[Effect of nucleosomal binding protein 1 in androgen-independent prostatic carcinoma].

Objective: To investigate biological function of nucleosomal binding protein 1 (NSBP1) in androgen-independent prostate cancer cells.

Methods: Androgen-independent prostate cancer cells of the line PC-3 were cultured and transfected with recombinant inhibiting siRNA expression plasmid pSilencer 2. 1-U6 neo.

Deficit of mitochondria-derived ATP during oxidative stress impairs mouse MII oocyte spindles.

Although the role of oxidative stress in maternal aging and infertility has been suggested, the underlying mechanisms are not fully understood. The present study is designed to determine the relationship between mitochondrial function and spindle stability in metaphase II (MII) oocytes under oxidative stress. MII mouse oocytes were treated with H2O2 in the presence or absence of permeability transition pores (PTPs) blockers cyclosporin A (CsA).

Redox-induced apoptosis of human oocytes in resting follicles in vitro.

Background: The age-related depletion of the resting follicle (RF) stock occurs as a result of two processes: atresia and entry in growth phase. Due to difficulties in obtaining sufficient numbers of RFs for study, little is known about the apoptotic mechanisms for RF atresia. The present study was designed to investigate the effects of oxidative stress on the apoptosis of RF oocytes.

[Further studies on the relationship between benign prostatic hyperplasia and prostatitis].

Benign prostatic hyperplasia(BPH) and prostatitis are two common diseases in aging men. In recent years, the study of correlation between these two diseases has been paid more and more attention to. Some researchers presumed that inflammation is one of the most important causations of BPH, however, it is not profound enough on this etiologic hypothesis at present.

[Multivariate analysis of recurrence in T1 bladder transitional cell carcinoma].

Objective: To investigate the prognostic value of some clinicopathologic indexes and biologic tumor markers in predicting recurrence in T1 transitional cell carcinoma (TCC) of the bladder.

Methods: The expressions of p53, E-cadherin and VEGF of 75 patients with T1 primary bladder TCC were detected by streptabitin peroxidase (SP) immunohistochemical methods. The effects of clinicopathologic indexes and biologic tumor markers on recurrence were assessed by Kaplan-Meier and Cox proportional hazards model.