Correlation between the sulfamethoxazole-trimethoprim resistance of Shigella flexneri and the sul genes.

The aim of this study was to discuss the correlation between the sulfamethoxazole-trimethoprim resistance of Shigella flexneri (S. flexneri) and the antibiotic resistance genes sul1, sul2, and sul3 and SXT element.From May 2013 to October 2018, 102 isolates of S.

Different mutations at position 562 of the hepatitis E virus capsid proteins exhibit differential effects on viral neutralizing activity.

The hepatitis E virus (HEV) capsid protein pORF2 comprises three potential N-linked glycosylation sites. One site, N562, is located at the cell attachment and neutralizing antigenic regions. The present study performed detailed analyses of the effects of specific amino acid substitutions at position 562 in the homodimerization, glycosylation, antigenicity, immunogenicity and neutralization activities of HEV pORF2.

Development and validation of a nomogram prognostic model for esophageal cancer patients with oligometastases.

Platinum-based chemotherapy is recommended as the standard treatment for metastatic esophageal cancer (EC) patients; however, the outcome is poor. Oligometastasis is less aggressive and has limited growth potential. However, the prognostic factors for EC patients with oligometastases was largely unknown.

Regorafenib and ginsenoside combination therapy: inhibition of HepG2 cell growth through modulating survivin and caspase-3 gene expression.

Background: This work aimed to investigate the inhibitory effect of regorafenib in combination with ginsenoside on the growth of HepG2 liver cancer cells.

Methods: HepG2 liver cancer cells were divided into blank control group, regorafenib single-drug group, ginsenoside single-drug group, and regorafenib/ginsenoside combination group. Cells in the regorafenib single-drug group were treated with regorafenib at 0.

Analysis of the laboratory indexes and risk factors in 189 cases of severe fever with thrombocytopenia syndrome.

The current study aimed to analyze the clinical characteristics of severe fever with thrombocytopenia syndrome (SFTS) and to explore the risk factors of critical patients. From 2016 to 2018, we collected the hospitalized diagnosed cases with SFTS in Jinan infectious disease hospital of Shandong University and analyzed by the descriptive epidemiological method. According to the prognosis, they were divided into general group and severe group.

Diagnostic Method of Liver Cirrhosis Based on MR Image Texture Feature Extraction and Classification Algorithm.

In order to improve the accuracy of cirrhosis staging diagnosis based on MR images, a diagnostic method combining image texture feature extraction and classification algorithm is proposed. Firstly, the liver MR image is preprocessed, the region of interest (ROI) image patch is extracted therefrom, and the ROI image is quantized and compressed by the Lloyd algorithm. Then, the ROI image is filtered by a local binary pattern (LBP) operator, and then the texture feature of a 20-dimensional gray-level co-occurrence Matrix (GLCM) in four directions on the LBP image is extracted.

MicroRNA-328-3p inhibits malignant progression of hepatocellular carcinoma by regulating MMP-9 level.

Objective: This study aims to explore the influence of microRNA-328-3p on proliferative and apoptotic abilities of hepatocellular carcinoma (HCC) cells, and the potential regulatory mechanisms.

Patients And Methods: Quantitative Real Time Polymerase Chain Reaction (qRT-PCR) was performed to detect the expression of microRNA-328-3p in 52 tumor tissue samples and paracancerous ones of HCC patients. The potential interaction between microRNA-328-3p level and clinical indicators of HCC patients was analyzed.

Resistance characteristics of CTX-M type in China.

The present study was to identify the drug resistance, resistance mechanism and the extended-spectrum β-lactamase (ESBLs) genotypes of () in Jinan. Susceptibility tests were performed by MIC-determination. The genotypes of β-lactamase were identified using PCR and DNA sequencing.

Study on the association between drug‑resistance and gene mutations of the active efflux pump acrAB‑tolC gene and its regulatory genes.

The aim of the present study was to investigate the correlation between the multi‑drug resistance of Shigella flexneri and the drug‑resistant gene cassette carried by integrons; in the meanwhile, to detect the associations between drug‑resistance and gene mutations of the active efflux pump acrAB‑tolC gene and its regulatory genes, including marOR, acrR and soxS. A total of 158 isolates were isolated from the stool samples of 1,026 children with diarrhoea aged 14 years old between May 2012 and October 2015 in Henan. The K‑B method was applied for the determination of drug resistance of Shigella flexneri, and polymerase chain reaction amplification was used for class 1, 2 and 3 integrase genes.

Protective effects of ursodeoxycholic acid against immune-mediated liver fibrosis in rats.

Background/aims: Ursodeoxycholic acid (UDCA), a natural component of bile, has been synthesized to treat cholestatic liver diseases such as primary biliary cirrhosis. Broad biochemical changes in UDCA-treated patients suggest beneficial effects of UDCA beyond stimulating hepatobiliary secretion and possible efficacy of the medicine in treating cirrhosis of other causes. The aim was to explore the potential benefit of UDCA in controlling immune-mediated hepatic fibrosis.