Genomic diversity and potential transmission and persistence of Salmonella in the Cambodian vegetable supply chain.

S. enterica isolates (n = 78) obtained from the vegetable supply chain (farms, distribution centers, markets) in two Cambodian provinces (Siem Reap, Battambang) were sequenced and analyzed. In silico identification of serotypes and detection of antimicrobial resistance genes was performed using SISTR and ABRicate, respectively.

The transport and vertical distribution of microplastics in the Mekong River, SE Asia.

Rivers are primary vectors of plastic debris to oceans, but sources, transport mechanisms, and fate of fluvial microplastics (<5 mm) remain poorly understood, impeding accurate predictions of microplastic flux, ecological risk and socio-economic impacts. We report on microplastic concentrations, characteristics and dynamics in the Mekong River, one of the world's largest and polluting rivers, in Cambodia and Vietnam. Sampling throughout the water column at multiple localities detected an average of 24 microplastics m (0.

Comparison of plant microbiota in diseased and healthy rice reveals methylobacteria as health signatures with biocontrol capabilities.

Introduction: Rice () is a staple food worldwide, but its production is under constant pressure from both abiotic and biotic stresses, resulting in high use of agrochemicals. The plant microbiome harbours microorganisms that can benefit plant health and provide alternatives to the use of agrochemicals. The composition of plant microbiomes depends on many factors (soil composition, age, and health) and is considered a primary driver of future plant health.

Evaluating the impact of climate change on yield and water use efficiency of different dry-season rice varieties cultivated under conventional and alternate wetting and drying conditions.

This study is the first attempt to assess rice cultivation under alternate wetting and drying (AWD) and continuous flooding (CF) using the latest scenarios from the Intergovernmental Panel on Climate Change (IPCC), utilizing AquaCrop Model. Field experiments were conducted during the dry season 2023 to get the model calibration and validation input. We used two shared socioeconomic pathways scenarios (SSP3-7.

Response of bacterial communities and soil chemistry to flood durations and recovery phases.

Flooding profoundly impacts soil bacterial communities; however, the underlying mechanisms remain poorly understood. This study investigated how flooding (3, 8, and 16 days) and post-flooding (immediately and 2, 5, and 30 days) durations affect soil physicochemical properties, bacterial communities, and their interactions-crucial factors in floodplain nutrient and carbon cycling. The results showed that bacteria constituted 99.

Unraveling urban hydro-environmental response to climate change and MCDA-based area prioritization in a data-scarce developing city.

Climate change leads to more frequent and intense heavy rainfall events, posing significant challenges for urban stormwater management, particularly in rapidly urbanizing cities of developing countries with constrained infrastructure. However, the quantitative assessment of urban stormwater, encompassing both its volume and quality, in these regions is impeded due to the scarcity of observational data and resulting limited understanding of drainage system dynamics. This study aims to elucidate the present and projected states of urban flooding, with a specific emphasis on fecal and organic contamination caused by combined sewer overflow (CSO).

Antibiotic resistance profiles of sentinel bacteria isolated from aquaculture in Cambodia.

The misuse of antibiotics and the emergence of antimicrobial resistance (AMR) is a concern in the aquaculture industry because it contributes to global health risks and impacts the environment. This study analyzed the AMR of sentinel bacteria associated with striped catfish () and giant snakehead (), the two main fish species reared in the pond culture in Cambodia. Phenotypic and genotypic characterization of the recovered isolates from fish, water, and sediment samples revealed the presence of bacteria, such as 22 species belonging to families , , and .

Plastic pollution affects ecosystem processes including community structure and functional traits in large rivers.

Plastics in aquatic ecosystems rapidly undergo biofouling, giving rise to a new ecosystem on their surface, the 'plastisphere.' Few studies quantify the impact of plastics and their associated community on ecosystem traits from biodiversity and functional traits to metabolic function. It has been suspected that impacts on ecosystems may depend on its state but comparative studies of ecosystem responses are rare in the published literature.

Can restoring water and sediment fluxes across a mega-dam cascade alleviate a sinking river delta?

Hydropower, although an attractive renewable energy source, can alter the flux of water, sediments, and biota, producing detrimental impacts in downstream regions. The Mekong River illustrates the impacts of large dams and the limitations of conventional dam regulating strategies. Even under the most optimistic sluicing scenario, sediment load at the Mekong Delta could only recover to 62.

Storage and dynamics of soil organic carbon in allochthonous-dominated and nitrogen-limited natural and planted mangrove forests in southern Thailand.

Mangrove forests can help to mitigate climate change by storing a significant amount of carbon (C) in soils. Planted mangrove forests have been established to combat anthropogenic threats posed by climate change. However, the efficiency of planted forests in terms of soil organic carbon (SOC) storage and dynamics relative to that of natural forests is unclear.

Photodegradation of biodegradable plastics in aquatic environments: Current understanding and challenges.

Direct and indirect photolysis are important abiotic processes in aquatic environments through which plastics can be transformed physically and chemically. Transport of biodegradable plastics in water is influenced by vertical mixing and turbulent flow, which make biodegradable plastics remain susceptible to sunlight and photolysis despite their high density. In general, biodegradable plastics are composed of ester containing polymers (e.

Tracing the pathways by which flood duration impacts soil bacteria through soil properties and water-extractable dissolved organic matter: A soil column experiment.

Soil microbial communities control biogeochemical processes, nutrient cycling, and organic carbon storage and release in wetlands, which are influenced by flooding. To predict soil nutrient function in wetland ecosystems, understanding the effect of flooding on soil biogeochemical cycling and energy flux, including soil properties, dissolved organic matter (DOM), and microbial communities is essential. This study investigated how different flood durations (1, 3, 8, 16, and 30 d) affect the interactions between physicochemical properties and bacterial communities in a river wetland.

High methoxyl pectin can improve the extrusion characteristics and increase the dietary fiber content of starch-cellulose extrudates.

Improving total dietary fiber content while maintaining the texture/expansion of extruded products is a challenge. Pectin has a dual function; it is a source of dietary fiber and it also functions as a hydrocolloid, which could improve the texture of high-fiber extruded foods. The objective of this study was to evaluate the impacts of pectin types from citrus peel on the expansion characteristics of starch-cellulose extrudates.

Fish biodiversity declines with dam development in the Lower Mekong Basin.

Hydropower dams are a source of renewable energy, but dam development and hydropower generation negatively affect freshwater ecosystems, biodiversity, and food security. We assess the effects of hydropower dam development on spatial-temporal changes in fish biodiversity from 2007 to 2014 in the Sekong, Sesan, and Srepok Basins-major tributaries to the Mekong River. By analyzing a 7-year fish monitoring dataset, and regressing fish abundance and biodiversity trends against cumulative number of upstream dams, we found that hydropower dams reduced fish biodiversity, including migratory, IUCN threatened and indicator species in the Sesan and Srepok Basins where most dams have been constructed.

Does drying preserve the nutritional quality of small freshwater fish without excessive concentrations of heavy metals?

The potential of drying to preserve the nutritional quality of a small freshwater fish was assessed. Drying time to reach moisture content and water activity of 10 g/100g and 0.65 ranged from 55 h at 50 °C to 20 h at 80 °C, respectively.

The Development of a Digital Twin to Improve the Quality and Safety Issues of Cambodian Pâté: The Application of 915 MHz Microwave Cooking.

Foodborne diseases are common in Cambodia and developing good food hygiene practices is a mandatory goal. Moreover, developing a low-carbon strategy and energy efficiency is also a priority. This study focuses on pâté cooking, a very common food product in Cambodia.

Influences of modified fiber inclusion with varying particle size on corn starch-based extrudate expansion.

The inclusion of cellulose nanocrystals (CNC) and microcrystalline cellulose (MCC) during extrusion processing of corn starch (CS) is presented in this study. Blends were prepared by incorporating CNC and MCC at different concentrations, 1%, 3%, 5%, and 10% w/w in CS. The crystallinity index (CrI) of CNC and MCC was determined using X-ray diffraction, and the chemical functionality of CNC, MCC, and CS was studied using Fourier transform infrared spectroscopy.

Integrated assessment of climate change and reservoir operation on flow-regime and fisheries of the Sekong river basin in Lao PDR and Cambodia.

This study assesses the cumulative impact of climate change and reservoir operation on flow regime and fisheries in the Sekong River Basin. Ensemble of five selected Regional Climate Models (RCMs) were used to project the future climate under RCP4.5 and RCP8.

Dissolved silicon in a lake-floodplain system: Dynamics and its role in primary production.

Dissolved silicon (DSi) is essential for aquatic primary production and its limitation relative to nitrogen (N) and phosphorus (P) facilitates cyanobacterial dominance. However, the effects of DSi on phytoplankton growth and community structure have yet to be fully determined in tropical lakes, particularly in relation to N and P. Therefore, this study investigated the role of DSi in Tonlé Sap Lake, Cambodia, a tropical floodplain system well known for its flood-pulse characteristics and high productivity.

Extrusion characteristics of ten novel quinoa breeding lines.

Ten novel breeding lines of quinoa (Chenopodium quinoa Willd) suitable to be grown in the Pacific Northwest of the United States were developed and utilized for extrusion processing. Understanding how a particular breeding line performs during food processing and which properties determine its performance can promote the use of quinoa as an ingredient in value-added products, such as extruded snacks. In this study, extrusion characteristics of the whole seed flours of the novel quinoa breeding lines were evaluated using a co-rotating twin-screw extruder at two temperatures (110°C and 125°C), three screw speeds (200, 350, and 500 rpm), and a moisture content of 18% (w.

Detection and genetic analysis of Escherichia coli from Tonle Sap Lake and its tributaries in Cambodia: Spatial distribution, seasonal variation, pathogenicity, and antimicrobial resistance.

As an indicator of fecal contamination, Escherichia coli was monitored in Tonle Sap Lake, Cambodia, and its tributaries during low- and high-water seasons, focusing on the impacts on floating villagers inhabiting boathouses. E. coli concentrations in the floating villages (3.

Prediction of mining-induced subsidence at Barapukuria longwall coal mine, Bangladesh.

It is essential to predict the mining-induced subsidence for sustainable mine management. The maximum observed subsidence having a noticeable areal extent due to Northern Upper Panels (NUP) and Southern Lower Panels (SLP) at the Barapukuria longwall coal mine is 5.8 m and 4.