[EATING DISORDERS AND VISCERAL ADIPOSE TISSUE - TWO INTERCONNECTED INFORMATIVE MARKERS OF PROGNOSIS OF DISORDERS OF NUTRITIONAL STATUS AND RISK OF DEVELOPMENT OF COMORBIDITY CHRONIC NON-COMMUNICABLE DISEASES].

The purpose of research was to study the characteristics of eating behaviour (EB) and indicators visceral adipose tissue (VAT) in patients with NAFLD and obesity stage of 1-2 or excessive body mass on the background of hypertension (H). We examined 100 patients with NAFLD in combination with overweight and obesity of 1-2 degrees (body mass index - BMI ≥25 kg/m2) on the background of H I-II stage. Patients with NAFLD and visceral obesity on the background of stage H of I-II the identified three types of violations of EB with a significant predominance of external breach type EB (p<0,05) regardless of BMI.

[Combination therapy of chronic bacterial prostatitis].

The article discusses the possible etiological factors in the development of chronic bacterial prostatitis. The authors presented a comparative long-term analysis of morbidity from non-viral sexually transmitted infections (STIs) in Russia. Against the background of general decline in STIs incidence, a significant percentage of them is made up by urogenital trichomoniasis.

Frequency of 3' VNTR Polymorphism in the Dopamine Transporter Gene SLC6A3 in Humans Predisposed to Antisocial Behavior.

Predisposition to antisocial behavior can be related to the presence of certain polymorphic variants of genes encoding dopaminergic system proteins. We studied the frequencies of allele variants and genotypes of variable number tandem repeat polymorphism in 3' untranslated region (3' VTNR) of the dopaminergic transporter SLC6A3 gene in Caucasian men committed socially dangerous violent and non-violent crimes. Alleles with 9 and 10 repeats were most frequent in both the control group and group of men predisposed to antisocial behavior.

THE ROLE OF POLYMORPHISM - 634 G/C (RS 2010963) OF VEGF-A GENE IN THE DEVELOPMENT OF HYPERTENSION AND OBESITY IN PREMENOPAUSAL WOMEN.

The study of genetic dependency of developing cardiovascular diseases and factors of endothelial dysfunction in menopausal women is extremely important in today's world. The aim of the study was to assess the correlation of polymorphic marker -634G/С (rs2010963) of the VEGF-А gene and formation of AH combined with obesity in premenopausal women. The data of 115 women with stage II of AH, grade 1-2 and obesity I-II gr.

CORRELATION OF HELICOBACTER PYLORI INFECTION WITH DEVELOPMENT OF CARDIOVASCULAR RISK IN PATIENTS WITH CORONARY HEART DISEASE IN ASSOCIATION WITH TYPE 2 DIABETES MELLITUS.

Recent years have demonstrated the possible existence of association between infection Helicobacter pylori (HP), coronary heart disease (CHD) and type 2 diabetes mellitus (DM-2). The refinement of traditional and non-traditional factors of cardiovascular risk in patients with CHD in association with DM-2 of HP-seropositiveis currently important. In this regard, we have studied the influence of HP infection on cardiovascular risk in patients with CHD and DM-2.

[Diagnosis and treatment of psychogenic dizziness in patients with arterial hypertension].

Objective: To analyze the prevalence, methods of diagnosis and treatment of psychogenic dizziness in patients with arterial hypertension and evaluate the efficacy of treatment with noophen and betahistin.

Material And Methods: Fifty-eight patients were studied. Patients of the main group received noophen and patients of the comparison group were treated with betahistin.

[FEATURES MANIFESTATIONS COMORBID CURRENTS OF GASTROESOPHAGEAL REFLUX DISEASE AND ARTERIAL HYPERTENSION DEPENDING ON THE DEGREE OF ARTERIAL HYPERTENSION].

The purpose of research - to study the features of circadian profile of blood pressure (BP), the data of echocardiography, pH-monitoring, lipid spectrum, level of apelin and the state of the system "lipid peroxidation-antioxidant protection" in patients with a combination of arterial hypertension (AH) and gastroesophageal reflux disease (GERD) depending on the level of AH. It was examined 126 patients with combination of AH II stage, 2-3 degrees, and GERD, 70 (55.56%) men and 56 (44.

[A role of treatment of autonomic syndrome in patients with tension-type headache].

Unlabelled: The study aims to evaluate the effect of correction of psycho-vegetative violations anxiolitikami on the frequency and intensity of headache.

Material And Methods: the study included 50 women (average age - 37,4 years) with confirmed diagnosis: frequent episodic headache and chronic headache no factor abuses. Patients of the main group received ibuprofen to 200-400 mg/day for headaches in combination with tizanidine (2-4 mg/day) and anvifen 750 mg a day for 8 weeks.

[Workplace stress and its impact on the 16-year risk of myocardial infarction and stroke in an open female population aged 25-64 years in Russia/Siberia (WHO MONICA-psychosocial program)].

Aim: To determine the impact of workplace stress on the hazard ratio (HR) of myocardial infarction (M) and stroke in an open female population aged 25-64 years in Russia/Siberia (Novosibirsk) for 16 years.

Subjects And Methods: A random representative sample of 25-64-year-old women (n=870) residing in a Novosibirsk district was surveyed within the framework of the WHO <> program. Workplace stress was investigated using the Karasek scale; an attitude towards work and health prophylactic examinations was studied applying the Health Awareness and Attitude questionnaire of the WHO <> program.

[Significance of surfactant proteins in the diagnosis of therapeutic diseases].

The lung provides not only respiration, but also the functioning of innate immunity mechanisms. The hydrophilic proteins SP-A and SP-D are responsible for the regulation of the latter. In the literature, there is evidence for elevated serum SP-A and SP-D levels in respiratory diseases accompanied by enhanced mucosal inflammation of the lung or its parenchymal injury and their association with age and cardiovascular diseases has been recently found.

[Gender differences in health awareness and attitudes as a subjective-objective health index in the population of Russia/Siberia (WHO MONICA-psychosocial program, HAPIEE project)].

Aim: To establish gender differences in health attitudes and awareness of risk factors for cardiovascular diseases in an open 25-64-year-old population of Russia/Siberia.

Subjects And Methods: A representative sample from the population of a Novosibirsk district was examined using the 1988 WHO MONICA-MOPSY (847 women and 739 men aged 25-64 years) and the 2003 HAPIEE (1074 women and 576 men aged 45-64 years) programs. The health awareness and attitudes questionnaire was used.

Possible role and application of fMRI in the screening of release-active drugs.

Functional magnetic resonance imaging (fMRI) of the brain was applied for preclinical evaluation of the efficiency of Divaza preparation intended for the treatment of cerebrovascular disorders. Psychological testing (Stroop task) in the magnetic field of fMRI was performed before and after 12-week treatment course using a double blind placebo-controlled protocol. It was shown that standard psychological and neuropsychological protocols do not allow fully estimate the results of treatment, whereas fMRI targeted the pool of cerebral structures activated during task solution.

The state of coronary arteries in perimenopausal women with chest pain.

Background: The goal of the study was to assess the state of coronary arteries in perimenopausal women undergoing examination before prescription of hormonal replacement therapy.

Methods: One hundred ninety-three patients were screened, and 43 of them were selected for inclusion to the study. Pretest probability of coronary heart disease (CHD) was 47% for patients with typical angina pain and 20.

CETP gene polymorphism in the caucasian population of West Siberia and in groups contrast by total serum cholesterol levels.

TaqIB polymorphism of the gene encoding cholesterol ester transporting protein (CETP) was analyzed in the Caucasian population of West Siberia and in groups contrast by total serum cholesterol content. The patients were selected for the study from the main sample of HAPIEE project (9600 examined subjects aged 45-69 years, 50% men). Analysis was carried out in 293 patients with high levels of total cholesterol (>300 mg/dl), 293 patients with normal and low levels of total cholesterol (<200 mg/dl), and 265 patients represented the population sample (mean level of total cholesterol 235.

Effects of simvaglyzin and atorvaglyzin on the expression of 3-hydroxy-3-methyl-glutaryl-CoA reductase in rat liver.

Activity and levels of protein and mRNA of 3-hydroxy-3-methyl-glutaryl-CoA reductase were estimated in rat liver after the administration of atorvastatin and simvastatin and their complexes with glycyrrhizic acid (atorvaglyzin and simvaglyzin). The amount of 3-hydroxy-3-methyl-glutaryl-CoA reductase protein in rats decreased by 13 and 25% (p<0.05) 24 h after treatment with atorvaglyzin and simvaglyzin, respectively.

Effect of toll-like receptor agonists on the formation of macrophage/foam cells upon acute peritonitis in mice.

We studied effects of zymosan, double-stranded RNA, LPS of E. coli and bacterial CpG DNA, agonists of toll-like receptor TLR2, TLR3, TLR4 and TLR9, respectively, on the formation of macrophage/foam cells 24 h after induction of acute peritonitis. Administration of agonists led to transformation of peritoneal macrophages into foam cells and significant activation of cell biosynthesis and increased the content of triglycerides and cholesterol esters in the absence of LDL and irrespective of the capacity of TLR agonists to stimulate neutrophil infiltration and TNF-α production in the peritoneal cavity.

Bezafibrate causes depression of the immune response and increases the sensitivity to endotoxin in association with low level of HDL and PPARα activity in hypertensive ISIAH rats.

We studied the effect of bezafibrate on hepatic PPARα activity and immune parameters in hypertensive ISIAH rats in comparison with normotensive WAG rats under conditions of LPS treatment. Bezafibrate increased activity of PPARα in WAG rats, but not in ISIAH rats. As differentiated from WAG rats, bezafibrate produced a potent effect on the content of T cell subpopulations in the thymus and spleen of ISIAH rats.

Reactivity of the immune system and activities of PPARα and PPARγ in the liver under conditions of LPS-induced acute inflammatory response in hypertensive ISIAH rats.

The effects of LPS from E. coli on DNA-binding activities of PPARα and PPARγ in the liver and immune system parameters of were studied in hypertensive ISIAH rats and normotensive WAG rats. In ISIAH rats characterized by low basal level of PPARα, PPARγ, and HDL, the response of the peripheral immune system compartment to LPS was more pronounced and was not associated with decrease in DNA-binding activities of PPARα observed in WAG.

Relationship of blood levels of inflammatory and destructive biomarkers in coronary atherosclerosis with long-term results of surgical revascularization.

The relationships between blood levels of inflammatory and destructive biomarkers (TNF-α, IL-1β, IL-6, IL-8, soluble CD40 ligand, high-sensitivity C-reactive protein, endothelial adhesion molecules, matrix metalloproteinase 3 and 9 and their tissue inhibitor type 1) were studied in men with coronary atherosclerosis before and 5 years after coronary artery bypass surgery. In men with unfavorable course of coronary atherosclerosis in the delayed period, the initial blood levels of C-reactive protein and TNF-α by 1.7 and 3.

[Intronic polymorphisms of аntionkogene TP53 in patients with indolent variants of Non-Hodgkin's lymphomas].

The frequency of distribution of alleles and genotypes of single nucleotide substitution G13494A in intron 6 and duplications dup16bp in intron 3 of the gene TP53 in 56 patients with indolent non-Hodgkin's lymphoma and variants in the controls was studied. The increase of the frequency of G-allele and G/G genotype of intron 6 of the gene TP53 in lymphoma patients compared with controls (91 and 84 % vs. 79 (p<0,01) and 63 % (p<0,01), respectively) was determined.

Oxidation and endothelial dysfunction biomarkers of atherosclerotic plaque instability. Studies of the vascular wall and blood.

The concentrations of LPO products (including those present in LDL), oxidative modification of proteins, paraoxonase activity, concentrations of antioxidants, lipid values and biomarkers of endothelial dysfunction were studied in the blood and coronary artery intima/media of male patients with coronary atherosclerosis without acute coronary syndrome. Blood levels of LDL oxidized apolipoproteins and lipoprotein (a) were higher, while the content of NO metabolites, sVCAM endothelial adhesion molecules, and LDL oxidation resistance were lower in men with mainly unstable atherosclerotic plaques in the coronary arteries in comparison with men with mainly stable plaques in the coronary arteries. Of these blood biomarkers, only NO metabolites, oxidized proteins, and sVCAM correlated with the presence of unstable atherosclerotic plaques.

Activity of the inflammatory process in different types of unstable atherosclerotic plaques.

Inflammatory biomarkers and chemoattractants characteristic and important for different types (lipid; inflammatory erosive; degenerative necrotic) of unstable plaques in coronary arteries were identified and studied in male patients with coronary atherosclerosis without acute coronary syndrome we studied. Among the three types of unstable plaques, elevated concentrations of IL-1β, IL-6, IL-8, IL-18 and monocyte chemotactic protein-1 were characteristic of not only inflammatory erosive type, but also lipid type compared with degenerative necrotic type. Thus, intensification of the inflammatory process plays an important role in the development of not only inflammatory and destructive, but also of lipid type of unstable atherosclerotic plaques.

Blood level of osteonectin in stenosing atherosclerosis and calcinosis of coronary arteries.

Blood levels of stem cell marker proteins CD34 and osteonectin were studied in male patients with coronary atherosclerosis by direct biomagnetic separation of proteins with magnetic microspheres using the PureProteome Protein A and Protein G Magnetic Beads proteomic technology. High concentration of osteonectin in the blood was detected, particularly in men with stenosing atherosclerosis and coronary artery calcinosis. Blood osteonectin concentration correlated significantly with some key biomarkers of atherosclerosis and with stenosing atherosclerosis and calcinosis of coronary arteries.

Apolipoprotein E gene polymorphism in men with coronary atherosclerosis in Siberia.

Polymorphism of coding fragment of APOE gene was analyzed in two groups of men. The main group consisted of 77 residents of the West Siberian region aged 45-65 years with coronary atherosclerosis (documented by coronary angiography) without acute coronary syndrome with stable effort angina, functional class II-IV. The reference group consisted of 350 residents of Novosibirsk, aged 45-69 years.

Effects of cholesterol and nuclear hormone receptor agonists on the production of transforming growth factor-beta in macrophages.

We studied the effects of cholesterol, its oxidized derivatives mevalonate, and nuclear receptor agonists LXR, RXR, and FXR on the production of transforming growth factor-beta1 (TGF- beta1) by macrophages. After recruiting of macrophage monocytes into the focus of inflammation, the production of TGF-beta1 increased by 3.5 times in comparison with control macrophages.