Characterization of the oxygen-tolerant formate dehydrogenase from .

Fixation of CO into the organic compound formate by formate dehydrogenases (FDHs) is regarded as the oldest autotrophic process on Earth. It has been proposed that an FDH-dependent CO fixation module could support CO assimilation even in photoautotrophic organisms. In the present study, we characterized FDH from (FDH) due to its ability to reduce CO under aerobic conditions.

Continuous cropping of Patchouli alters soil physiochemical properties and rhizosphere microecology revealed by metagenomic sequencing.

Continuous cropping (CC) profoundly impacts soil ecosystems, including changes in soil factors and the structure and stability of microbial communities. These factors are interrelated and together affect soil health and plant growth. In this research, metagenomic sequencing was used to explore the effects of CC on physicochemical properties, enzyme activities, microbial community composition, and functional genes of the rhizosphere soil of patchouli.

Utilizing FRET-based Biosensors to Measure Cellular Phosphate Levels in Mycorrhizal Roots of .

Arbuscular mycorrhizal (AM) fungi engage in symbiotic relationships with plants, influencing their phosphate (Pi) uptake pathways, metabolism, and root cell physiology. Despite the significant role of Pi, its distribution and response dynamics in mycorrhizal roots remain largely unexplored. While traditional techniques for Pi measurement have shed some light on this, real-time cellular-level monitoring has been a challenge.

Multi-environmental traits selection and farmer's participatory assessment of mean performance and stability of orange-fleshed sweet potato genotypes in Benin.

Regularly consuming orange-fleshed sweet potatoes (OFSP) is widely recognised as an effective way to treat vitamin A deficiency (VAD), particularly in low-income countries. Unfortunately, cultivars of OFSP are poorly disseminated in most countries in sub-Saharan Africa, where VAD is a major cause of blindness. This study was conducted to evaluate the effect of the genotype-environment interaction (GEI) on the performance and stability of the yield components of OFSP cultivars to trigger their adoption by farmers.

Resistance haplotypes to green rice leafhopper ( Uhler) estimated in genome-wide association study in Myanmar rice landraces.

Green rice leafhopper (GRH, Uhler) is a serious insect pest of rice in the temperate regions of Asia. Myanmar has a high genetic diversity and is located at the center of the origin of rice. To understand the genetic architecture of GRH resistance in Myanmar rice landraces, a genome-wide association study (GWAS) was performed using a diversity panel collected from diverse geographical regions.

Comparative transcriptome profiling reveals the mechanism of increasing lysine and tryptophan content through pyramiding , and genes in maize.

To explore the molecular mechanism behind maize grain quality and use of different gene stacking to improve the nutritional quality of grain, marker-assisted selection (MAS) was used to select three recessive mutant lines containing , along with the double-recessive mutant lines containing , , and . The resulting seeds were taken for transcriptome sequencing analysis 18 days after pollination (DAP). Results: Compared with the recurrent parent genes, in the lysine synthesis pathway, the gene pyramiding lines (, , and ) revealed that the gene encoding aspartate kinase (AK) was up-regulated and promoted lysine synthesis.

Corrigendum to "Panax notoginseng saponins promotes angiogenesis after cerebral ischemia-reperfusion injury"[J Ginseng Res 48(6), November 2024, 592-602].

[This corrects the article DOI: 10.1016/j.jgr.

Targeting the DNA damage response (DDR) of cancer cells with natural compounds derived from and other plants.

DNA damage is a driver of cancer formation, leading to the impairment of repair mechanisms in cancer cells and rendering them susceptible to DNA-damaging therapeutic approaches. The concept of "synthetic lethality" in cancer clinics has emerged, particularly with the use of PARP inhibitors and the identification of DNA damage response (DDR) mutation biomarkers, emphasizing the significance of targeting DDR in cancer therapy. Novel approaches aimed at genome maintenance machinery are under development to further enhance the efficacy of cancer treatments.

Improved plant biomass production under low nitrogen conditions through conditional accumulation of the second messenger, guanosine tetraphosphate, in chloroplasts and mitochondria.

To enhance plant biomass production under low nitrogen conditions, we employed a method to artificially and temporarily accumulate the bacterial second messenger, guanosine tetraphosphate (ppGpp), to modify plastidial or mitochondrial metabolism. Specifically, we fused a chloroplast or mitochondrial transit-peptide to the N-terminus of the bacterial ppGpp synthase YjbM, which was conditionally expressed by an estrogen-inducible promoter in . The resulting recombinant plants exhibited estrogen-dependent ppGpp accumulation in chloroplasts or mitochondria and showed reduced fresh weight compared to wild type (WT) plants when grown on agar-solidified plates containing a certain amount of estrogen.

Unlocking biochar impacts on abiotic stress dynamics: a systematic review of soil quality and crop improvement.

Global agricultural challenges, especially soil degradation caused by abiotic stresses, significantly reduce crop productivity and require innovative solutions. Biochar (BC), a biodegradable product derived from agricultural and forestry residues, has been proven to significantly enhance soil quality. Although its benefits for improving soil properties are well-documented, the potential of BC to mitigate various abiotic stresses-such as drought, salinity, and heavy metal toxicity-and its effect on plant traits need further exploration.

Improving panicle blast resistance and fragrance in a high-quality rice variety through breeding.

Introduction: Huruan1212 (HR1212) is well-regarded for its superior eating and cooking quality in the lower reaches of the Yangtze River in China. Still, its high susceptibility to rice panicle blast and lack of fragrance have limited its further spread and utilization. and are two dominant genes known for their stable broad-spectrum resistance against rice blast fungus , while is the crucial gene that regulates rice aroma.

Fade into you: genetic control of pigmentation patterns in red-flesh apple ().

The genetic basis of type 1 red-flesh color development in apple () depends upon a particular allele of the gene. Interestingly, type 1 red-flesh apples are fully red after fruit set, but anthocyanin pigmentation in apple fruit cortex may decrease during fruit growth and maturation, leading to variable red patterning and intensities in the mature cortical flesh. We developed a histogram-based color analysis method to quantitatively estimate pigmentation patterns.

Long-term nitrogen fertilization alters the partitioning of amino acids between citrus leaves and fruits.

Introduction: The growth of evergreen fruit trees is influenced by the interaction of soil nitrogen (N) and leaf amino acid contents. However, information on free amino acid contents in leaves of fruiting and non-fruiting branches during long-term N fertilizer application remains scarce.

Methods: Here, a four-year field experiment (2018-2021) in a citrus orchard revealed consistently lower total N and amino acid contents in leaves of fruiting compared to non-fruiting branches.

Drought as an emergent driver of ecological transformation in the twenty-first century.

Under climate change, ecosystems are experiencing novel drought regimes, often in combination with stressors that reduce resilience and amplify drought's impacts. Consequently, drought appears increasingly likely to push systems beyond important physiological and ecological thresholds, resulting in substantial changes in ecosystem characteristics persisting long after drought ends (i.e.

Cotton leaf curl Multan virus activates autophagy in the whitefly AsiaII7, weakening its vectorial capacity for transmission.

Background: Autophagy plays an important role against pathogen infections in both insects and plants. Insect vectors employ autophagy as an intrinsic antiviral defense mechanism against viral infections, whereas viruses can exploit autophagy to enhance their transmission via insect vectors. The Cotton leaf curl Multan virus (CLCuMuV) is transmitted by the AsiaII7 cryptic species of Bemisia tabaci, however, the role of autophagy is involved in regulating the transmission of this virus remains unclear.

Effects of Conservation Agriculture on Soil NO Emissions and Crop Yield in Global Cereal Cropping Systems.

Conservation agriculture, which involves minimal soil disturbance, permanent soil cover, and crop rotation, has been widely adopted as a sustainable agricultural practice globally. However, the effects of conservation agriculture practices on soil NO emissions and crop yield vary based on geography, management methods, and the duration of implementation, which has hindered its widespread scientific application. In this study, we assessed the impacts of no-tillage (NT), both individually and in combination with other conservation agriculture principles, on soil NO emissions and crop yields worldwide, based on 1270 observations from 86 peer-reviewed articles.

Uptake, Subcellular Distribution, and Metabolism of Decabromodiphenyl Ethane in Vegetables under Different Exposure Scenarios.

Decabromodiphenyl ethane (DBDPE), a key alternative to deca-BDE (BDE-209), has been ubiquitous in the receiving ecosystem. However, little is known about its uptake process and fate in plants. Here, the plant absorption, distribution, and metabolism of C-DBDPE under two distinct exposure pathways (i.

Flower colour contrast, 'spectral purity' and a red herring.

Nature offers a bewildering diversity of flower colours. Understanding the ecology and evolution of this fantastic floral diversity requires knowledge about the visual systems of their natural observers, such as insect pollinators. The key question is how flower colour and pattern can be measured and represented to characterise the signals that are relevant to pollinators.

Graph-based pangenome provides insights into the structural variation and genetic basis of metabolic traits in potato.

Potato is the world's most important nongrain crop. Here, we report that 29 genomes from Petota and Etuberosum sections were de novo assembled, and that 248 accessions of wild potatoes, landraces and modern cultivars were re-sequenced at > 25× depth to assess genetic diversity within the Petota section. Subsequently, a graph-based pangenome was constructed by using DM8.

High-Efficiency CRISPR-Cas9 Genome Editing Unveils Biofilm Insights and Enhances Antimicrobial Activity in Bacillus velezensis FZB42.

Bacillus velezensis FZB42 is a prominent plant growth-promoting rhizobacterium and biocontrol agent known for producing a wide array of antimicrobial compounds. The capability to genetically manipulate this strain would facilitate understanding its metabolism and enhancing its sustainable agriculture applications. In this study, we report the first successful implementation of high-efficiency CRISPR-Cas9 genome editing in B.

Differential responses of root and leaf-associated microbiota to continuous monocultures.

Continuous monocultures alter the composition and function of root-associated microbiota, and thus compromise crop health and productivity. In comparison, little is known about how leaf-associated microbiota respond to continuous monocultures. Here, we profiled root and leaf-associated microbiota of peanut plants under monocropping and rotation conditions.

S100A13-driven interaction between pancreatic adenocarcinoma cells and cancer-associated fibroblasts promotes tumor progression through calcium signaling.

Background: Cancer-associated fibroblasts (CAFs) are key components of the pancreatic adenocarcinoma (PAAD) tumor microenvironment (TME), where they promote tumor progression and metastasis through immunosuppressive functions. Although significant progress has been made in understanding the crosstalk between cancer cells and CAFs, many underlying mechanisms remain unclear. Recent studies have highlighted the importance of calcium signaling in enhancing interactions between tumor cells and the surrounding stroma, with the S100 family of proteins serving as important regulators.

New job, new habits? A multilevel interrupted time series analysis of changes in diet, physical activity and sleep among young adults starting work for the first time.

Background: The workplace is an important determinant of health that people are exposed to for the first-time during adolescence or early adulthood. This study investigates how diet, physical activity, and sleep change as people aged 16-30 years transition into work and whether this varies for different individuals and job types.

Methods: Multilevel linear regression models assessed changes in fruit and vegetable intake, sleep duration, and physical activity among 3,302 UK Household Longitudinal Study (UKHLS) participants aged 16-30 years, who started work for the first time between 2015 and 2023.

Essential oils from Amorpha fruticosa against hepatocellular carcinoma based on network pharmacology.

Background: Amorpha fruticosa was used for treating burn, ambustion, carbuncle, and eczema in the traditional Chinese medicine. Although more and more attention has been paid to its biological activity recently, the antitumor activities of the essential oils (EOs) extracted from its leaves (AFLEO) and flowers (AFFEO), and their molecular mechanisms have never been reported up to now. The objective of present study was to examine the chemical compositions of AFLEO and AFFEO, then investigate the effects and pharmacological mechanism of EOs against hepatocellular carcinoma (HCC).