To be or not to be phosphorylated: Understanding the role of Ebola virus nucleoprotein in the dynamic interplay with the transcriptional activator VP30 and the host phosphatase PP2A-B56.

Ebola virus (EBOV) transcription is essentially regulated via dynamic dephosphorylation of its viral transcription activator VP30 by the host phosphatase PP2A. The nucleoprotein NP has emerged as a third key player in the regulation of this process by recruiting both the regulatory subunit B56 of PP2A and its substrate VP30 to initiate VP30 dephosphorylation and hence viral transcription. Both binding sites are located in close proximity to each other in NP's C-terminal disordered region.

A pipeline for validation of Serendipita indica effector-like sRNA suggests cross-kingdom communication in the symbiosis with Arabidopsis.

Bidirectional communication between pathogenic microbes and their plant hosts via small (s)RNA-mediated cross-kingdom RNA interference (ckRNAi) is a key element for successful host colonisation. Whether mutualistic fungi of the Serendipitaceae family, known for their extremely broad host range, use sRNAs to colonize plant roots is still under debate. To address this question, we developed a pipeline to validate the accumulation, translocation, and activity of fungal sRNAs in post-transcriptional silencing of Arabidopsis thaliana genes.

Evaluating reproduction traits in a crossbreeding program between indigenous and exotic sheep in semi-arid lands.

Reproduction traits are important factors determining the efficiency of any sheep production system. This study evaluates the age at first lambing (AFL), lambing interval (LI), litter weight at birth (LBWT), litter weight at weaning (LWWT), birth weight of ewe (EBWT) and weaning weight of ewes (EWWT) in a crossbreeding program between the Red Maasai (RRRR) and Dorper sheep and their crosses, 75% Dorper and 50% Dorper (DDRR) breeds. All the traits significantly (P < 0.

Optimization of the cardiac delirium index by including age, decrease in butyrylcholinesterase actitivity, preoperative HbA1c, and postoperative hemoglobin levels: results of a secondary analysis of a prospective observational study.

Introduction: Postoperative delirium (POD) after cardiac surgery significantly affects the perioperative morbidity and mortality. Butyrylcholinesterase (BChE) is an enzyme primarily produced in the liver, which plays a crucial role in the hydrolysis of acetylcholine outside of neuronal synapses, referred to as extraneuronal hydrolysis. The integration of BChE activity into the cardiac delirium (CARDEL) index might increase its predictive power for identifying POD after cardiac surgery.

The older, the better: a comprehensive survey of soil organic carbon under commercial oil palm plantations.

Soil conditions of croplands are a frequent topic of scientific research. In contrast, less is known about large-scale commercial plantations of perennial crops such as oil palm. Oil palm is a globally important tropical commodity crop which contributes to both food and energy security due to its exceptional productivity.

Quantification of Different Types of Waste and Batteries Associated With the Widespread Usage of Continuous Glucose Monitoring Systems.

Background: People with diabetes benefit from continuous glucose monitoring (CGM) systems. A downside of these valuable aids for diabetes management is the generation of a tremendous amount of waste. This study aimed to quantify this CGM-related waste.

Microbiome of honey bee corbicular pollen: Factors influencing its structure and potential for studying pathogen transmission.

Honey bees are exposed to a diverse variety of microbes in the environment. Many studies have been carried out on the microbiome of bee gut, beebread, and flower pollen. However, little is known regarding the microbiome of fresh corbicular pollen, which can directly reflect microbes acquired from the environment.

Decoupling the Effects of Interface Chemical Degradation and Mechanical Cracking in Solid-State Batteries with Silicon Electrode.

Silicon is a promising negative electrode material for solid-state batteries (SSBs) due to its high specific capacity and ability to prevent lithium dendrite formation. However, SSBs with silicon electrodes currently suffer from poor cycling stability, despite chemical engineering efforts. This study investigates the cycling failure mechanism of composite Si/LiPSCl electrodes by decoupling the effects of interface chemical degradation and mechanical cracking.

Affective and social pain modulation in children-Experimental evidence using picture viewing.

Background: Children frequently encounter pain. Their pain like adults' pain is probably modulated by social-affective factors. Despite its clinical relevance, such pain modulation has not been explored experimentally in children, and little is known about specific factors accounting for it such as catastrophizing.

Toothbrushing behavior over time: A correlational analysis of repeatedly assessed brushing performance.

Effective toothbrushing is important for maintaining good oral health. Nonetheless, epidemiological data suggest that individuals often encounter challenges in achieving oral cleanliness when brushing their teeth. Various cross-sectional studies have observed brushing behavior of individuals and found some widespread behavioral deficits including neglect of inner surfaces and inconsistent brushing movements.

Association of aortic root diameter and vascular function with an exaggerated blood pressure response to exercise among elite athletes.

Background: The systolic blood pressure/workload (SBP/MET) slope was recently reported to reliably identify an exaggerated blood pressure response (eBPR) in the normal population and in athletes. We investigated whether the aortic root diameter (AoD) also correlates with an eBPR and vascular function in elite athletes.

Methods: We examined 652 healthy male elite athletes (age 25.

Central vs. Brachial Blood Pressure and Pulse Pressure Amplification for Mortality Risk Prediction in Patients Undergoing Coronary Angiography.

Background: Arterial hypertension is a significant risk factor for cardiovascular (CV) morbidity and mortality. Although central blood pressure (BP) evaluation is considered the gold standard, the reliability of non-invasive measurements remains unclear. Therefore, we compared the predictive value of invasively measured central BP with non-invasively measured brachial BP and analyzed pulse pressure (PP) amplification (delta-PP; difference between central and peripheral PP) as an independent predictor of mortality.

TEMPTED: time-informed dimensionality reduction for longitudinal microbiome studies.

Longitudinal studies are crucial for understanding complex microbiome dynamics and their link to health. We introduce TEMPoral TEnsor Decomposition (TEMPTED), a time-informed dimensionality reduction method for high-dimensional longitudinal data that treats time as a continuous variable, effectively characterizing temporal information and handling varying temporal sampling. TEMPTED captures key microbial dynamics, facilitates beta-diversity analysis, and enhances reproducibility by transferring learned representations to new data.

Excessive health behaviors in sports: links of orthorexia nervosa and exercise addiction with well-being, exercise activity in sports categories, and gender effects.

Background And Aims: Orthorexia nervosa (OrNe), the obsessive fixation on healthy eating, and exercise addiction (ExAdd) have been discussed as correlated excessive health behaviors with potential mental health implications. The role of gender-and sports-specific differences remains unclear. This study aimed to investigate the links of ExAdd, OrNe, and the non-pathological interest in healthy eating (healthy orthorexia; HeOr) with well-being and exercise activity in various sports, considering gender effects.

Abatacept Induces Long-Term Reconstitution of the B-Cell Niche in a Patient With CTLA-4 Haploinsufficiency: A Case Report.

Objectives: Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) haploinsufficiency is a rare genetic condition characterized by development of immune cytopenia, hypogammaglobulinemia, and/or lymphoproliferative disorder, as well as multiple autoimmunity. Treatment with abatacept was shown to alleviate autoimmune conditions, yet its long-lasting impact on bone marrow function remains undetermined.

Methods: We here present the case of a now 39-year-old woman with CTLA-4 haploinsufficiency with predominant CNS affection, yet multiorgan autoimmunity and lymphopenia.

Do sensorimotor insoles improve gait safety in patients with Parkinson's disease on a short scale?

Introduction: Parkinson's disease (PD) often leads to gait abnormalities, increasing the risk of falls and affecting daily life. Sensorimotor insoles aim to enhance foot sensitivity, potentially improving gait stability.

Methods: This study examined whether there are short-term effects of sensorimotor insoles on neural activation (measured by EEG), kinematic gait parameters (speed, cadence, step length, and step-length variability), and subjective gait stability in PD patients.

Genes, Gene Loci, and Their Impacts on the Immune System in the Development of Multiple Sclerosis: A Systematic Review.

Multiple sclerosis (MS) is a condition that is characterized by damage to the central nervous system (CNS) that causes patients to experience cognitive and physical difficulties. Although the disease has a complex etiology that involves genetic and environmental factors, little is known about the role of genes and gene loci in its development. Aims: This study aimed to investigate the effects of genes and gene loci on the immune system during the development of MS.

Silencing of the Gene via dsRNA Affects Root Development and Growth in the Invasive Weed .

("mile-a-minute" weed) is a global invasive alien weed that can cause severe damage to agroforestry ecosystems and significant agricultural losses worldwide. Although chemical, manual, or mechanical control methods are widely used to control , RNA interference (RNAi)-based biocontrol methods have rarely been reported for this species. The () gene, encoding an auxin response factor, plays an essential role in embryonic root initiation in .

New Antioxidant Caffeate Esters of Fatty Alcohols Identified in .

The stem bark of black locust ( L.) was extracted, and nine antioxidant compounds (-) were detected by high-performance thin-layer chromatography combined with the radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH•) assay, multi-detection, and heated electrospray high-resolution mass spectrometry. For structure elucidation, the methanolic crude extract was fractionated by solid-phase extraction, and the compounds were isolated by reversed-phase high-performance liquid chromatography with diode array detection.

Soluble Antigen (Ag)-Induced NETs Under Hypoxic Conditions Exert Cytotoxic Effects on Hepatic Cells In Vitro.

is a parasitic trematode that causes fasciolosis in sheep, provoking a decrease in their reproductive capacity, weight gain, meat and milk production, and wool quality. In the pathogenesis of , the penetration and migration of parasitic stages through the liver provoke intense inflammatory immune responses and tissue damage. The aim of this study was to investigate the cytotoxic effects of -induced ovine NETs in exposed hepatocytes in vitro, and to analyze whether antigens (Ag) trigger the release of ovine NETs under hypoxic conditions as well as the roles of matrix metalloproteinase-9 (MMP-9) and CD11b in this cellular process in vitro.

Toxoplasma gondii infection induces early host cell cycle arrest and DNA damage in primary human host cells by a MYR1-dependent mechanism.

Toxoplasma gondii, an obligate intracellular parasite, control its host cell cycle through mechanisms that are not fully understood. Key effector molecules, including MYR1 and HCE1, play roles in translocating parasite proteins and inducing host cellular cyclin E1 overexpression, respectively. We investigated the early role of MYR1- and HCE1-driven host cell cycle arrest and DNA damage (up to 3 h p.

Fire Reduces Soil Nitrate Retention While Increasing Soil Nitrogen Production and Loss Globally.

Elucidating the response of soil gross nitrogen (N) transformations to fires could improve our understanding of how fire affects N availability and loss. Yet, how internal soil gross N transformation rates respond to fires remains unexplored globally. Here, we investigate the general response of gross soil N transformations to fire and its consequences for N availability and loss.