Novel Approach for Efficient Separation of Primary Amines Using Supercritical Fluid Chromatography.

Efficient enantioselective separation is a critical process in pharmaceutical and chemical industries for the production of chiral compounds. Herein, we developed a novel approach for the efficient enantioselective separation of primary amines using supercritical fluid chromatography (SFC) with a commercially available SFC column, Cel1. The key factors of separation, including cosolvent ratios, total cosolvent percentages, and temperature, were systematically assessed in this study.

Hydrophobic modification of starch via enzymatic grafting polymerization of fluorinated vinyl monomer.

The synthesis of starch grafted with poly (hexafluorobutyl methacrylate) (PHFBMA) was achieved using the horseradish peroxidase (HRP)/acetophenone (ACAC) green initiating system. The modified starch was characterized through FT-IR, F NMR, XPS and EDS to confirm the successful grafting of PHFBMA onto starch backbone. The SEM analysis revealed that the introduction of PHFBMA led to a partial disruption of the crystalline structure of starch, suggesting that PHFBMA did not undergo physical adsorption onto starch.

Tailoring industrial enzymes for thermostability and activity evolution by the machine learning-based iCASE strategy.

The pursuit of obtaining enzymes with high activity and stability remains a grail in enzyme evolution due to the stability-activity trade-off. Here, we develop an isothermal compressibility-assisted dynamic squeezing index perturbation engineering (iCASE) strategy to construct hierarchical modular networks for enzymes of varying complexity. Molecular mechanism analysis elucidates that the peak of adaptive evolution is reached through a structural response mechanism among variants.

Myceliophthora thermophila as promising fungal cell factories for industrial bioproduction: From rational design to industrial applications.

Myceliophthora thermophila stands out as a prominent fungal cell factory, garnering growing interest due to its distinctive traits advantageous. Currently, M. thermophila has been developed as an efficient cell factory, producing a variety of products from various raw materials.

Dissecting the anti-pancreatic cancer mechanism of gold nanorods mediate photothermal therapy through quantitative proteomics analysis.

Gold nanorods (GNRs) mediated photothermal therapy (PTT) represents a promising technique for cancer treatment, utilizing GNRs in conjunction with near-infrared (NIR) laser irradiation to convert energy into heat. In the present study, we employed PTT to induce apoptosis in pancreatic cancer cells and investigated its underlying mechanisms through quantitative proteomics analysis. Initially, we established that temperatures ranging from 47 to 51°C significantly enhance cellular apoptosis without inducing necrosis.

Hapten prediction, monoclonal antibody preparation, and development of an immunochromatographic assay for the detection of fenamiphos.

Fenamiphos (FENA) is an organophosphorus insecticide, and its residues in fruits, vegetables, and the environment have raised concerns. Therefore, it is very important to develop a simple, rapid, and accurate method for FENA detection. In this study, a novel FENA hapten was designed and predicted based on computer-aided simulation technology, and high-performance anti-FENA monoclonal antibodies were screened using a matrix effect-enhanced screening method, with a half-maximal inhibitory concentration of 1.

Trehalose Inhibits ferroptosis Through Activating SIRT3/SOD2 Signaling Axis and Alleviates Brain Injury After Traumatic Brain Injury.

Trehalose has neuroprotective effects in neurodegenerative diseases. This study aimed to explore the impact of trehalose on traumatic brain injury (TBI) by investigating its role in neuroprotection. The TBI mice model was established utilizing the cortical impact technique followed by trehalose treatment.

A cross-species inducible system for enhanced protein expression and multiplexed metabolic pathway fine-tuning in bacteria.

Inducible systems are crucial to metabolic engineering and synthetic biology, enabling organisms that function as biosensors and produce valuable compounds. However, almost all inducible systems are strain-specific, limiting comparative analyses and applications across strains rapidly. This study designed and presented a robust workflow for developing the cross-species inducible system.

Anomalous Node Detection in Blockchain Networks Based on Graph Neural Networks.

With the rapid development of blockchain technology, fraudulent activities have significantly increased, posing a major threat to the personal assets of blockchain users. The blockchain transaction network formed during user transactions can be represented as a graph consisting of nodes and edges, making it suitable for a graph data structure. Fraudulent nodes in the transaction network are referred to as anomalous nodes.

Correlation Study Between Dietary Behaviors, Lifestyle, and Psychological Problems in Chinese Children Aged 3-7.

Objective: This study aimed to assess the psychological health status of children aged 3-7 years in Wuxi and analyze the correlation between dietary behaviors, lifestyle, and psychological problems.

Methods: Using a stratified cluster random sampling method, 3-7-year-old children from 18 kindergartens across Wuxi were selected as the survey subjects. An online survey was conducted to collect demographic information about children and their parents, dietary information, lifestyle data, and family backgrounds.

Evaluation of Stress Tolerance and Fermentation Performance in Commercial Yeast Strains for Industrial Applications.

This study evaluates the stress tolerance and metabolic adaptability of twelve yeast strains, including eleven commercial strains from Wyeast Laboratories and one prototrophic laboratory strain, under industrially relevant conditions. Yeast strains were assessed for their fermentation performance and stress responses under glucose limitation, osmotic stress, acid stress, elevated ethanol concentrations, and temperature fluctuations. Results revealed significant variability in glucose consumption, ethanol production, and stress tolerance across strains.

The Electrochemical Detection of Bisphenol A and Catechol in Red Wine.

The use of nanozymes for electrochemical detection in the food industry is an intriguing area of research. In this study, we synthesized a laccase mimicking the MnO@CeO nanozyme using a simple hydrothermal method, which was characterized by modern analytical methods, such as transmission electron microscope (TEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX), etc. We found that the addition of MnO significantly increased the laccase-like activity by 300% compared to CeO nanorods.

Effect of Whey Protein Changes on Milk Flavor and Sensory Characteristics During Heating.

The flavor of dairy products crucially affects consumer purchase preference. Although the flavor and sensory perception of milk can be influenced by heat treatment during processing, the exact mechanism remains unclear. Therefore, this study analyzed the whey protein content and structural changes of milk heated at different time and temperature combinations and evaluated the flavor compounds and sensory characteristics of milk.

Variations in the Impact of Gingerols' Conversion to Shogaols on the Properties of Corn Starch with Different Amylose Contents.

The polyphenol-starch complex has become a hot research topic since it is evident that this modification method can alter the physicochemical properties of starch as well as improve its nutritional value. This work aimed to evaluate the effect of ginger polyphenol gingerols (GNs) and shogaols (SNs) on the structure of starch with different amylose content (WCS, CS, G56, G80). Textural and rheological results indicated that GNs and SNs had more pronounced inhibitory retrogradation effects for relative low-level amylose starches (WCS and CS) compared to relative high-level amylose starches (G56 and G80).

Influence of Phosphate Marinades on the Quality and Flavor Characteristics of Prepared Beef.

Phosphate has been widely used in beef to improve processing characteristics such as tenderness and water-holding capacity. However, the effects of phosphates on the quality and especially the flavor of beef are not well understood. This study investigated the influence of eight different phosphate marinade solutions on the quality and flavor of prepared beef.

Preparation of ofloxacin molecularly imprinted polymer Raman sensor based on magnetic graphene oxide.

Ofloxacin is a commonly used quinolone antibiotic that is also used as a feed supplement in livestock production and in plant disease prevention and treatment. However, the excessive use and abuse of ofloxacin will accumulate along the food chain and endanger human health. Therefore, the development of a simple, rapid, and sensitive detection method for the determination of ofloxacin is critical.

Gut microbial-derived phenylacetylglutamine accelerates host cellular senescence.

Gut microbiota plays a crucial role in the host health in the aging process. However, the mechanisms for how gut microbiota triggers cellular senescence and the consequent impact on human aging remain enigmatic. Here we show that phenylacetylglutamine (PAGln), a metabolite linked to gut microbiota, drives host cellular senescence.

Anti-CRISPR proteins in Gluconobacter oxydans inactivate FnCas12a by acetylation.

Gluconobacter oxydans is an important chassis cell for one-step production of vitamin C. Previous studies reported that CRISPR/Cas12a is naturally inactivated in G. oxydans, but the specific mechanism remains unclear.

A flexible wearable sensor based on the multiple interaction and synergistic effect of the hydrogel components with anti-freezing, low swelling for human motion detection and underwater communication.

To meet the increasing demand for wearable sensor in special environment such as low temperature or underwater, a multifunctional ionic conducting hydrogel (Gel/PSAA-Al hydrogel) with anti-freezing and low swelling for human motion detection and underwater communication was prepared using gelatin (Gel), [2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide (SBMA), acrylamide (AAm), acrylic acid (AAc), and AlCl. Due to reversible hydrogen bonding, electrostatic interactions and metal coordination crosslinking between the polymer networks, the resulting Gel/PSAA-Al hydrogels present low swelling property in water and exhibit large tensile properties (~1050 %), high tensile strength (~250 kPa) and excellent fatigue resistance. In addition, the hydration capacity of SBMA and AlCl endows the Gel/PSAA-Al hydrogel fantastic anti-freezing (-31.

P4HA3 depletion induces ferroptosis and inhibits colorectal cancer growth by stabilizing ACSL4 mRNA.

Colorectal cancer (CRC) is a malignancy with high global incidence and mortality rates, posing a serious threat to human health. Despite favorable outcomes following early detection and surgical intervention, the asymptomatic nature of CRC often results in delayed diagnoses, limiting surgical treatment options. Furthermore, effective therapeutic drugs for CRC remain lacking in clinical practice, highlighting an urgent need to identify novel therapeutic targets.

Indoleamine 2, 3-dioxygenase 1 inhibition mediates the therapeutic effects in Parkinson's disease mice by modulating inflammation and neurogenesis in a gut microbiota dependent manner.

Abnormal tryptophan metabolism is closely linked with neurological disorders. Research has shown that indoleamine 2,3-dioxygenase 1 (IDO-1), the first rate-limiting enzyme in tryptophan degradation, is upregulated in Parkinson's disease (PD). However, the precise role of IDO-1 in PD pathogenesis remains elusive.

Flow-Electrode capacitive deionization for enhanced selective separation of Ammonia, Phosphorus, and caproate from sewage sludge fermentation: Performance and mechanistic insights.

Caproic acid has broad applications and can be produced from activated sludge via fermentation, but its quality is hindered by ammonia (NH-N) and reactive phosphorus (RP) in the fermentation broth. However, flow-electrode capacitive deionization (FCDI), a novel ion separation technology that operates continuously without secondary pollution seems to be an efficient process that separates the ions. The results showed that at pH 5.

Recombinant collagen microneedles for transdermal delivery of antibacterial copper-DNA nanoparticles to treat skin and soft tissue infections.

Skin and soft tissue infections (SSTI) include bacterial infections of the skin, muscles, and connective tissue such as ligaments and tendons. SSTI in patients with immunocompromising diseases may lead to chronic, hard-to-heal infected wounds, resulting in disability, amputation, or even death. To treat SSTI and rebuild the defensive barrier of the skin, here we utilize recombinant type XVII collagen protein (rCol XVII) to construct biodegradable, regenerative collagen microneedles (rCol-MNs) for transdermal delivery of antibacterial agents.

Characteristics of adverse events and clinical risks of Lecanemab based on FAERS data.

Objective: This study aims to analyze the distribution of adverse events (AEs) related to Lecanemab in real-world settings based on FAERS database data.

Methods: Using the FAERS database, AE data related to Lecanemab was collected from Q3 2023 to Q2 2024. Signal mining was conducted using frequency and Bayesian methods to identify positive signals associated with Lecanemab.