Orsay virus infection rate declines with age in .

The intracellular pathogen response is regulated by multiple genes in . How such responses change with age is largely unknown. Thus, we investigated potential age-dependent changes in the immune response to the -specific Orsay virus .

Canine adipose-derived mesenchymal stromal cells inhibit the growth of canine hematologic malignancy cell lines.

Introduction: Intestinal lymphoma may be latent in some dogs with chronic inflammatory enteropathy. Mesenchymal stromal cells (MSCs) have potential therapeutic applications for refractory chronic inflammatory enteropathy, but their impact on the development of potential intestinal lymphomas has not yet been evaluated. Therefore, this study was performed to investigate the effect of canine adipose-derived MSCs (cADSCs) on the growth of canine lymphoma cell lines to assess the safety of MSC-based therapy in terms of pro- and anti-tumorigenic effects.

Development of chimeric antigen receptor T cells targeting cancer-expressing podocalyxin.

Chimeric Antigen Receptor (CAR)-T cell therapy has revolutionized the treatment of CD19-positive B-cell malignancies. However, the field is rapidly evolving to target other antigens, such as podocalyxin (PODXL), a transmembrane protein implicated in tumor progression and poor prognosis in various cancers. This study explores the potential of PODXL-targeted CAR-T cells, utilizing a cancer-specific monoclonal antibody (CasMab) technique to enhance the specificity and safety of CAR-T cell therapy.

Cost-Effectiveness and Clinical Outcomes of Controlled Ovarian Stimulation With Follitropin Delta and Follitropin Alfa: A Retrospective Study.

Aim: This study compared the cost-effectiveness of two recombinant follicle-stimulating hormones (rFSH) formulations, Follitropin Delta and Follitropin Alfa, in controlled ovarian stimulation using cumulative live birth rates as an efficacy indicator.

Methodology: This retrospective study was conducted across five clinics in Japan from April 2022 to December 2023, involving 446 first assisted reproductive technology (ART) cycles (200 with Follitropin Delta and 246 with Follitropin Alfa) were treated with rFSH monotherapy using either Follitropin Delta or Follitropin Alfa. We compared clinical outcomes such as cumulative pregnancy and live birth rates and analyzed cost-effectiveness using the cumulative live birth rates as the efficacy indicator and the incremental cost-effectiveness ratio (ICER).

Locked Lateral Patellar Dislocation Reduced by Arthroscopic Procedure: A Case Report.

Most cases of patellar dislocation can be reduced spontaneously or manually without sedation. To date, only one case of arthroscopic reduction for a lateral locked patellar dislocation has been reported, with a short follow-up period. Herein, we report the case of a 22-year-old man with a lateral locked patellar dislocation for whom we performed arthroscopic reduction and repair of the medial structure, which stabilized the patella medially.

Morbidity Risk of Infectious Gastroenteritis Attributable to Cold and Heat Temperatures: An Analysis of National Surveillance Data in Japan (2000-2019).

Introduction Climate change is a decisive factor affecting human health. While many epidemiological studies have investigated the acute impacts of ambient temperature on mortality and morbidity, the global burden of infectious gastroenteritis linked to temperature changes remains largely unexplored. Therefore, we aimed to examine the exposure-response associations between ambient temperature and infectious gastroenteritis incidence throughout Japan and quantify the temperature-related morbidity burden.

Attitudes and Behaviors Toward Cardiopulmonary Resuscitation Among Healthcare and Non-healthcare Workers in Japan: A Cross-Sectional Study Using a Web-Based Questionnaire.

Background Cardiopulmonary arrest is a leading cause of death and requires swift intervention for survival. Previous studies have highlighted the critical importance of initiating cardiopulmonary resuscitation (CPR) and defibrillation within a limited timeframe. Improving outcomes depends on widespread CPR training, accessible automated external defibrillators (AEDs), and increased public awareness.

Empowering Communication in Medicine: A Workshop Approach to Improve Presentation Skills for Ear, Nose, and Throat Specialists.

Introduction Effective communication is crucial for healthcare professionals, impacting patient care and interdisciplinary collaboration. However, medical education often lacks structured training in communication and presentation techniques. Herein, we evaluate the efficacy of structured workshops aimed at enhancing presentation skills among ear, nose, and throat (ENT) doctors through training in visual material design and concise verbal communication, including elevator pitches.

Tachycardia-Induced Cardiomyopathy Following Prolonged Ritodrine Infusion During Pregnancy: A Case Report.

Preterm birth remains a leading cause of neurodevelopmental disability in offspring, prompting various preventive measures. However, controversies persist surrounding these approaches, particularly regarding beta-mimetic drugs. In Japan, it remains a concerning reality that ritodrine infusion continues to be used for long-term tocolysis in preterm labor, despite the warning issued by the US Food and Drug Administration.

Relationship Between Quadriceps Strength at 6 Months Postoperatively and Improvement in Patient-Reported Knee Function After Anterior Cruciate Ligament Reconstruction.

Background: Understanding the factors associated with poor recovery over time after anterior cruciate ligament reconstruction (ACLR) helps clinicians identify patients who are at risk and targets for an intervention.

Purpose: To determine the factors associated with improvement in subjective knee function from 6 to 12 months after ACLR.

Study Design: Case-control study; Level of evidence, 3.

Association of Preoperative Cartilage T1ρ Values With Progression of Knee Osteoarthritis 10 Years After Anatomical Double-Bundle ACL Reconstruction: Follow-up of a Previous Prospective Study.

Background: The factors contributing to osteoarthritis progression after anterior cruciate ligament (ACL) injury and reconstruction (ACLR) are not fully understood. Quantitative magnetic resonance imaging (MRI) offers a noninvasive way to evaluate cartilage biochemical composition using T1ρ mapping, thereby detecting early cartilage degeneration. The specific impact of preoperative quantitative MRI on long-term outcomes after ACLR remains underreported.

Myeloproliferative neoplasm harboring both monosomy 7 and an fusion gene: Proposal for a new disease entity.

Introduction: We present a case of a 9-year-old girl diagnosed with a myeloproliferative neoplasm (MPN) harboring both monosomy 7 and an ALK/ROS1 fusion gene.

Case Presentation: The neoplasm was resistant to conventional AML chemotherapy and required hematopoietic cell transplantation (HCT) to achieve remission.

Discussion: MPNs with monosomy 7 and ALK/ROS1 fusions occur in a wide age range of children and adults, and require HCT for long-term remission.

HLA-matched related peripheral blood stem cell and bone marrow transplantation with RIC regimens yield comparable outcomes for adult AML.

Introduction: Understanding differences in clinical outcomes between PBSCT and BMT is important, and this study compared outcomes of HLA-matched related PBSCT and BMT using reduced-intensity conditioning (RIC) in adult acute myeloid leukemia (AML) patients.

Methods: Data from 402 patients who underwent either PBSCT ( = 294) or BMT ( = 108) between 2000 and 2022 were analyzed using the Japanese nationwide registry database. The primary endpoint was overall survival (OS), and secondary endpoints included disease-free survival (DFS), non-relapse mortality (NRM), and GVHD.

Association of the nutritional risk index recorded prior to allogeneic hematopoietic cell transplantation with the clinical prognosis in children.

Introduction: The nutritional risk index (NRI), calculated using serum albumin levels and body weight ratio is a known prognostic factor in adult hematopoietic cell transplantation (HCT). However, its usefulness in pediatric HCT settings remains unclear.

Methods: In a retrospective study, we examined pre-transplant NRI impact on outcomes in 82 pediatric patients undergoing allogeneic HCT.

Tumor-Induced Osteomalacia Localized by Systemic Venous Sampling and Ga-DOTATOC Positron Emission Tomography.

Tumor-induced osteomalacia is characterized by hypophosphatemia and fragility fractures caused by fibroblast growth factor 23 (FGF23)-producing tumors. We report a case of tumor-induced osteomalacia in which the tumor location could be determined by gallium 68 (Ga)-DOTATOC positron emission tomography (PET)/computed tomography (CT). A 74-year-old woman had recurrent fractures and bone pain.

A Novel Pathogenic Gene Variant in Hyperparathyroidism-jaw Tumor Syndrome.

Hyperparathyroidism-jaw tumor syndrome (HPT-JT) is a rare hereditary disorder caused by pathogenic gene variants. We report the case of a patient with HPT-JT who carried a novel germline pathogenic variant. A 27-year-old woman presented with thirst, polyuria, fatigue, constipation, and a history of fibro-osseous mandible lesions and endometrial polyps.

Durvalumab-induced Type 1 Diabetes in a Patient With Pre-existing GADA-positive Diabetes and Preserved Insulin Secretion.

Predicting the onset of type 1 diabetes mellitus (T1D) in patients treated with immune checkpoint inhibitors (ICI) remains challenging. ICI-induced T1D (ICI-T1D) is a rare but serious complication that leads to complete insulin depletion. While diabetes-associated autoantibodies, such as glutamic acid decarboxylase antibodies (GADA), are typically absent in non-ICI-related fulminant T1D, they are relatively common in ICI-T1D.

Application of virtual reality technology improves the functionality of brain networks in individuals experiencing pain.

Medical procedures are inherently invasive and carry the risk of inducing pain to the mind and body. Recently, efforts have been made to alleviate the discomfort associated with invasive medical procedures through the use of virtual reality (VR) technology. VR has been demonstrated to be an effective treatment for pain associated with medical procedures, as well as for chronic pain conditions for which no effective treatment has been established.

Blue Electroluminescent Carbon Dots Derived from Victorian Lignite.

Carbon dots (CDs) derived from natural products have attracted considerable interest as eco-friendly materials with a wide range of applications, such as bioimaging, sensors, catalysis, and solar energy harvesting. Among these applications, electroluminescence (EL) is particularly desirable for light-emitting devices in display and lighting technologies. Typically, EL devices incorporating CDs feature a layered structure, where CDs function as the central emissive layer, flanked by charge transport layers and electrodes.

Imine Synthesis by Engineered d-Amino Acid Oxidase from Porcine Kidney.

Various symmetric and asymmetric imines were synthesized using the novel amine oxidase, obtained as variants of d-amino acid oxidase (pkDAO) from porcine kidney (Y228L/R283G) and (I230A/R283G). Active primary imines produced as intermediates in the oxidation of methylbenzylamine (MBA) derivatives were trapped by aliphatic, aromatic amines and diamines as nucleophiles forming new imines. ()-Fluoro-MBA was the best substrate for symmetric imine synthesis, providing almost stoichiometric conversion (100 mM) and achieving nearly 100% yield.

Analysing head and trunk motion in the judo osoto-gari technique: relationship to sweeping-leg velocity.

Background: Osoto-gari is a leg throw technique that primarily relies on the hip extension to initiate the sweeping motion of the leg. A high sweep contact velocity is a crucial factor in efficiently executing this technique. While some literature emphasises whole-body coordination in the leg-sweeping action, the roles of trunk and head motion remain unclear.

A luminous and young galaxy at = 12.33 revealed by a JWST/MIRI detection of Hα and [O iii].

The James Webb Space Telescope has discovered a surprising population of bright galaxies in the very early Universe (≲500 Myr after the Big Bang) that is hard to explain with conventional galaxy-formation models and whose physical properties are not fully understood. Insight into their internal physics is best captured through nebular lines, but at these early epochs, the brightest of these spectral features are redshifted into the mid-infrared and remain elusive. Using the mid-infrared instrument onboard the James Webb Space Telescope, here we present a detection of Hα and doubly ionized oxygen ([O iii] 4959,5007 Å) from the bright, ultra-high-redshift galaxy candidate GHZ2/GLASS-z12.

Effects of physiotherapy on degenerative cerebellar ataxia: a systematic review and meta-analysis.

Background: Evidence of the effectiveness of physiotherapy, including muscle strength training, coordination training, aerobic exercise, cycling regimen, balance training, gait training, and activity of daily living training, in patients with degenerative cerebellar ataxia (DCA) was insufficient for clinical decision making. We aimed to explore clinical outcomes and examine the parameters associated with physical impairment and activity in people with DCA based on preregistration (PROSPERO: CRD42024493883).

Methods: The PubMed, Cochrane Library, CHINAL, and PEDro databases were searched for relevant randomized controlled trials (RCTs).