Identification and functional characterization of AsWRKY9, a WRKY transcription factor modulating alliin biosynthesis in garlic (Allium sativum L.).

Background: The variations in alliin content are a crucial criterion for evaluating garlic quality and is the sole precursor for allicin biosynthesis, which is significant for the growth, development, and stress response of garlic. WRKY transcription factors are essential for enhancing stress resistance by regulating the synthesis of plant secondary metabolites. However, the molecular mechanisms regulating alliin biosynthesis remain unexplored.

Simultaneous sensitive detection and imaging of dual microRNAs through DNA tetrahedral scaffold-corbelled autonomous-motion molecular machine.

Sensitive and accurate detection and imaging of different microRNAs (miRNAs) in cancer cells hold great promise for early disease diagnosis. Herein, a DNA tetrahedral scaffold (DTS)-corbelled autonomous-motion (AM) molecular machine based fluorescent sensing platform was designed for simultaneous detection of two types of miRNAs (miRNA-21 and miRNA-155) in HeLa cells. Locking-strand-silenced DNAzymes (P:L duplex) were firstly grafted at the loop of target-analogue-embedded double-stem hairpin substrates (TDHS) of DTS, making the sensor in a "signal off" state due to the closely distance between modified fluorophores (FAM and Cy5) with the corresponding quenchers (BHQ1 and BHQ2).

Synergistic Binding Sites in a Robust and Scalable Metal-Organic Framework for Record CH Capture.

Effectual CH reclamation from CH/N blends by existing physisorbents in industrialization confronts the adversity of frustrated separation performance, weak structural strength, and restricted scale-up preparation. To solve aforesaid bottlenecks, herein, a strategy is presented to fabricate synergistic strong recognition binding sites in a robust and scalable optimum Cu(pma) with ultramicroporous feature regarding superb CH separation versus N. By virtue of the synergistic contribution of multiple affinities accompanied by enormous potential field overlap of pore restriction, it imparts strong recognition binding toward CH molecules.

Single-Atom-Induced Hybridization States Promote the Direct Trapping of Hot Carriers by Reactants for Photocatalysis.

Single-atom manipulation has emerged as an effective strategy for enhancing the photocatalytic efficiency. However, the mechanism of photogenerated carrier dynamics under single-atom modulation remains unclear. Combining first-principles calculations and non-adiabatic molecular dynamics simulations, we systematically studied carrier transfer and recombination in the oxygen reduction reaction of single-atom-doped CN systems.

Soliton and rogue wave excitations in the Chen-Lee-Liu derivative nonlinear Schrödinger equation with two complex PT-symmetric potentials.

We demonstrate that fundamental nonlinear localized modes can exist in the Chen-Lee-Liu equation modified by several parity-time (PT) symmetric complex potentials. The explicit formula of analytical solitons is derived from the physically interesting Scarf-II potential, and families of spatial solitons in internal modes are numerically captured under the optical lattice potential. By the spectral analysis of linear stability, we observe that these bright solitons can remain stable across a broad scope of potential parameters, despite the breaking of the corresponding linear PT-symmetric phases.

g-CN S-Scheme Homojunction through Van der Waals Interface Regulation by Intrinsic Polymerization Tailoring for Enhanced Photocatalytic H Evolution and CO Reduction.

The effective S-scheme homojunction relies on the precise regulation of band structure and construction of advantaged charge migration interfaces. Here, the electronic structural properties of g-CN were modulated through meticulous polymerization of self-assembled supramolecular precursors. Experimental and DFT results indicate that both the intrinsic bandgap and surface electronic characteristics were adjusted, leading to the formation of an in-situ reconstructed homojunction interface facilitated by intrinsic van der Waals forces.

Tröger's Base as a Potential Bridge to Type-I Photosensitizers: Mechanism and Antitumor Applications.

In contrast to Type-II photodynamic therapy (PDT), Type-I PDT with less oxygen consumption has shown great potential against tumor hypoxia. However, there are limited strategies available for designing Type-I photosensitizers (PSs). Herein, we present a promising strategy for synthesizing Type-I PSs () using Tröger's base (TB) framework.

Effect of Warming on Soil Fungal Community Along Altitude Gradients in a Subalpine Meadow.

The subalpine grassland ecosystem is sensitive to climatic changes. Previous studies investigated the effects of warming on grassland ecosystems at a single altitude, with little information about the response of subalpine meadows to warming along altitude gradients. This study aimed to evaluate the effects of warming on aboveground grass, belowground soil properties, and fungal community along altitude gradients in the subalpine meadow of Mount Wutai using the high-throughput sequencing method.

Pd(OAc)-Catalyzed Approach to Phenanthridin-6(5)-one Skeletons.

Herein, we present a Pd(OAc)/Dppp-catalyzed synthesis of 4-arylphenanthridinones from 2-bromobenzamides and iodobenzene, which undergoes successive Ullman cross-coupling, C-H activation, and oxidative coupling dehydrogenation process. The presented methods offer an adaptable and modular synthesis route for efficiently producing a wide array of valuable phenanthridiones, demonstrating exceptional compatibility with functional groups. Alternatively, a 1:1 cross-coupling reaction utilizing an intramolecular norbornene moiety as the ligand resulted in phenanthridinones through -arylation and C-H activation.

Energy-Transfer-Based Dual-Mode PEC-ECL Biosensor for Acetamiprid Analysis Sensitized by Two-Step DNA Circuit Amplification.

Sensitive and accurate determination of acetamiprid is highly desirable for guaranteeing food safety. In this Letter, an energy-transfer-based dual-mode biosensor was developed using zinc-based metal-organic frameworks (Zn-MOFs) acting as both photoelectrochemical (PEC) and electrochemiluminescent (ECL) donors and Pt@CuO cubic nanocrystals (CNs) as the energy acceptor for detecting acetamiprid. By integration of aptamer recognition with two-step DNA circuit amplification (entropy-driven DNA cycle and DNA walker), the detection of acetamiprid was converted into the assay of abundant intermediate DNA strands.

Effects of functional training on physical and technical performance among the athletic population: a systematic review and narrative synthesis.

Background: The evidence indicates that functional training is beneficial for athletes' physical and technical performance. However, a systematic review of the effects of functional training on athletes' physical and technical performance is lacking. Therefore, this study uses a literature synthesis approach to evaluate the impact of functional training on the physical and technical performance of the athletic population and to extend and deepen the existing body of knowledge.

Nanoparticle-mediated light-driven LAMP combined with test strips for sensitive and rapid visual detection of antibiotic resistance genes.

Antibiotic resistance genes (ARGs) are markers of drug-resistant pathogens, monitoring them contributes to prevent resistance to drugs. The detection methods for ARGs including PCR and isothermal amplification are sensitive and selective. However, it may take several hours or cannot be used on spot.

Electrochemical Self-Sacrificial Label Conversion Coupled with DNA Framework Nanomachine Mediated Serotonin Sensing with Highly Minimized Background Noise.

Conventional solid/liquid electrochemical interfaces typically encounter challenges with impeded mass transport for poor electrochemical quantification due to the intricate pathways of reactants from the bulk solution. To address this issue, this work reports an innovative approach integrating a target-activated DNA framework nanomachine with electrochemically driven metal-organic framework (MOF) conversion for self-sacrificial biosensing. The presence of the target biomarker serotonin initiates the DNA framework nanomachine by an entropy-driven circuit to form a cross-linked nanostructure and subsequently release the Fe-MOF probe.

Pd(OAc)-Catalyzed Synthesis of Imidazo[1,2-]phenanthridines via a Sequential Ullmann Coupling and Oxidative Coupling Dehydrogenation.

The Ullmann coupling and oxidative coupling dehydrogenation reactions have occurred sequentially, catalyzed by Pd(OAc), which unexpectedly yielded fused imidazo[1,2-]phenanthridine derivatives in good to high yields. Structural analysis of the intermediate and final products indicated that the protocol did not include C-H and N-H arylation.

Insertional mutagenesis of AIDA or CYP720B1 in the green alga Chlamydomonas reinhardtii confers copper(II) tolerance and increased biomass.

The widespread use of copper (Cu) in industrial and agricultural settings leads to the accumulation of excess Cu within aquatic ecosystems, posing a threat to organism health. Microalgal bioremediation has emerged as a popular and promising solution to mitigate the risks. Nevertheless, the genetic underpinnings and engineering tactics involved in heavy metal bioremediation by microalgae remain inadequately elucidated.

Mapping the structure of biomarkers in autism spectrum disorder: a review of the most influential studies.

Background: Autism spectrum disorder is a distinctive developmental condition which is caused by an interaction between genetic vulnerability and environmental factors. Biomarkers play a crucial role in understanding disease characteristics for diagnosis, prognosis, and treatment. This study employs bibliometric analysis to identify and review the 100 top-cited articles' characteristics, current research hotspots and future directions of autism biomarkers.

PTGES3 proteolysis using the liposomal peptide-PROTAC approach.

Background: Hepatocellular carcinoma (HCC) is the leading cause of cancer-related deaths worldwide, and the lack of effective biomarkers for early detection leads to poor therapeutic outcomes. Prostaglandin E Synthase 3 (PTGES3) is a putative prognostic marker in many solid tumors; however, its expression and biological functions in HCC have not been determined. The proteolysis-targeting chimera (PROTAC) is an established technology for targeted protein degradation.

Overexpression of gene increases the rice resistance to pv. through negatively regulating transcription activator-like effectors translocation.

Bacterial leaf blight (BLB) caused by pv. () has shown a high incidence rate in rice fields in recent years. Rice resistance breeding is considered as the most effective method for achieving economical and sustainable management of BLB disease.

RNA modifications in plant adaptation to abiotic stresses.

Epitranscriptomic chemical modifications of RNAs have emerged as potent regulatory mechanisms in the process of plant stress adaptation. Currently, over 170 distinct chemical modifications have been identified in mRNAs, tRNAs, rRNAs, microRNAs (miRNAs), and long noncoding RNAs (lncRNAs). Genetic and molecular studies have identified the genes responsible for addition and removal of chemical modifications from RNA molecules, which are known as "writers" and "erasers," respectively.

Effects of two amino acid transporter-like genes on potato growth.

Amino acid transporters are membrane proteins that mediate amino acid transport across the plasma membrane. They play a significant role in plant growth and development. The amino acid permease (AAP) subfamily belongs to the activating transcription factor family, which is one of the main amino acid transporter families.

Orthographic-phonological mapping impairments in Chinese children with developmental dyslexia: insights from an ERP investigation.

In the realm of logographic writing systems, such as Chinese characters, orthographic transparency fundamentally differs from alphabetic languages, posing unique challenges for individuals with developmental dyslexia (DD). This study employed event-related potentials (ERPs) and a masked priming paradigm to investigate how Chinese children with DD compared to typically developing (TD) children in their utilization of orthographic-phonological mapping rules during the processing of pseudocharacters. The findings revealed noteworthy distinctions between TD and DD children.

Identification of WRKY transcription factors in Ipomoea pes-caprae and functional role of IpWRKY16 in sweet potato salt stress response.

Background: WRKY transcription factors are plant-specific and play essential roles in growth, development, and stress responses, including reactions to salt, drought, and cold. Despite their significance, the WRKY genes in the wild sweet potato ancestor, Ipomoea pes-caprae, remain unexplored.

Results: In this study, 65 WRKY genes were identified in the I.

Photothermally-enhanced ferroptotic-chemo therapy enabled by ZIF-derived multizyme.

A multi-functional single-Fe-atom nanozyme (Fe-SAzyme) is designed, integrating the near-infrared photothermal property, the ability to carry chemoagent (doxorubicin - DOX), and nanocatalytic activities mimicking peroxidase, oxidase, and glutathione oxidase. The nanocatalytic activities act cooperatively to effectively produce cytotoxic radicals in the tumor microenvironment (TME), thereby leading to ferroptosis of cancer cells. The photothermal effect not only enhances the nanocatalytic therapy but also enables photothermal therapy.

Prediction of high- superconductivity in HSX below megabar pressure.

The discovery of high critical temperature in cubic HS under high pressures attracts significant attention. Of particular recent interest is to search for superconductors stabilized at low pressure in order to approach practical applications. To examine the doping effect on the stability and superconductivity of HS, we construct HSX by replacing half of the S atoms with an X atom (X = Si, Ge, Sn, P, As, Sb, Te, Cl, Br and I).

Comprehensive characterization of nutritional components in sweetpotato (Ipomoea batatas [L]. Lam.) during long-term post-harvest storage.

To uncover the variation patterns of the nutritional components in sweetpotato storage roots during long-term storage comprehensively, the general nutrients, phytochemicals, and starch properties of nine sweetpotato varieties with different flesh colors were quantified and analyzed by chemical and physical techniques. During the storage, the starch content decreased firstly and then increased, with sugar content the opposite. The crude protein content and the total dietary fiber content both increased continuously.