Cobalt oxide nanoparticles mediate tau denaturation and cytotoxicity against PC-12 cell line.

It has not been well explored how NPs may induce some adverse effects on the biological systems. In this research, the interaction of cobalt oxide NPs (CoO NPs) with tau protein and PC-12 cell line, as nervous system models, was investigated with several approaches including fluorescence spectroscopy, CD spectroscopy, docking study, MTT, LDH, AO/EB dual staining, and flow cytometry assays. Fluorescence investigation displayed that CoO NPs spontaneously mediate the formation of a static complex with tau protein through hydrogen bonds and van der Waals forces.

Probing the interaction of zero valent iron nanoparticles with blood system by biophysical, docking, cellular, and molecular studies.

Human exposure to nanoparticles (NPs) is inevitable as NPs become more widely applied and, as a result, nanotoxicology study is now gaining attention. Herein, the interaction of zero valent iron NPs (ZVFe-NPs) with human hemoglobin (Hb) was evaluated using a variety of techniques including fluorescence spectroscopy, far circular dichroism (CD) spectroscopy as well as docking study. Also, the cytotoxicity of ZVFe-NPs on the human lymphocyte cell line as a model of blood system cell line was investigated by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), acridine orange/ethidium bromide (AO/EB) staining, flow cytometry, and real-time PCR assays.

Human hemoglobin adsorption onto colloidal cerium oxide nanoparticles: a new model based on zeta potential and spectroscopy measurements.

The nanoparticle (NP)-induced conformational changes of protein and NP agglomeration have gained a remarkable interest in medical and biotechnological fields. Herein, the effect of human hemoglobin (Hb) on the colloidal stability of cerium oxide NP (CNP) was investigated by dynamic light scattering (DLS), zeta potential, and TEM analysis. In addition, the effect of CNP on the heme degradation and structural changes of Hb was studied using fluorescence, circular dichroism (CD), and UV-visible (UV-vis) spectroscopic methods.