1,068 results match your criteria: "Institute of applied Microbiology[Affiliation]"

Phytoremediation technology is viewed as a potential solution for addressing soil uranium contamination. Sudan grass ( (Piper) Stapf.), noted for its robust root structure and resilience to heavy metals, has garnered significant attention.

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Waterlogging is a significant stressor for crops, particularly in lowland regions where soil conditions exacerbate the problem. Waterlogged roots experience hypoxia, disrupting oxidative phosphorylation and triggering metabolic reorganization to sustain energy production. Here, we investigated the metabolic aspects that differentiate two soybean sister lines contrasting for waterlogging tolerance.

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Microbe-mediated synthesis of defect-rich CeO nanoparticles with oxidase-like activity for colorimetric detection of L-penicillamine and glutathione.

Nanoscale

January 2025

College of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, P.R. China.

To enhance production efficiency, curtail costs, and minimize environmental impact, developing simple and sustainable nanozyme synthesis methods has been the focus of relevant research. In this report, graphite-coated CeO nanoparticles (CeO NPs) with multiple defects (Ce defects, oxygen vacancies and carbon defects) were synthesized the culture filtrate of the extremely radioresistant bacterium R12 ( R12). The as-prepared CeO NPs exhibit remarkable oxidase (OXD)-like activity, efficiently catalyzing the oxidation of the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) to form oxTMB, even in the absence of HO.

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Essential to the determination of the ultimate and flavor of jujube jam are various processing stages. Nevertheless, the alterations in metabolites and flavor chemistry throughout the processing of jujube jam are poorly comprehended. This research employed metabolomics, flavor analysis, and microbial indicators to examine the impact of distinct processing stages on the metabolites and flavor profile of jujube jam.

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Background: Camels, known as the enduring "ships of the desert," host a complex gut microbiota that plays a crucial role in their survival in extreme environments. However, amidst the fascinating discoveries about the camel gut microbiota, concerns about antibiotic resistance have emerged as a significant global challenge affecting both human and animal populations. Indeed, the continued use of antibiotics in veterinary medicine has led to the widespread emergence of antibiotic-resistant bacteria, which has worsened through gene transfer.

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Enhanced butanol tolerance and production from puerariae slag hydrolysate by Clostridium beijerinckii through metabolic engineering and process regulation strategies.

Bioresour Technol

January 2025

College of Bioscience and Bioengineering, Institute of Applied Microbiology, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China. Electronic address:

Article Synopsis
  • Butanol is identified as a valuable second-generation biomass energy source, but its production faces challenges like toxicity to fermentation strains and high feedstock costs.
  • The study focuses on enhancing butanol tolerance and production in the strain Clostridium beijerinckii D9 through metabolic engineering, leading to the development of a more efficient strain, D9/pykA.
  • Results showed that the new strain achieved a significant increase in butanol and total solvent production compared to the original strain, with optimal conditions boosting yield and revealing mechanisms to improve fermentation efficiency.
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The chemical industry can now seize the opportunity to improve the sustainability of its processes by replacing fossil carbon sources with renewable alternatives such as CO, biomass, and plastics, thereby thinking ahead and having a look into the future. For their conversion to intermediate and final products, different types of catalysts-microbial, enzymatic, and organometallic-can be applied. The first part of this review shows how these catalysts can work separately in parallel, each route with unique requirements and advantages.

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Honey bees are exposed to a diverse variety of microbes in the environment. Many studies have been carried out on the microbiome of bee gut, beebread, and flower pollen. However, little is known regarding the microbiome of fresh corbicular pollen, which can directly reflect microbes acquired from the environment.

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Exploration of In Situ Extraction for Enhanced Triterpenoid Production by Saccharomyces cerevisiae.

Microb Biotechnol

December 2024

Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, Queensland, Australia.

Plant-derived triterpenoids are in high demand due to their valuable applications in cosmetic, nutraceutical, and pharmaceutical industries. To meet this demand, microbial production of triterpenoids is being developed for large-scale production. However, a prominent limitation of microbial synthesis is the intracellular accumulation, requiring cell disruption during downstream processing.

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Article Synopsis
  • Researchers studied the effects of GABA on improving lipid production in D. intermedius Z when exposed to furfural stress.
  • At 1.0 g/L furfural, growth nearly halted, but adding 1.5 mmol/L GABA resulted in significant increases in biomass and lipid production by 36.53% and 61.95%, respectively.
  • GABA helped maintain cell integrity and enhanced enzyme activities involved in fatty acid synthesis, suggesting it is a beneficial method for boosting microbial tolerance and performance in fermentation.
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  • The study examines the Siberian roe deer population in the Greater Khingan Mountains of Northeast China, emphasizing its role as an ecological indicator species and the importance of its living conditions for ecosystem balance.
  • Researchers collected and analyzed 269 fecal samples over three years using advanced genetic techniques, identifying 244 individual deer and revealing high genetic diversity within the population.
  • Despite the overall genetic health, the study indicated some populations are at risk, with evidence of genetic differentiation, potential inbreeding, and varying development potential among local deer populations.
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Sensitivities in protein allocation models reveal distribution of metabolic capacity and flux control.

Bioinformatics

November 2024

Institute of Applied Microbiology, Aachen Biology and Biotechnology, RWTH Aachen University, Aachen 52074, Germany.

Article Synopsis
  • The text discusses protein allocation models (PAMs) that improve predictions in metabolism by considering protein resource allocation, highlighting a lack of methods to analyze growth-limiting factors in these models.
  • A new framework called sEnz is introduced, which identifies sensitive enzyme concentrations in PAMs more efficiently than traditional methods, providing insights into factors that affect metabolic growth.
  • The sEnz framework is implemented in a Python toolbox called PAModelpy, which is available on Pypi and GitHub for further research and analysis.
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Solving the plastic crisis requires high recycling quotas and technologies that allow open loop recycling. Here a biological plastic valorization approach consisting of tandem enzymatic hydrolysis and monomer conversion of post-consumer polyethylene terephthalate into value-added products is presented. Hydrolysates obtained from enzymatic degradation of pre-treated post-consumer polyethylene terephthalate bottles in a stirred-tank reactor served as the carbon source for a batch fermentation with an engineered Pseudomonas putida strain to produce 90mg/L of the biopolymer cyanophycin.

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Genome-based analysis of biosynthetic potential from antimycotic Streptomyces rochei strain A144.

FEMS Microbiol Lett

January 2024

Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences/Xinjiang Key Laboratory of Special Environmental Microbiology, Urumqi 830091, PR China.

Streptomyces rochei is a species of Streptomyces with a diverse range of biological activities. Streptomyces rochei strain A144 was isolated from desert soils and exhibits antagonistic activity against several plant pathogenic fungi. The genome of S.

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Background: Biofilm formation on implant-abutment surfaces can cause inflammatory reactions. Ethical concerns often limit intraoral testing, necessitating preliminary in vitro or animal studies. Here, we propose an in vitro model using human saliva and hypothesize that this model has the potential to closely mimic the dynamics of biofilm formation on implant-abutment material surfaces in vivo.

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Downstream effects of the pandemic? Spatiotemporal trends of quaternary ammonium compounds in suspended particulate matter of German rivers.

J Hazard Mater

December 2024

Institute of Soil Science and Soil Conservation, Justus-Liebig University Gießen, Heinrich-Buff Ring 26, 35392 Gießen, Germany; Institute of Geography, Soil Science and Soil Resources, Ruhr University Bochum, Universitätsstraße 150, 44801 Bochum, Germany. Electronic address:

During the SARS-CoV-2 pandemic, the preventive use of antimicrobials such as quaternary ammonium compounds (QACs) surged worldwide. As cationic and surface-active biocides, QACs are only partly removed during wastewater treatment and may cause adverse ecological effects in the downstream environment. To understand the environmental consequences of increased disinfectant use during the pandemic, we investigated spatiotemporal QAC concentration trends in the suspended particulate matter (SPM) of three diverse German rivers.

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Pseudomonas sp. LFM046 produces polyhydroxyalkanoates of medium-chain length. When carbohydrates are used, only monomers of even-length chains (3HA) are generated.

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Background: Sponge-associated microorganisms are promising resources for the production of bioactive compounds with cytotoxic potential. The main goal of our study is to isolate the fungal endophytes from the Red Sea sponge Hyrtios sp. followed by investigating their cytotoxicity against number of cell lines.

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Using H16 to Provide a Roadmap for Increasing Electroporation Efficiency in Nonmodel Bacteria.

ACS Synth Biol

October 2024

Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Groningen 9713AV, The Netherlands.

Article Synopsis
  • Most industrial biotechnologies depend on a small number of established bacteria, limiting the potential of other nonmodel strains that have valuable metabolic capabilities.
  • This study focuses on improving electroporation efficiency in nonmodel bacteria, particularly H16, which can produce various chemicals but struggles with genetic manipulation due to low transformation rates.
  • The authors suggest techniques like using natively methylated DNA and bioinformatics to enhance cloning efficiency and successfully introduce genetic material, aiming to facilitate metabolic engineering in these bacteria.
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Unravelling abrupt alterations in the gut microbiota of wild species associated with nutritional stress is imperative but challenging for wildlife conservation. This study assessed the nutritional status of wild red deer during winter on the basis of changes in faecal nitrogen (FN) and urea nitrogen/creatinine (UN: C) levels and identified gut microbes associated with nutritional status via nutritional control experiments and metagenomic sequencing. The FN of wild red deer in winter 2022 was significantly lower than that in winter 2021 (p < 0.

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Protists, known as microeukaryotes, are a significant portion of soil microbial communities. They are crucial predators of bacteria and depend on bacterial community dynamics for the growth and evolution of protistan communities. In parallel, increased levels of atmospheric CO significantly impact bacterial metabolic activity in rhizosphere soils.

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Article Synopsis
  • Squalene is a natural compound found in various organisms, known for its antioxidant properties and ability to enhance skin penetration, making it valuable in cosmetics, food, and pharmaceuticals.
  • The primary source of squalene is sharks, leading to a growing demand for sustainable and eco-friendly alternatives for its production.
  • Recent advancements in biotechnology focus on utilizing microorganisms to efficiently synthesize squalene, with a review summarizing the latest strategies in metabolic and bioprocess engineering.
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State-of-the-Art Light-Driven Hydrogen Generation from Formic Acid and Utilization in Enzymatic Hydrogenations.

ChemSusChem

October 2024

Leibniz-Institut für Katalyse e. V. (LIKAT Rostock), Albert-Einstein-Str. 29a, 18059, Rostock, Germany.

Article Synopsis
  • A new method combines photocatalytic hydrogen generation with green enzymatic reductions, showing a significant increase in efficiency.
  • The system, using Pt(x)@TiO, produces hydrogen at levels much higher than existing technologies, based on a unique photocatalytic mechanism rather than thermal reactions.
  • The approach successfully synthesizes valuable compounds at low pressure and room temperature, indicating potential applications in flavors, fragrances, and pharmaceuticals.
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Integration of Fe-MOF-laccase-magnetic biochar: From Rational Designing of a biocatalyst to aflatoxin B1 decontamination of peanut oil.

Chemosphere

November 2024

Institute of Applied Microbiology, College of Agriculture, Guangxi University, Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, Nanning, 530004, China, Nanning, 530005, China. Electronic address:

Enzymatic degradation of aflatoxins in food commodities has gained significant attention. However, enzyme denaturation in organic media discourages their direct use in oils to remove aflatoxins. For that, enzymes are immobilized or encapsulated for improved stability and reusability under unfavorable conditions.

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Background: Komagataella phaffii (K. phaffii), formerly known as Pichia pastoris, is a widely utilized yeast for recombinant protein production. However, due to the formation of overflow metabolites, carbon yields may be reduced and product recovery becomes challenging.

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