Effects of secretory nerve stimulation on the movement of rat parotid amylase into the circulation.

Stimulation of the nerves to the rat parotid gland demonstrated that increases in serum amylase levels can originate exclusively from this gland. However, whilst parasympathetic nerve stimulation caused a significant movement of parotid amylase into the blood, sympathetic nerve stimulation did not, despite the higher concentration of amylase in the saliva evoked. Experiments in which conscious animals were fed for a short period showed that parotid amylase reaches the blood during normal activity.

Parasympathetic activation of amylase secretion in the intact and sympathetically denervated rat parotid gland.

Observations were made on amylase secretion from the rat parotid gland induced by parasympathetic nerve stimulation in vivo. In spite of a low salivary amylase concentration a large total amylase output, equivalent to 36% of initial gland content, was found on prolonged activation (5 Hz, 120 min). However, no decrease in gland amylase content was found, which indicates that the output of amylase was balanced by synthesis.

Stimulation of cat cutaneous nociceptive C fibres causing tonic and synchronous activity in climbing fibres.

1. The input from cutaneous nociceptors to climbing fibres projecting to the forelimb area of the C3 zone in the cerebellar anterior lobe was examined in barbiturate-anaesthetized cats. Climbing fibre responses were simultaneously recorded in single Purkinje cells and as field potentials from the cerebellar surface close to these cells.

Climbing fibres projecting to cat cerebellar anterior lobe activated by cutaneous A and C fibres.

1. Climbing fibre responses evoked on stimulation of the ipsilateral superficial radial nerve were examined in the forelimb area of the C3 zone in the barbiturate-anaesthetized cat. Climbing fibre responses were recorded in sixty-five Purkinje cells and as field potentials from the surface of the cerebellum.

Amylase-secretory responses to sympathetic nerve impulses in the rat parotid gland following partial sympathetic ganglionectomy.

Some sympathetic axons reach the parotid gland from the contralateral sympathetic chain. Such contralateral nerves were stimulated and saliva secreted after intravenous injections of methacholine was collected from the parotid duct at various times after removal of the ipsilateral superior cervical ganglion. The secretion of amylase caused by sympathetic nerve stimulation was greatly increased three days after the sympathectomy and even more after ten weeks.