CAD-CAM complete digital dentures: An improved clinical and laboratory workflow.

The purpose of this article was to present a novel clinical workflow for the fabrication of complete dentures using computer-aided design and computer-aided manufacturing (CAD-CAM) technology. The dental technique consists of 3 clinical steps and 2 laboratory phases that result in the production of 2 CAD-CAM milled complete denture bases with prefabricated teeth. The integration of analog and digital procedures and materials maximizes their benefits in the planning and fabrication of complete dentures, with the goal of improving clinical outcomes.

An antibody-free bio-layer interferometry biosensor for immunoglobulin G1 detection in human serum by using molecularly imprinted polynorepinephrine.

Bio-Layer Interferometry (BLI) has emerged as a versatile technique in affinity-based biosensing, analogous to Surface Plasmon Resonance. BLI enables real-time, label-free detection, and quantification of biomolecular interactions between an immobilized receptor and an analyte in solution. The BLI sensor comprises an optical fiber with an internal reference layer at the end and an external biocompatible layer where biological receptors are immobilized and exposed to the solution.

Ascertainment of small airway dysfunction using oscillometry to better define asthma control and future risk: are we ready to implement it in clinical practice?

The small airways comprise generations 8 to 23 of the bronchial tree, consist of airways with an internal diameter <2mm, and are classically difficult to assess and treat in persistent asthma. Small airways dysfunction (SAD) is integral to the asthma management paradigm as it is associated with poorer symptom control, greater levels of type 2 inflammation, and has been proposed as a potential treatable asthma trait. Although identification of SAD by oscillometry has been found to be clinically useful in managing asthma, very few physicians, including specialists, use this technique as part of standard or adjunct evaluation of lung function to diagnose asthma, grade severity of airway obstruction, ascertain disease control or the risk for future exacerbations or to make management decisions.

A topographic approach to the markers of macrophage/ microglia and other cell types in high grade glioma.

In glioblastoma, glioma-associated microglia/macrophages (GAMs) represent the major population of tumor infiltrating cells, with up to one half of the cells of the tumor mass. Recent studies have shown that microglia are involved in the maintenance of immunological homeostasis and protection against autoimmunity. However, despite the growing body of evidence on the topic, many aspects are yet to be clarified.

AI-driven identification of a novel malate structure from recycled lithium-ion batteries.

The integration of Artificial Intelligence (AI) into the discovery of new materials offers significant potential for advancing sustainable technologies. This paper presents a novel approach leveraging AI-driven methodologies to identify a new malate structure derived from the treatment of spent lithium-ion batteries. By analysing bibliographic data and incorporating domain-specific knowledge, AI facilitated the identification and structure refinement of a new malate complex containing different metals (Ni, Mn, Co, and Cu).

Comparison of HDR-brachytherapy and tomotherapy for the treatment of non-melanoma skin cancers of the head and neck.

Purpose: This study aims to investigate and compare High Dose Rate Brachytherapy (HDR-BT) with Helical Tomotherapy (HT) treatment plans. The focus is on small target volumes near radiation-sensitive organs in the ocular region, to evaluate the advantages of these techniques in treating skin cancer.

Methods: This retrospective observational analysis included patients who underwent skin cancer HDR-BT Freiburg flap treatment between 2019 and 2023.

Haploinsufficiency of PDE2A causes in mice increased exploratory behavior associated with upregulation of neural nitric oxide synthase in the striatum.

Phosphodiesterase 2 A (PDE2A) function is stimulated by cGMP to catabolize cAMP. However, neurological and neurochemical effects of PDE2A deficiency are poorly understood. To address this gap, we studied behavioral characteristics and cerebral morpho-chemical changes of adult male heterozygous C57BL/6-PDE2A+/- (HET), and wild type C57BL/6-PDE2A+/+ (WT) mice.

Shielding siRNA by peptide-based nanofibers: An efficient approach for turning off EGFR gene in breast cancer.

Peptide-based self-assembled nanosystems show great promise as non-viral gene and siRNA delivery vectors. In the current study, we designed and functionalized nanofibers for the delivery of siRNA, targeting and silencing EGFR gene overexpressed in triple-negative breast cancer. The nanofiber-mediated siRNA delivery was characterized in terms of zeta potential, morphology, and structural stability by circular dichroism spectroscopy.

Conservative Treatment Of Uterine Myomas: A Network Meta-analysis Of Randomized Controlled Studies.: NMA For Uterine Myomas Conservative Treatment.

Objective: To comparatively evaluate the effectiveness of uterine artery embolization (UAE), focused ultrasound (HIFU), radiofrequency ablation (RFT), and laparoscopic/laparotomic surgery in the conservative treatment of uterine fibroids DATA SOURCES: The research was performed via electronic databases PubMed, EMBASE, and Cochrane Library, using the PRISMA standards.

Methods Of Study Selection: The network included 10 randomized trials between 2000 and 2024 and 1002 randomized subjects.

Tabulation: The Network meta-analysis (NMA) was carried out with subroutine netmeta on R.

The AβA2V Paradigm: From Molecular Insights to Therapeutic Strategies in Alzheimer's disease and primary tauopathies.

Alzheimer's disease, the leading cause of dementia globally, represents an unresolved clinical challenge due to its complex pathogenesis and the absence of effective treatments. Considering the multifactorial etiology of the disease, mainly characterized by the accumulation of amyloid β plaques and neurofibrillary tangles of tau protein, we discuss the A673V mutation in the gene coding for the amyloid precursor protein, which is associated with the familial form of Alzheimer's disease in a homozygous state. The mutation offers new insights into the molecular mechanisms of the disease, particularly regarding the contrasting roles of the A2V and A2T mutations in amyloid β peptide aggregation and toxicity.

Next-generation sequencing and drug resistance mutations of HIV-1 subtypes in people living with HIV in Sicily, Italy, 2021-2023.

Objectives: HIV-1 infection continues to be a significant public health concern, notwithstanding the expanded utilization of antiretroviral treatment (ART), due to the emergence of drug resistance. The prevalence of transmitted drug resistance remains uncertain, particularly concerning integrase inhibitors. This study aimed to assess the extent of HIV resistance in both ART-naïve and experienced individuals living with HIV (PLHIV) at the University Hospital in Palermo, Italy.

Infectivity in full-term placenta of Zika viruses with different lipid profiles.

Among flaviviruses, Zika virus (ZIKV) is the only arbovirus officially recognized as a teratogenic agent, as a consequence of its ability to infect and cross the placental barrier causing congenital malformation in the fetus. While many studies have focused on understanding ZIKV pathogenesis during pregnancy, the viral mechanisms affecting fetal development remain largely unclear. In this study, we investigated ZIKV virulence in placental trophoblasts, using viruses with distinct lipid profiles.

Step-by-step hysteroscopic treatment of FIGO type 3 myoma with the cold loop technique.

Objective: To demonstrate the "cold loop technique" for the hysteroscopic treatment of FIGO type 3 myomas.

Design: Step-by-step demonstration of the technique using educative video.

Subject: A 45-year-old infertile patient with repeated oocyte donor IVF failures affected by a FIGO type 3 myoma.

Multi-omic markers of Intraductal Papillary Mucinous Neoplasms progression into pancreatic cancer.

Pancreatic ductal adenocarcinoma (PDAC) is the most lethal and common form of pancreatic cancer, it has no specific symptoms, and most of the patients are diagnosed when the disease is already at an advanced stage. Chemotherapy typically has only a modest effect, making surgery the most effective treatment option. However, only a small percentage of patients are amenable to surgery.

Complete versus culprit-lesion-only percutaneous coronary intervention after out-of-hospital cardiac arrest in patients with multivessel disease.

Background: Multivessel coronary artery disease (MVD) represents a common finding at invasive coronary angiography (ICA) among out-of-hospital cardiac arrest (OHCA) survivors. However, optimal invasive treatment strategy for MVD in OHCA remains unknown. Our study aims to assess if complete revascularization improves one-year clinical outcomes in these patients.

Are you tuned in to others' mind? A cross-modal evaluation of affective theory of mind in people with Parkinson's disease.

Affective Theory of Mind (ToM) is the ability to understand other peoples' emotional states and feelings. Several studies showed impaired affective ToM abilities in people with Parkinson's disease (PD). However, most studies tested this ability by using single-stimulus modality tasks (visual cues).

Longitudinal pathways from emotional abuse to problematic gaming in adolescents: The role of psychoticism.

Background: Problematic gaming (PG) has emerged as a major concern due to its potential impact on adolescents' everyday lives. Emotional abuse (EA) and psychoticism are considered factors influencing PG, but their long-term interactions have not been extensively studied. This research investigates these relationships over time using the Interaction of Person-Affect-Cognition-Execution (I-PACE) model.

Decoding neuronal networks: A Reservoir Computing approach for predicting connectivity and functionality.

In this study, we address the challenge of analyzing electrophysiological measurements in neuronal networks. Our computational model, based on the Reservoir Computing Network (RCN) architecture, deciphers spatio-temporal data obtained from electrophysiological measurements of neuronal cultures. By reconstructing the network structure on a macroscopic scale, we reveal the connectivity between neuronal units.

Experience based efficient approach for DNA-led identification of highly carbonized human remains.

In case of severely burned bodies, victim identification by visual or fingerprints recognition is often prevented by altered body conditions. To overcome these circumstances, different techniques are available. Among these, the most reliable is molecular identification, especially in cases of detached body parts.

Do machine learning methods solve the main pitfall of linear regression in dental age estimation?

Introduction: Age estimation is crucial in forensic and anthropological fields. Teeth, are valued for their resilience to environmental factors and their preservation over time, making them essential for age estimation when other skeletal remains deteriorate. Recently, Machine Learning algorithms have been used in age estimation, demonstrating high levels of accuracy.

Cationic liposomes as carriers of natural compounds from plant extract.

Lipid-based nanocarriers provide versatile platforms for the encapsulation and delivery of many different bioactive compounds to improve the solubility, stability and therapeutic efficacy of bioactive phyto-compounds. In this study, liposomes were used to load leaf extract of Coffea Arabica, which is known to be rich beneficial substances such as alkaloids, flavonoids, etc. The aim of this work is to optimize the valorization of agricultural wastes containing natural antioxidants.

Improving the physical health of overweight/obese people suffering from severe mental disorders: What is the role of antipsychotic drugs and of lifestyle psychosocial interventions?

People with severe mental disorders experience premature mortality compared with the general population. Several factors contribute to the mortality gap, including the adoption of unhealthy lifestyle behaviours, poor screening for physical illnesses, difficulties in accessing healthcare facilities, specific clinical features of mental disorders and some pharmacological treatment such as antipsychotic medications with serious metabolic side effects. In the present study, carried out in the framework of the LIFESTYLE trial, a funded nationwide multicentric study, we aimed to assess the impact of different antipsychotics in mediating the effectiveness of psychosocial intervention on healthy lifestyle behaviours.