66 results match your criteria: "Institute of Technical Biocatalysis[Affiliation]"
Sci Rep
June 2024
Department of Biochemistry, Microbiology and Biotechnology, Yerevan State University, Alex Manoogian 1, 0025, Yerevan, Armenia.
In the field of biotechnology, the utilization of agro-industrial waste for generating high-value products, such as microbial biomass and enzymes, holds significant importance. This study aimed to produce recombinant α-amylase from Anoxybacillus karvacharensis strain K1, utilizing whey as an useful growth medium. The purified hexahistidine-tagged α-amylase exhibited remarkable homogeneity, boasting a specific activity of 1069.
View Article and Find Full Text PDFBioresour Bioprocess
May 2024
Institute of Biotechnology, RWTH Aachen University, Aachen, Germany.
Extremophiles
April 2024
Biology Department and RI-Bio3, National and Kapodistrian University of Athens, Athens, Greece.
Extremophiles and their products have been a major focus of research interest for over 40 years. Through this period, studies of these organisms have contributed hugely to many aspects of the fundamental and applied sciences, and to wider and more philosophical issues such as the origins of life and astrobiology. Our understanding of the cellular adaptations to extreme conditions (such as acid, temperature, pressure and more), of the mechanisms underpinning the stability of macromolecules, and of the subtleties, complexities and limits of fundamental biochemical processes has been informed by research on extremophiles.
View Article and Find Full Text PDFMolecules
December 2023
Institute of Technical Biocatalysis, Hamburg University of Technology, 21073 Hamburg, Germany.
The fixation of CO by enzymatic carboxylation for production of valuable carboxylic acids is one way to recycle carbon. Unfortunately, this type of reaction is limited by an unfavourable thermodynamic equilibrium. An excess of the C1 substrate is required to increase conversions.
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January 2024
Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg, Germany.
The occurrence of organically bound phosphorus (P) as phytate in plant-based feeding material is a challenge for livestock farming due to limited utilization during the digestion by the animal. Its excretion into the environment through the manure pathway, poses a challenge, due to increased eutrophication and restrictions for P. Hence, while the routine supplementation of phytase enzymes in monogastric diets is common practice, metabolically triggering endogenous plant enzymes by wet-treatment prior to feeding can also lead to a better utilization of phytate bound P and increased digestibility by the animal.
View Article and Find Full Text PDFExtremophiles
November 2023
Institute of Technical Biocatalysis, Center for Biobased Solutions, Hamburg University of Technology, Am Schwarzenberg-Campus 4, 21073, Hamburg, Germany.
The development of sustainable and environmentally friendly industrial processes is becoming very crucial and demanding for the rapid implementation of innovative bio-based technologies. Natural extreme environments harbor the potential for discovering and utilizing highly specific and efficient biocatalysts that are adapted to harsh conditions. This review focuses on extremophilic microorganisms and their enzymes (extremozymes) from various hot springs, shallow marine vents, and other geothermal habitats in Europe and the Caucasus region.
View Article and Find Full Text PDFN Biotechnol
December 2023
Institute of Technical Biocatalysis, Hamburg University of Technology, Denickestraße 15, 21073 Hamburg, Germany. Electronic address:
The electroenzymatic hydroxylation of 4-ethylbenzoic acid catalyzed by the recombinant unspecific peroxygenase from the fungus Agrocybe aegerita (rAaeUPO) was performed in a gas diffusion electrode (GDE)-based system. Enzyme stability and productivity are significantly affected by the way the co-substrate hydrogen peroxide (HO) is supplied. In this study, two in-situ electrogeneration modes of HO were established and compared.
View Article and Find Full Text PDFMolecules
September 2023
Institute of Technical Biocatalysis, Hamburg University of Technology, Denickestraße 15, 21073 Hamburg, Germany.
Ulvan is a sulfated polysaccharide extracted from green macroalgae with unique structural and compositional properties. Due to its biocompatibility, biodegradability, and film-forming properties, as well as high stability, ulvan has shown promising potential as an ingredient of biopolymer films such as sustainable and readily biodegradable biomaterials that could replace petroleum-based plastics in diverse applications such as packaging. This work investigates the potential of as a source of ulvan.
View Article and Find Full Text PDFPolymers (Basel)
June 2023
Institute of Technical Biocatalysis, Hamburg University of Technology, 21073 Hamburg, Germany.
Epoxy resins are highly valued for their remarkable mechanical and chemical properties and are extensively used in various applications such as coatings, adhesives, and fiber-reinforced composites in lightweight construction. Composites are especially important for the development and implementation of sustainable technologies such as wind power, energy-efficient aircrafts, and electric cars. Despite their advantages, their non-biodegradability raises challenges for the recycling of polymer and composites in particular.
View Article and Find Full Text PDFAppl Microbiol Biotechnol
July 2023
Hamburg University of Technology, Institute of Technical Biocatalysis, Hamburg, Germany.
Side streams of the dairy industry are a suitable nutrient source for cultivating microorganisms, producing enzymes, and high-value chemical compounds. The heterotrophic Escherichia coli and chemolithoautotroph Ralstonia eutropha are of major biotechnological interest. R.
View Article and Find Full Text PDFJ Environ Manage
October 2023
Circular Resource Engineering and Management (CREM), Institute of Environmental Technology and Energy, Economics, Hamburg University of Technology, Blohmstr. 15, 21079, Hamburg, Germany.
Despite the increased demand for resource recovery from spent lithium-ion batteries (LIBs), low Mn leaching efficiencies have hindered the development of this technology. A novel process was devised to enhance the dissolution of metals by producing citric acid using a molasses medium by Penicillium citrinum. This investigation used response surface methodology to investigate the influence of molasses concentration and media components on citric acid production, which demonstrated that molasses (18.
View Article and Find Full Text PDFBeilstein J Org Chem
May 2022
Institute of Technical Biocatalysis, Hamburg University of Technology, Denickestr. 15, 21073 Hamburg, Germany.
The importance of a compound that helps fight against influenza is, in times of a pandemic, self-evident. In order to produce these compounds in vast quantities, many researchers consider continuous flow reactors in chemical industry as next stepping stone for large scale production. For these reasons, the synthesis of -acetylneuraminic acid (Neu5Ac) in a continuous fixed-bed reactor by an immobilized epimerase and aldolase was investigated in detail.
View Article and Find Full Text PDFAppl Microbiol Biotechnol
April 2022
Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.
The Tsukamurella tyrosinosolvens PS2 strain was isolated from hydrocarbons-contaminated petrochemical sludge as a long chain alkane-utilizing bacteria. Complete genome analysis showed the presence of two alkane oxidation systems: alkane 1-monooxygenase (alkB) and cytochrome P450 monooxygenase (P450) genes with established high homology to the well-known alkane-degrading actinobacteria. According to the comparative genome analysis, both systems have a wide distribution among environmental and clinical isolates of the genus Tsukamurella and other members of Actinobacteria.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
January 2022
Environmental Assessment and Technology for Hazardous Waste Management Research Center, Faculty of Environmental Management, Prince of Songkla University, Songkhla, 90112, Thailand.
The dephenolization of palm oil mill effluent (POME) with oil palm fiber-immobilized Trametes hirsuta AK 04 was conducted in a temporary immersion bioreactor to reduce the inhibitory effects of phenolics in anaerobic digestion. Longer immersion times provided greater removal of phenolics due to a higher release of manganese peroxidase. The most effective dephenolization was observed at 6 h immersed and 2 h non-immersed time (immersion ratio 6/8) with maximum removal of 85% from 1277 mg L of phenolics in 4 days.
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January 2021
Institute of Technical Biocatalysis, Hamburg University of Technology Hamburg Germany
The present study focuses on the aeration of aqueous triethanolamine acting as reaction medium for biocatalytic carboxylations. For enhancing mass transfer in a bubble column reactor, microbubble aeration is applied and compared to conventional macrobubble aeration. Application of a 0.
View Article and Find Full Text PDFOrg Biomol Chem
October 2020
Institute of Technical Biocatalysis, Hamburg University of Technology, Denickestr. 15, 21073 Hamburg, Germany.
Fatty alcohols are important products in chemical industry to be used in the formulation of surfactants and lubricants. This work describes a two step approach for the production of myristyl alcohol under neat conditions by combining a lipase catalyzed esterification of myristic acid and myristyl alcohol with a ruthenium catalyzed hydrogenation of the intermediate myristyl myristate. The esterification was carried out in a bubble column reactor with the commercial immobilized lipase B from Candida antarctica as a biocatalyst, while the hydrogenation was conducted under pressurized conditions being catalyzed by the homogeneous chemocatalyst Ru-Macho-BH.
View Article and Find Full Text PDFAnal Methods
June 2020
Hamburg University of Technology, Institute of Technical Biocatalysis, Hamburg, Germany.
In this study, an inline analytical method was designed and applied in process characterisation and development. The model reaction is the two-step diesterification of 3-methylphthalic anhydride with 2-ethylhexanol consisting of alcoholysis as the first step, followed by an acid-catalysed, second esterification step leading to the corresponding diester. The final product is a potential, alternative plasticiser.
View Article and Find Full Text PDFBiotechnol Bioeng
January 2021
Hamburg University of Technology, Institute of Technical Biocatalysis, Hamburg, Germany.
The sufficient provision of oxygen is mandatory for enzymatic oxidations in aqueous solution, however, in process optimization this still is a bottleneck that cannot be overcome with the established methods of macrobubble aeration. Providing higher mass transfer performance through microbubble aerators, inefficient aeration can be overcome or improved. Investigating the mass transport performance in a model protein solution, the microbubble aeration results in higher k a values related to the applied airstream in comparison with macrobubble aeration.
View Article and Find Full Text PDFJ Environ Sci Health A Tox Hazard Subst Environ Eng
July 2020
Environmental Assessment and Technology for Hazardous Waste Management Research Center, Faculty of Environmental Management, Prince of Songkla University, Songkhla, Thailand.
The ability of white-rot fungus, AK04, to utilize phenolics as single and mixed substrates was determined in mineral medium and palm oil mill effluent (POME). The strain AK04 was able to rapidly metabolize all ten phenolics as single and mixed substrates at all test concentrations. With single substrates, between 78 and 98% removal was achieved within seven days.
View Article and Find Full Text PDFSci Rep
April 2020
Hamburg University of Technology, Institute of Technical Biocatalysis, Hamburg, 21073, Germany.
The Deepwater Horizon incident in the Gulf of Mexico in 2010 released an unprecedented amount of petroleum hydrocarbons 1500 meters below the sea surface. Few studies have considered the influence of hydrostatic pressure on bacterial community development and activity during such spills. The goal of this study was to investigate the response of indigenous sediment microbial communities to the combination of increased pressure, hydrocarbons and dispersant.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
March 2020
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, 10691, Stockholm, Sweden.
We report the first Fe -catalyzed biomimetic aerobic oxidation of alcohols. The principle of this oxidation, which involves several electron-transfer steps, is reminiscent of biological oxidation in the respiratory chain. The electron transfer from the alcohol to molecular oxygen occurs with the aid of three coupled catalytic redox systems, leading to a low-energy pathway.
View Article and Find Full Text PDFMar Pollut Bull
January 2020
Institute of Technical Biocatalysis, Hamburg University of Technology (TUHH), Hamburg, Germany. Electronic address:
A new Rhodococcus strain, capable of degrading crude oil, was isolated from the Gulf of Mexico deep-sea sediment and was investigated for its biodegradation characteristics under atmospheric as well as under deep-sea pressure (1500 m = 15 MPa). Additionally, the effect of dispersant (Corexit EC9500A) addition was studied. Rhodococcus sp.
View Article and Find Full Text PDFTalanta
November 2019
Institute of Technical Biocatalysis, Hamburg University of Technology, Denickestrasse 15, 21073, Hamburg, Germany. Electronic address:
The feasibility of Attenuated Total Reflectance Fourier Transform Infrared (ATR FTIR) spectroscopy and chemometric modeling for analysis of trace compounds is studied on the example of 5-(hydroxymethyl)furfural (HMF) in honey up to a concentration of 110 ppm, and compared to results in literature. Randomized repeated measurements of the calibration samples were carried out to prevent analytical artifacts. A partial least squares (PLS) model was calculated for quantitative determination.
View Article and Find Full Text PDFBioprocess Biosyst Eng
August 2019
DECHEMA Research Institute, Industrial Biotechnology, Theodor-Heuss-Allee 25, 60486, Frankfurt, Germany.
Deep eutectic solvents (DESs) have gained increased attention as alternative reaction media for biocatalysis in recent years. There are many investigations on biotransformations in a variety of DESs, but the purification of bioproducts from DES reaction mixtures has not yet been sufficiently addressed. The present study demonstrates a product recovery strategy from a DES reaction medium composed of (-)-menthol and dodecanoic acid.
View Article and Find Full Text PDFBiotechnol Rep (Amst)
June 2019
DECHEMA Research Institute, Industrial Biotechnology, Theodor-Heuss-Allee 25, 60486, Frankfurt a.M., Germany.
The lipase catalyzed esterification of (-)-menthol and lauric acid (LA) was studied in a eutectic mixture formed by both substrates((-)-menthol:LA 3:1, mol/mol). No additional reaction solvent was necessary, since the (-)-menthol:LA deep eutectic solvent (DES) acts as combined reaction medium and substrate pool. Therefore, the esterification is conducted under solvent-free conditions.
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