This qualitative study aimed to explore patients' experiences with a novel treatment approach for endometriosis-associated pain, termed 'sinosomatics'. Specifically, it sought to understand women's experiences of the treatment and its components, the effects of the treatment on biological, psychological, and social levels, and how the women interpreted the changes they experienced.

Methods: We conducted ten semi-structured interviews with patients, who had undergone the complementary treatment for endometriosis-associated pain.

Greater Rotational Knee Laxity Observed at Second-Look Arthroscopy in Patients With Failed Meniscal Repair Performed at the Time of Anterior Cruciate Ligament Reconstruction.

Purpose: To evaluate the healing of meniscal repair performed concomitantly with anterior cruciate ligament reconstruction (ACLR) using second-look arthroscopy, as well as the relationship between meniscal healing and knee laxity using quantitative evaluation under anesthesia.

Methods: This retrospective study included patients who underwent primary double-bundle ACLR with meniscal repair between June 2016 and June 2021, with a 2-year minimum follow-up. Meniscal healing was evaluated by second-look arthroscopy at least 1 year postoperatively, and knee laxity was measured under general anesthesia preoperatively, intraoperatively, and at second-look arthroscopy.

The NADH-dependent flavin reductase ThdF follows an ordered sequential mechanism though crystal structures reveal two FAD molecules in the active site.

Two-component flavin-dependent monooxygenases are of great interest as biocatalysts for the production of pharmaceuticals and other relevant molecules, as they catalyze chemically important reactions such as hydroxylation, epoxidation and halogenation. The monooxygenase components require a separate flavin reductase, which provides the necessary reduced flavin cofactor. The tryptophan halogenase Thal from Streptomyces albogriseolus is a well-characterized two-component flavin-dependent halogenase.

Impact of 30 mL/kg fluid resuscitation completed within one hour on elderly septic shock patient.

Objective: This study aimed to investigate the prognostic impact of completing 30 mL/kg fluid resuscitation within 1 h in elderly septic shock patients.

Methods: This was a multicenter prospective observational cohort study. We applied logistic regression to assess the impact of completing 30 mL/kg fluid resuscitation within 1 h on respiratory support escalation including new-onset mechanical ventilation, bilevel positive airway pressure (BiPAP), and high-flow nasal cannula (HFNC) as well as heart failure (HF).

Optimizing regulatory frameworks for gene therapies in rare diseases: Challenges and solutions.

The advent of genetic medicines and advanced diagnostics has revolutionized the treatment landscape for rare diseases and, with over 10,000 identified conditions affecting millions globally, has the potential to improve many lives. Despite this progress, only 5% of rare diseases have FDA-approved therapies, highlighting a significant unmet need. This article examines the critical need for optimizing the regulatory environment to support the development and approval of gene therapies for rare and ultrarare diseases, which often face unique challenges due to their complexity in the midst of a rapidly evolving field.

Transcriptome Profiling, Cloning, and Characterization of AnGlu04478, a Ginsenoside Hydrolyzing β-Glucosidase from Aspergillus niger NG1306.

β-Glucosidase plays a pivotal role in transforming ginsenosides into specific minor ginsenosides. In this study, total ginsenosides from Panax notoginseng leaves were used as substrates to stimulate the growth of Aspergillus niger NG1306. Transcriptome analysis identified a β-glucosidase gene, Anglu04478 (1455 bp, 484 amino acids, 54.

Long-term effects of neoadjuvant chemotherapy in variant histology locally advanced colon cancer: a propensity score-matched analysis.

Purpose: Neoadjuvant chemotherapy (NAC) has proven valuable in treating locally advanced colon cancer (LACC) and is included as a treatment option for patients with clinical T4b colon cancer by the National Comprehensive Cancer Network. However, the long-term survival benefit of NAC in LACC remains debated, due to a lack of conclusive clinical trial results identifying the patients who would benefit most from NAC. This study aimed to assess the efficacy of NAC in patients with LACC based on histological subtype.

Comparison of Plasma p-tau217 and [F]FDG-PET for Identifying Alzheimer Disease in People With Early-Onset or Atypical Dementia.

Background And Objectives: To compare the diagnostic performance of an immunoassay for plasma concentrations of phosphorylated tau (p-tau) 217 with visual assessments of fluorine-18 fluorodeoxyglucose [F]FDG-PET in individuals who meet appropriate use criteria for Alzheimer dementia (AD) biomarker assessments.

Methods: We performed a retrospective analysis of individuals with early-onset (age <65 years at onset) and/or atypical dementia (features other than memory at onset), who were evaluated at a tertiary care memory clinic. All participants underwent measurements of CSF biomarkers (Aβ42, p-tau181, and total tau levels), as well as [F]FDG-PET scans, amyloid-PET scans, and plasma p-tau217 quantifications.

Dual Activities of Flower-Like Gold-Iron Oxide Nanozyme for Peroxidase-Mimicking and Glucose Detection.

Nanozymes, constituting of inorganic nanomaterials, are the sustainable and cost-effective alternatives of the naturally abundant enzymes. For more than a decade, nanozymes have shown astonishingly enhanced enzymatic activity as compared to its naturally occurring counterpart and emerged as a potential platform in biomedical science. The current study reports a novel flower shaped gold-iron oxide nanocomposite prepared via a facile and green solution phase redox mediated synthesis.

Machine learning in personalized laryngeal cancer management: insights into clinical characteristics, therapeutic options, and survival predictions.

Purpose: Over the last 40 years, there has been an unusual trend where, even though there are more varied treatments, survival rates have not improved much. Our study used survival analysis and machine learning (ML) to investigate this odd situation and to improve prediction methods for treating non-metastatic LSCC.

Methods: The surveillance, epidemiology and end results (SEER) database provided the data used for this study's analysis.

Expression of Recombinant Human α-Glucosidase in HEK293 Cells.

In mammals, intestinal α-glucosidase exists as a maltase-glucoamylase complex (MGAM) and a sucrase-isomaltase complex (SI). In this study, we transiently expressed human MGAM and SI in human embryonic kidney 293 (HEK293) cells. At pH 6.

A FRET assay to quantitate levels of the human β-cardiac myosin interacting heads motif based on its near-atomic resolution structure.

In cardiac muscle, many myosin molecules are in a resting or "OFF" state with their catalytic heads in a folded structure known as the interacting heads motif (IHM). Many mutations in the human β-cardiac myosin gene that cause hypertrophic cardiomyopathy (HCM) are thought to destabilize (decrease the population of) the IHM state. The effects of pathogenic mutations on the IHM structural state are often studied using indirect assays, including a single-ATP turnover assay that detects the super-relaxed (SRX) biochemical state of myosin functionally.

Pharmacokinetics/pharmacodynamics of glucocorticoids: modeling the glucocorticoid receptor dynamics and dose/response of commonly prescribed glucocorticoids.

Background And Purpose: The main features of the dynamics of the glucocorticoid receptor (GR) have been known for 50 years: 1) in the absence of glucocorticoid (G), the receptor is localized entirely in the cytoplasm; 2) upon G binding, GR is converted into a tightly bound G form and is rapidly imported into the nucleus where it can bind DNA and modulate transcription; 3) nuclear export of GR is very slow; and 4) the nuclear form of GR can recycle through an unbound form, back to the bound transcription modulating form without leaving the nucleus.

Experimental Approach: A kinetic model that captures these features is presented, a set of model parameters for dexamethasone is derived, and the clinical implication for the commonly used glucocorticoids is discussed.

Key Results: At the high concentrations normally used to describe G pharmacodynamics, the model reduces to the standard Michaelis-Menten equation with a that is a function of 4 model parameters.

The Hasson Versus Veress Trocar Wars: Determining the Safety Index of Laparoscopic Surgical Entry Techniques.

Background: Classically, there are two techniques for establishing pneumoperitoneum in laparoscopic and robotic surgeries: the closed Veress needle technique and Hasson's technique for open placement of laparoscopic ports. Most surgeons prefer the open Hasson technique, even though it is not the gold standard. Some surgeons still favour the Veress needle technique despite literature linking it to visceral and vascular damage.