Phylogenomic analysis of metagenome-assembled genomes indicates new taxa in the order Spirochaetales and proposal of Thalassospirochaeta sargassi gen. nov. sp. nov. from seaweeds.

Six metagenome-assembled genomes (JB008, JB007, JB015, JB003, JB004, and JB002) belonging to the order Spirochaetales were generated from seaweed samples collected from the Gulf of Mannar, India. The binned genomes JB008 and JB007 shared highest 16S rRNA gene identity of 94.9 % and 92.

, a Novel Planctomycete Isolated from an Algal Mat, Displays Antimicrobial Activity.

Axenic cultures of two strains, JC673 and JC717, both belonging to the phylum , were isolated from distinct geographical locations in India. Strain JC673 was obtained from algal mats of a wetland situated in the state of Kerala, India, while strain JC717 originated from the Central Marine Fisheries Research Institute (CMFRI), state of Tamil Nadu, India. The two strains share 99.

Sinomonas cellulolyticus sp. nov., isolated from Loktak lake.

A novel cellulolytic strain JC656 was isolated from the rhizosphere soil of Alisma plantago-aquatica of floating island (Phumdis) of Loktak lake, Manipur, India. The 16S rRNA gene sequence similarities between strain JC656 and other Sinomonas type strains ranged between 98.5 and 97.

Development and Validation of a Method for Trace Level Zinc Quantification in Pharmaceutical Zinc Supplements Using a Carboxyl Functional Group Packed Column and Refractive Index Detector.

Background: Zinc helps with cell division, growth, wound healing, and carbohydrate breakdown. Humanbeings have to obtain zinc from food or supplements because our bodies do not produce it naturally. In view of the greater advantages (such as low cost, time of analysis, and stability-indicating) compared to other quantification methods (titration, ion chromatography, Atomic absorption spectroscopy) proposed in the literature, a refractive index detector coupled with HPLC has been used in quantification of zinc.

Blastopirellula sediminis sp. nov. a new member of Pirellulaceae isolated from the Andaman and Nicobar Islands.

Two cream-coloured strains (JC732, JC733) of Gram-stain negative, mesophilic, catalase and oxidase positive, aerobic bacteria which divide by budding, form crateriform structures, and cell aggregates were isolated from marine habitats of Andaman and Nicobar Islands, India. Both strains had genome size of 7.1 Mb and G + C content of 58.

Stable isotope-assisted metabolite profiling reveals new insights into L-tryptophan chemotrophic metabolism of Rubrivivax benzoatilyticus.

Anoxygenic photosynthetic bacteria (APB) are metabolically versatile, capable of surviving with an extended range of carbon and nitrogen sources. This group of phototrophic bacteria have remarkable metabolic plasticity in utilizing an array of organic compounds as carbon source/electron donors and nitrogen sources with sophisticated growth modes. Rubrivivax benzoatilyticus JA2 is one such photosynthetic bacterium utilizes L-tryptophan as nitrogen source under phototrophic growth mode and produces an array of indolic compounds of biotechnological significance.

Phylogenomic analysis of the genus : proposal for division of this genus into the emended genus and two novel genera gen. nov. and gen. nov.

The members of the genus are key players in the removal of petroleum hydrocarbons from polluted marine environments. More than half of the species were described in the last decade using 16S rRNA gene phylogeny and genomic-based metrics. However, the 16S rRNA gene identity (<94 %) between some members of the genus suggested their imprecise taxonomic status.

Revisiting the taxonomy of the genus Rhodopirellula with the proposal for reclassification of the genus to Rhodopirellula sensu stricto, Aporhodopirellula gen. nov., Allorhodopirellula gen. nov. and Neorhodopirellula gen. nov.

The current genus Rhodopirellula consists of marine bacteria which belong to the family Pirellulaceae of the phylum Planctomycetota. Members of the genus Rhodopirellula are aerobic, mesophiles and chemoheterotrophs. The here conducted analysis built on 16S rRNA gene sequence and multi-locus sequence analysis based phylogenomic trees suggested that the genus is subdivided into four clades.

Heterostructures of polyaniline and Ce-ZnO nanomaterial coated flexible PET thin films for LPG gas sensing at standard environment.

The n-type Ce doped ZnO (Ce-ZnO) and p-type polyaniline (PANI) heterojunction were successfully synthesized via simple chemical solution method for sensing liquefied petroleum gas (LPG) at standard environment. The morphology and structures of as-prepared Ce-ZnO & PANI nanoparticles were analyzed by numerous kinds of techniques. Ce-ZnO & PANI nanoparticles were mixed with n-methylpyrrolidone (NMP) which is coated over the gold coated PET electrode by doctor blade method and dried overnight at 60 °C to form p-n junction.

Phylogenomic analysis of a metagenome-assembled genome indicates a new taxon of an anoxygenic phototroph bacterium in the family Chromatiaceae and the proposal of "Candidatus Thioaporhodococcus" gen. nov.

In this study, three metagenome-assembled genomes of a sediment sample were constructed. A Bin1 (JB001) genome was identified as a photo-litho-auto/heterotroph (purple sulfur bacteria) bacterium with the ability to fix nitrogen, tolerate salt, and to produce bacteriochlorophyll a. It has a genome length of 4.

Draft Genome Sequence of sp. Strain JC586, Isolated from Soil Sediment of the Floating Islands of Loktak Lake, India.

The 3.52-Mbp whole-genome sequence of a sp. strain isolated from soil sediment of the floating islands of Loktak Lake is reported.

Paludisphaera rhizosphaereae sp. nov., a new member of the family Isosphaeraceae, isolated from the rhizosphere soil of Erianthus ravennae.

Two axenic cultures of Planctomycetota were isolated from distinct geographical locations of India. Strain JC665 was isolated from a rhizosphere soil of Loktak lake, Manipur, whereas strain JC747 was isolated from a soil sediment at Pallikkara village, Kerala, India. The two closely related strains shared the highest 16S rRNA gene sequence identity (94.

Neoroseomonas marina sp. nov., Isolated from a Beach Sand.

A pink-pigmented bacterium (strain JC162 = KCTC 32190) was isolated from a beach sand sample. Cells were Gram-stain-negative, coccoid, non-motile, and strictly aerobic. EzBioCloud BLAST search of 16S rRNA gene sequence showed that strain KCTC 32190 had the highest sequence identity to the members of the genus Neoroseomonas and was closely related to N.

A genomic overview including polyphasic taxonomy of Thalassoroseus pseudoceratinae gen. nov., sp. nov. isolated from a marine sponge, Pseudoceratina sp.

A pink-coloured, salt- and alkali-tolerant planctomycetal strain (JC658) with oval to pear-shaped, motile, aerobic, Gram-negative stained cells was isolated from a marine sponge, Pseudoceratina sp. Strain JC658 shares the highest 16S rRNA gene sequence identity with Maioricimonas rarisocia Mal4 (< 89.2%) in the family Planctomycetaceae.

Influence of multi valent states of vanadium ions in ZnO doped novel calcium fluoro phosphate bio glasses.

ZnO-CaF-PO glasses doped with different concentrations of VO (ranging from 0 to 1.0 mol %) were prepared. The prepared bio glasses are soaked in SBF for duration of 2, 3, 7 and 10 days in separate plastic containers and then kept in incubator maintained at body temperature 36.

Dynamics of proteo-metabolome from Rubrivivax benzoatilyticus JA2 reveals a programmed switch-off of phototrophic growth, leading to a non-cultivable state as a hyperglycemic effect.

Anoxygenic phototrophic bacteria display phenomenal metabolic plasticity leading to distinct phenotypes. Extracellular elevated glucose levels limit photosynthesis in photosynthetic organisms; diversely, cause oxidative stress with ROS generation and "diabetic" like situation in non-photosynthetic organisms. In this study, longer incubations of externally provided glucose (22 mM) inhibited photosynthetic machinery in a phototrophic bacterium, Rubrivivax benzoatilyticus.

Total synthesis of (3R, 4S)-4-hydroxylasiodiplodin.

An efficient stereoselective total synthesis of (3R, 4S)-4-hydroxylasiodiplodin has been accomplished starting from known starting materials. The key steps involved in this synthesis are Stille cross coupling, alkylation of 1,3-dithiane and Yamaguchi macrolactonization.

Phylotaxogenomics for the Reappraisal of the Genus With the Creation of Six New Genera.

The genus is a significant group of bacteria which is invariably of great clinical and ecological importance. Previous studies have shown that the genus is polyphyletic in nature. Our present study focused on generating a lucid understanding of the phylogenetic framework for the re-evaluation and reclassification of the genus Phylogenetic studies based on the 16S rRNA gene and 92 concatenated genes suggested that the genus is heterogeneous, forming seven major groups.

Phylo-taxogenomics of the genus Tautonia with descriptions of Tautonia marina sp. nov., Tautonia rosea sp. nov., and emended description of the genus.

Four strains of Planctomycetes, (JC636, JC649, JC650, JC657) which are all salt and alkali tolerant, pink coloured, with spherical to oval shaped, Gram-stain-negative, non-motile cells were isolated from different regions of Chilika lagoon, India. All strains have obligate requirement for N-acetylglucosamine (NAG) and share highest 16S rRNA gene sequence identity with members of the genus Tautonia (<95%) of the family Isosphaeraceae. The 16S rRNA gene sequence identity between strains was >99.

ZrCaP thermoluminescent bio glass, structure and elasticity.

Phosphate glasses of calcium oxide have been well proved materials for various bio bones and dental implants. However, still there is a lot of scope and demand to produce efficient elastic bio implants and resource. In view of this, ZrCaP phosphate materials are prepared by using melt quenching method.

Crateriforma spongiae sp. nov., isolated from a marine sponge and emended description of the genus "Crateriforma".

A Gram-stain-negative, aerobic, pear to oval shaped, rosette forming bacterium with crateriform structures well distributed on the cell surface designated as strain JC647 was isolated from a sponge specimen belonging to the genus Spongia. Strain JC647 reproduces through budding. Strain JC647 shared highest 16S rRNA gene sequence identity of 99.

"Sporotan" a new fluorescent stain for identifying cryptic spores of Rhodobacter johrii.

We propose a new fluorescent stain "sporotan" and staining protocol which aid in the identification of cryptic endospores which are otherwise mistaken as poly-β-hydroxyalkanoate granules.

Acteoside Isolated from Colebrookea oppositifolia Smith Attenuates Epilepsy in Mice Via Modulation of Gamma-Aminobutyric Acid Pathways.

The present study was aimed to evaluate the anticonvulsant activity of acteoside and explore its mechanism of action. Initially, the acteoside was evaluated in maximal electroshock (MES) and pentylenetetrazole (PTZ)-induced convulsions, and later it was evaluated against N-methyl-D-aspartic acid (NMDA)-induced mortality in Swiss albino mice. Based on the response in these models, further evaluations were performed to explore the mechanism of action.

Tryptophan, a non-canonical melanin precursor: New L-tryptophan based melanin production by Rubrivivax benzoatilyticus JA2.

Melanins are chemically diverse ubiquitous pigments found across the life forms synthesized via different biochemical pathways mainly from L-tyrosine or acetyl CoA. Though few reports suggest the possibility of tryptophan-based melanin synthesis, however, such tryptophan-based melanin and its biosynthesis remained a biochemical riddle. Here we report tryptophan-based melanin production by bacterium, Rubrivivax benzoatilyticus JA2.