Combination of Two-Stage Continuous Feeding and Optimized Synthetic Medium Increases Lipid Production in .

The oleaginous yeast is recognized for its remarkable lipid accumulation under nitrogen-limited conditions. However, precise control of microbial lipid production in remains challenging due to the complexity of nutrient media. We developed a two-stage fed-batch fermentation process using a well-defined synthetic medium in a 5-L bioreactor.