Preparation and Characterization of a Novel Magnetic Molecularly Imprinted Polymer Capable of Isolating and Purifying Cordycepin from a Submerged Culture of the Caterpillar Medicinal Mushroom Cordyceps militaris (Ascomycetes).

In this work, magnetic molecularly imprinted polymer (MMIP) capable of selectively recognizing and adsorbing cordycepin was prepared. The MMIP was prepared using cordycepin as the template molecule, methacrylic acid and acrylamide as the functional monomer and ethylene glycol dimethacrylate as the crosslinker. The MMIP was analyzed using various techniques including transmission electron microscopy, thermogravimetric analysis, Fourier transform infrared spectroscopy, vibrating sample magnetometer and x-ray diffraction.

The Artist's Conk Medicinal Mushroom Ganoderma applanatum (Agaricomycetes): Mycological, Mycochemical, and Pharmacological Properties: A Review.

As a commonly used Chinese herbal medicine, Ganoderma applanatum (Pers.) Pat., also known as flat-ling Ganoderma (Chinese name bianlingzhi), old mother fungus (laomujun), and old ox liver (laoniugan), has high medicinal value.

Ganoderic Acid A: A Potential Natural Neuroprotective Agent for Neurological Disorders: A Review.

Ganoderic acid A (GAA) is one of the major triterpenoids in Ganoderma lucidum (GL). Accumulating evidence has indicated that GAA demonstrates multiple pharmacological effects and exhibits treatment potential for various neurological disorders. Here, the effects and mechanisms of GAA in the treatment of neurological disorders were evaluated and discussed through previous research results.

Analysis of surgical site infection and tumour-specific survival rate in patients with renal cell carcinoma after laparoscopic radical nephrectomy.

Surgical site infections (SSIs) may pose a significant risk to patients undergoing surgery. This study aims to explore the risk factors for SSIs in patients undergoing laparoscopic radical nephrectomy for renal cell carcinoma and the impact of infection on tumour-specific survival (CSS) after nephrectomy for renal cell carcinoma. To explore the risk factors for SSIs in patients undergoing laparoscopic radical nephrectomy for renal cell carcinoma and the impact of infection on tumour-specific survival (CSS) after nephrectomy for renal cell carcinoma.

GSG2 facilitates the progression of human breast cancer through MDM2-mediated ubiquitination of E2F1.

Background: Breast cancer (BC) has posed a great threat to world health as the leading cause of cancer death among women. Previous evidence demonstrated that germ cell-specific gene 2 (GSG2) was involved in the regulation of multiple cancers. Thus, the clinical value, biological function and underlying mechanism of GSG2 in BC were investigated in this study.

miR-4739/ITGA10/PI3K signaling regulates differentiation and apoptosis of osteoblast.

Introduction: To probe the impacts and biological roles of miR-4739/ITGA10 on the proliferation, differentiation and apoptosis of osteoblasts.

Methods: Bioinformatics analysis was conducted to screen the key genes in osteoporosis. The upstream miRNAs of ITGA10 were predicted by TargetScan.

Hypoxic Breast Cancer Cell-Derived Exosomal SNHG1 Promotes Breast Cancer Growth and Angiogenesis via Regulating miR-216b-5p/JAK2 Axis.

Background: Hypoxia is an important process that involved in the tumor microenvironment. In addition, hypoxic tumor cell-derived exosomes could promote tumor growth and angiogenesis. Thus, we aimed to investigate whether exosomes could regulate tumor development and progression under hypoxia in breast cancer.

Long Noncoding RNA NR2F1-AS1 Enhances the Migration and Invasion of Hepatocellular Carcinoma via Modulating miR-642a/DEK Pathway.

Purpose: Hepatocellular carcinoma (HCC), a malignant tumor that exists worldwide, has a high morbidity and mortality rate. Previous studies have reported that lncRNA NR2F1-AS1 plays a critical role in several cancers. Here, we aimed to investigate the biological function of NR2F1-AS1 and its molecular mechanism in the migration and invasion of HCC.

Bisphenol A exposure prenatally delays bone development and bone mass accumulation in female rat offspring via the ERβ/HDAC5/TGFβ signaling pathway.

Previous studies have suggested that bisphenol A (BPA) has a toxic effect on bone development; however, its pathological mechanism has not been fully elucidated. In the present study, pregnant Wistar rats were intragastrically administered BPA (10 μg/kg per day) during gestational days 14-21. Then, bone tissues were obtained from neonatal rats on postnatal day 1 for histological analysis, and the bone mass of adult rat offspring was analyzed by micro-CT at postnatal week 10.

Expression of miR-614 in gastric cancer and its effect on invasion and proliferation of gastric cancer cells HGC-27.

Purpose: To investigate the expression of miR-614 in serum of gastric cancer patients and its effect on invasion and proliferation of gastric cancer cell line HGC-27.

Methods: Thirty patients with gastric cancer from May 2016 to November 2018 comprised the research group, and 30 healthy people undergoing physical examination in the same period comprised the control group. The expression of miR-614 in tissues and miR-614 in HGC-27 cell line was detected by qRT-PCR, miR-614-mimics was transfected into HGC-27, while miR-614-mimics group, blank control group and negative control group were established respectively.

LncRNA ZEB1-AS1 regulates hepatocellular carcinoma progression by targeting miR-23c.

Background: It has been reported that long-chain non-coding RNA (lncRNA) zinc finger E-box binding homeobox 1 antisense 1 (ZEB1-AS1) is an oncogene in various cancers, including hepatocellular carcinoma (HCC). We investigated the role and mechanism of ZEB1-AS1 as a competitive endogenous RNA (ceRNA) combined with miR-23c in HCC cell proliferation and invasion.

Methods: QRT-PCR was used to detect ZEB1-AS1 and miR-23c expressions in HCC tissues and cells.

Long non-coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA-424-5p/FGF2 .

The aim of the present study was to clarify the effect of long non-coding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) on the proliferation, migration and invasion of osteosarcoma (OS) cells and to explore the potential underlying mechanisms. The expression levels of SNHG1, microRNA (miR)-424-5p and fibroblast growth factor 2 (FGF2) in OS tissues and cells were detected using reverse transcription-quantitative polymerase chain reaction. OS cell proliferation, migration and invasion were analysed by MTT, wound healing and Transwell invasion assays, respectively.

BASP1 Suppresses Cell Growth and Metastasis through Inhibiting Wnt/-Catenin Pathway in Gastric Cancer.

Objective: Our research is designed to explore the function of brain acid soluble protein 1 (BASP1) in the progression of gastric cancer (GC) and its underlying molecular mechanisms.

Methods: In this study, the expression of BASP1 was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in both GC tissue and GC cells. The cell cloning, proliferation, apoptosis, migration, and invasion potential of AGS and HGC-27 cells were, respectively, determined using colony formation assay, 5-ethynyl-20-deoxyuridine (EDU) assay, flow cytometry, and Transwell assay.

The Extent of Therapeutic Central Compartment Neck Dissection in Unilateral cT1N1a or cT2N1a Papillary Thyroid Carcinoma.

Purpose: Papillary thyroid carcinomas (PTCs) frequently metastasize to the central neck compartment. Therapeutic central compartment neck dissection (CCND) is a well-established treatment for PTC nodal metastases; however, the extent to which therapeutic CCND should be performed remains controversial. In this study, we investigated the predictive risk factors for contralateral paratracheal lymph node metastasis (LNM) in unilateral cT1N1a or cT2N1a PTC.

Comparison of clinical diagnostic value of spiral CT with different dose in patients with early-stage peripheral lung cancer.

Purpose: To compare the clinical diagnostic value of spiral CT scan with different dose in patients with early-stage peripheral lung cancer.

Methods: A total of 163 cases of patients with early-stage peripheral lung cancer who came to People's Hospital of Rizhao for treatment from June 2014 to January 2017 were retrospectively analyzed. A total of 78 cases of patients who received low-dose CT scanning were the low-dose group, another 84 cases of patients who received routine dose CT scanning were the routine dose group.

Diffusional kurtosis imaging in evaluation of microstructural changes of spinal cord in cervical spondylotic myelopathy feasibility study.

To explore the value of diffusion kurtosis imaging in the changes of spinal cord microstructures in patients with early cervical spondylotic myelopathy.Twenty nine patients with cervical myelopathy were selected in this study. All images were acquired on a 3.

SNHG16 promotes cell proliferation and migration through sponging miR-132 in melanoma.

Melanoma, which originates from the transformation of normal melanocytes, is one of the three main types of skin cancer. We aimed to explore the functions of SNHG16 and miR-132 in melanoma. CCK-8, Transwell assays were used to measure the viability and migration, respectively.

miR-29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castration-induced osteoporosis through the PI3K/AKT and TGF-β/Smad signaling pathways.

The aim of the present study was to investigate the role of microRNA (miR)-29b in the proliferation and migration of bone marrow mesenchymal stem cells (BMSCs) in rats with castration-induced osteoporosis and the relevant mechanisms. The gene expression profiling microarray technique was utilized to sequence the BMSCs with overexpressed miR-29b. The intersection of the potential targets and the genes downregulated in the sequencing were utilized for GO enrichment analysis.

miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway.

Increasing evidence has indicated that the dysregulation of microRNA (miRNA) occur in the pathogenesis of retinoblastoma (RB). Aim of the present study was to investigate the possible role of miR-494 (miR-494-3p) in RB. It was demonstrated that miR-494 expression was increased in RB tissue samples and cell lines.

Upregulation of miR-1825 inhibits the progression of glioblastoma by suppressing CDK14 though Wnt/β-catenin signaling pathway.

Background: Mounting evidences displayed that miRNAs play crucial roles in tumor initiation and development. However, the regulation and relevant mechanism of miR-1825 in glioblastoma (GBM) remain unclear.

Methods: qRT-PCR was used to detect miR-1825 and CDK14 mRNA expression.