New and Interesting Taxa from the Diatom Genus Ehrenberg in Shallow, Nearshore Sites on the Eastern Coast of Lake Baikal.

During this investigation, sixteen species from the genus were found in a shallow bay (near Oimur, Kabansky District) located on the eastern shore of Lake Baikal. Eleven of these taxa have been described previously. Five species are described as new to science.

Changes in Structural and Thermodynamic Properties of Starch during Potato Tuber Dormancy.

The main reserve polysaccharide of plants-starch-is undoubtedly important for humans. One of the main sources of starch is the potato tuber, which is able to preserve starch for a long time during the so-called dormancy period. However, accumulated data show that this dormancy is only relative, which raises the question of the possibility of some kind of starch restructuring during dormancy periods.

Natural Deep Eutectic Solvents for the Extraction of Triterpene Saponins from var. (Rupr. & Maxim.) J. Wen.

The roots of the medicinal plant are rich in biologically active natural products, with triterpene saponins constituting one of their major groups. These metabolites can be efficiently extracted by methanol and ethanol. Due to their low toxicity, natural deep eutectic solvents (NADES) were recently proposed as promising alternative extractants for the isolation of natural products from medicinal plants.

First Insight into the Neuroprotective and Antibacterial Effects of Phlorotannins Isolated from the Cell Walls of Brown Algae and .

Phaeophyceae (brown algae) essentially contribute to biotopes of cold and temperate seas. Their thalli are rich in biologically active natural products, which are strongly and universally dominated with phlorotannins-polyphenols of complex and diverse structure based on multiple differently arranged phloroglucinol units and well known as strong antioxidants with a broad spectrum of biological activities. In the algal cells, phlorotannins can either accumulate in the cytoplasm or can be secreted into the cell wall (CW).

Antioxidant Status and Biotechnological Potential of New (Eustigmatophyceae) Soil Strains in Enrichment Cultures.

The functional state of enrichment cultures of the Eustigmatophycean strains MZ-E3 and MZ-E4 after 25-day cultivation in the BBM medium was studied. The concentrations of chlorophyll , total carotenoids, protein, vitamins A and E, fatty acid peroxidation product content, an antioxidant enzyme, and succinate dehydrogenase activity were measured. MZ-E3 succinate dehydrogenase activity was significantly higher by 2.

Phycobilisomes and Phycobiliproteins in the Pigment Apparatus of Oxygenic Photosynthetics: From Cyanobacteria to Tertiary Endosymbiosis.

Eukaryotic photosynthesis originated in the course of evolution as a result of the uptake of some unstored cyanobacterium and its transformation to chloroplasts by an ancestral heterotrophic eukaryotic cell. The pigment apparatus of Archaeplastida and other algal phyla that emerged later turned out to be arranged in the same way. Pigment-protein complexes of photosystem I (PS I) and photosystem II (PS II) are characterized by uniform structures, while the light-harvesting antennae have undergone a series of changes.

Effects of Vertically Heterogeneous Soil Salinity on Genetic Polymorphism and Productivity of the Widespread Halophyte .

Salinity is one of the environmental factors that affects both productivity and genetic diversity in plant species. Within the soil profile, salinity is a dynamic indicator and significantly changes with depth. The present study examined the effects of the vertical heterogeneity of soil salinity chemistry on the plant height, fresh and dry biomass accumulation, water content, level of genetic polymorphism, and observed and expected heterozygosity in seven populations of halophyte in natural habitats.

Chloride Channel Family in the Euhalophyte (L.) Pall: Cloning of Novel Members SaCLCa2 and SaCLCc2, General Characterization of the Family.

CLC family genes, comprising anion channels and anion/H+ antiporters, are widely represented in nearly all prokaryotes and eukaryotes. CLC proteins carry out a plethora of functions at the cellular level. Here the coding sequences of the SaCLCa2 and SaCLCc2 genes, homologous to Arabidopsis thaliana CLCa and CLCc, were cloned from the euhalophyte Suaeda altissima (L.

Cytokinin Modulates Responses to Phytomelatonin in under High Light Stress.

Fine-tuned interactions between melatonin (MT) and hormones affected by environmental inputs are crucial for plant growth. Under high light (HL) conditions, melatonin reduced photodamage in and contributed to the restoration of the expression of the cytokinin (CK) synthesis genes , and and genes for CK signal transduction , and , , and which were downregulated by stress. However, CK signaling mutants displayed no significant changes in the expression of CK genes following HL + MT treatment, implying that a fully functional cytokinin signaling pathway is a prerequisite for MT-CK interactions.

Detergent-Assisted Protein Digestion-On the Way to Avoid the Key Bottleneck of Shotgun Bottom-Up Proteomics.

Gel-free bottom-up shotgun proteomics is the principal methodological platform for the state-of-the-art proteome research. This methodology assumes quantitative isolation of the total protein fraction from a complex biological sample, its limited proteolysis with site-specific proteases, analysis of the resulted peptides with nanoscaled reversed-phase high-performance liquid chromatography-(tandem) mass spectrometry (nanoRP-HPLC-MS and MS/MS), protein identification by sequence database search and peptide-based quantitative analysis. The most critical steps of this workflow are protein reconstitution and digestion; therefore, detergents and chaotropic agents are strongly mandatory to ensure complete solubilization of complex protein isolates and to achieve accessibility of all protease cleavage sites.

Modeling and cleaning RNA-seq data significantly improve detection of differentially expressed genes.

Background: RNA-seq has become a standard technology to quantify mRNA. The measured values usually vary by several orders of magnitude, and while the detection of differences at high values is statistically well grounded, the significance of the differences for rare mRNAs can be weakened by the presence of biological and technical noise.

Results: We have developed a method for cleaning RNA-seq data, which improves the detection of differentially expressed genes and specifically genes with low to moderate transcription.

Phytochemical Characterization of Water Avens ( L.) Extracts: Structure Assignment and Biological Activity of the Major Phenolic Constituents.

Water avens ( L.) is a common plant widely spread in Europe and North America. It is rich in biologically active natural products, some of which are promising as prospective pharmaceuticals.

Growth and B-Phycoerythrin Production of Red Microalga (Porphyridiales, Rhodophyta) under Different Carbon Supply.

Red microalga (Bory) Drew is a well-known object of biotechnology due to its unique ability to synthesize a wide range of biologically active compounds. Enough minerals in an accessible form in a medium are a prerequisite for maintaining a high growth rate of . Carbon is the main element of microalgal biomass and is a component of all organic compounds.

Study of the Chemical Composition and Biologically Active Properties of Extracts.

or licorice has long been known as a commonly used Ayurvedic herb. This study aims to investigate the effect of extraction methods on the chemical composition and biologically active properties of extract samples. The highest yield of the extract (21.

Stem notching decreases stem hydraulic conductance but does not influence drought impacts and post-drought recovery in Scots pine and Norway spruce.

The tight connection between the deterioration of xylem function and plant mortality under drought is well recognized. However, a lack of mechanistic understanding of how substantial conductivity loss influences plant performance under drought and during post-drought recovery hinders our ability to model tree responses to drought stress. We artificially induced a loss of 50% of xylem conducting area in Scots pine and Norway spruce saplings by stem notching and investigated plant performance under drought and during post-drought recovery.

Molecular and Morphological Investigations of Two Giant Diatom Species from the Transbaikal Area (Russia, Siberia) with Comments on Their Distributions.

For the first time, a giant diatom species of the genus from Lake Baikal was studied using molecular methods. Molecular and morphological investigations allowed to us to described one new species, Glushchenko, Kulikovskiy and Kociolek sp. nov.

Study of the Antioxidant Properties of and .

The demographic situation of the last few decades is characterized by the increased numbers of elderly and senile people, i.e., by the aging of the population.

gen. nov.-A New, Neglected Monoraphid Diatom Genus with a Cavum.

A new monoraphid diatom genus, gen. nov., is described on the basis of a detailed morphological investigation using light and scanning electron microscopy.

Phylogeny and fatty acid profiles of Aliinostoc vietnamicum sp. nov. (cyanobacteria) from the soils of Vietnam.

A new cyanobacterial species of Aliinostoc, A. vietnamicum sp. nov.

Description of the Soil Diatom sp. nov. (Bacillariophyceae, Sellaphoraceae) from Vietnam, with Remarks on the Phylogeny and Taxonomy of and Systematic Position of .

In material isolated from soils of Cát Tiên National Park, we isolated four strains that were assigned to the genus . Identification was carried out on the basis of morphological and molecular studies. We proposed a new species named sp.

Phylogeny and Fatty Acid Profiles of New (Bacillariophyta) Species from Soils of Vietnam.

We studied the morphology, ultrastructure, and phylogeny of eight soil diatom strains assigned to the genus. Six of these strains, identified by us as new species, are described for the first time. We provide a comprehensive comparison with related species and include ecological data.

The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes.

Chloroplasts' mechanisms of adaptation to elevated temperatures are largely determined by the gene expression of the plastid transcription apparatus. Gene disruption of iron-containing superoxide dismutase PAP4/FSD3 and PAP9/FSD2, which are parts of the DNA-RNA polymerase complex of plastids, contributed to a decrease in resistance to oxidative stress caused by the prolonged action of elevated temperatures (5 days, 30 °C). Under heat stress conditions, pap4/fsd3 and pap9/fsd2 mutants showed a decline in chlorophyll content and photosynthesis level, as measured by photosynthetic parameters, and a different amplitude of HSP gene response to heat stress.

System analysis of the fast global coronavirus disease 2019 (COVID-19) spread. Can we avoid future pandemics under global climate change?

The recent fast global spread of COVID-19 caused by a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) questions why and how the disease managed to be so effective against existing health protection measures. These measures, developed by many countries over centuries and strengthened over the last decades, proved to be ineffective against COVID-19. The sharp increase in human longevity and current transport systems in economically developing countries with the background of persisting cultural frameworks and stable local pools of high bacterial and viral mutations generated the wide gap between the established health protection systems and the new emerging diseases.