Solid-state fermentation with RT-3 enhanced the nutritional properties of soybeans.

Fermented soybean products are favorite foods worldwide because of their nutritional value and health effects. In this study, solid-state fermentation (SSF) of soybeans with RT-3 was performed to investigate its nutraceutical potential. A rich enzyme system was released during SSF.

Mechanism of As(III) removal properties of biochar-supported molybdenum-disulfide/iron-oxide system.

Sulfate (SO) and hydroxyl-based (HO) radical are considered potential agents for As(III) removal from aquatic environments. We have reported the synergistic role of SO and HO radicals for As(III) removal via facile synthesis of biochar-supported SO species. MoS-modified biochar (MoS/BC), iron oxide-biochar (FeO@BC), and MoS-modified iron oxide-biochar (MoS/FeO@BC) were prepared and systematically characterized to understand the underlying mechanism for arsenic removal.

Controlling ischemic cardiovascular disease: from basic mechanisms to clinical management.

Progress in cardiovascular disease understanding and management continues at an exponential pace. Our understanding of the molecular basis of disease is enhanced by newer molecular measurement techniques, sophisticated models of physiological protein functions, understanding of the genetic foundation for diseases, and the incorporation of population genetic tools in our clinical analysis. In this review, I discuss prevention and therapy of coronary stenosis impeding coronary flows, prevention of acute and chronic manifestation of coronary flow impairment, and interfering with myocardial manifestation of acute or chronic deprivation of coronary flow.

The role of Ca2+ in coupling cardiac metabolism with regulation of contraction: in silico modeling.

The heart adapts the rate of mitochondrial ATP production to energy demand without noticeable changes in the concentration of ATP, ADP and Pi, even for large transitions between different workloads. We suggest that the changes in demand modulate the cytosolic Ca2+ concentration that changes mitochondrial Ca2+ to regulate ATP production. Thus, the rate of ATP production by the mitochondria is coupled to the rate of ATP consumption by the sarcomere cross-bridges (XBs).

Preface: cardiac control pathways: signaling and transport phenomena.

Signaling is part of a complex system of communication that governs basic cellular functions and coordinates cellular activity. Transfer of ions and signaling molecules and their interactions with appropriate receptors, transmembrane transport, and the consequent intracellular interactions and functional cellular response represent a complex system of interwoven phenomena of transport, signaling, conformational changes, chemical activation, and/or genetic expression. The well-being of the cell thus depends on a harmonic orchestration of all these events and the existence of control mechanisms that assure the normal behavior of the various parameters involved and their orderly expression.

Excessive prolongation of prothrombin time among patients treated with warfarin and admitted to the emergency room.

Introduction: Patients treated with warfarin are uncommonly admitted with excessive prolongation of international normalized ratio (INR) of prothrombin time (PT) to the emergency room (ER). Here we wanted to evaluate the reasons behind this prolongation, clinical and laboratory parameters, course and outcome of these patients.

Patients And Methods: Consecutive patients admitted to the ER with prolonged PT-INR>6 under warfarin treatment and who gave sufficient information regarding history of warfarin treatment, were followed prospectively.

Subtractive hybridization techniques to study cellular senescence.

Molecular and cellular processes related to the senescence syndrome are determined by programs of differential gene expression. Subtractive cDNA hybridization is a powerful approach to identify and isolate differentially expressed genes in various systems. A highly effective method, termed suppression subtractive hybridization (SSH), has been applied for the generation of subtracted cDNA library of senescing leaves.

Multiplexing for optimal lighting.

Imaging of objects under variable lighting directions is an important and frequent practice in computer vision, machine vision, and image-based rendering. Methods for such imaging have traditionally used only a single light source per acquired image. They may result in images that are too dark and noisy, e.

Contrast enhanced multi-detector computed tomography coronary angiography versus conventional invasive quantitative coronary angiography in acute coronary syndrome patients-correlation and bias.

Background: Previous studies that compared multi-detector computed tomography (MDCT) non-invasive coronary angiography with conventional coronary angiography, did not assessed the ability of MDCT to detect stenotic lesions correctly in acute coronary syndromes (ACS) patients. The aim of the present study was to assess prospectively the correlation and bias between 16-slice MDCT coronary angiography and quantitative coronary angiography analysis (QCA) in these patients.

Methods: Patients underwent electrocardiogram-gated, 16-slice MDCT coronary angiography and routine invasive percutaneous coronary angiography with quantitative coronary angiography (QCA) analysis blinded to MDCT results.

Involvement of Rho kinase pathway in the mechanism of renal vasoconstriction and cardiac hypertrophy in rats with experimental heart failure.

Rho-dependent kinases serve as downstream effectors of several vasoconstrictor systems, the activities of which are upregulated in congestive heart failure (CHF). We evaluated renal and cardiac effects of Y-27632, a highly selective Rho kinase inhibitor, in an experimental model of volume-overload CHF. Effects of acute administration of Y-27632 (0.

Hormonal suppressive therapy for endometriosis may not improve patient health.

Objective: To critically examine the possible association between hormonal treatment of endometriosis and ovarian cancer.

Result(s): The malignant potential of endometriosis has been suggested by several clinical studies. Although controversial, ovarian carcinoma of the endometrioid and clear cell subtypes has been associated with endometriosis, particularly among subjects with a longstanding disease.

CT diagnosis of traumatic bronchial rupture in children.

Bronchial rupture is a rare and serious complication of blunt chest trauma in children. The diagnosis of this injury is challenging and requires a high degree of clinical suspicion. It is frequently associated with other severe injuries that may draw the focus of attention away from this potentially catastrophic but treatable injury.

Glutamic acid 160 is the acid-base catalyst of beta-xylosidase from Bacillus stearothermophilus T-6: a family 39 glycoside hydrolase.

A beta-xylosidase from Bacillus stearothermophilus T-6 was cloned, overexpressed in Escherichia coli and purified to homogeneity. Based on sequence alignment, the enzyme belongs to family 39 glycoside hydrolases, which itself forms part of the wider GH-A clan. The conserved Glu160 was proposed as the acid-base catalyst.

Simulation of a cold-stressed finger including the effects of wind, gloves, and cold-induced vasodilatation.

The thermal response of fingers exposed to cold weather conditions has been simulated. Energy balance equations were formulated, in a former study, for the tissue layers and the arterial, venous, and capillary blood vessels. The equations were solved by a finite difference scheme using the Thomas algorithm and the method of alternating directions.

The role of growth hormone in ovulation induction.

During the last few years the potential importance of intraovarian regulators of follicular development has been recognized, and the effects of various growth factors on granulosa cell responses to gonadotrophins have been extensively investigated. Ovarian responses to exogenous gonadotrophins were improved by adjuvant growth hormone (GH) treatment of GH-deficient patients, and responses to hMG were apparently predicted by estimated GH reserve. However, the results of different studies of GH/hMG cotreatment were equivocal.

Delayed fluorescence of porphyrins in different media.

Lifetimes of TPPS4 (meso-tetraphenylporphine tetrasulfonate) triplet states were measured for liquid solutions of different acidity and viscosity and as a function of acceptor concentration for different acceptors (bovine serum albumin, tryptophan and furfuryl alcohol). Triplet lifetimes were estimated by monitoring the decay of TPPS4 delayed fluorescence of E-type. The lifetime of delayed fluorencence depends on the concentration of O2, since the latter is an effective quencher of the triplet state.

Differential reversal of lipophilic antifolate resistance in mammalian cells with modulators of the multidrug resistance phenotype.

Chinese hamster ovary (CHO) T19 cells express a stable P-glycoprotein (P-170)-dependent multidrug resistance (MDR) phenotype and display a 24- to 29-fold cross-resistance to the lipophilic antifolates piritrexim (PTX) and trimetrexate (TMTX). We have examined the ability of various modulators of the MDR phenotype to sensitize T19 cells to TMTX and PTX in a clonogenic assay. An almost complete reversal of TMTX resistance in T19 cells was achieved with several modulators of the MDR phenotype whereas only a partial sensitization of T19 cells to PTX was obtained with the most potent modulator.

A hypernychthemeral sleep-wake syndrome: a treatment attempt.

The wake and sleep-onset times of a patient with a sleep-wake cycle longer than 24 hr were recorded by the patient for 4 years. During this time, the patient found himself unable to maintain a 24-hr sleep-wake schedule. When treated with 1-2 mg clonazepam, taken nightly, he was able to become entrained to a 24-hr day.