212 results match your criteria: "Institute of Pathology ICMR[Affiliation]"

Atherosclerosis is increasingly recognized as a chronic inflammatory disease. A variety of infectious agents (Chlamydia pneumoniae, Helicobacter pylori, and cytomegalovirus [CMV]) and inflammatory marker such as high-sensitivity C-reactive protein (hs-CRP) have been found to be associated with atherosclerosis and its consequences. There is a need to know about the type and burden of infection in coronary artery disease (CAD) patients and the level of hs-CRP in India as there is growing evidence that a variety of pathogens are participating in the development and/or acceleration of at least pre-existing atherosclerosis.

View Article and Find Full Text PDF

Little is known about genital mucosal immune response to chlamydial infection in women with or without sequelae (Chlamydia positive women with or without fertility disorders as infertility and multiple spontaneous abortions). Cervical lymphocytes were stimulated with chlamydial EBs and cytokine secretion was determined by ELISA, RT-PCR and ELISPOT assays. Stimulated cervical cells from women with fertility disorders (FD) secrete significantly (P<0.

View Article and Find Full Text PDF

Objectives: A strong association of cytokines [interleukin (IL)-6, IL-2 and tumor necrosis factor (TNF)-alpha] with atherosclerosis is well documented. However, their role in Chlamydia pneumoniae (Cp)-associated progression of coronary artery disease (CAD) is not well studied. Hence we evaluated the presence of these cytokines in CAD patients positive for Cp immunoglobulin (Ig) A (low, medium, and high levels) CAD and compared with controls.

View Article and Find Full Text PDF

Natural rubber latex allergy.

Indian J Dermatol Venereol Leprol

February 2009

Institute of Pathology (ICMR), New Delhi, India.

Natural rubber latex (NRL) is a ubiquitous allergen as it is a component of > 40,000 products in everyday life. Latex allergy might be attributed to skin contact or inhalation of latex particles. Latex allergy is an IgE-mediated hypersensitivity to NRL, presenting a wide range of clinical symptoms such as angioedema, swelling, cough, asthma, and anaphylactic reactions.

View Article and Find Full Text PDF

Lymphoplasmacyte-rich meningioma at the foramen magnum.

Br J Neurosurg

October 2008

Institute of Pathology-ICMR, Safdarjung Hospital, New Delhi, India.

We report a case of lymphoplasmacyte-rich meningioma (LPM) in a 22-year-old woman who presented with headache, hemiparesis and hypoaesthesia. MRI revealed a meningioma at the foramen magnum region that was resected by far-lateral approach. This case represents the second LPM to be reported at this site and highlights the importance of distinguishing it from other lesions that may have significant population of lymphocytes and plasma cells.

View Article and Find Full Text PDF

We report a case of post-kala-azar dermal leishmaniasis in which real-time PCR was exploited to measure changes in cytokine transcripts in lesion tissue before and after oral miltefosine treatment. Unlike antimonial therapy elevated levels of IFNgamma transcripts were noted, whereas TNFalpha, IL-10 and transforming growth factor-beta declined similar to that observed after therapy with antimonials. A significant increase in IFNgamma and CD40 levels seen after miltefosine therapy could enhance parasite clearance.

View Article and Find Full Text PDF

Tandem use of immunofluorescent and DNA staining assays to validate nested PCR detection of mycoplasma.

In Vitro Cell Dev Biol Anim

October 2008

Cell Biology, Institute of Pathology (ICMR), P. B. No. 4909, Safdarjang Hospital Campus, New Delhi, Delhi 110029, India.

Mycoplasma contamination in cell culture is a serious setback to cell culturists across the world with a very high rate of reported occurrence particularly because of difficult early detection. Out of a variety of detection methods known, the double-step nested polymerase chain reaction (PCR)-based detection of mycoplasma in cell culture has been critically viewed upon because of chances of producing reliable results. A nested PCR technique, described to detect a large range of cell-culture-contaminating mycoplasma species, with greater sensitivity to detect as low a contamination as a few organisms, was compared with the results from two cytological techniques employed in tandem.

View Article and Find Full Text PDF

Background: The magnitude of reproductive morbidity associated with sexually transmitted Chlamydia trachomatis infection is enormous. Association of antibodies to chlamydial heat shock proteins (cHSP) 60 and 10 with various disease sequelae such as infertility or ectopic pregnancy has been reported. Cell-mediated immunity is essential in resolution and in protection to Chlamydia as well as is involved in the immunopathogenesis of chlamydial diseases.

View Article and Find Full Text PDF

Tissue microarray (TMA) technology is a high-throughput research tool, which has greatly facilitated and accelerated tissue analyses by in-situ technologies. TMAs are amenable to every research method that can be applied on the standard whole sections at enhanced speed. It plays a central role in target verification of results from cDNA arrays, expression profiling of tumors and tissues, and is proving to be a powerful platform for proteomic research.

View Article and Find Full Text PDF

Visceral leishmaniasis (VL) or Kala azar (KA) is a systemic disease caused by the parasites of the Leishmania donovani complex. Control measures rely on treatment with antileishmanial agents, however, fraught with problems such as toxicity or drug resistance. The incidence rate is on the rise for children, for reasons yet undefined.

View Article and Find Full Text PDF

Subcutaneous panniculitis-like T cell lymphoma (SPTCL) is a rare cytotoxic T-cell lymphoma classified in the World Health Organization-European Organization for Research and Treatment of Cancer (WHO-EORTC) classification as a unique extranodal lymphoma with characteristic by T cell receptor (TCR) gene rearrangement. We report here a case of SPTCL in a 22 year-old woman who had presented with variably sized multiple nodules on both her legs. Initial differential diagnoses considered were panniculitis and lupus panniculitis.

View Article and Find Full Text PDF

The diagnostic potential of recombinant Lepp12 (rLepp12) antigen cloned from Leishmania infantum was assessed in L. donovani infections by Western blotting. Ninety-two serum samples, including 30 patients with active kala-azar (KA), 17 post-treated KA patients (KA-PT), 20 post-kala-azar dermal leishmaniasis (PKDL) patients and 25 controls, were analysed for rLepp12, rK39 and DAT positivity.

View Article and Find Full Text PDF

Nepal is endemic for visceral leishmaniasis; however, recently a solitary report of cutaneous leishmaniasis (CL) was recorded. The present report describes a CL case in a Nepalese woman, initially referred as a case of basal cell carcinoma. Histopathological examination, culture and PCR analysis of lesions revealed it to be a case of CL.

View Article and Find Full Text PDF

To increase the sensitivity and reliability of immunodiagnostic assay for direct detection of chlamydial genital infection, we developed monoclonal antibodies (MAbs) that recognize the major outer membrane protein (MOMP) of all serovars of Chlamydia trachomatis. ELISA and dot-ELISA were used to select hybridomas that produced anti-C. trachomatis MAbs.

View Article and Find Full Text PDF

Post-kala-azar dermal leishmaniasis (PKDL) is an unusual dermatosis that develops as a sequel in 5-15% of cured cases of kala-azar (KA) after months or years of treatment in India. Molecular differences are reported to exist between the KA and PKDL isolates which may underlie the diversity in clinical manifestations of the disease. Here, arbitrary primed-PCR (AP-PCR) has been used for genetic fingerprinting of parasite isolates from dermal lesions of PKDL patients (n=14) and compared with bone-marrow derived parasites from KA patients (n=3).

View Article and Find Full Text PDF

Short communication: post-kala-azar dermal leishmaniasis--an appraisal.

Trop Med Int Health

July 2007

Department of Dermatology & Regional STD Centre, and Institute of Pathology (ICMR), Safdarjang Hospital and V.M. Medical College, New Delhi, India.

This short descriptive paper assesses diagnostics, clinical characteristics and therapy of both Indian and African post-kala-azar dermal leishmaniasis.

View Article and Find Full Text PDF

Background: There is growing evidence that Chlamydia pneumoniae may be involved in the pathogenesis of atherosclerosis, as several studies have demonstrated the presence of the organism in atherosclerotic lesions. C. pneumoniae infections, which are especially persistent infections, have been difficult to diagnose either by serological methods or isolation of the organism from the tissue.

View Article and Find Full Text PDF

Myxoid neurothekeoma is a rare benign cutaneous neoplasm of nerve sheath origin and is also called as benign nerve sheath myxoma. This entity is seen in head, neck and upper extremity and seldom on the lower limb. A myxoid neurothekeoma in the lower leg of a 33-year-old woman is described.

View Article and Find Full Text PDF

Identification of new foci of cutaneous leishmaniasis (CL), along with reports of Leishmania donovani causing the disease, is an issue of concern. Clinico-epidemiologic analysis of 98 cases in the endemic regions of Rajasthan state, India, suggested the preponderance of infection in men (62.24%) compared with women (37.

View Article and Find Full Text PDF

Effectiveness of a mycoplasma elimination reagent on a mycoplasma-contaminated hybridoma cell line.

Hybridoma (Larchmt)

April 2007

Cell Biology Laboratory, Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, India.

Mycoplasma contamination in cell culture is a frequently occurring serious limitation to biomedical research, particularly when it affects the irreplaceable cell lines. Although there are few reports of its successful elimination through rigorous protocols, it is the usual practice to destroy the infected cultures. Lately, a physical method using a mycoplasma-eliminating surfactin was described to effectively eliminate mycoplasma contamination from infected cell lines upon single use.

View Article and Find Full Text PDF

Little is known about concurrent expression of cervical cytokines and their regulation by sex hormones during primary or recurrent chlamydial infections in humans. Cytokine (interleukin-1beta [IL-1beta], IL-6, IL-10, interferon-gamma [IFN-gamma], and tumor necrosis factor-alpha [TNF-alpha]) concentrations in cervical washes and serum samples, along with levels of beta-estradiol and progesterone in women with primary or recurrent chlamydial infections and healthy controls, were measured by ELISA. Women with recurrent infections had significantly higher levels of IFN-gamma in cervical washes than did women with primary infections.

View Article and Find Full Text PDF

Background: Papillary meningiomas are rare meningeal tumors and are associated with aggressive clinical behavior as compared with other meningiomas. Because of their rare occurrence, they may pose a diagnostic dilemma to the unwary pathologist. We report a case of papillary meningioma in a 16-year-old boy.

View Article and Find Full Text PDF

Interferon gamma (IFN-gamma) and nitric oxide (NO) are the major players of the host defense against Leishmania. In the present study circulating levels of IFN-gamma, NO, interleukin (IL)-6 and C-reactive protein (CRP) were compared in kala azar (KA), post-kala azar dermal leishmaniasis (PKDL) and healthy controls. A significantly elevated level of these parameters was evident in KA compared to PKDL or control.

View Article and Find Full Text PDF

Problem: Chlamydial infections are often associated with various fertility-related disorders. Serological prediction of these has limitations, as they do not differentiate between past and current infections. Thus, we looked for local markers that could predict more precisely women at higher risk of developing severe complications.

View Article and Find Full Text PDF

Background: Clear cell odontogenic carcinoma is a rare odontogenic tumor occurring in the anterior region of the mandible in 5th-7th decades and shows a female preponderance. It is potentially aggressive, capable of frequent recurrences and loco-regional and distant metastases.

Case Presentation: A 45-year-old woman presented with a radiolucent left mandibular swelling associated with loss of teeth.

View Article and Find Full Text PDF