Significantly enhanced substrate tolerance of Pseudomonas putida nitrilase via atmospheric and room temperature plasma and cell immobilization.

The objective of the study was to enhance the substrate tolerance of Pseudomonas putida nitrilase via atmospheric and room temperature plasma (ARTP) and cell immobilization. The mutant library was constructed by ARTP and rapidly screened by an OPA-TCA microscale reaction. A mutant strain of mut-D3 was obtained and its optimum substrate concentration was improved to 150mM from 100mM.

[Development of a genetic transformation system for Candida tropicalis based on a reusable selection marker of URA3 gene].

Candida tropicalis, a diploid asporogenic yeast, is frequently utilized in industrial applications and research studies. However, the low efficiency of genetic transformation limits the strain improvement by metabolic engineering. A reliable transformation and efficient deletion of target gene are prerequisite for molecular improvement of C.

[The use of ultrasensitive time-resolved fluoroimmunoassay for rapid detection of microcystin LR].

Objective: In order to provide a rapid and selectivity method for the determination of microcystin LR (MCLR).

Methods: An indirect competitive time-resolved fluoroimmunoassay (TRFIA) was developed. MC-LR-BSA was coated by physical adsorption onto the microtitre plate, MC-LR or sample with MC-LR as a competitor.

[Preparation and characterization of immunogen against microcystin LR].

Objective: To prepare Microcystins-LR (MC-LR) conjugates with the carrier protein for developing a new immune assay for MC-LR.

Methods: MC-LR was coupled to BSA or KLH by "activated ester" and "water-soluble carbodimide". Measure the combine ratio of MC-LR with KLH or BSA by the UV scanning or the Bradford's method.

Cyanide removal from wastewater using gas membranes: pilot-scale study.

Results are presented for the removal of cyanide from four different wastewaters using hollow-fiber gas membranes. The pilot-scale membrane facility had a maximum treatment capacity of 1000 L using 10 hollow-fiber modules with a total membrane surface area of 180 m2. The results are in general agreement with previous laboratory-scale studies.