5 results match your criteria: "JC WILT Infectious Disease Research Center[Affiliation]"

Clinical samples are routinely inactivated before molecular assays to prevent pathogen transmission. Antibody-based assays are sensitive to changes in analyte conformation, but the impact of inactivation on the analyte detectability has been overlooked. This study assessed the effects of commonly used inactivation-methods, Triton X-100 (0.

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Background: Adolescent girls and young women aged 15‒24 years in sub-Saharan Africa are at disproportionate risk of human immunodeficiency virus (HIV) infection. Given the known association between vaginal microbial dysbiosis and HIV susceptibility, we performed an age-stratified analysis of the vaginal microbiome in South African women and compared this to their risk of HIV acquisition.

Methods: Vaginal microbiome data were generated by mass spectrometry-based proteomic analysis of cervicovaginal lavages collected from participants (n = 688) in the Centre for the AIDS Programme of Research in South Africa (CAPRISA) 004 trial.

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Severe viral respiratory infections in children with loss-of-function mutations.

Proc Natl Acad Sci U S A

August 2017

Global Health Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne, Lausanne, 1015, Switzerland;

Viral respiratory infections are usually mild and self-limiting; still they exceptionally result in life-threatening infections in previously healthy children. To investigate a potential genetic cause, we recruited 120 previously healthy children requiring support in intensive care because of a severe illness caused by a respiratory virus. Using exome and transcriptome sequencing, we identified and characterized three rare loss-of-function variants in which encodes an RIG-I-like receptor involved in the sensing of viral RNA.

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Microbial translocation and microbiome dysbiosis in HIV-associated immune activation.

Curr Opin HIV AIDS

March 2016

aDepartment of Pharmaceutics, University of Washington bWashington National Primate Research Center, Seattle, Washington, USA cCentre for the AIDS Programme of Research (CAPRISA), Durban, South Africa dDepartment of Medical Microbiology and Infectious Diseases, University of Manitoba eNational Laboratory for HIV Immunology, JC Wilt Infectious Disease Research Center, Public Health Agency of Canada, Winnipeg, Manitoba, Canada fDepartment of Medicine Solna, Center for Molecular Medicine, Karolinska Institute, Karolinska, Sweden.

Purpose Of Review: This article describes the mechanisms and consequences of both microbial translocation and microbial dysbiosis in HIV infection.

Recent Findings: Microbes in HIV are likely playing a large role in contributing to HIV pathogenesis, morbidities and mortality. Two major disruptions to microbial systems in HIV infection include microbial translocation and microbiome dysbiosis.

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