Palladium-Catalyzed Dual C-H Carbonylation of Diarylamines Leading to Diversified Acridones under CO-Free Conditions.

A Pd-catalyzed dual C-H carbonylation of commercially available diarylamines using Co(CO) as a safe CO source has been developed. This methodology provides a facile approach for the synthesis of diversified acridones in moderate to good yields. The protocol features good functional group compatibility, operational safety, easy scale-up, and versatile transformations.

The structure and assembly mechanisms of T4-like cyanophages community in the South China Sea.

Marine ecosystems contain an immense diversity of phages, many of which infect cyanobacteria (cyanophage) that are largely responsible for primary productivity. To characterize the genetic diversity and biogeographic distribution of the marine T4-like cyanophage community in the northern South China Sea, the T4-like cyanophage portal protein gene () was amplified. Phylogenetic analysis revealed that marine T4-like cyanophages were highly diverse, with operational taxonomic units being affiliated with five defined clades (Clusters I-V).

Research on the environmental stability performance of chromite ore processing residue solidified products.

Chromite ore processing residue (COPR) is a hazardous waste because of leachable chromium, especially Cr(vi). Therefore, ascorbic acid (AA) and blast furnace slag (BFS) have been used to detoxify and solidify COPR. On this basis, environmental stability experiments with high temperature and freeze-thaw cycles were carried out to explore the stability performance of a solidified body with 40% COPR.

Synthesis of CoFeO@SiO-NH and its application in adsorption of trace lead.

In this work, an amine functionalized CoFeO magnetic nanocomposite material CoFeO@SiO-NH was prepared successfully by modifying coated-CoFeO@SiO magnetic nanoparticles with 3-aminopropyltriethoxysilane (APTES) and became an efficient adsorbent for the separation and analysis of trace lead in water. The CoFeO@SiO-NH magnetic nanoparticles were characterized using SEM, TEM, XRD, FTIR, VSM and BET techniques. Then, the adsorption mechanism was preliminarily investigated through ZETA, XPS, and adsorption kinetic experiments.

Characterization and genomic analysis of a novel Pseudoalteromonas phage PS_L5.

Pseudoalteromonas is a widely distributed bacterial genus that is associated with marine algae. However, there is still limited knowledge about their bacteriophage. In this study, we reported the isolation of a novel lytic bacteriophage that infects Pseudoalteromonas marina.

An improved ligand-assisted reprecipitation method to synthesize aqueous-phase CsPbBr perovskite nanocrystals and investigate their electrochemiluminescence behavior.

We propose a novel ligand-assisted reprecipitation method to synthesize aqueous-phase CsPbBr nanocrystals, the fluorescence intensity of which remained at 51% after 120 h. As a multifunctional additive, cesium trifluoroacetate (Cs-TFA) can improve the surface adsorption energy and induce nanocrystals to show significant anodic electrochemiluminescence (ECL) and stable cathodic ECL performances.

Thermal Analysis Kinetics Study of Pulverized Coal Combustion under Oxygen-Rich Atmosphere.

The thermal analysis kinetic behavior of pulverized coal combustion in different oxygen-rich atmospheres is studied with a thermogravimetric (TG) analyzer. The effects of heating rate on combustion characteristic are considered. The results showed that the combustion rate of pulverized coal increased and the burnout time decreased under the conditions of an oxygen-enriched atmosphere and high heating rate.

Isolation and genome sequencing of a novel lytic Pseudoalteromonas phage SL20.

Phage SL20, a novel lytic Pseudoalteromonas phage, was isolated from the coastal waters of the Yellow Sea, China. The microbiological characterization demonstrated that phage SL20 was relatively stable from 35 to 55 °C and the optimal pH was approximately 6.0.

Characterization, production optimization, and fructanogenic traits of levan in a new Microbacterium isolate.

Levan is a high-valued β-(2,6)-linked fructan with promising physicochemical and physiological properties and has diverse potential applications in the food, nutraceutical, pharmaceutical and cosmetic industry, but its commercial availability is still restricted to the relatively high costs of production. In this study, a strain identified as Microbacterium sp. XL1 was isolated from soil and highly produced exopolysaccharide (EPS).

Preparation and comparison of two medical dressings made from the collagens from fish and bovine.

Collagen is used in medical dressings because of its high hydrophilicity, low immunogenicity, excellent biocompatibility, and degradability. These features can promote cell proliferation and platelet agglomeration. Herein, we studied the preparation of gel dressing by using silver carp skin collagen and bovine collagen as raw materials.

Four previously undescribed diketopiperazines from marine fungus Aspergillus puniceus FAHY0085 and their effects on liver X receptor α.

Four previously undescribed diketopiperazine-type alkaloids including one oxepin-containing diketopiperazine-type alkaloid, oxepinamide L (1), three 4-quinazolinone alkaloids, puniceloids E-G (10-12), together with 12 known analogues, protuboxepin D (2), oxepinamides D-G, J-K and I (3-9), puniceloids B-D (13-15) and protubonine B (16), were isolated from the culture of the marine-derived fungus Aspergillus puniceus FAHY0085. The structures of the previously undescribed compounds were comprehensively elucidated by detailed interpretation of their NMR and HRESIMS data. Their absolute configurations were unambiguously determined by ROESY experiments, Marfey's method, calculated ECD experiments and single-crystal X-ray diffraction analysis.

Palladium-Catalyzed Carbonylative Dearomatization of Indoles to Achieve Carbonyl-Containing Spirocyclic Indolenines Bearing an All-Carbon Quaternary Center.

A Pd-catalyzed carbonylative dearomatization via an acyl Pd complex has been developed. Diversified carbonyl-containing spirocyclic indolenines with an all-carbon quaternary center were constructed in an efficient and straightforward way with good to excellent yields. The protocol features a simple catalytic system, operational simplicity, a broad substrate scope, easy scale-up, and versatile transformations.

The Screening of the Protective Antigens of Using the Reverse Vaccinology Approach: Potential Candidates for Subunit Vaccine Development.

The threat of bacterial septicemia caused by infection to aquaculture growth can be prevented through vaccination, but differences among strains may affect the effectiveness of non-conserved subunit vaccines or non-inactivated vaccines, making the identification and development of conserved antigens crucial. In this study, a bioinformatics analysis of 4268 protein sequences encoded by the J-1 strain whole genome was performed based on reverse vaccinology. The specific analysis included signal peptide prediction, transmembrane helical structure prediction, subcellular localization prediction, and antigenicity and adhesion evaluation, as well as interspecific and intraspecific homology comparison, thereby screening the 39 conserved proteins as candidate antigens for vaccine.

Palladium-Catalyzed Dearomatization of Indoles with Alkynes: Construction of Spirocyclohexaneindolenines.

A palladium-catalyzed dearomatization of indoles with alkynes has been developed, providing an efficient route to access a variety of synthetically useful spirocyclohexaneindolenines in moderate to good yields. The current method features a simple catalytic system, operational simplicity, and good functional group compatibility, which will contribute substantially to the development of dearomatization to access spiro compounds. Besides, the ubiquitous existence of spiro molecules, including spirocyclohexaneindolenines, in drugs and biological active molecules suggests the potential application of this methodology in medicinal chemistry.

Distribution of microplastics in different tissues of major commercial catches in different functional areas of the South Yellow Sea.

The microplastic (MP) pollution in different tissues of six commercial catches, including (Miichthys miiuy, Eleutheronema tetradactylum, Collichthys lucidus, Mugil cephalus, Portunus trituberculatus and Exopalaemon carinicauda) in Haizhou Bay (nori farming area), Yancheng offshore (wind power construction area) and the Nantong marine ranch area of the South Yellow Sea was investigated. MPs are ubiquitous in commercial catches; the average abundance of MPs is 5.19 ± 3.

Immunogenicity and protective efficacy of OmpA subunit vaccine against infection in : An effective alternative to the inactivated vaccine.

is a kind of zoonotic pathogen, which can cause bacterial septicemia in fish and bring huge economic losses to global aquaculture. Outer membrane proteins (Omps) are conserved antigens of , which can be developed as subunit vaccines. To evaluate the protective efficacy of inactivated vaccine and recombinant outer membrane protein A (OmpA) subunit vaccine against in juvenile , the present study investigated the immunogenicity and protective effects of both vaccines, as well as the non-specific and specific immune response of .

Study on the preparation and properties of acellular matrix from the skin of silver carp (Hypophthalmichthys molitrix).

Acellular matrices are mainly composed of mammalian tissues, and aquatic tissues with lower biological risks and less religious restrictions are considered alternatives to mammalian tissues. The acellular fish skin matrix (AFSM) has been commercially available. Silver carp has the advantages of farmability, high yield and low price, but there are few studies on the silver carp acellular fish skin matrix (SC-AFSM).

Purification efficiency of Pyropia-processing wastewater and microalgal biomass production by the combination of Chlorella sp. C2 cultivated at different culture temperatures and chitosan.

To elucidate the impacts of culture temperature on nutrient removal efficiency of Pyropia-processing wastewater (PPW) and microalgal biomass production, Chlorella sp. C2 was employed and cultivated in raw PPW under different temperatures. Results showed that, after incubating for 7 days, higher biomass (0.

Improving the thermostability of GH49 dextranase AoDex by site-directed mutagenesis.

As an indispensable enzyme for the hydrolysis of dextran, dextranase has been widely used in the fields of food and medicine. It should be noted that the weak thermostability of dextranase has become a restricted factor for industrial applications. This study aims to improve the thermostability of dextranase AoDex in glycoside hydrolase (GH) family 49 that derived from Arthrobacter oxydans KQ11.

Purification and characterization of cold-adapted and salt-tolerant dextranase from sp. THN1 and its potential application for treatment of dental plaque.

The cold-adapted and/or salt-tolerant enzymes from marine microorganisms were confirmed to be meritorious tools to enhance the efficiency of biocatalysis in industrial biotechnology. We purified and characterized a dextranase CeDex from the marine bacterium sp. THN1.

Enabling secure mutual authentication and storage checking in cloud-assisted IoT.

Internet of things (IoT) is a technology that can collect the data sensed by the devices for the further real-time services. Using the technique of cloud computing to assist IoT devices in data storing can eliminate the disadvantage of the constrained local storage and computing capability. However, the complex network environment makes cloud servers vulnerable to attacks, and adversaries pretend to be legal IoT clients trying to access the cloud server.

Discovery of a novel class of benzoxazole derivatives as histamine H receptor ligands for the treatment of neuropathic pain.

To discover effective analgesics, we summarize the synthesis, optimization, and pharmacological anti-nociceptive effects of a novel series of benzoxazole derivatives targeting H receptor (HR). The new benzoxazoles were assayed in vitro for histamine HR and HR binding affinity. The best compound 8d (2-methyl-6-(3-(4-methylpiperazin-1-yl)propoxy)benzo[d]oxazole) exhibited high affinity for HR (K = 19.

Physiological acclimation of Ulva prolifera to seasonal environmental factors drives green tides in the Yellow Sea.

To understand how seasonal factors could drive the formation of green tide blooms and their flotation and decay, we cultured the green tide algal species Ulva prolifera at various temperatures (5, 10, 15, 20, 25, 30, and 35 °C) and light intensities (40, 80, 140, 240, and 400 μmol photons m s). The results showed that the ratio of floating U. prolifera increased with increasing light and temperature, which was accompanied by morphological changes.

Surface Functionalized Magnetic Nanoparticles as a Selective Sorbent for Affinity Fishing of PPAR-γ Ligands from .

Coronary heart disease (CHD), which has developed into one of the major diseases, was reported to be treated by the target of peroxisome proliferators-activate receptor γ (PPAR-γ). As a natural medicine long used in the treatment of CHD, there are few studies on how to screen the target active compounds with high specific activity from . To advance the pace of research on target-specific active compounds in natural medicines, we have combined magnetic ligand fishing and functionalized nano-microspheres to investigate the active ingredients of PPAR-γ targets in .

Magnetic Ligand Fishing Using Immobilized Cyclooxygenase-2 for Identification and Screening of Anticoronary Heart Disease Ligands From .

Inhibition of cyclooxygenase-2 (COX-2) activity is an effective way for treatment of coronary heart disease. And as an important source of COX-2 inhibitors, bioactive compounds of and pharmacological mechanisms remained lacking in prospective researches. Therefore, for the purpose of accelerating the discovery of natural products targeting designed inhibitors, the COX-2 microreactor composed of functionalized microspheres and magnetic ligand fishing was developed and applied in , and the physicochemical properties of the COX-2 functionalized microspheres were characterized using Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometer (VSM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).