[Effect of light intensity on growth and quality of Asarum heterotropoides var. mandshuricum].

To study the infection rate of leaf spot disease, the drying rate of root and volatile oil content of Asarum heterotropoides var. mandshuricum at the unwrapping stage, blooming stage, the initial fruit stage, fructescence and wither stage during the growth period under the different sunlight intensity of 100% (I), 50% (II), 28% (III), 12% (IV). The volatile oil content was measured according to the method of Chinese Pharmacopoeia and the oil composition was determined by GC-MS.

Genetic evidence for domestic raccoon dog rabies caused by Arctic-like rabies virus in Inner Mongolia, China.

An outbreak of rabies affected domestic raccoon dogs on an animal farm in Inner Mongolia, China in 2007. A study was conducted to characterize the aetiological agent and clarify the origin of the rabies virus. Brain tissues were obtained from five rabid raccoon dogs.

[Study on allelopathic effect of ginseng root exudates against its root callus].

Objective: To study the effect of ginseng root exudates against its root callus and provide theoretic basis for allelopathic mechanism and continuous cropping obstacle.

Methods: We cultured ginseng root callus with MS culture medium whose concentration was equal to soil concentration of 80, 40, 20, 10, 5 gDW/mL and chose the optimum concentration for further study on allelopathic effect of ginseng root exudates against its root callus content of MDA and DNA. The root callus which was treated at different time was observed with paraffin slice method.

[Cloning, expression and antiviral activity of arctic fox (Alopex lagopus) interferon-gamma gene].

In order to characterize the biological activity of fox (Vulpes vulpes) interferon gamma(VuIFN-gamma), We have isolated the cDNA encoding arctic fox (Alopex lagopus) VuIFN-gamma. This cDNA encodes a 23 amino acid signal peptide and a 144 amino acid mature protein, which shares 99.8% or 99.

[Preparative separation of gentiopicrin from Gentiana scabra bunge by high speed counter current chromatography].

Objective: High speed counter current chromatography was successfully applied to the preparative separation of the component of Gentiana scabra Bunge.

Method: Gentiopicrin was obtained by preparative HSCCC with two phase solvent system com-posed of chloroform-methanol-tert-butylmethyl-water (5:4:2:4). The mobile phase is the lower phase and operated at a flow-rate of 2.