AozC, a zn(II)Cys transcription factor, negatively regulates salt tolerance in Aspergillus oryzae by controlling fatty acid biosynthesis.

Background: In the soy sauce fermentation industry, Aspergillus oryzae (A. oryzae) plays an essential role and is frequently subjected to high salinity levels, which pose a significant osmotic stress. This environmental challenge necessitates the activation of stress response mechanisms within the fungus.

Enhanced Circularly Polarized Green Luminescence Metrics from New Enantiopure Binary -Pyrazolonate-Tb Complexes.

Achieving superior circularly polarized luminescence brightness () is an important subject and continuous challenge for chiroptical materials. Herein, by applying a binary molecular design for the synthesis of chiral organo-Tb molecules, a novel pair of mononuclear chiral -pyrazolate-Tb enantiomers, [Tb(PMIP)(,-Ph-PyBox)] () and [Tb(PMIP)(,-Ph-PyBox)] (), have been synthesized and characterized. The three 1-phenyl-3-methyl-4-(isobutyryl)-5-pyrazolone () ligands play the role of efficient luminescence sensitizers and strong light-harvesting antennas, while the enantiopure 2,6-bis(4-phenyl-2-oxazolin-2-yl) pyridine ligand (/) is employed as the strong point-chiral inducer.

Electrochemical Three-Component C-H Functionalization of Indoles with Sodium Bisulfite and Alcohols to Access Indole-Containing Sulfonate Esters.

Herein, an efficient electrochemical three-component C-H functionalization of indoles with sodium bisulfite and alcohols is described, providing a sustainable and convenient synthetic route for the construction of structurally valuable indole-containing sulfonate esters in moderate to good yields. This protocol proceeds in an undivided cell without any metal catalysts or oxidants, features a broad substrate scope, and has an excellent functional group tolerance. Preliminary mechanistic studies suggest that a radical-radical pathway may be involved in this three-component reaction system.

Alanine supplementation enhancing cordycepin production in Cordyceps militaris via upregulation of Cns2 and Cns3 genes expression levels.

Cordycepin, a key bioactive compound produced by Cordyceps militaris, faces the challenge of low productivity for commercial use. In this study, alanine supplementation in Cordyceps militaris boosted cordycepin production, peaking at 3 mg/g with 12 g/L concentration. Transcriptome analysis revealed 1711 differentially expressed genes, Pathway analysis indicates that protein processing in the endoplasmic reticulum was the most affected pathway.

Dynamic effects of ultrasonic treatment on flavor and metabolic pathway of pumpkin juice during storage based on GC-MS and GC-IMS.

In this study, the dynamic effects of ultrasonic treatment (0-400 W) on the volatile flavor compounds of pumpkin juice under different storage periods were investigated systematically using a combination of headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) and gas chromatography-ion mobility spectrometry (GC-IMS) techniques. A total of 139 and 46 volatile organic compounds (VOCs) were identified by GC-MS and GC-IMS, respectively. The results indicated that complex changes in volatile components occurred during storage.

PEDOTs-Based Conductive Hydrogels: Design, Fabrications, and Applications.

Conductive hydrogels combine the benefits of soft hydrogels with electrical conductivity and have gained significant attention over the past decade. These innovative materials, including poly(3,4-ethylenedioxythiophene) (PEDOTs)-based conductive hydrogels (P-CHs), are promising for flexible electronics and biological applications due to their tunable flexibility, biocompatibility, and hydrophilicity. Despite the recent advances, the intrinsic correlation between the design, fabrications, and applications of P-CHs has been mostly based on trial-and-error-based Edisonian approaches, significantly limiting their further development.

Paroxetine alleviates ulcerative colitis in mice via restoring intestinal microbiota homeostasis and metabolism.

Ulcerative colitis (UC) is an inflammatory bowel disease and psychological factors may be one of its pathogeneses. Selective serotonin reuptake inhibitor drug such as paroxetine with an effective anti-depression ability may be a new option for UC treatment. To evaluate the therapeutic effect of paroxetine on the exacerbation of UC symptoms caused by depression, a dual model of C57BL/6 mice was established using dextran sulphate sodium and chronic unpredictable mild stress (CUMS).

Research progress on the pathogenesis of psoriasis and its small molecule inhibitors.

Psoriasis is a prevalent chronic systemic immune disease characterized by T-cellmediated hyperproliferation of keratinized cells. Among its various manifestations, plaque-type psoriasis is the most common. Treatment options for psoriasis encompass topical medications, biological therapies, phototherapy techniques, and others.

Revealing 5-(3,5-difluorobenzyl)-1H-indazole as the active pharmacophore of ALK/ROS1 dual inhibitors through theoretical calculations and biological activity evaluations.

Anaplastic lymphoma kinase (ALK) and tyrosine protein kinase (ROS1) are recognized as driver genes in lung cancer, with dual inhibition of both targets offering a promising approach to enhance therapeutic outcomes in non-small cell lung cancer (NSCLC). Although numerous ALK/ROS1 inhibitors have received FDA approval, detailed research into the essential active structural motifs within these inhibitors remains limited. Addressing this gap, the current study employed computer-aided drug design (CADD) methodologies, incorporating bioisosteric and conformational similarity principles to design and synthesize 31 dual-target 2-morpholinobenzamide derivatives.

Microbial biosynthesis of nucleos(t)ide analogs: applications, and engineering optimization.

Nucleos(t)ide analogs constitute a diverse group of compounds derived from nucleosides and nucleotides, playing a crucial role in various biological processes. These analogs exhibit a wide range of applications, including their use as additives, antiviral, and anticancer agents, which makes them valuable in food and medical research. In this review, we will explore the applications of nucleos(t)ide analogs across different fields and discuss the latest advances in engineering and optimization strategies aimed at improving their production efficiency and tailoring their properties for specific purposes.

Targeting bacterial efflux pump effectively enhances the efficacy of Ru-based antibacterial agents against Gram-negative pathogen.

The rise of antibiotic resistance has posed a great threat to human's life, thus develop novel antibacterial agents is urgently needed. It worthies to noted that Ru-based antibacterial agents often showed robust potency against Gram-positive pathogens, disrupted bacterial membrane and avoided bacterial resistance, making they promising antibiotic candidates. However, they are generally less active when applied to negative pathogens.

Impact of catalyst support on water-assisted CO oxidation over PdO/MO (M = Sn, Ti, and Si) catalysts: experimental and theoretical investigation.

This study examines the impact of different supports, SnO, TiO, and SiO, on the catalytic performance and water resistance of PdO-based catalysts for CO oxidation. By merging experimental data with DFT calculations, we reveal the distinct characteristics exhibited by each catalyst. Specifically, PdO/TiO stands out with exceptional CO oxidation activity, attributed to its minute Pd grain size, robust CO adsorption capacity, and optimal Pd dispersion on the TiO surface.

Research progress and development strategy of PI3K inhibitors for breast cancer treatment: A review (2016-present).

Phosphatidylinositol 3-kinases (PI3Ks) are widely expressed in tissues and cells throughout the body and are involved in a variety of physiological processes including cell growth and metabolism. The phosphoinositide-3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of the rapamycin (mTOR) signaling pathway (PI3K/AKT/mTOR, PAM) is a promising target for the treatment of many cancer types because it is significantly linked to tumorigenesis and development. Aberrant activation of this pathway is observed in the majority of tumors, particularly in breast cancer.

An Overview of Naphthylimide as Specific Scaffold for New Drug Discovery.

Naphthylimides play a pivotal role as aromatic heterocyclic compounds, serving as the foundational structures for numerous pharmacologically significant drugs. These drugs encompass antibacterial, antifungal, anticancer, antimalarial, antiviral, anti-inflammatory, antithrombotic, and antiprotozoal agents. The planar and heteroaromatic characteristics of naphthylimides grant them a strong ability to intercalate into DNA.

Enhanced Flexible Piezoelectric Nanogenerators Using Ethanol-Exfoliated g-CN/PVDF Composites via 3D Printing for Self-Powered Applications.

This study presents the development of flexible piezoelectric nanogenerators (PENGs) utilizing graphitic carbon nitride (g-CN) nanoflakes (CNNFs) and polyvinylidene fluoride (PVDF) composites fabricated via the direct ink writing (DIW) 3D printing method. A novel approach of synthesizing CNNFs using the ethanol exfoliation method was demonstrated, which significantly reduces preparation time and cost compared to traditional acid exfoliation. The CNNFs are incorporated into PVDFs at varying weight percentages (5, 7.

AIE-based ruthenium complexes as photosensitizers for specifically photo-inactivate gram-positive bacteria.

The emergence of multidrug-resistant bacterial have caused severe burden for public health. Particularly, Staphylococcus aureus as one of ESKAPE pathogens have induced various infectious diseases and resulted in increasing deaths. Developing new antibacterial agents is still urgent and challenging.

Epigenetic Regulation of Fungal Secondary Metabolism.

Secondary metabolism is one of the important mechanisms by which fungi adapt to their living environment and promote survival and reproduction. Recent studies have shown that epigenetic regulation, such as DNA methylation, histone modifications, and non-coding RNAs, plays key roles in fungal secondary metabolism and affect fungal growth, survival, and pathogenicity. This review describes recent advances in the study of epigenetic regulation of fungal secondary metabolism.

[Construction of strains with organelles labeled with fluorescence and observation of organelle morphology].

The organelles in the multi-nucleated filamentous fungus present polymorphism. To observe the organelle morphology in . and provide references for the localization prediction of unknown proteins and the disclosure of biological reaction pathways in .

Characterization of the flavor profile of four major Chinese carps using HS-SPME-GC-MS combined with ultra-fasted gas chromatography-electronic nose.

The four major Chinese carps are highly popular for their distinctive nutritional benefits. However, the differences in flavor among these carps remain unclear. This study investigated the flavor profiles of these carps using headspace solid-phase micro-extraction gas chromatography-mass spectrometry (HS-SPME-GC/MS) combined with ultra-fasted gas chromatography electronic nose (GC E-nose).

The role and mechanisms of cordycepin in inhibiting cancer cells.

With the escalating incidence and mortality rates of cancer, there is an ever-growing emphasis on the research of anticancer drugs. Cordycepin, the primary nucleoside antibiotic isolated from Cordyceps militaris, has emerged as a remarkable agent for cancer prevention and treatment. Functioning as a natural targeted antitumor drug, cordycepin assumes an increasingly pivotal role in cancer therapy.

Fused in silico and bioactivity evaluation method for drug discovery: T001-10027877 was identified as an antiproliferative agent that targets EGFR and EGFR.

Background: Facing the significant challenge of overcoming drug resistance in cancer treatment, particularly resistance caused by mutations in epidermal growth factor receptor (EGFR), the aim of our study was to identify potent EGFR inhibitors effective against the mutant, a key player in resistance mechanisms.

Methods: Our integrated in silico approach harnessed machine learning, virtual screening, and activity evaluation techniques to screen 5105 compounds from three libraries, aiming to find candidates capable of overcoming the resistance conferred by the T790M and C797S mutations within EGFR. This methodical process narrowed the search down to six promising compounds for further examination.