The apoptotic impact of nisin as a potent bacteriocin on the colon cancer cells.

Nisin is a polycyclic peptide containing 34 amino acids produced by Lactococcus lactis during fermentation. Recently, researchers considered nisin as an anticancer peptide. Herein, the authors aim to evaluate the nisin effects on the apoptosis stimulation in the colon cancer cell line.

A novel recombinant vaccine candidate comprising PBP2a and autolysin against Methicillin Resistant Staphylococcus aureus confers protection in the experimental mice.

The methicillin-resistant Staphylococcus aureus infection is a hot topic area in microbiology research. Here a novel vaccine candidate consisting of recombinant PBP2a and autolysin proteins were used. The proteins over expressed in E.

Interaction of manganese nanoparticle with cytochrome c: A multi-spectroscopic study.

In this paper, the conformational changes of cytochrome c (cyt c) upon interaction with manganese nanoparticle (Mn-NP) were examined using dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), zeta potential, fluorescence spectroscopy, and circular dichroism (CD) spectroscopy methods. DLS and TEM analysis exhibited the structure of Mn-NP was less than 50nm. FTIR bands were similar to those reported for Mn-NP.

Systems Biology Approach to Bioremediation of Nitroaromatics: Constraint-Based Analysis of 2,4,6-Trinitrotoluene Biotransformation by Escherichia coli.

Microbial remediation of nitroaromatic compounds (NACs) is a promising environmentally friendly and cost-effective approach to the removal of these life-threating agents. () has shown remarkable capability for the biotransformation of 2,4,6-trinitro-toluene (TNT). Efforts to develop as an efficient TNT degrading biocatalyst will benefit from holistic flux-level description of interactions between multiple TNT transforming pathways operating in the strain.

Photo-catalytic, anti-bacterial, and anti-cancer properties of phyto-mediated synthesis of silver nanoparticles from Artemisia tournefortiana Rchb extract.

Metal nanoparticles have largely been investigated due to their potential medicinal activities. This study demonstrates the biological properties of green-synthesized silver nanoparticles (AgNPs) by using Artemisia tournefortiana Rchb ethanol extract. Instrumentations such as ultraviolet-visible spectra analysis, high-resolution transmission electron microscopy, scanning electron microscopy, energy-dispersive X-ray analysis, X-ray diffraction, and Fourier transform infrared spectroscopy were used to reveal the synthesized AgNPs.

Synthesis and characterization of cobalt-supported catalysts on modified magnetic nanoparticle: Green and highly efficient heterogeneous nanocatalyst for selective oxidation of ethylbenzene, cyclohexene and oximes with molecular oxygen.

In this study, a new supported cobalt nanocatalyst has been described. The FeO magnetic nanoparticles (FeO MNPs) modified by SiO/aminopropyl trimethoxy silane/cyanuric chloride (FeO@SiO-APTMS/CC) utilized for anchoring metformin-cobalt complex (FeO Ms@SiO-APTMS/CC/Met@Co(II)). The structure of novel complex well defined by elemental analysis, ICP, AAS, BET, FT-IR, EDX, SEM, TEM, DLS, XRD, TG-DTG, VSM and XPS.

Efficacy of nanocurcumin supplementation on insulin resistance, lipids, inflammatory factors and nesfatin among obese patients with non-alcoholic fatty liver disease (NAFLD): a trial protocol.

Objectives: Different studies have been conducted on the role of curcumin in health since having multiple properties, including antioxidant and anti-inflammatory effects. Due to the lack of studies regarding curcumin effects on obese patients with non-alcoholic fatty liver disease (NAFLD), our protocol was designed to assess nanocurcumin impacts on blood sugar, lipids, inflammatory indices, insulin resistance and liver function, especially by nesfatin.

Setting: This trial will be conducted in the Oil Company central hospital of Tehran, Iran with a primary level of care.

Molecular origin of drug release by water boiling inside carbon nanotubes from reactive molecular dynamics simulation and DFT perspectives.

Owing to their nanosized hollow cylindrical structure, CNTs hold the promise to be utilized as desired materials for encapsulating molecules which demonstrate wide inferences in drug delivery. Here we evaluate the possibility of drug release from the CNTs with various types and edge chemistry by reactive MD simulation to explain the scientifically reliable relations for proposed process. It was shown that heating of CNTs (up to 750 K) cannot be used for release of incorporated drug (phenylalanine) into water and even carbonated water solvent with very low boiling temperature.

Exploring the Origin of the Generalized Anomeric Effects in the Acyclic Nonplanar Systems.

Contrary to the published conclusions in the literature concerning the origin of the generalized anomeric relationships in open-chain nonplanar systems, its origin has remained an open question. In order to explore the origin of the generalized anomeric relationships in open-chain nonplanar systems, we assessed the roles and contributions of the effective factors on the conformational properties of methyl propargyl ether (1), methyl propargyl sulfide (2), and methyl propargyl selenide (3) by means of the G3MP2, CCSD(T), MP2, LC-ωPBE, and B3LYP methods and natural bond orbital (NBO) interpretations. We examined the contributions of the hyperconjugative interactions on the conformational preferences of compounds 1-3 by the deletions of the orbitals overlapping from the Fock matrices of the gauche- and anti-conformations.

Anticonvulsant Effects of Aerial Parts of Verbena officinalis Extract in Mice: Involvement of Benzodiazepine and Opioid Receptors.

To evaluate the anticonvulsant activity of the aerial parts of Verbena officinalis used traditionally by local Iranians for the treatment of convulsion. The anticonvulsant activity of the extract was assessed in pentylenetetrazole (PTZ) and maximal electroshock (MES) induced seizures in mice. Diazepam was used as reference drug.

Antioxidant, antibacterial and anticancer properties of phytosynthesised Podlech extract mediated AgNPs.

The focus of this study is on a rapid and cost-effective approach for the synthesis of silver nanoparticles (AgNPs) using Podlech aerial parts extract and assessment of their antioxidant, antibacterial and anticancer activities. The prepared AgNPs were determined by ultraviolet-visible spectroscopy, X-ray diffraction, Fourier transform infra-red spectroscopy, transmission electron microscopy, scanning electron microscopy, energy-dispersive spectroscopy, and dynamic light scattering and zeta-potential analysis. The AgNPs and extract were evaluated for their antiradical scavenging activity by 2, 2-diphenyl, 1-picryl hydrazyl assay and anticancer activity against colon cancer (human colorectal adenocarcinoma cell line 29) compared with normal human embryonic kidney (HEK293) cells.

The role of nitric oxide in anticonvulsant effect of nanocurcumine on pentylenetetrazole-induced seizure in mice.

Unlabelled: A plant alkaloid obtained from Curcuma longa, curcumin possesses anti-oxidant and anti-inflammatory effects. Nanoformulations have been developed for preclinical studies which demonstrate enhanced therapeutic efficacy. Effect of acute intraperitoneal (i.

Recombinant PBP2a as a vaccine candidate against methicillin-resistant Staphylococcus aureus: Immunogenicity and protectivity.

Methicillin-resistant Staphylococcus aureus infections are focal and development of an effective vaccine can help to control this infection. Here, recombinant PBP2a was studied in mouse model. Following the preparation of recombinant PBP2a, Balb/c mice were injected subcutaneously with 20 μg of r-PBP2a formulated in Freund's adjuvant three times with three weeks intervals with proper control group.

Hydrogen purification performance of a nanoporous hexagonal boron nitride membrane: molecular dynamics and first-principle simulations.

Membranes have attracted much attention for the efficient separation of gas mixtures, due to their specific structural and unique properties. In this work, density functional theory (DFT) and molecular dynamic (MD) simulations have been employed to evaluate the performance of nanoporous hexagonal boron nitride (h-BN) monolayers for hydrogen purification. Various porous membranes were designed, and full structural relaxation was carried out by using DFT calculations and then MD simulations to investigate the H purification performance of the nanoporous h-BN membranes.

Determination of Bortezomib in API Samples Using HPLC: Assessment of Enantiomeric and Diastereomeric Impurities.

A new, normal phase high performance liquid chromatography (NP-HPLC) method was developed for separation of Bortezomib (BZB) enantiomers and quantitative determination of (1S,2R)-enantiomer of BZB in active pharmaceutical ingredient (API) samples. The developed method was validated based on International Conference on Harmonisation (ICH) guidelines and it was proved to be accurate, precise and robust. The obtained resolution (RS) between the enantiomers was more than 2.

Fluorescent multi-responsive cross-linked P(N-isopropylacrylamide)-based nanocomposites for cisplatin delivery.

Magnetic, pH and temperature-sensitive, poly(N-isopropylacrylamide) (PNIPAM)-based nanocomposites with fluorescent properties were synthesized by free radical copolymerization-cross linking of NIPAM, N,N-dimethylaminoethyl methacrylate (DMAEMA) and 4-acrylamidofluorescein (AFA). The model anti-cancer drug, cisplatin (CDDP), was loaded into the resulted nanogel. For the production of CDDP-loaded nanocomposite, FeO magnetic nanoparticles (MNPs) and CDDP were loaded into the nanogel.

Chemical composition and biological properties of Maroofi.

is an indigenous plant which is frequently used as a spice in Avraman-Kurdistan region of Iran. The present study aimed to investigate the chemical composition, antimicrobial and antioxidant properties of the . In addition, rosmarinic acid and total phenolic content of was assessed by spectrophotometric method and HPTLC.

A spectroscopic study on the absorption of carbonic anhydrase onto the nanoporous silica nanoparticle.

Herein, KIT-6 nanoporous silica nanoparticles were used as a solid support for immobilization of bovine carbonic anhydrase, isoform II (BCA II). The zeta potential study revealed that KIT-6 and BCA II provided negative (-13.58±1.

Tunable phenol remediation from wastewater using SWCNT-based, sub-nanometer porous membranes: reactive molecular dynamics simulations and DFT calculations.

The importance and impact of the application of CNT membranes with sub-nanometer pores for effective water purification are marvelous. Here we demonstrate, by reactive MD simulations, that CNT membranes can efficiently reject phenol due to molecular size exclusion effects and yield high permeability of water. The water flux in armchair CNTs with a pore diameter of about 7 Å is 1.

Interaction of iron nanoparticles with nervous system: an in vitro study.

Nanoparticles (NPs) are one of the interesting and widely studying issues mainly because of their particular physico-chemical features and broad applications in the field of biomedical sciences, such as diagnosis and drug delivery. In this study, the interaction of iron nanoparticles (Fe-NPs) with Tau protein and PC12 cell, as potential nervous system models, was investigated with a range of techniques including dynamic light scattering, intrinsic fluorescence spectroscopy, circular dichroism, [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium-bromid] assay, and acridine orange/ethidium bromide (AO/EB) dual staining method. An inverse correlation between Stern and Volmer constant (K) and temperature indicated a probable static quenching mechanism occurred between Tau protein and Fe-NPs.

Flexible magnetic polyurethane/FeO nanoparticles as organic-inorganic nanocomposites for biomedical applications: Properties and cell behavior.

Nowadays, the discovery of cell behaviors and their responses in communication with the stem cell niches and/or microenvironments are one of the major topics in tissue engineering and regenerative medicine. In this study, incorporated organic-inorganic polyurethane (PU) nanocomposites were prepared for better understanding of cell signaling and the effect of magnetite nanoparticles on cell proliferation and cell responses. The properties of PU-IONs were evaluated by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), atomic-force microscopy (AFM), differential scanning calorimetry (DSC), X-ray diffraction (XRD) and electrochemical impedance spectroscopy (EIS).

Preparation of core/shell nanostructure Fe(3)O(4)@PEG400-SO(3)H as heterogeneous and magnetically recyclable nanocatalyst for one-pot synthesis of substituted pyrroles by Paal-Knorr reaction at room temperature.

An efficient procedure has been proposed for the loading of sulfonic acid groups on the surface of polyethylene glycol 400 (PEG400)-encapsulated FeO nanoparticles to synthesize a core-shell FeO@PEG400-SOH nano catalyst. Surface functionalization of magnetic particles in such a way is a refined method of bridging the gap amongst heterogeneous and homogeneous catalysis. The procured nano catalyst was classified through Fourier transformed infrared spectroscopy (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), wavelength-dispersive X-ray spectroscopy (WDX), thermogravimetric analysis (TGA), vibrating sample magnetometer (VSM), X-ray photoelectron spectroscopy (XPS), BET, and back titration.

Effect of CYP2D6 polymorphisms on the pharmacokinetics of propafenone and its two main metabolites.

Aim Of The Study: Propafenone (PPF) is an antiarrhythmic drug, metabolized mainly by CYP2D6 to 5-hydroxypropafenone (5OH-PPF) and by CYP3A4 to norpropafenone (NOR-PPF). CYP2D6 shows a high degree of genetic polymorphism which is associated with diminished antiarrhythmic efficacy or cardiac seizures/cardiotoxicity. This study aimed to investigate the effect of the CYP2D6 polymorphism on the pharmacokinetics of PPF and its two main metabolites.

In-vitro cytotoxicity and combination effects of the docetaxel-conjugated and doxorubicin-conjugated poly(lactic acid)-poly(ethylene glycol)-folate-based polymeric micelles in human ovarian cancer cells.

Objectives: The pH-sensitive doxorubicin (DOX)-conjugated and docetaxel (DTX)-conjugated poly(lactic acid)-poly(ethylene glycol)-folate (PLA-PEG-FOL)-based polymeric micelles were developed and characterized in this study.

Key Findings: The drugs were released from the micelles (particle size, ~185 nm) in a pH-dependent manner. The drug-conjugated PLA-PEG-FOL micelles showed higher cellular uptake than nontargeting ones.

Cloning, expression and purification of autolysin from methicillin-resistant Staphylococcus aureus: potency and challenge study in Balb/c mice.

Staphylococcus aureus (MRSA) is an opportunistic pathogen which causes a variety of clinical diseases and leads to high rates of morbidity and mortality. Development of an effective vaccine appears to be a useful strategy to control the infection. Here, the internal region of atl was cloned into the pET24a plasmid and expressed in E.