Evaluation of tooth-specific optical properties for the development of a non-invasive pulp diagnostic system using Transmitted-light plethysmography: An in vitro study.

Objectives: Transmitted-light plethysmography (TLP) is an objective and non-invasive pulp diagnosis method that has already been validated for applications for incisors. However, there is a demand for TLP use in the molars, it has not yet been established for this application. This study investigated the optimal light source wavelengths for TLP in premolars, to establish a pulp diagnosis system based on measuring pulpal blood flow.

A Case of Hypophosphatasia Started Enzyme Replacement Therapy Since Babyhood Stage.

Background: Hypophosphatasia (HPP) is an inherited disease caused by low activity of tissue-nonspecific alkaline phosphatase. Dental characteristics include premature loss of primary teeth, enlarged pulp chambers, and enamel hypoplasia. Although enzyme replacement therapy with asfotase alfa was approved in 2015, there are few reports about the dental outcomes of this treatment.

Synthesis and characterization of thermoplastic resin from sugar beet polysaccharides via one-step transesterification.

Lignocellulosic biomass-based plastics provide a sustainable alternative to petroleum-based plastics by converting agricultural by-products into value-added materials, promoting a circular economy. This study investigates the development of thermoplastics from sugar beet pulp (SBP), a by-product rich in cellulose and pectin. A one-pot direct transesterification process was used to fully substitute hydroxy groups in SBP with acyl chains of varying lengths (C2-C10), achieving up to 96 % substitution.

Effect of sulfinate salt on bonding and polymerization of adhesive to intracoronally bleached dentin.

To evaluate the effect of sulfinate salt on the bond performance of a two-step self-etch adhesive to an intracoronally bleached pulpal dentin surface. Intracoronally bleached bovine teeth were treated with or without sulfinate salt (sulfinate agent (SA): Clearfil DC activator) before 2-SEA (Clearfil SE Bond 2) application, while unbleached teeth served as the control (n = 5 teeth). Microtensile bond strength (µTBS) using the bonded surface area of 1 mm at the crosshead speed of 1 mm/min measurements after 24 h storage and thermocycles (TC), degree of conversion (DC) analyses by Raman spectroscopy (n = 3 teeth), ultrastructure of resin-dentin interface (n = 3 teeth), and intracoronally bleached pulp chamber dentin surface (n = 3 teeth) observations by scanning electron microscopy (SEM) were subsequently performed.

Enhancement of Differentiation and Mineralization of Human Dental Pulp Stem Cells via TGF-β Signaling in Low-Level Laser Therapy Using Er:YAG Lasers.

Objectives: Low-level laser therapy (LLLT) using an erbium-doped yttrium aluminum garnet (Er:YAG) laser provides a non-invasive approach applicable to various dental treatments. Here, we investigated the effects of Er:YAG laser irradiation on human dental pulp stem cells (hDPSCs) in an in vitro experiment.

Methods: The hDPSCs were categorized into four groups: laser-irradiated with activators (VLT: activated vitamin D, bone morphogenetic protein receptor inhibitor, and transforming growth factor-beta (TGF-β)) (LLLT(+)VLT), laser-irradiated without activators (LLLT(+)-only), non-irradiated with activators (LLLT(-)VLT), and non-irradiated without activators (control).

Ectopic Salivary Duct Cyst in the Median Mandible: A Case Report with Radiological, Immunohistochemical and Genetic Studies.

Median mandibular cyst is defined as an odontogenic cyst in a rare midline location. In spite of this definition, there have been two reports of a peculiar lesion, so-called "ciliated" median mandibular cyst associated with vital teeth, the origin of which cannot be explained in terms of odontogenic epithelium multipotentiality. We describe a thorough profile of an additional example.

Nanostructural Analysis of Age-Related Changes Affecting Human Dentin.

Human dentin performs its function throughout life, even though it is not remodeled like bone. Therefore, dentin must have extreme durability against daily repetitive loading. Elucidating its durability requires a comprehensive understanding of its shape, structure, and anisotropy at various levels of its structure.

Comparison of the pull-out force of different microtube-based methods in fractured endodontic instrument removal: An in-vitro study.

Background: The fracture of an endodontic instrument within the root canal system can occur during root canal therapy, complicating thorough cleaning and shaping. Consequently, managing the broken fragment becomes crucial.

Methods: Eighty Nickel-titanium (NiTi) #20 K-files (Mani, Tochigi, Japan) were cut 8 mm from the tip, fixed into a corkboard, and classified into five groups (n = 14 each).

Assessment of silver-copper co-loaded mesoporous bioactive glass as an advanced pulp-capping material.

Objectives: To evaluate the multifunctionality of silver-copper co-loaded mesoporous bioactive glass (MBG), with the goal of developing an advanced pulp-capping material.

Methods: The synthesis of materials was conducted using the sol-gel method, following the approach described in previous studies but with some modifications. The composition included 80 mol% SiO₂, 15 mol% CaO, and 5 mol% P₂O₅, with additional components of 5 mol% silver, 5 mol% copper, or 1 mol% silver combined with 4 mol% copper, designated as Ag5/80S, Cu5/80S, or Ag1Cu4/80S, respectively.

Sudachitin Reduces Inflammatory Mediator Expression in Toll-Like Receptor 2 Ligand-Stimulated Human Dental Pulp Cells.

Sudachitin, which is a polymethoxy flavonoid derived from the peer of Citrus sudachi, has several biological properties. However, the effect of sudachitin on human dental pulp cells (HDPCs) remains unclear. The aim of this study was to investigate whether sudachitin could decrease the expression of inflammatory mediators such as cytokines and prostaglandin in HDPCs stimulated with Pam3CSK4, a ligand for toll-like receptor (TLR) 2.

Immune deficiency phenotypes of Il2rg, Rag2 or Il2rg/Rag2 double knockout rats; establishment of human leukemia xenograft models.

Background: Genetically immunodeficient mice lacking Il2rg and Rag2 genes have been widely utilized in the field of biomedical research. However, immunodeficient rats, which offer the advantage of larger size, have not been as extensively used to date. Recently, Severe Combined Immunodeficiency (SCID) rats were generated using CRISPR/Cas9 system, targeting Il2rg and Rag2 in National BioResource Project in Japan.

Dental Pulp Regeneration in Dogs Using a Chemokine Receptor 3 Antagonist Without Transplantation of Dental Pulp Stem Cells.

Introduction: Our previous study showed that transplantation of dental pulp stem cells in combination with a chemokine receptor 3 (CCR3) antagonist into the root canals of aged dogs promoted dental pulp regeneration. In this study, we attempted to regenerate dental pulp in young dogs using a CCR3 antagonist without dental pulp stem cell transplantation.

Methods: The teeth of dogs were histologically evaluated 4 weeks after extraction of the pulp and administration of scaffold materials and CCR3 antagonist (KDH-136) into the root canal.

Saving a hopeless tooth with a four-wall bone defect: A case report.

Background: Recombinant human fibroblast growth factor-2 (rhFGF-2) has been shown to effectively promote the formation of new periodontal tissues, and its efficacy has been demonstrated in clinical settings. Moreover, the clinical and radiographic outcomes in the treatment of periodontal infrabony defects can be improved by using rhFGF-2 in combination with a bone substitute. Here, we present a case of four-wall bone defect in a tooth treated by combination regenerative therapy using rhFGF-2 and beta-tricalcium phosphate (β-TCP).

Controlled Release of Human Dental Pulp Stem Cell-Derived Exosomes from Hydrogels Attenuates Temporomandibular Joint Osteoarthritis.

Temporomandibular joint osteoarthritis (TMJOA) is a painful inflammatory condition that limits mouth opening. Cell-derived exosomes, which have anti-inflammatory effects, are emerging as a treatment for TMJOA. Injection of dental pulp stem cells (DPSCs), which secrete exosomes, can moderate tissue damage in a rat model of TMJOA.

Gene expression profiles in human dental pulp stem cells treated short-term with lipopolysaccharides before and after osteoinduction.

Objectives: Dental pulp stem cells (DPSCs) are essential for reparative dentinogenesis following damage or infection. DPSCs surrounding the blood vessels in the central region of the dental pulp actively proliferate after tooth injury and differentiate into new odontoblast-like cells or odontoblasts to form reparative dentin. However, the signaling pathways involved in undifferentiated and osteodifferentiated DPSCs under inflammatory conditions remain unclear.

Caffeic Acid Phenethyl Ester Enhances Bone Repair-related Factors in MC3T3-E1 Cells.

Apical periodontitis is an inflammatory disease caused by bacterial infection in the root canal that spreads to the apical periodontal tissues, resulting in bone resorption around the root apex as the disease progresses. Vascular endothelial growth factor (VEGF), a growth factor involved in angiogenesis, plays an important role in bone remodeling. We reported that caffeic acid phenethyl ester (CAPE), a bioactive substance of propolis, induces VEGF in odontoblast-like cells and dental pulp cells.

Morphological Changes of the Apical Foramen in Curved Root Canals After Use of Different Heat Treated NiTi Rotary Files: A Scanning Electron Microscopy Study.

Objective: The present study aimed to evaluate the effect of heat-treated nickel titanium (NiTi) files TruNatomy and XP- Endo Shaper on the major apical foramen deformation and enlargement in curved root canals using area, circularity, and Feret's diameter ratio.

Methods: Sixty mesio-buccal roots of maxillary and mandibular molars with Vertucci type II root canal were selected. The teeth were decoronated using a carborundum disc.

Crosstalk between prolactin, insulin-like growth factors, and thyroid hormones in feather growth regulation in neonatal chick wings.

The elongation of primary feathers in neonatal chicks is delayed by the late-feathering K gene located on the Z chromosome. We recently found that the K gene slows feather growth by reducing the number of functional prolactin (PRL) receptor (PRLR) dimers. In this study, we investigated the molecular mechanisms by which PRL promotes feather elongation.

Effect of vasoconstriction by dexmedetomidine in the mandible of rat.

Background: In recent years, dexmedetomidine (DEX) has been proposed as a useful vasoconstrictor for local anesthesia because it is less effective in circulation than clonidine of antihypertensive drugs. In addition, DEX is expected to act as a vasoconstrictor during local anesthesia. However, histomorphometric studies demonstrating that DEX exerts vasoconstrictive effects are lacking.

Scratch-Based Isolation of Primary Cells (SCIP): A Novel Method to Obtain a Large Number of Human Dental Pulp Cells Through One-Step Cultivation.

Dental pulp (DP) is a connective tissue composed of various cell types, including fibroblasts, neurons, adipocytes, endothelial cells, and odontoblasts. It contains a rich supply of pluripotent stem cells, making it an important resource for cell-based regenerative medicine. However, current stem cell collection methods rely heavily on the enzymatic digestion of dissected DP tissue to isolate and propagate primary cells, which often results in low recovery rates and reduced cell survival, particularly from deciduous teeth.

Reconstruction of the finger pulp with palmar digital artery perforator propeller flap using a digital artery perforator at the lateral base of the finger pulp.

Purpose: The digital artery perforator (DAP) flap is used to repair finger pulp defects as it can help preserve the digital artery. The standard DAP flap donor site is dorsal or lateral; however, the color of the flap typically poorly matches that of the finger pulp. The purpose of this study is to report two types of novel palmar DAP flaps.

Inhibitory effect of bovine milk osteopontin on the initial attachment of .

We investigated the effect of bovine milk osteopontin (OPN) on the initial adhesion of to hydroxyapatite. Hydroxyapatite pellets were immersed in a 5.4 µ OPN solution at 37°C for 30 min.

Polycomb protein Bmi1 promotes odontoblast differentiation by accelerating Wnt and BMP signaling pathways.

Bmi1 is a polycomb protein localized in stem cells and maintains their stemness. This protein is also reported to regulate the expression of various differentiation genes. In this study, to analyze the role of Bmi1 during dentinogenesis, we examined the immunohistochemical localization of Bmi1 during rat tooth development as well as after cavity preparation.