In silico analysis of sporulene biosynthesis pathway genes in the members of the class Bacilli.

Sporulene, a pentacyclic triterpenoid, was discovered in Bacillus subtilis and is associated with bacterial endospores. However, the study was not further extended, leaving a trail of questions. One such question is what diversity of sporulenes exists among spore-forming members? Considering the sporulene biosynthesis pathway as a fundamental tool to survey the distribution of this molecule, a genome mining study was conducted.

Development and validation of a stability-indicating, single HPLC method for sacubitril-valsartan and their stereoisomers and identification of forced degradation products using LC-MS/MS.

The aim of this research work was to develop and validate a stability-indicating, single reversed-phase HPLC method for the separation of five impurities, including enantiomers, diastereomers, and degradation products in sacubitril-valsartan tablets. The method was developed using a Chiralcel OJ-RH column (150 × 4.6 mm, 5 μm) at 45°C with a gradient program of (T/%B) 0.

Stability-indicating HPLC method development and validation of rivaroxaban impurities and identification of forced degradation products using LC-MS/MS.

The current aim of this study is to develop a simple, sensitive, and robust analytical method that can separate the Rivaroxaban and its related impurities by using HPLC. This new method can separate enantiomers and all the process-related impurities of rivaroxaban. This method can also be used to determine the assay of rivaroxaban in drug substances and drug products.

A simple isocratic LC method for quantification of trace-level inorganic degradation impurities (ferricyanide, ferrocyanide, nitrite, and nitrate) in sodium nitroprusside injection and robustness by quality using design approach.

This study developed and validated a trace-level quantification inorganic impurities method using reversed-phase HPLC and performed the robustness check using quality-by-design approach by varying the multiple factors simultaneously. This method is economical and simple and exhibits its stability-indicating nature [for the determination of ferrocyanide ([Fe(CN)₆] ), ferricyanide ([Fe(CN) ] ), nitrate (NO ), and nitrite (NO )] in sodium nitroprusside (SNP) drug substance and liquid dosage form. Chromatographic separation was achieved using a USP L43 column (ACE PFP, 150 × 4.

Aquisphaera insulae sp. nov., a new member in the family Isosphaeraceae, isolated from the floating island of Loktak lake and emended description of the genus Aquisphaera.

Strain JC669 was isolated from a floating island of Loktak lake, Manipur, India and shares the highest 16S rRNA gene sequence identity with Aquisphaera giovannonii OJF2. The novel strain is aerobic, Gram negative, light pink-coloured, non-motile, NaCl intolerant and spherical to oval-shaped. It grows in the form of single cells or aggregates and possibly forms structures which appear like fruiting bodies.

Uncovering the hidden bacterial ghost communities of yeast and experimental evidences demonstrates yeast as thriving hub for bacteria.

Our major concern was to address "yeast endobacteria" which was based on a few reports in the recent past where bacteria may find yeast as a niche for survival. In this study, we report the microbiota of twenty-nine axenic yeast cultures recovered from different habitats based on their 16S rRNA gene-amplicon metagenomes. Yeasts were identified based on D1/D2 or ITS gene sequences.

"Candidatus Laterigemmans baculatus" gen. nov. sp. nov., the first representative of rod shaped planctomycetes with lateral budding in the family Pirellulaceae.

Two axenic cultures of Planctomycetes were isolated from distinct geographical regions of the east coast of India. The two closely related strains (JC640 and CH01) showed <93.3% 16S rRNA gene sequence identity with members of the genus Roseimaritima followed by Rhodopirellula (<91%).

Isoptericola sediminis sp. nov., Isolated from Chilika Lagoon.

A yellow-colored, Gram-stain-positive, rod shaped, non-motile bacterium, designated as strain JC619, was isolated from the sediment of Chilika lagoon, India. Strain JC619 shows highest 16S rRNA gene sequence similarity (99.08%) with Isoptericola chiayiensis KCTC 19740 followed by Isoptericola halotolerans KCTC 19046 (98.

Mesobacillus aurantius sp. nov., isolated from an orange-colored pond near a solar saltern.

An endospore producing, strict aerobic, Gram-stain-positive, orange-colored colony forming bacterium designated as strain JC1013 was isolated from an orange pond near a solar saltern of Tamil Nadu, India. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain was affiliated to the family Bacillaceae of the phylum Firmicutes. Strain showed highest 16S rRNA gene sequence identity of 98.

Descriptions of Roseiconus nitratireducens gen. nov. sp. nov. and Roseiconus lacunae sp. nov.

Two pink-coloured, oxidase-catalase-positive, salt and alkali-tolerant planctomycetal strains (JC635 and JC645) with pear to spherical-shaped, Gram-stain-negative, motile cells were isolated from Chilika lagoon, India. Both strains share highest 16S rRNA gene sequence identity with members of the genus Rhodopirellula (< 94%) and Roseimaritima (< 94%) of the family Pirellulaceae. The 16S rRNA sequence identity between the strains JC635 and JC645 is 96.

Arenibacter lacus sp. nov., Isolated from Chilika Lagoon, India.

A shiny dark yellow pigmented, Gram-stain-negative, rod-shaped, non-motile bacterium, designated as strain JC631, was isolated from the sediment ecosystem of Chilika lagoon, India. Strain JC631 tolerates up to pH 10 and NaCl up to 3% (w/v). MK6 is the only respiratory quinone.

Paludisphaera soli sp. nov., a new member of the family Isosphaeraceae isolated from high altitude soil in the Western Himalaya.

A novel strain of Planctomycetes, designated JC670, was isolated from a high altitude (~ 2900 m above sea level) soil sample collected from Garhwal region in the Western Himalaya. Colonies of this strain were observed to be light pink coloured with spherical to oval shaped cells having crateriform structures distributed all over the cell surface. The cells divide by budding.

Streptomyces marianii sp. nov., a novel marine actinomycete from southern coast of India.

A novel marine actinomycete strain designated ICN19 was isolated from the subtidal sediment of Chinnamuttam coast of Kanyakumari, India and subjected to polyphasic taxonomic analysis. Neighbour-joining tree based on 16S rRNA gene sequences of validly described type strains had revealed the strain ICN19 formed distinct cluster with Streptomyces wuyuanensis CGMCC 4.7042, Streptomyces tirandamycinicus HNM0039 and Streptomyces spongiicola HNM0071.

sp. nov., a new member of isolated from Chilika lagoon.

Strain JC651 was isolated from a sediment sample collected from Chilika lagoon, which is one of the world's most important brackish water lakes with estuarine characteristics. Colonies of this strain are light pink and cells are Gram-stain negative, spherical to pear shaped and form rosettes. Strain JC651 grows well up to pH 9.

sp. nov., an indole-producing bacterium isolated from the rhizosphere of an orchid, .

A Gram-stain-negative, non-motile, coccoid-shaped, catalase- and oxidase-positive, non-denitrifying, neutrophilic bacterium designated as strain JC501 was isolated from an epiphytic rhizosphere of an orchid, , growing in the Western Ghats of India. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain JC501 belonged to the genus and had the highest levels of sequence identity with KKL-A5 (98.9 %), WPAn02 (97.

sp. nov., isolated from a fresh water pond.

A phototrophic bacterium, designated as strain JA983, was isolated from a freshwater pond in Gujarat, India. The strain was yellowish brown, catalase- and oxidase-positive, rod-to-oval shaped, Gram-stain-negative and motile. Growth was observed at 20-35 °C.

sp. nov., a phototrophic bacterium.

An oval- to rod-shaped, motile, Gram-stain-negative, oxidase-positive, catalase-negative, pink-coloured phototrophic bacterium (designated as strain JA968) was isolated from an estuary near Pata, Gujarat, India. Cells had an intracytoplasmic membrane architecture as lamellae and divided by budding. Strain JA968 had bacteriochlorophyll- and spirilloxanthin series carotenoids as photosynthetic pigments.

Biological hydrogen production: molecular and electrolytic perspectives.

Exploration of renewable energy sources is an imperative task in order to replace fossil fuels and to diminish atmospheric pollution. Hydrogen is considered one of the most promising fuels for the future and implores further investigation to find eco-friendly ways toward viable production. Expansive processes like electrolysis and fossil fuels are currently being used to produce hydrogen.

Rhodobacter alkalitolerans sp. nov., isolated from an alkaline brown pond.

An alkali-tolerant, Gram-stain-negative, motile, rod-to-oval-shaped, yellowish brown-colored, phototrophic bacterium, designated as strain JA916, was isolated from an alkaline brown pond in Gujarat, India. The DNA G + C content of the strain JA916 was 65.1 mol%.

Rhodococcus electrodiphilus sp. nov., a marine electro active actinobacterium isolated from coral reef.

An electrogenic bacterium was isolated from a marine coral, designated as strain JC435 and its taxonomic status examined by using a polyphasic approach. Results from the 16S rRNA gene sequence study showed that the isolate belonged to the genus Rhodococcus and formed a cluster with Rhodococcus ruber KCTC 9806 (99.5 % 16S rRNA gene sequence similarity) and Rhodococcus aetherivorans JCM 14343 (99.

Planctopirus hydrillae sp. nov., an antibiotic producing Planctomycete isolated from the aquatic plant Hydrilla and its whole genome shotgun sequence analysis.

An antibiotic producing novel Planctomycete strain, designated JC280, was isolated from the surface of the plant Hydrilla verticillata collected from an alkaline lake (Buffalo lake), University of Hyderabad, Hyderabad, India. The morphological and chemotaxonomic properties of strain JC280 were in agreement with the characteristics of the genus Planctopirus. The cell shape was spherical to ovoid and some were tear drop shaped.

Chryseobacterium salipaludis sp. nov., isolated at a wild ass sanctuary.

A Gram-stain-negative, rod-shaped, non-motile, aerobic bacterium was isolated from a sediment sample obtained from a wild ass sanctuary in Gujarat, India. The strain designated JC490 was oxidase- and catalase-positive. The 16S rRNA gene sequence analysis and sequence comparison data indicated that strain JC490 was a member of the genus Chryseobacterium and was closely related to Chryseobacterium jeonii AT1047 (96.