Transcriptional dynamics during karyogamy in rice zygotes.

Upon fertilization, male and female nuclei fuse to form the zygotic nucleus in angiosperms. Karyogamy is considered to be essential for proper embryogenesis; however, the transcriptional dynamics during karyogamy in plant zygotes remain unclear. In this study, we performed a single-cell transcriptome analysis of rice zygotes at six early developmental stages (15 min, 30 min, 1 h, 2 h, 4 h, and 6 h after gamete fusion) to reveal gene expression profiles during karyogamy in plant zygotes.

Plasmodium falciparum histidine-rich protein 2 exhibits cell penetration and cytotoxicity with autophagy dysfunction.

Plasmodium falciparum is a major cause of severe malaria. This protozoan infects human red blood cells and secretes large quantities of histidine-rich protein 2 (PfHRP2) into the bloodstream, making it a well-known diagnostic marker. Here, however, we identified PfHRP2 as a pathogenic factor produced by P.

The N-Terminal Fragment of Urine Titin Is Not a Product of Degradation by Calpain 3.

Introduction: A 20 kDa fragment at the N-terminus of titin is highly excreted in the urine of patients with Duchenne muscular dystrophy (DMD), making urine titin a prominent biomarker for muscle breakdown. This N-terminal fragment is presumed to be a product of degradation by a protein-degrading enzyme, calpain 3; however, whether calpain 3 is required remains unclear. We aimed to determine whether urine titin elevation occurs in the absence of calpain 3.

Phase transformation and metallurgical characterization of heat-treated nickel-titanium rotary instruments using differential scanning calorimetry, X-ray diffraction, and energy dispersive spectrometry.

Objective: The present study aimed to evaluate the phase transformation behavior and elemental analysis of thermomechanical-treated nickel-titanium (NiTi) rotary instruments, TruNatomy (Dentsply Sirona), HyFlex CM (coltene, Whaledent), and Neoendo Flex (Orikam healthcare India), using differential scanning calorimetry (DSC), X-ray diffraction (XRD), and energy dispersive X-ray spectrometry.

Materials And Methods: A total of 18 NiTi rotary instruments, TruNatomy, Hyflex CM, Neoendo Flex, taper. 04, size 25 (except TruNatomy, size 26) were selected and were divided into three groups ( = 6).

A novel potential measurement indicator with objective and quantitative effect for trigeminal neuralgia: fractional anisotropy in MR-DTI.

Objectives: To investigate the effect of diffusivity metrics of magnetic resonance diffusion tensor imaging (MR-DTI) in the assessment of treatment effects.

Methods: MR-DTI examination for trigeminal neuralgia (TN) patients and the diffusivity metrics of the trigeminal ganglion (TG) were analyzed. Before and after the percutaneous stereotactic radiofrequency rhizotomy (PSR) operation, the treatment effect was assessed using pain scores and MR-DTI.

Canopy elastic turbulence: Insights and analogies to canopy inertial turbulence.

Canopy flows occur when a moving fluid encounters a matrix of free-standing obstacles and are found in diverse systems, from forests and marine ecology to urban landscapes and biology (e.g. cilia arrays).

pulmonary disease with organizing pneumonia: A case report and review of literature.

, a rapidly growing non-tuberculous mycobacterium, rarely causes pulmonary diseases. A patient was admitted to our hospital with a fever and cough. Chest radiography revealed consolidation in the right middle lung.

Neurosurgical strategy based on the type of occult spinal dysraphism in omphalocele-exstrophy-imperforate anus-spinal defects complex: A review of 10 cases.

Background: Omphalocele-exstrophy-imperforate anus-spinal defects (OEIS) complex is a rare, life-threatening congenital malformation primarily treated with abdominogenital repair. The optimal indication and timing of neurosurgical interventions for the associated spinal cord lesions remains insufficiently studied. We reviewed spinal dysraphism in OEIS to evaluate the best timing for neurosurgical intervention.

Utility of intraoperative fluorescence imaging with indocyanine green for diagnosing spinal schwannoma: A case report.

Background: The delayed-window indocyanine green (DWICG) technique is useful for the removal of brain and spinal tumors.

Case Description: A 41-year-old female presented with lower left back and radicular pain. An magnetic resonance imaging (MRI) revealed an extramedullary L3 lesion located ventrally in the spinal canal that appeared to be a schwannoma.

An experimental chimeric hepatitis E virus vaccine elicits both local and systemic immune responses.

Introduction: The development of a hepatitis E virus (HEV) vaccine is critical, with ORF2 capsid protein as the main target. We previously demonstrated that oral coadministration of recombinant ORF2 with immunomodulatory bacterium-like-particles (IBLP) induces a specific immune response in mice, particularly using IBLP derived from IBL027 (IBLP027), which was effective in eliciting a local humoral response. IBLP are non-live bacteria with adjuvant and carrier properties, serving as a platform for exposing proteins or antigens fused to LysM (lysine motif) domains, protein modules that bind to cell wall polysaccharides like peptidoglycan.

Idiopathic infantile hypercalcemia with a variant triggered by vitamin D supplementation in fortified milk: A case report.

Idiopathic infantile hypercalcemia (IIH) is characterized by hypercalcemia, nephrocalcinosis, vomiting, dehydration, and failure to thrive. It is caused by the presence of biallelic loss-of-function variants in the locus. Although hypercalcemia has been linked to the consumption of vitamin D-fortified milk, no reports have documented its role in triggering IIH in patients with variants.

Current understanding and perspectives on growth and long-acting GH therapy in Japan.

From the perspective of clinical pediatric endocrinology, progress in molecular biology over the past few decades has improved our understanding of growth physiology. This progress was particularly conspicuous in the early era of Sanger-based sequencing and has continued into the current era of next-generation sequencing. The first half of this review summarizes the current understanding about growth.

Complex equilibrium involving aldosterone underlies the pathophysiology of renovascular hypertension.

The mechanisms underlying the maintenance of hypertension in renovascular hypertension (RVH) are not well understood. To test the current concept of RVH pathophysiology, circulating aldosterone levels in clinical cases were investigated through a literature survey of pediatric cases. Fifty-four patients with documented aldosterone levels were identified.

Newborn screening for congenital adrenal hyperplasia: Utility of liquid chromatography with tandem mass spectrometry as a secondary test.

In Japan, newborn screening (NBS) for congenital adrenal hyperplasia (CAH) began in 1989. NBS is useful for early diagnosis and preventing gender misidentification, however, it has a higher false positive rate for CAH compared to other diseases detected by neonatal screening. Recently, it has become clear that using liquid chromatography with tandem mass spectrometry (LC-MS/MS) for second-tier testing reduces false positive rates and repeat blood sampling.

Factors predictive of serum cortisol in pediatric patients with acute physiological stress: a cohort study.

Measuring cortisol is crucial for assessing adrenal function in patients under stress; however, its value can fluctuate owing to various clinical factors. This study aimed to identify predictors of cortisol levels in pediatric patients with acute physiological stress. Children who were urgently admitted to the general ward or pediatric intensive care unit for acute illness or postoperative care were enrolled, while those with suspected adrenal function abnormalities or on current steroid therapy were excluded.

Identification of a novel missense variant in the gene in a Japanese pedigree with familial neurohypophyseal diabetes insipidus.

Familial neurohypophyseal diabetes insipidus is a rare genetic disease caused by gene variants and is characterized by progressive polyuria and polydipsia in early childhood. Herein, we have reported the clinical symptoms and genetic test results of a Japanese patient with a family history of polyuria and polydipsia for over five generations. The proband was a 6-yr-old boy who was referred for the evaluation of polyuria and polydipsia.

Wieacker-Wolff syndrome with hyperinsulinemic hypoglycemia successfully treated using diazoxide: A case report.

Wieacker-Wolff syndrome (WRWF) is an X-linked genetic disorder characterized by neuromusculoskeletal abnormalities caused by loss-of-function variants of the gene. Here, we report the case of a male infant with WRWF manifesting as multiple joint contractures and congenital anomalies at birth. He underwent gastrostomy to treat the gastroesophageal reflux disease, which caused mixed apnea and transient bradycardia.

haploinsufficiency in a mother-daughter pair with young-onset diabetes with and without neonatal hypoglycemia.

The ATP-binding cassette transporter subfamily C member 8 (ABCC8) regulates insulin secretion from β-cells. Loss- and gain-of-function variants of have been implicated in neonatal hyperinsulinemic hypoglycemia and young-onset diabetes, respectively. Although some patients with variants have been reported to exhibit both neonatal hypoglycemia and young-onset diabetes, the molecular and clinical characteristics of this atypical phenotype remain unknown.

Solitary median maxillary central incisor syndrome caused by 22q11.2 microdeletion.

Solitary median maxillary central incisor (SMMCI) syndrome, the mildest form of the holoprosencephaly spectrum, is a rare anomaly characterized by the presence of a single midline central incisor in both the deciduous and permanent dentitions. Affected individuals can present with additional midline defects beyond dental findings. The 22q11.