Fluorometric enhancement of the detection of HO using different organic substrates and a peroxidase-mimicking polyoxometalate.

Simple, sensitive and stable fluorometric sensors based on the polyoxotungstate intrinsic peroxidase (Na[α-SiWO]) induced fluorescent enhancement of benzoic acid (BA), thiamine (TH) and 3-(4-hydroxyphenyl)propionic acid (HPPA) for the detection of hydrogen peroxide (HO) are developed for the first time. In three assays, the three non-fluorescent substrates BA, TH and HPPA were oxidized with the ·OH radicals decomposed from HO under the catalysis of Na[α-SiWO] under basic pH conditions. The optimal conditions for the detection of HO were evaluated and possible mechanisms are also discussed.