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Evaluation of susceptibility testing methods for polymyxin.
Int J Infect Dis
July 2010
Department of Laboratory Medicine, Jai Prakash Narain Apex (JPNA) Trauma Centre, All India Institute of Medical Sciences (AIIMS), New Delhi, 110029 India.
Background: The widespread resistance in Gram-negative bacteria has necessitated evaluation of the use of older antimicrobials such as polymyxins. In the present study we evaluated the different susceptibility testing methods for polymyxins B and E against Gram-negative bacteria using the new Clinical and Laboratory Standards Institute (CLSI) guidelines.
Methods: The susceptibility of 281 multidrug-resistant (MDR) Gram-negative bacteria (GNB) to polymyxin B was evaluated, comparing broth microdilution (BMD; reference method), agar dilution, E-test, and disk diffusion.