Detection of Trichinella spiralis antigens in urine of men and animals.

The practical inability to diagnose Trichinella spiralis antibodies in man before day 20 post infection (dpi) has stimulated interest in the development of immunodiagnostic test to detect circulating antigens. Our previous experience showed that soon after infection immune complexes as well as uncomplexed parasite antigens in sera of infected rats could be detected. To diagnose the presence of antigen in urine, double sandwich-capture ELISA was applied using a peroxidase-conjugated rabbit immunoglobulin to T.

Occurrence of Strongylidae (Nematoda: Strongyloidea) in Polish horses "tarpans" from Popielne Reserve.

The study was performed to evaluate intensity of strongylid infection in stabled tarpans using the method of collecting worms after anthelmintic treatment and in wild tarpans from Popielne reserve on the basis of faecal examination. After treatment with ivermectin 12 cyathostomes and one large strongyle species were recovered from the faeces of two tarpans. The most abundant cyathostome species were C.

Prevalence of Toxocara canis infection in dogs in the Warszawa area.

The evaluation of Toxocara canis infection in stray dogs from two shelters and private owners dogs in the Warszawa district was the aim of this study. In 1998 five hundred faecal samples were examined. The homeless dogs were found more infected than those kept as pets.

The contamination of the environment with Toxocara eggs in Mazowieckie voivodship as a risk of toxocarosis in children.

The home surroundings (109 places) of 120 children with diagnosed toxocarosis were examined to assess if the presence of Toxocara spp. eggs in the soil corresponds with the occurrence of clinical cases. 31 places (28.

Trichinella spiralis: macrophage activity and antibody response in chronic murine infection.

The role of macrophages, their products, and the specific antibody response were examined during chronic Trichinella spiralis infection in BALB/c mice. Adult T. spiralis in intestines were detected from 5 to 20 dpi.

Calcification of Trichinella spiralis larval capsule.

Trichinella spiralis larvae were examined in TEM to identify calcareous corpuscles in the outer part of parasite capsule. The microroentgenographic analysis of calcareous corpuscles mainly demonstrated the presence of phosphorus and calcium. The physiological importance of calcareous corpuscles, as well as their significance in decay of T.

Antibodies to Neospora caninum in the blood of European bison (Bison bonasus bonasus L.) living in Poland.

The prevalence of antibodies to Neospora caninum was examined in European bison (Bison bonasus bonasus L.) living in free and fenced areas in Poland. Sera of 320 European bison, different ages and sexes, from breeding areas in Poland were tested for N.

Reduction of muscle larvae burden in rats experimentally infected with Trichinella spiralis.

In Wistar rats infected with 500 to 2,500 Trichinella spiralis larvae the muscle larvae intensity (larvae per gram-l.p.g.

Factors conditioning detection of Taenia saginata antigens in faeces.

The conditions which optimize detection of T. saginata antigens in faeces were subject of examination. The most appropriate appeared diluent of PBS + 0.

Detection of Taenia saginata antigens in faeces by ELISA.

Stool samples were examined by capture ELISA for the presence of T. saginata antigens. The specimens were from 303 patients infected with T.

The prevalence and abundance of internal parasites in working horses autopsied in Poland.

The digestive tracts of 50 working horses from private farms in Poland were examined. Thirty-seven nematode species, two tapeworm species and one species of botfly were recovered. The most prevalent small strongyle species were Cyathostomum catinatum, Cylicocyclus nassatus, Cylicostephanus goldi, Cylicostephanus longibursatus, Cyathostomum coronatum, Cylicostephanus calicatus, Cylicocyclus leptostomus and Cylicostephanus minutus.